Bioprocessing Part 1: Fermentation
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- เผยแพร่เมื่อ 2 พ.ค. 2024
- This video describes the role of the fermentation process in the creation of biological products and illustrates commercial-scale fermentation at a cellular level. Included are descriptions of types of fermentation (intracellular, extracellular), types of cells (aerobic, anaerobic), and cell nutrition (media components).
The program follows the production of a batch of Green Fluorescent Protein (GFP) from preparation through harvesting, including growth of seed stock, scaling up, and life cycle growth pattern phases (lag, exponential/log, stationary, death). Featured throughout is the monitoring of various production process parameters, including time, temperature, pressure, pH, agitation, etc.
For free eLearning resources, check out www.ncbionetwork.org/education....
I do this for living. If you want to work in this industry, I would recommend joining upstream first. Upstream is less hectic. Understand the science behind the processes and then transition into downstream. Downstream is more laborious. Thank you for such a great video.
Thanks for the advice.
You very right.
Having worked in both upstream and downstream development and manufacturing, I think each has its attractions and challenges. Upstream is more biology focused, handling microorganisms or different cell lines to make the desired product at the yield and quality needed. Downstream is more biochemistry focused, where you separate the desired product from the other impurities based on their physicochemical properties. Upstream can be a bit monotonous if you are using the same type of organisms, somewhat challenging as it is harder to understand/control due to the biological element. You are also bound by the growth scedule, so once you start an experiment, you have to regularly monitor, take samples, which may require odd hours, weekends, etc. But it is also exciting as you can engineer your organism to make the most product, and also can see the interplay between biology and physical parameters. Downstream has more operations, so there is more variety. The physicochemical nature does allow use of more fundamental molecular properties to understand/control performance. There can be a little less time dependence, especially if the molecule is not too unstable.
Dear Mister rabbani, im a newbie at this work as biotechnolog, It‘s very hard and hectic at the USP.(For me) im trying my best every Day to learn and keep on the Ball rolling, but then there is the moment, i get to do a task where i m alone to do it by myself the work then my heart Starts to pump faster, Start to breath heavier there is the fear that somethink can go wrong, i cant handle this ,sometimes im really thinking on to leave the work.. but i keep going. Can you give me some advise?
@@SMFighter91 Are you working at a cGMP facility? If yes, then you should always be working with a partner. If not, then you should only be working solo after you are completely trained. And about fear, throw that out of the window.
This video landed me a job in very big biotech company, god bless u guys
Shantanu Kukreja ... congrats brother
Is there any vacancy for QC or Production there?
congrats!!
Genentech or gtfo
haha same here
Nice sir..which company?'
Good to learn about bioprocessing, as in coming years this can become the state of the art, depending on its broader spectrum of application...Thank you HRDC-GU
Really very useful video!!! thank you for explaining the entire process stepwise!!!!... i can get a better view on this process
Thank you for this video! Very informative, great work!
Informative session on "Bioprocessing Part 1: Fermentation".
Fantastic supplement for my revision, thanks
Excellent video really helpful, much appreciated
Well-done! Thank you!
Loved the detailed video. Thanks so much!
This video are very informative about biotech levels
seriously finally I could understand my latest lec Thaannksssssssssssss a lot
Thanks for the video, it helped me a lot. I was wondering what specific model of Sartorius bioreactor is the one showed on this video. Thanks!
Hi, do i have your permission to use the video for a Microbial Biotechnology course in my university? Thanks alot!
It is one of the best explanation I ever watch, Thank you sooo much.
Glad it was helpful!
Thank you for explaining the entire process stepwise
Thank you for the video and the information.
Beautiful fermentation technology
Thank you! That helped me a lot
Thankq...its very helpful for my studies
Excellent !!
Thanks for the video. Can I download your video for offline educational purposes?
Excellent video about fermentation technology
Thanks for the bioprocessing lecture video
Are these videos available for corporate training? If so, where can we get licensing details and pricing? Tnx!
Very informative article very good video
Very interesting topic, Thank you sir
Thanks for wonderful explanation and the video!
Glad it was helpful!
Great video! Thanks 😊
Nice explanatory video on fermentation technology. Thank you
You are most welcome
Excellent,
I plan to use in my course for Industrial Microbiology. how could I do it without violate your c/p rights??
+Jesus “ChagoKOKI” Santiago You can use the video link for free for educational purposes. Thanks for asking!
Ok so I'm here for learning howit actually works :) A 12th Grade student though!😊 v nicely explained❤️
Dear authors, thank you very much for your work. Can I use this video in my lectures on biotechnology?
I watched this with my students on youtube.
Informative session
Amazing! Using to prepare for interview
Good Informative video...
Thanks for the video
I am a Biochemist, utilising the biological metabolome of different strains of microorganisms and utilising the evolutionary and genetic capabilities of such organisms can enable humanity to produce any component and henceforth provide solutions to any problem. You could even make a bacterium that can produce or up-regulate a substance that absorbs CO2 to combat climate change. The possibilities are endless I always like to re-visit the basics to solidify my understanding
fantastic explanation, thanks for sharing!
Very nicely explained.
Nicely explained thank you
Does the SIP process happen with components inside? Shouldnt the empty vessel be sterilized. Am I missing something?
glucose analyzer you can use Siemantec from China M-100 biosensor analyzer
😊Thanks Rabbani, I appreciate the advise you gave. I am about to join a company that has downstream and upstream department.
very nice.....thank you...
Very informative video
Great 👍🏻
Excellent
Really very useful video
3:21 which process is shown hier? Thanks in advance.
well done
Please make this video available for offline purpose...
Very good information video
It was very informative
thanks and thanks and veryyyyyyyyyyyyyyyyyyyyyy thanks!
