Osmotic Fragility Test - Dacie's and Sanford method

In this video, we will be discussing the Osmotic Fragility Test, both Sanford and Dacie's Methods, interpreting results, and clinical significance.
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[@irfanbash]

Nicely presented topic. Well done

[@jaspersiron1041]

I love this video. I hope you still continue to make this kind of content coz this really simplifies the topic despite its complexity. keep up the good content. Salamat!!!!

[@patriciadones6271]

HI! What are the advantages and disadvantages of Sanford and Dacie's method?

[@pratiwiratih5761]

Thankyou

[@kirstenkendall5716]

Why would you subtract the 0.85% NaCl solution from the absorbance values if you are using it as the blank? Could you not just zero out the spectrophotometer with the 0.85% blank? If so, would it not just be the absorbance of the sample tube divided by the absorbance of the negative control tube, multiplied by 100?

[@ClinicalLabScienceVideos]

Hi. The blank being used here is plain 0.85% NaCl without blood. Meanwhile for the equation, the absorbance being deducted is from the supernatant of the 0.85% NaCl tube with blood. Please check out this video for the demonstration: th-cam.com/video/NWn3gpXnS1k/w-d-xo.html

[@kirstenkendall5716]

@@ClinicalLabScienceVideos Ok, I'm sorry. I missed that during my initial watch. Thanks for clarifying.

[@QiqaLukhanji_BhudaQ]

Is it the release of hemoglobin that causes colour differences

[@QiqaLukhanji_BhudaQ]

?

[@ClinicalLabScienceVideos]

Yes it is.