I put my DNA under the microscope and you can too 🔬 Easy DNA extraction
ฝัง
- เผยแพร่เมื่อ 22 ธ.ค. 2024
- I show you how to extract DNA and how to put it under the microscope. Individual DNA molecules are actually too thin to be seen under the microscope. The fibers that I found are probably many DNA molecules stuck together with proteins and other cell material sticking to it.
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This is the type of Science I wanted to learn in school
You have to take genetic engineering as a career
Me too, now im grade 10 we are learning about gender 127
Exactly you are right I am in class 11 now
@Scott Pickering how is this "basic" science? because he made it seem easy using common materials?
@Scott Pickering
@Scott Pickering
in what reality? hopefully the one where actual scientific thinking applies
what "basic science" do you figure out what is or is not dna in a pool of stuff? how do you know what breaks down proteins and cells to get at dna? how do you know how to separate dna from other things? can you explain the "basic" science to get at all of the information being presented for each step?
So, since its "common materials" it's "basic"? regardless of how many non-"basic" sciences use common materials in a lot of their processes.
"its looking at stuff through a microscope".... this is like saying "its looking at stuff using a calculator/computer" for rocket science....
If you just "looked at stuff through a microscope", would you know what you are looking at at all?
Keep in mind how he can't verify that these things are indeed dna or not, or if the clumping is from protein or not; he's making educated guesses based on "basic" science?
Do you think "astrophysics or rocket science" can't have small inaccurate experiments like this that make things seem "basic" because it's not a "basic science"?
How exactly do you define "basic" science, because I don't see a consistent approach being used here.
It's likely an aggregate of supercoiled DNA, the 300nm loops I think would still be way too small. And if you recently ate something especially with starches, you might be seeing a lot of alpha-amylase proteins! We did some similar things in my Biotechnology lab.
I think this a great activity btw, it's a really good basic example of DNA extraction without needing purification columns and lab grade detergents
It could be condensed chromatin fibers, which is roughly 700 nm wide. That could be seen with a light microscope I think.
why do we see alpha-amylase proteins after eating starch?
@@tannukashyap8311 Looking back on this you wouldn't be able to actually see the proteins like that, too small. I think I was assuming there is an increase in alpha amylase enzyme production in your mouth during starch consumption to help increase digestion, but I don't actually know if that's true
@@SystemUpdate310 I think it's possible to see 700nm in a light, and yes it is condensed chromatin fibers, heterochromatin is 700
@@tannukashyap8311 they are enzymes used in the digestion of starch
So if you eat a lot of starchy food, then your cells (Intestinal for this one in particular) would respond by synthesizing more alpha amylase enzymes
To help in the digestion of it
Amazing!!! I wish more people got interesested in content like this!!!!! Just fascinating, thank you very much
Totally magnificent dear
Exactly :)) people should show their more interest in science ...
Najma Samantha...Microscopy, and amateur astronomy are the best hobbies anyone can get into!..It sure beats coin, and stamp collecting which I find to be dull, and boring!
@@damontsekos365 hobbies are subjective. I find stamp and coin collecting boring as well but you can’t go around telling people what they should be more interested in
People are suprised that I’m 11 and I like science and not stupid video games
I am sure you can do fluorescence microscopy . You could use a DNA fluorochrome stain such as Acridine Orange to stain the DNA. Alternatives are natural DNA staining fluorochromes include Berberine (from Barberry - Berberis spp.), and extracts from Greater Celandine (Chelidonium majus) which is a common plant in many parts of the world, and Plume Poppy (German = Federmohn) (Macleaya sp). You don't specifically need UV for this fluorescence, because most of these stains can be excited with Blue light and emit a yellow gold color.
I am a young and new passionate science teacher! Glad I found your channel! I love learning the content you produce Sir
Such a genuine content .
Not the cringe as other creator
I wish this kind of proffesor are also present in pur college🌸🌸
Hai Apoorva were is our college in India
Academia has an agenda ,,,keep them dumb ,,,,not too dumb.Evil to the lying core.
