the RBCs count you mentioned was counted on the 5 small squares inside the middle square. Isn't the area calculation should be 0.2mm x 5 (squares) x 01 (depth)? Take a look at 23:17. I'm confused.
Hi. Thanks for this comment. Each small square in the middle square has an area of 0.04 sq. mm (see 9:36). Since we counted cells in 5 small squares, the total area counted is 0.2 sq. mm (0.04 sq. mm x 5 squares = 0.2 sq. mm).
In RBC COUNT, WHAT DO YOU MEAN BY TOP AND BOTTOM CHAMBER while you are saying we count 5 intermediate chambers? And why are you doing average of them with 2?
There are two counting chambers in the hemocytometer. They are separated by a channel in the middle of the hemocytometer. You need to count the cells in both. A 10% difference in the counts between the two chambers is indicative of faulty charging. In these cases, you need to repeat the charging process.
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Kinda didn't get the part in rbc count where you got the area ? The one with 0.02 and 0.04 as you said
the area of the each different intermediate square in the center square is 0.04 square millimeters.
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Why dilution factor of 1: 20 is 20 but not 21?
the RBCs count you mentioned was counted on the 5 small squares inside the middle square. Isn't the area calculation should be 0.2mm x 5 (squares) x 01 (depth)? Take a look at 23:17. I'm confused.
Hi. Thanks for this comment. Each small square in the middle square has an area of 0.04 sq. mm (see 9:36). Since we counted cells in 5 small squares, the total area counted is 0.2 sq. mm (0.04 sq. mm x 5 squares = 0.2 sq. mm).
Thanks for the clarification!
In RBC COUNT, WHAT DO YOU MEAN BY TOP AND BOTTOM CHAMBER while you are saying we count 5 intermediate chambers? And why are you doing average of them with 2?
There are two counting chambers in the hemocytometer. They are separated by a channel in the middle of the hemocytometer. You need to count the cells in both. A 10% difference in the counts between the two chambers is indicative of faulty charging. In these cases, you need to repeat the charging process.
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