Nanodrop Ratios Explained | 260/280 ratio DNA | 260/280 ratio RNA | 260/230 Ratio | Qunatification |

Nanodrop Ratios Explained | 260/280 ratio DNA | 260/280 ratio RNA | 260/230 Ratio | Quantification |
This video lecture explains
1. The principle of Nanodrop instrumentation
2. Ideal 260/280 ratio for DNA and RNA concentration Measurements
3. Ideal 260/230 ratio for DNA and RNA concentration measurement
4. Absorption maxima for DNA, RNA, Proteins, and Salts
5. Real-world examples of RNA and RNA quantification using Nanodrop Instrumentation
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Disclaimer
This video is for educational purposes only. Copyright Disclaimer Under Section 107 of the Copyright Act 1976, allowance is made for "fair use" for purposes such as criticism, comment, news reporting, teaching, scholarship, and research. Fair use is a use permitted by copyright statute that might otherwise be infringing. Non-profit, educational, or personal use tips the balance in favor of fair use.
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[@easybiologynotes]

Very well explained about the basics of ratios for RNA and DNA quantification

[@BiologyLectures]

thanks

[@investincrypto2859]

Very good explanation about nanodrop ratios for DNA RNA quantification

[@BiologyLectures]

thanks

[@reyhaneesmaieli1117]

you explained it so well. thank you

[@BiologyLectures]

You're very welcome!

[@turtle-jelly]

I understand this perfectly! Thank you!

[@BiologyLectures]

You are most welcome 🤗

[@janhvi143]

very detailed & just what I needed! thank you

[@BiologyLectures]

You're so welcome!

[@dikshyapanthi7681]

Excellent lecture you n TH-cam about nanodrop ratios

[@BiologyLectures]

thanks

[@mohamadhasanansarizadeh7420]

Thanks, simple and informative, AND CLEAR

[@BiologyLectures]

Thanks a lot. Your comment means a lot to us. It is highly motivating for us.

[@michellesuelo7581]

hello! may I ask what software you used to graph that?

[@vkings4life]

Thanks alot for this

[@BiologyLectures]

Glad it helped

[@markrobinson9676]

Excellent explanation sir 😊

[@BiologyLectures]

thanks

[@MohdFaisal-ps7df]

If we have contamination in our extracted DNA or RNA and we don't have extra sample for the extraction again, what should we do in this case how we can decontaminate our extracted DNA?

[@BiologyLectures]

Enzymes such as DNase or RNase can be used to degrade contaminating DNA or RNA, respectively. If the contaminant is the opposite nucleic acid type (e.g., DNA contaminating an RNA sample), using the appropriate enzyme can help degrade the contaminant while leaving the target nucleic acid relatively intact.

[@MohdFaisal-ps7df]

@@BiologyLectures Thanks for the response, i know this but my question is how we will perform this on already extracted DNA or RNA is there any method to perform this, I hope you you don't mind
Thank you

[@BiologyLectures]

@@MohdFaisal-ps7df You can perform DNAse or RNAse treatment on already extracted DNA or RNA. Just take already started DNA or RNA and use DNAse or RNAse and corresponding buffer. I hope this helps.

[@akankshyapattanayak9469]

Thank You Sir for explaining .
Sir, If question raises,,, What is 260/280 ratio
...... Can we answer that 260 is for DNA and 280 for protein..
Please reply