how Crispr Cas9 works? Crispr A Gene Editing Technology | Video 2
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- เผยแพร่เมื่อ 29 ส.ค. 2024
- The essence of CRISPR is simple:
it’s a way of finding a specific bit of DNA inside a cell. After that, the next step in CRISPR gene editing is usually to alter that piece of DNA.
However, CRISPR has also been adapted to do other things too, such as turning genes on or off without altering their sequence.
The key to CRISPR is the many flavours of “Cas” proteins found in bacteria, where they help defend against viruses. The Cas9 protein is the most widely used by scientists. This protein can easily be programmed to find and bind to almost any desired target sequence, simply by giving it a piece of RNA to guide it in its search.
When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a 20-DNA-letter long sequence that matches part of the guide RNA sequence.
That’s impressive, given that the DNA packed into each of our cells has six billion letters and is two metres long. What happens next can vary. The standard Cas9 protein cuts the DNA at the target. When the cut is repaired, mutations are introduced that usually disable a gene. This is by far the most common use of CRISPR. It’s called genome editing - or gene editing - but usually the results are not as precise as that term implies.
CRISPR can also be used to make precise changes such as replacing faulty genes - true genome editing - but this is far more difficult.
#crispr #crisprcas9 #crisprcas9explained
Apki vedios dakh Ka ya andaza huta ha ka study itni interesting bhi hu Sakti ha love to watch your vedios❤❤❤
Sir u are fav sir every time I watch ur video it help me a lot sir...ur teaching level is outstanding. ...u explain so clearly 👍
Wow sir
Itna deeply samjhaya wo bhi easy language me...😊
Thank u so much sir 😍❤
Excellent, Dr. Abdul Rahman. If these videos could be Arabized, it would benefit many students. That is, converting the video into Arabic while preserving the scientific terminology.🙏🙏
Thank you peyary bhai❤
Sir bhout acha concept clear Kiya ap nay .ALLAH pak give you more success. Stay blessed and please keep it up . Send more lecture related to BIOTECHNOLOGY.
Great lecture ♥️
Thankyou so much,Your video helped me a lot to understand this topic
Thankyou so much sir so good lecture
Brilliant brilliant brilliant brilliant 👍👍👍👍👍👍👍👍👍
Love from Bihar India
amazing
Great lecture
Amazing sir 😍 well explained Thank you ✨
alhumdulillah well understood ,,,Allah bless you
Thank you sir it is very informative 🙂
Tum bohot achaa Kam karte mia
Perfect explanation 😇
The spacer region that is complementary to viral DNA my question is that every virus have a region that is complementary to that spacer region? Second question spacer region sequence is same in every bacteria?
Thanku much sir
Bro I need title for masters research on CRISPER CAS9
Antibiotic resistant gene aur pencilin aur explain kare
sir can u tell that how can we knockout a gene in a crop using this technique.
Kaya kisi bhi type ke bacteria se hum genome editing kar sakte hai
Minjesuf apke samaj ata ha, I am ph D student
Sir can u provide me the notes please.....
❤
sir you are a good teacher and belong to a great profession like teacher but this kind of shirt doesn't suit with you and your personality , if i am wrong correct me
Right
i think its very too weather and using a fan will degrade the audio quality- so i think maybe thats why he wore this shirt
Please made a video on 5 types of kingdom classification and types of bacteria
I am first
virus ke bhai dna nahi hota
Sir ji ap body dikha rhe hain 🙈