I've watched 3 other videos on this, plus my professor's lecture, and read the textbook... I was on the verge of crying because I felt so dumb for not understanding it and only your video made something click in my brain!!! Thank you so much really, you have a gift for teaching science
The most amazing lecturer EVER !!!! I cant thank you enough, i bet i will have watched every single one of ur videos by the end of my first biomed year ;)
Good sir, I just want to let you know that your explicit lecture didn't just save me, but also many of my fellow classmates who rely largely on my detailed notes to understand this concept. I cannot express enough of how appreciated I am.
I'm a Premed student and can't understand genetics anywhere more than from your channel. You are amazing! I wish you make more videos on higher-level, enhanced and new genetic concepts for students of master of genetic counseling and geneticists.
how many cant wait to finish school, get a job, come back and than this guy. now in my fourth year Bsc Biotechnology and AK Lectures team have been making my life easier
Even though 2 years have gone by since you published this I feel compelled to say THANK YOU! I stumbled on your video after looking at another 6 much shorter videos which left more questions than answers. While it firstly seems you repeat concepts it is actually VERY useful to follow all of the reasoning. My Genetics was studied back in the 80's (I am a medical doctor) and your video is just what is needed for a good intelligent refresher to our current knowledge.
Genuinely thank you SO much for ur videos, don't know how manage to make these complicated concepts look soooo easy but u just do. I remember them for ages after studying them from u
Dude.. Your lecture just gave me that chill of discovering how awesome science is.. It hasn't happened to me for a long time ever since I took this damn MCB course.. Thank you so much..
Hi, Andrey! I am doing Molecular Pathology subject at uni now. It is very complicated..and your short lectures help a lot! Thank you so much for that! Big hug!!! :)
Thanx to AK class for providing such clear concepts... I was finding for a splendid bio channel on youtube and i hope i've got u... Thank u sooo much... :-)
Sir many many thnak u which i can't explain here.❤❤ This video helped me a lot..after Professor & many video lecture, finally i understood Sanger method by ur lecture....... 🥰🥰🥰 Tnq tnq tnq tnq tnq tnq🥰🥰🥰🥰🥰
At 11:30 you talk about 'SDS-PAGE', but isn't that type of gel electrophoresis only used with proteins? (The SDS is not needed, since DNA is already charged.)
AK, DNA is not susceptible to alkaline hydrolysis, as in sodium hydroxide. It's RNA that's susceptible to sodium hydroxide. However, DNA with HCL will lead to a selective removal of its purine bases by cleavage of the purine glycoside bonds.
How can you determine the sequence in the beginning of the strand to make the primer? 10:41 you said there is a possibility that it doesnt use the ddatp in strand 2, and instead is used in strand 3 Also, if only strand 1 and 3 were to be produced, how would you determine the skipped A in strand 2?
Opus 0 Dentistry hopefully but I'm taking a gap year this year. How did UKCAT go because I'm doing that after exams and I'm scared lol I don't Where to start.
Elizabeth Roberts Yeah UKCAT sucks ass, such an meaning less test!! I only got like 2 weeks of practice using medify (best resource for UKCAT! Screw the books this is the best by far) All in all I got a 630 -which is average - I was bummed out about that, but things worked out with a UoB med offer at the end
Grat video, well explained, thanks. My question is how can I have the primer complementary if I have to find the DNA sequence? Do we use database or other? If we use database, how did the biologists when they didn't have any information? Sorry for my english :)
+random we would cut the DNA with a restriction endo nuclease enzyme and which has a property to cut DNA at specific sites.... like BAMH1 , HIND1, etc....
We can just add a segment of DNA that we know it sequence by clonnage method while we may produce a primers are complementary to that sequence . So we can sequencing the unknown DNA sequence. Sorry for my English 😊
You said that the segment either has a chance to bond with ddNTP or dNTP; what if it bonded with dNTP for all of the possibilities; wouldn't it express a 4th fragment AKA the entire DNA segment?
I've watched 3 other videos on this, plus my professor's lecture, and read the textbook... I was on the verge of crying because I felt so dumb for not understanding it and only your video made something click in my brain!!! Thank you so much really, you have a gift for teaching science
Of the 3 videos I've watched on this, only AK managed to clearly explain the concept. Thumbs up.
When I am done with medical school, you're getting a good donation!
So, are you done yet?
ME TOO
AK lectures are absolute life-savers!!
@Alyssa Colbert good luck!
My thoughts exactly. Same goes for Nancy Pi
I always come back to this video before my Genetics tests and exams - it's a faster way to learn it then reading through piles of notes. Thanks!
The most amazing lecturer EVER !!!! I cant thank you enough, i bet i will have watched every single one of ur videos by the end of my first biomed year ;)
Good sir, I just want to let you know that your explicit lecture didn't just save me, but also many of my fellow classmates who rely largely on my detailed notes to understand this concept. I cannot express enough of how appreciated I am.
