Gel Electrophoresis
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- เผยแพร่เมื่อ 7 มิ.ย. 2024
- How exactly do molecular biologists figure out all this stuff we have been learning? How do they do science with huge molecules like DNA and proteins? How can we gather empirical data about such molecules? Let's take a look at one absolutely indispensable technique called gel electrophoresis. This has many applications, but generally we use it to separate mixtures of biomolecules, often DNA, so that we can isolate particular strands for further analysis. How does this work? Let's find out!
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Thank you so much, sir. Your pace of teaching is spot on. At no point does it feel rushed, yet I feel engaged and interested at all times. Keep up with the good work!
I like this professor, he's better than my teachers, and more fun.
Ok
Starting a new job in biochemistry and have only done this once. I appreciate the video.
Man 3 biochemistry an 3 organic medicinal Chem classes later I've never hear what I did so well...thanks Dave you explained its use so mater of fact. I had instructors from France, India, and China and they d just give equipment and show you how to operate it but not how it's operating.
r u taking both biochem and medicinal chem? how r u?
Neat, was always curious about how it worked. 🧬🧫👏🏻
The best teacher ever ❤️
Your videos make everything make sense!!
The intro still kills me
Thank you so much for this clear explanation. ❤
you are just awesome; the lesson was so clear i can't thank you enough and i ask you to keep on doing video in molecular biology thanks a lot
Very good explanation
Ah Professor Dave...
The Jesus of Science,
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Went from Professor Dave to Professor Daddy overnight. Kinda looks like a buffer penguinz0
Good shit, Dr. Dave!
Excellent video...
thank you dave
Very Very helpful. Quick understanding
That's what I do best😉
We did gel electrophoresis in my first freshman biology lab.
A video about 'Gene fingerprinting' or more about Southern Blotting would be much appreciated, thank you.
Beautiful ❤️ lectures I'm going for an exams on Biochemistry on this topic part 〽️ Gel electrophoresis! More lectures on it
i always avoided you due to your channel logo, it always teased me
You are awesome sir... Tku soooo much.
Where were you a few days ago I just had a test question on this 😣
brings back fond memories from biochem lab
Thank you sir
Was studying for my exams and suddenly saw this video, it's great!! Your channel and videos are amazing, thanks for making these contents❤️🔥
Keep striving!
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Great and precise
One of my techniques😉
I remember this one from Bio!
An accurate and interesting explanation, thank you
I did this once in a school excursion. We used our own dna for this.
us too
i love professor dave
Tysm
How to interpret the gel results?
How to know which plasmid has insert or self ligated or why are some results showing RNA?
3:05 & 3:15. The left hand lane contains DNA of fixed sizes to use as a scale. In checking correct insertion you can run a lane with the unaltered plasmid as a comparison or (better) digest some of the new plasmid and check to see if the right size insert appears.
Sir plz can you make a video on drift velocity because it's a quantum phenomenon
Ok I will
New look 😍😍
hi professor dave! i noticed a mistake at ~1:50...the cathode is actually positively charged and the anode is negatively charged! you have this reversed in your explanation!
Common misconception…
Anions are negative, and cations are positive.
However, in gel electrophoresis, the cathode is negatively charged, while the anode is positively charged. (BIOLOGY) You are, I believe, referring to a galvanic cell. In a galvanic cell, anodes are negative and cathodes are positive. (PHYSICS)
Nothing switched, it’s just that there are different perspectives in the two subjects (physics and biology). The SURFACE of an anode is positive and the SURFACE of a cathode is NEGATIVE. However, the CORE of the anode is negative and the CORE of the cathode is positive.
Very cool - thank you :)
Np
last time i was this early, he did not know a lot about the science stuff
The professor is quite smart. 🤯
I am going to have exam in martin physical pharmacy next month especially in biotechnology. Chapter ...... i need help in this exam ... can any one tell me what online lectures will help me ... 🙏 😢😢
Shouldn't the anode be the negatively charged electrode ?
Because there's an electric charge applied, the anode is the positively charged electrode :)
Its called the anode because its where anions move, its unrelated to the charge, it would make sense to be cathode as its cationic but its where anions move so we call it an anode, similarly cations move to the cathode
Wow can you make a flow cytometry or maybe DNA RNA Extraction or PCR
You can extract DNA/RNA from a cut-out section of an agarose gel by dissolving it.
LEGEND
Professor Dave: "this technique can also be used to separate mixtures of proteins according to electrical charge"
SDS-PAGE: "Am I a joke to you?..."
That’s very true, any objections to that?
Non-denaturing PAGE.
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Isn't anode is negatively charged?
Nope, the positive anode attracts anions toward it, therefore it is a positively charged electrode :)
the location of plasmid DNA in agarose gel can be visualized by ethidium bromide or bromophenol blue??!
What’s the difference between them?
EtBr will (with UV) but bromophenol blue is just a marker dye that tells you the gel is running OK.
Wish my teachers were as good as u 😭
haha
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But bigger DNA also has a bigger negative charge, and should have a bigger force acted on it. Why doesn't it make it move farther?
Even though it has bigger negative charge it will have more molecular weight.
Because the negative charges are evenly spaced along the DNA backbone, and the number of charges are proportional to the length, the DNA receives a very even force per unit length. As a result, the migration is affected mainly by the difficulty in sieving through the gel pores.
Where the charge of the molecule has a big effect is in Native-PAGE protein electrophoresis, since a heavily charged protein will be pulled through faster. In SDS-PAGE (the first picture Dave showed with the bright blue sample wells was SDS-PAGE) the protein is solvated by negatively charged detergent, so it acts mainly on size.
i am the first viewer! yay!
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Sir I'm not getting video s in gmail
The videos are on TH-cam, bud. If you subscribe and click the bell for notifications, it will tell you when I upload.
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How do we know if the food product is GMOs or not by using gel electrophoresis?
Who sings the intro every time
I know some of the words.
Thomas and his friend inspired intro......
Ltc
Isn’t anode (-) and cathode (+)?
I think you confused anion with anode.
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second viewer yay
I hated high school biotechnology thanks to the psychotic teacher I had who sprayed us with di water :(
1.25 speed
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First!
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