WOW!!! Fascinating info, tho the Closed Captioning could really use some punctuation. Periods, commas, and Capitals would greatly ease the information understanding. Thanks for all the hard work.
Thank You
excelent video
Very informative
well, i'm just curious. why use glucose, why not sucrose, isn't sucrose cheaper?
Is there any way to extend the E colis life span? can you add a bolus of nutrients it needs to keep it going?
You would also have to contend with buildup of waste products that inhibit further growth. I don't believe there is any facile way to remove them from the broth (perhaps there are filtration methods, I don't know), and even a small 1:10 dilution would be impractical because this would require around 180 L of fresh media to the existing 20 L.
bom vídeo.
After MSc biotech can I get job in mumbai or not ?
Thanks
Great thanks a lot
Interesting procedure , via Linux I can optimize this.
This video is our course in microbiology industrial
Thnx u v m
Hi. Excuse me, I had a question: I want to produce 70% alcohol for disinfection by combining sugar and baking yeast and distilled water. What should be the temperature of the fermentation liquid in the barrel during the fermentation stage? What should be the ambient temperature? I am a member of your channel and I watch your clips. Thank you very much and good luck.🙏🌹🌷⚘💙💜❤👍
For microorganisms, a 70% concentration of ethanol cannot be reached. Ethanol inhibits growth as soon as the concentration rises to about 10-15%. After that, you have to distil it. Only wine and beer can be produced without distillation.
@@halihammer Yes that's right. I also meant 70% distillation. Thank you very much for your help.🙏👍🌹🤗
Nicely explained but lengthy
Guys, does anyone know why antibiotics are added to the mixture? I mean e. coli is a bacterium ... and antibiotics are against these kind of cells. But these cells are mandatory to produce the product, in this case the GFP
I would appreciate any logical answer
at 9:45, why the growth chart is like that? why is it started with OD=0. During 0 hour inoculum should be added so the value could not be zero.
If you look closely, OD at 0min is higher than zero. Granted the inoculum will be diluted manyfold when introduced into the tank!
Nice video
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Fermenting: have been using small ultrasonic cleaners for making Aging alcohol Cannabis edibles, Tinctures and Coloidal Silver.I had the Idea to try it on my Fermentation of grain mash. My hopes were to make the mash into smaller particles and increasing surface area. I hoped to increase Bioavailability for the yeast. The 6 gal. plastic bucket I used for it kept blowing its top off as the bubbler was unable to keep up with the new rate of fermentation. I had to resort to taping the lid down with duct tape, Watching the bucket bulge from unreleased pressure and hoping it did not explode. !!WARNING DO NOT USE A GLASS VESSEL FOR FERMENTING IF YOU SHOULD TRY THIS METHOD!! I had hoped to show the increased speed of fermentation in this video. I made two mistakes. I used some pretty old yeast and I did not malt the grain first.
Still the control had not even started before the ultrasounde grain had filled its bag full. I believe if I had used a malted grain and newer Yeast the results would have been more dramatic for you to see. I am very new to brewing, only three batches with using ultrasound to decrease particle size the third batch. I did try a sugar wash 4th batch using the ultrasound but saw no changes in the speed of fermentation. . I am hoping someone with a better set up and greater brewing knowledge can experiment with this and show the actual rate of increase. (I believe it to be about 300% or better)
This could have a major significance in such a large market as alcohol and fuel.A factor to consider is stirring the grai water mixture in the ultrasound as a heavy layer of grain at the bottom may reduce effectiveness. I used a 60 watt, 40khz ultrasound unit with just one transducer on this video . On the earlier bucket run I used a home made unit with two 40 khz transducers. Finding the optimal range of transducers, frequency , volume, stir rate and length of time I leave to future brewers with more capital and time to find
th-cam.com/video/b_mdvsEF3vo/w-d-xo.html
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เเปลให้ฟังหน่อยคับ
💙
Wonderful work! Wanna do some cowork project. How can I contact the team?
Tommarow am going for an interview in this same industry... 😇😇😇😍
What asked from you ..Could share some information regard USP and DSP in industry
Tomorrow I have interview for USP and DSP so please guide
nice
Very easy
What sizes are the Bioreactor and Broth tank used in this video?
1-2 hL, approx
na na na epico el video igual capaz que le falto algun micro nutriente distinto no tipo para producir mas que se yo
Basic information to start ...
Good
how is the human cells obtained and from where?
What human cells???
Probably HeLa Cells. Read "The Immortal Life of Henrietta Lacks"
0Ka
ராமாயணம்
Mammalian cells
การทำยาเครื่องจักรนำสมัยมากคะ
อาจเป็นได้ในบางครั้ง เป็นที่นิยมมากในขณะนี้
good video! but what the heck. that yeast powder is flying everywhere lol.
Beautiful love u.
I am watching this video 8 years later
This randomly appeared in my recommended lol
Imma study biotechnology
No normal fermentation.
hilarious - i'd hate to taste his home brew
acmullane home brew is the whole reason I started watching this vid😂😂😂😂
Corrosion Control #POW
Pk elle nest pas en français 😦😦😦
Elle est maintenant.
@@NCBioNetwork cool 😍
Who is here bc of professor Bi? :)
-Guy chewing gum in the fermentation room.
-No mention of cleaning fermenter before use.
-Syringe and tubes weren't labeled.
-No syringe filter on syringe addition.
-Adding YE and soytone at the fermenter site creates a dust cloud of contamination.
-Otherwise a nice video.
Tm logo ke waja sy hameri teacher ny humy peryshan keya hoya ha
Zaleel hogye hain hum log tum logo ke waja sy kya zaroort the English ma video banany ke
How annoying is his voice