I have been following you, and all I can say is you are a genius. You teach with clarity and simplicity
I've watched many Sanger sequencing videos. This one is my final stop. Thanks Sir!
these are by far the best videos for bio/ med students on youtube - we can't thank you enough!!!!
Professional, Clear, Concise, and Thorough. Thanks for the great video!
I'm a Premed student and can't understand genetics anywhere more than from your channel. You are amazing! I wish you make more videos on higher-level, enhanced and new genetic concepts for students of master of genetic counseling and geneticists.
One like is not enough for this video. This is hands down the best video on this topic!!
This was incredibly well-laid out and helpful! Thank you for going through a complete example from start to finish.
This is the only video on you tube which really explain the sequencing. AK you are too good.
how many cant wait to finish school, get a job, come back and than this guy. now in my fourth year Bsc Biotechnology and AK Lectures team have been making my life easier
Can't explain how helpful these videos have been! Thank you, thank you so much!!
This is the best one out of 4 videos I watched. I was so lost. Thank you so much G.
The type of a teacher I wish I had for the rest of my studies... thank you very much!
The best explanation on youtube. Thanks!!!!
+Dill Mart Thanks!
Thanks 🙏
YOU!!!!! You are the one to finally get this into my head. Thank you so much for this video, you're a legend.
Good work! Watched the channel during undergrad, then masters and here I am while doing PhD!
Thank you for being a long-time viewer! Best of luck with your PhD!
Even though 2 years have gone by since you published this I feel compelled to say THANK YOU! I stumbled on your video after looking at another 6 much shorter videos which left more questions than answers. While it firstly seems you repeat concepts it is actually VERY useful to follow all of the reasoning. My Genetics was studied back in the 80's (I am a medical doctor) and your video is just what is needed for a good intelligent refresher to our current knowledge.
I just signed in to like this video because this video deserves to be liked and commented.
Genuinely thank you SO much for ur videos, don't know how manage to make these complicated concepts look soooo easy but u just do. I remember them for ages after studying them from u
You are honestly the most amazing teacher. Thank you, since it's rare that I can follow perfectly.
AK lectures are fantastic.Each time I'm in problem you are the one who solve it.
Thank god for your simplicity! You just made my presentation for Sanger vs next gen extremely easier! Thank youuu! Keep it up!
God Bless you man. It's hard not to learn something new watching your lecture. Your channel will be my winter break!
That was so clear. The flow in your explanations worked so well i could easily follow every word you saying. Thanks a lot.
wow i thought this was way more complicated than it looked. Thanks for making it simple
This is the most understandable video I have ever seen. Thank you, Andrey!!!
Dude.. Your lecture just gave me that chill of discovering how awesome science is.. It hasn't happened to me for a long time ever since I took this damn MCB course.. Thank you so much..
Also I played this video at 2.0 speed and max volume, which kept me concentrated af lol
Oh thats pretty amazing Tommy; that chill is hard to come by, glad this content gave you that! Keep at it and best of luck in your MCB course!
+AK LECTURES (Andrey K) Thank you!
Honestly you always save my life i can't tell you how much i'm thankful for all your lectures
I'm binging on your channel in preparation for my bio-chem final tomorrow morning. Thanks!
i am such a fan of yours ... your way of teaching is beyond the top... great job thank you
Thanks Anna! glad you enjoy it!
so easy, when explicated by dude like you. peace from Morocco
Hi, Andrey! I am doing Molecular Pathology subject at uni now. It is very complicated..and your short lectures help a lot! Thank you so much for that! Big hug!!! :)
Thanks Sveta! Good luck with the course! study hard :)
You saved my life. I have been struggling with this
You are a great help for my BSc in Sport Sports Science.
it can't be better thanks a million I was kinda lost, your lecturer makes it clear to me
In the video you seem to have written ddNTPS as nucleoside instead of nucleotide , an honest mistake perhaps . The video is great btw!
you are the best thing that has happened to biology students ok
You are great at teaching! Please never stop making such videos! Awesome style! :)
lecture kha naday.
The best explanation of sanger sequencing!
The best video about this topic
This video was awesome! You saved my grade.
Sir, please upload some videos on the Next Generation sequencing , that would really be a great help... Your lectures are as always AWESOME.
Thanx to AK class for providing such clear concepts... I was finding for a splendid bio channel on youtube and i hope i've got u... Thank u sooo much... :-)
OH MY !! THANK YOU SO MUCH !! I FINALLY GET IT, it's so clear
Sir many many thnak u which i can't explain here.❤❤
This video helped me a lot..after Professor & many video lecture, finally i understood Sanger method by ur lecture....... 🥰🥰🥰
Tnq tnq tnq tnq tnq tnq🥰🥰🥰🥰🥰
You are too good to be a professor
I like the way you teach, great job brother!
