Next Generation Sequencing 4: Checking Nucleic Acids with an Agilent BioAnalyzer - Eric Chow (UCSF)

www.ibiology.org/techniques/n...
Next generation sequencing allows DNA samples to be sequenced quickly and affordably. Learn how next gen sequencing works and get tips on preparing and running your samples.
In the past decade there has been an amazing change in the efficiency of DNA sequencing. Using traditional Sanger sequencing, the human genome project took 20 years and cost $3 billion. Current next generation sequencing methods allow a human genome to be sequenced for $1000, in 48 hours! In this talk, Eric Chow explains the chemistry behind next generation sequencing, and describes how the next gen sequencers detect and display results. The most commonly used Illumina sequencers are image based and detect the addition of fluorescently labelled nucleotides. Chow also describes two different next generation sequencing technologies which provide benefits such as much longer reads but with downsides such as higher error rates. Chow finishes the talk with some insights into medical applications of next gen sequencing such as much less invasive prenatal testing or cancer detection.
In two short how-to videos, Chow gives advice on purifying DNA samples using magnetic beads and on determining the quality of your nucleic acid sample using an Agilent Bioanalyzer.
Speaker Biography:
Eric Chow is an assistant professor in the Department of Biochemistry and Biophysics and the Director of the Center for Advanced Technology (CAT) at the University of California, San Francisco. The CAT provides resources for UCSF labs wishing to use next generation sequencing techniques and Chow’s research program strives to develop new applications for NGS in pathogen diagnostics. Chow received his BA in molecular biology from the University of California, Berkeley and his PhD in biochemistry from UCSF.

[@farrahphillips8461]

This video has been very helpful for me. Thank you for producing this well organized and thorough tutorial!

[@DaniloMicali]

That's a great and very helpful video! Congrats!

[@Kill4Jill]

Great Video Man! Keep up the good work !

[@Genenat]

Fantastic video! Thank you so much.

[@AqleemAbbas]

Dr. Eric Chou Explanations are very nice.

[@orxanibayev340]

Fantastic material, many thanks!

[@vkuri2060]

Excellent video mate.

[@Fattiapples]

Awesome video! Would love more.

[@spindlesthesquid]

amazing video, so helpful! thank you!

[@narminhamidli840]

such a great video!!

[@Shaq0112]

Very well presented. thank you very much

[@konibei8786]

Love it, thanks!

[@aidajameei1552]

Thank you s much. Hope you talk about RNA RIN value as well. Thanks

[@tatyanazhebentyayeva1807]

Very good presentation! Thank you! The only missing detail for beginners is proper storage of gel and other chemicals after opening kit.

[@sm-kz4qt]

Pages 12-13
www.agilent.com/cs/library/usermanuals/Public/G2938-90321_SensitivityDNA_KG_EN.pdf

[@bhangrafan4480]

Excellent exposition.

[@ylidene6222]

Thank you! It helped me a lot

[@deepakkumarverma4659]

Excellent sir thanks

[@raquelrodriguesperes6948]

Great, thanks!

[@Migshaw]

AWESOME!

[@johnnykim233]

Very helpul thank you

[@bluedrag00n]

This rocks!

[@readme373]

excellent

[@hskim352]

非常感谢

[@lilianjose9154]

Thank you very much for this video. It helps a lot! However, may I check on the reagents (especially all related to the dye) because as what I read in the Agilent High Sensitivity DNA Kit Guide, I understand that the reagents are light-sensitive. But in this video, it does not seem that way. Can anyone help in this matter? Thank you in advance.

[@dominijerzboi]

Hi Lilian, I use this instrument daily for my job in cancer research and in our lab we always store the reagents in a dark box due to it being light sensitive especially the dye that gets mixed with the gel as well as the other reagents.

[@desireeheringlehner7842]

I use the RNA Pico Kit but I´m wondering about your gel preparation. In my instructions it says to centrifuge the gel (spin filter) and add the dye AFTER (centrifuge again).

[@potanii193]

When you said you are adding gel/dye mix to other 3 gel wells but you already added 1 out of 3 gel well the gel/dye mixture for priming. Do you have to add gel/dye mixture again on that well?

[@mostirreverent]

why is the latter separated into peaks, while the sample is a single peak?

[@raposobernardo]

Hello everyone, how are you? I wondered how much it would be necessary to pay for a small lab capable of doing NGS? Can anyone help me? Thanks in advance! :)

[@sudhagc9796]

cool

[@sajanshrestha1613]

I am a beginner, we are doing sequencing but is not show sequences like atcatcgatgc

[@manuellessi98]

this is not sequencing, it is pre analysis of sample that will be sequenced

[@sajinair870]

Garu of love and Light...🤔🙃

[@anonviewerciv]

3:20 13:20 Software.
5:00 Hardware.

[@romeuribeirodeoliveirafsar7450]

In