Im learning a lot more with your videos than with my classes at the university. I've seen your gallery, it's fantastic, I use the pictures to compare what Im looking in the microscope. Your job is amazing
point of classes at university is to give you the background to allow you to get to these types of things on your own, designing and figuring things out.
just watching and learning specific things is mostly only beneficial for a particular job or task. it can be more motivating because its more interesting, but it should motivate you to learn the class stuff.
Then you must not be paying attention in your university classes...
Sir you could use lens cleaner as protease to degrade proteins (histone) that binds up dna and see in micro scope of high resolution you may get a better view of dna
The open source licensing s very honorable. Thank you very much!
Absolutely!! 100%
Sir in a very beautiful way you have shown DNA. It is really fascinating.
you should've used eye drops as they have cellulase and amylase and starch would be destroyed, more chances of actually seeing DNA. although i think the micrographs you got at the end, the little beads on string are actually DNA. you madman Oliver I love you you did it
Most excellent spit sample microscopy analysis. Whether or not we could see dna is compensated by impressive discussion of observed micro forms and structures. Bravo!
Man, it's awesome. Keep up the good work 👍🏻
what kind of microscope is neccessary to really see the DNA?
I just downloaded a PDF on doing the banana activity. Going to do it with my grandchildren. It was great to see how all was done, Thanks
Hello, I’m currently a freshman starting veterinary medicine and I’ve been looking for information on how “far” a microscope can let us observe. My question is, and I would appreciate if you help me answer it, could one observe the DNA of an animal thru the microscope? Other than bacteria and cells, what else can a microscope let us observe? Please and thank you.
Must admit the title and thumbmail got me it - "look at my DNA under the microscope" and the head of Microbehunter - I'm thinking, wrong website/hub. But no, it's educational, what a surprise.
I love your good vibes. TY for the teachings. Much love.
I had experienced and did many experiments in the high equipped lab while doing my post graduation in microbiology discipline... !
My researches included microbiology, biotechnology, biochemistry,cell biology and molecular biology... !
So you would say it is DNA that we are seeing?
I study b SC biotechnology 1st year. In my practical we do Isolation of DNA from both plant and animal tissue. it's gave a best results.
wow i was just asking myself where would i be able to find library of these mico friends .Thanks alot!
me too mate.
You have the best channel on microscopy, but you need more videos about vintage microscopes, vintage (and modern) staining methods, and diy staining (there's something on the internet, but I only believe in your recipes 😉).
Sir can we use sanitizer instead of Isopropyl alcohol
How much does the microscope cost and do you have a link for its website?
O my God! that was such an amazing experiment oliver.....Thank u so much..
Wow excellently cool experiment
Perhaps a silly question. Shouldn't the utensils and equipment be sterile?
Incredible video! Seeing any amount of DNA under a light microscope of all things is far better than nothing at all. Thank you for the tip.
However, can you go more into detail about this procedure? Never heard of this before and not too sure about the materials, for example if it is better to use 99% isopropyl alcohol or something much lower like 70%.
Many Thanks again!
I have used 96% (about) ethyl alcohol. It works better when the alcohol is ice cold, but I did not cool it and it also worked. The DNA participation with alcohol is standard procedure in molecular biology. The alcohol reduces the polarity of the water to the extent that DNA can not be dissolved anymore. Normally one would collect the DNA by centrifugation after the addition of alcohol.
I know that the DNA, which is an acronym for De-oxy-ribo-Nucleic-Acid, is a double helix structure with some tiny steps and phosphate sugars on it but, I don't think I can actually see those things under my personal microscope. What is the name of the detergent you made use of? Is it Persil, Tide or Gain?? Or did you make use of any common detergent? I'll try this experiment once again and make sure I get the right viewing under the microscope. BTW, this video was amazing! Thanks for sharing.🎆🎆😁😁😊
I don't have my microscope yet. It will be here Wednesday. But I still find y our videos fascinating. I had no idea one could isolate DNA like this. I guess I better start saving up for my very own Scanning Electron Microscope. :)
woah. That’s cool. Can’t wait for more content!