Amazing explanation, I also came away thinking that Sanger is a genius
At 11:30 you talk about 'SDS-PAGE', but isn't that type of gel electrophoresis only used with proteins? (The SDS is not needed, since DNA is already charged.)
Love you man.. You're a gentleman and a scholar.
Just perfect as always, AK!
thank you so much, so comprehensive. I wish you the best on your study and career
thanks.... it was really helpful.... I had cleared my issues about this topic with this vedio.... thank you for the good work
Thanks a lot !
Huge respect from india!
You make me pass the exam..Thank you..
Wowww! You are such a excellent teacher!
Wonderful,I had doubt about this from the beginning!😢you made it so clear👍👌👌👌😊☺️
Fantastic explanation, very clear and easy to follow.
I love your videos so much. I understood my subjects very well during biomed 1st year
He is not easy yo follow. But really outstanding if you want to learn.
Your videos are so helpful! Thank you
AK, DNA is not susceptible to alkaline hydrolysis, as in sodium hydroxide. It's RNA that's susceptible to sodium hydroxide.
However, DNA with HCL will lead to a selective removal of its purine bases by cleavage of the purine glycoside bonds.
Excellent video, I understand this now.
But at 7:50 I think you mean to say "anneal" rather than "hybridize".
same thing
@@damilolakadiri1857 Yes, you're right. Thanks
how do you determine what sequence is on the original strand to build the complementary RNA primer sequence?
Exactly what I want the know
@@sputnikaguya the same also... i dunno how did we make primer comolementary to the DNA template and we don't know the DNA sequence !!??
Great question! I'd like to see a video on that as well
Yep bro i also got the doubt in that
Few known nucleotides are added to the original strand so as to make the primer.ok
you are very good at explaining . Thank you so much , this really helped ALOT
best explanation ever
keep on good work
I am very happy and now I like your lictures too much
You make it so simple! Thank you!!
I am so grateful for this video.
the only presentation that made sense
5:08 "we have to know what the complementary sequence is"
- how is that found out? How do you learn that?
Great video! Very clean job.
Thank you!
Greetings from Italy!
You son are going to get me a +520 on my MCAT!!!
How did it go?
Your lectures are awesome. Thanks
Thank you for enlightening me :)
thanks to your video tutorial I'm going to pass my molecular bioinformatics exam :)
Really amazing , I can get the information and concept clarification easy wow really thumps upppppppp
My favourite youtube video
How can you determine the sequence in the beginning of the strand to make the primer?
10:41 you said there is a possibility that it doesnt use the ddatp in strand 2, and instead is used in strand 3
Also, if only strand 1 and 3 were to be produced, how would you determine the skipped A in strand 2?
Superb video. Thank you so much for creating this content.
this is crazy because I'm watching this video and I'm only doing a levels but I understand pretty much everything!
yup same here, not too difficult. Good luck on you're exams
btw are looking to go to med school as well?
Opus 0 Dentistry hopefully but I'm taking a gap year this year. How did UKCAT go because I'm doing that after exams and I'm scared lol I don't Where to start.
Elizabeth Roberts
Yeah UKCAT sucks ass, such an meaning less test!! I only got like 2 weeks of practice using medify (best resource for UKCAT! Screw the books this is the best by far)
All in all I got a 630 -which is average - I was bummed out about that, but things worked out with a UoB med offer at the end
Perfect, clear and concise. Thanks!
Congratulation man, very clear!!!!
You are a life saver! thank you so much
this guy is perfect !!!!!!!!!!!!!
so the concentration of tryptophan itself determines the rate at which more tryptophan will be produced - so interesting!
Grat video, well explained, thanks.
My question is how can I have the primer complementary if I have to find the DNA sequence? Do we use database or other?
If we use database, how did the biologists when they didn't have any information?
Sorry for my english :)
I wonder this too, how do i we choose a complementary primer when the whole purpose of this is to find the DNA sequence which we do not know.
+random we would cut the DNA with a restriction endo nuclease enzyme and which has a property to cut DNA at specific sites.... like BAMH1 , HIND1, etc....
May I ask about the database are ???
We can just add a segment of DNA that we know it sequence by clonnage method while we may produce a primers are complementary to that sequence . So we can sequencing the unknown DNA sequence. Sorry for my English 😊
better than my lecturer ^^
Best explanation!!
I love this man. God bless you!
I can't thank you enough for such great videos
Really appreciate it brother! keep up the good work
you are amazing teacher
4:00 "we isolate it and place it into a beaker"
- how is that done?
Marvelous explanation .. Thank you ..
You said that the segment either has a chance to bond with ddNTP or dNTP; what if it bonded with dNTP for all of the possibilities; wouldn't it express a 4th fragment AKA the entire DNA segment?
Yeah good question plz leave an answer
yes, you are right, but have not put too much normal nucletides( dNTP) To happen that, we have put only 4 dNTP, after four, it will go for ddNTP.