Can you please give me the measurements of all the things that you used? I tried it but nothing happened so I think my measurements were off.
I'm trying to do a project on this...your support would be very helpful!
I did not measure anything, i estimated. Spoon of salt in 100ml water, drop of liquid soap, 96% ethanol.
@@Microbehunter ok thanks so much!
Hey, it's been about three months since you posted this video. Any updates? did you confirm it was DNA?
Good question
It is DNA
@@МиленаКамчева Лаборантка? :)
You say looking at lood has no diagnostic value ! How is it that you see sichel cell ? Or how is it when you see an infection . Or let me go further how can you see if blood is infected ?
To check for infections, you do a PCR or an antibody test. There are only very few infectious diseases that can be diagnosed with microscopy. Malaria being one of them. Microscopy is simply not specific enough. If you have an infection in a wound, then you can of course isolate and see the bacteria, but then you already know that you have an infection....
i've never done more than school microscopy, but i wondered if just putting like an piece of plant or insect on a glass was enough to see inside and since you said something with dish soap, that breaks the structure and makes the cells inside visible, is this the way to make the inside visible? or is the inside visible anyway? i've read thing about microscopic knife too...
I tried it and it worked!...Thanks!
The wood also good in case any of the DNA happens to be vampiric.
First of all how do u know this steps i mean did u learn it somewhere or read it in some text book ? How can i make sure that what u did now is real science ? Thank u
Can you make a video of mushroom cells under a microscope?
Wow how smart... Is the rounds things cells
The laugh after “wood is good” XD i actually died
Hi, this is really interesting. What if we try and see bacteria in that solution? Would this be possible?
Awesome experiment sir 🙂.Thank you for sharing.
Great day for me to see DNA live. Thank you.
Amazing as always. Thank you for all you do. This is my favorite video I have seen.
Good one! Great topic. What kind of microscope are you fundraising for? I want to read the specs. :)
It's an Olympus BX53 with Planfluorite objectives (4x 10x 20x 40x 100x oil) and Differential Interference Contrast (DIC), which is expensive but produces very nice images. You need a separate prism for each objective and they need to be fit in into a condenser with a rotating turret. I obtained several offers, all similar in the price range, some even more expensive.
@@Microbehunter OoO an Olympus! I hope to be able to donate too!
Can we also use forceps to lift the DNA ?
I’m a cosmetology teacher and I have thoroughly enjoyed this. I want to find a microscope with proper lenses and magnification to view hair at its cellular level. I’d also like to be able to view things like hair color’s molecular structure. Any ideas on which strengths I may need?
There are many options. You might want to have a look at my other channel for help: www.youtube.com/@microbehuntermicroscopy
DNA reacts with diphenylamine based on the conversion of deoxyribose sugar in the DNA molecule (not in RNA), so when hydrolyzed at high temperature in an acidic environment to form hydroxylevulinyl aldehyde which reacts with diphenylamine to form a blue complex sustainable construction. So you can do this extra step to identify colored DNA when observed under a microscope and see :)
How it possible to see a small molecule to under the compound microscope because the resolution is 0.3 micrometer and magnification power is 1500x while the size of DNA molecules is 0.6 manometer.
Is it in compound microscope???
How do you know it is DNA and not bits of salt, washing up liquid, old food bits, saliva or water etc?
Bro it's awesome and you are a good content creator
It has a beads on string structure so probably is chromatin material.
yeah i agree
You are an amazing person,
This is my first video of your
Not only is the video cool, but the micrograph website is really cool!
Could this be done with a cheap educational microscope? Like a normal 1000x compound scope?
Keep in mind I know NOTHING about chemistry and little about DNA, but could this be mucus or something? It certainly acts like it, but I dont know.
The microscope must be good and I also used phase contrast. If the microscope has a proper condenser, then maybe yes.
I've done too at my school! Although the result was different from yours , but it made me really excited😍
What is magnification Sir ? Thank you
Nice work!! What happens if you rhythmically press on the cover slip for a while? Does that break up the clumps of DNA?
So what is the Jelly like substance surrounding these strands of DNA?
And how much it costs?
Oliver, where are you located?
Dishwashing solution. A non ironic surficant dissolves away the fatty acid in the cell membrane and lets the contents spill out. Great for disinfecting also
What microscope did You use that must be very powerful Is it by olympus?!
I am using an Olympus CH-40 and an Olympus CHA with phase contrast. Other microscopes should also work.
I wonder if you can look at them whilst they are in liquid...
can you see the sars2 pathogen?
I love the moment you pull your saliva from the measuring cup and call it a clump of DNA :D
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Followup Video - Better DNA extraction: th-cam.com/video/3qvm3w2zwgA/w-d-xo.html
Which stain is better when it comes to plant tissue
Which microscope🔬🔬 you use to se dna normal or elecron microscope
Watch this: th-cam.com/video/wi8kpsoxwBE/w-d-xo.html
What about messenger RNA? Such as a corona virus? Just curious how they come up with the corona image..
Can u do a study on the plasma membrane f human rbc
could the dna be with a smalllll drop of water or mixed in with a very tiny amount of water
Where did you get that test tube?
Thank you so much for doing this video. I found it exceptionally engrossing. I appreciate learning how scientists make the "DNA" strands they can take out of the tube. I've always wondered about that. Bravo!! 👏
can you use an alcohol based alochol instead
Thank you so much sir for this great work.I was really excited to see DNA . Love from INDIA🇮🇳 ❤️🇮🇳
You are terrific! I love your presentation and the information you bring us. Two thumbs up!👍 👍
Thank you for creating this image database!
Sir which microscope do u use
Can you suggest a home available stain that would be more specific to DNA that would be fairly non-toxic?
Since DNA is acidic, maybe there could be a basic dye (I am thinking that Malachite Green available in the pet section of stores for Fish Ich, but know it is highly toxic).
Aloha from Big Island!
-Sid
Sir plz show some solid elements powder or stuff like that in a microscope..
Which microscope are you getting?
What is used to actually see DNA strands?
electron microscope: but barely.
Please make a video on how to match two dna
Hi which is the best electron microscope?
I really enjoy seeing microbes. I am glad to have found your channel. Thank you!
Would be good to see magnification rate on website
I still wonder if my science teachers were this amazing ..I would have choosen science for rest of my life...
Though with no regrets ... Being a commerce graduate...I still carry my interest for science experiments. 😀
Not too late my friend, sit down, make plans and go for it, live while you are alive.
But i thought RBC don't have a nucleus as they have to store hemoglobin
Sir... Which microscope is best for student
Sir how can we extract dna in intact form ?
It has to be done in a lab. However it is not intact. Once you remove the histones (proteins keeping DNA integrity) when you try to purify DNA, you end up with broken pieces of denatured DNA. However for lab purposes, even broken DNA is useful.
Which tupe of microscope you use for see the DNA....??????
This was a phase contrast microscope. But these are not individual DNA strands, but most likely several bundled together. Individual DNA strands can not be seen under the microscope as they are too thin.
How much magnification do you need to see cells
You can see large cells (onion) already with 40x total magnification. Red blood cells can be seen with 100x and more.
What dish wash solution is it?
🇮🇳Nice video,Excellent sharing but how could we test between 2personal that their DNA are similar or not..
These may be are the microtubules or Intermediate filaments also'.
The sound when he spit out salty water was satisfying 🙏👍👍😂😂
😁 I really like the Microworld Archive idea! Which microscope and camera are you aiming for?
But how do you know whether your DNA or bacterial and other microbes DNA?
that would be difficult to know with the steps he took. He used saliva; there are bacterial DNA mixed as well for sure. The only way to know if it comes from bacteria or him, is to amplify it and run the DNA code. However, his video is more about how to visualize DNA in a light microscope. The DNA is going to be mixed and from different sources for sure.
Great video again
Loved it!!
Does that make dna grow??