Membrane Filtration Technique for Water Analysis (E. coli, Salmonella, Pseudomonas, Coliform etc.)
ฝัง
- เผยแพร่เมื่อ 22 พ.ค. 2024
- The Membrane Filtration Technique was introduced in the late 1950s as an alternative to the Most Probable Number (MPN) procedure for microbiological analysis of water samples. It involves the use of membrane filters, which are thin porous sheet structures composed of cellulose esters or similar polymeric materials. This techniques or method is applicable when the number of bacteria in a water sample is very low.
This video represents a detailed procedure for the enumeration of E. coli, coliform and other microorganisms using the membrane filtration technique.
__________________________________________________________________________________________
Follow us on Social Networks to Stay Connected:
TH-cam: / @microchemsexperiments
Facebook Page: pg/MicroChem...
Be with us, be a Scientist. ______________________________________________________________________________________________
Music Credit:
Audio Library: • Video
TH-cam Audio Library: • Roadtrip - Scandinavia... __________________________________________________________________________________________
Related Tag:
#membranfintrationtechnique
#membranfintration
#microbiologicaltestofwater
#watertest
Thank for this video. So informative and another technique is added to my list
Can not get over how amaizing ur explaination is...all r grateful for that massive work 🌹🌹🌹🌹
Thanks a bunch
This is beautiful and so easy to comprehend.
Thanks a lot
You are welcome. Stay with us
The explanation was clear, concise and informative, I learnt all I needed to for a project Im working on, thank you!
Thank you so much
Send email for get help/suggestions about your practical knowledge (mail.mic.chem@gmail.com)
This my job at Nestle :) Living the dream!
Great !!!
Are you from India?
Can u tell me if there is any vacancy available in Nestle?
So you only perform water testing?
what's the package bro...
Great! Thank you...But I would like to know if you worked with hydrophilic or hydrophobic membranes
You are talented and delivers the lecture very smooth Thank you so much
Thank you so much. Stay with us
th-cam.com/video/uwgT65EiY1Q/w-d-xo.html
watch this video of membrane for water purification
Very nice video
Brilliant..Continue the journey of knowledge.
Thank you, I will
Thank you dear.Can you please mention the specification of filtration unit?
Nice.i. appreciating & thanks all of you for giving me scientific knowledge regarding to microbiology & Micology in general.
Our pleasure!
Is it normal to observe something at the extremities of the filter ? Are they growing colonies ? Or do some metabolites reacts with the medium ? Or is it something else ?
Thanks for explaining
You're welcome
Thank you, I understand it clearly.
Thanks for staying with us
th-cam.com/video/uwgT65EiY1Q/w-d-xo.html
watch this video of membrane for water purification
Thank you great bexplaination
Glad you liked it
Thanks
Very informative Video ....
Glad you liked it
Please make a video on MPN in a similar manner
Amazing experiment 🙌
Thank you. Keep supporting us.
best wishes from my water chemistry lab
GOOD EXPLANATION:)
Thank you.
Nicely explained...
Thanks. Stay with us
Good introduction. Can we put the filter paper into the liquid media in the flask after filtration instead of the Petri plates for growing the bacteria?
Yes. You can.
Hi, I have a question about colonies on CCA chromogenic coliforms. Can we extend the incubation?
Sometimes yellow colonies appear after 24 hours, which become pink with longer incubation.
In this case, are there also coliform bacteria?
Which Agar you would suggest for counting colonies at 22 and 36 degrees?
This video is amazing mam, but I have a question, please reply. Actually, you told that E coli will appear blue in color on this media, but it is a gram negative bacteria. Then it should appear pink in color. Please tell me why this happens.
cant you use M -Fc agar LES for E.coli and M Endo Agar LES for total coliform organisms???by seeing this i can feel that results would be very hard to take
Thanks for video
You are welcome
Very nice, I really like it. just one rectification. It says incubate the plate @35 degree but in the video it showed putting the plate in the hot air oven.
We are using the Hot Air Oven as incubator.
Please upload the video of legionella detection
Nice video ! Please can this technique be used for testing of Ecoli,Coliform,salmonella,pseudomonas and staphylococcus organisms present in sanitary pad. And if yes will it involve just diluting the sample in water ?
Yes. This method can be applied after diluting your sample in sterile distilled water if the expected microbial count is low.
But if the expected microbial count in your sample is high, then you have to follow different method as I have already uploaded for enumeration of E.coli, coliform & salmonella from different sample. Please find those tests from my channel.
Electrospun PVDF-carbon membranes preparation for water filtration. Pls watch our video
th-cam.com/video/VoBOMrVbDyU/w-d-xo.html
Thanks for the video.. can u explain and do a video on water sampling , normally checking for water quality in Industries
We will make video later. Thank you
What is the name of this tool? Where we can buy it?
Amazing.
Thank you
So plz how do you calculate for CFU. If the colonies are 180
Thanks for the video. Can u show me details preparation for the negative and positive control plz?
Ok. We will make video later
thank you
You are welcome
Can you pls share with us the method of the most probable number technique (MPN) also?
Ok, we will make video on it soon. Stay with us.
Hello. Thanks for the video. It was nicely explained. I would like to ask that how will we determine the water quality using this filter membrane method and what are some disadvantages of using this method.
If pathogenic bacteria is found, means that the water is bad in quality. Only disadvantage is that it is applicable for water sample with low bacterial count
@@MicroChemsExperiments Thank you so much. Really appreciated.❤
Please suggested another techniques for purified water testing
Electrospun PVDF-carbon membranes preparation for water filtration. Pls watch our video
th-cam.com/video/VoBOMrVbDyU/w-d-xo.html
th-cam.com/video/uwgT65EiY1Q/w-d-xo.html
watch this video of membrane for water purification
Nice video....plz make a video on multiple fermantation tube techniques for fecal coliform .
yess agree👆
Noted the parameter. We will work on it soon. Stay with us.
Nice video
Thank you
Thank you so much mam
Most welcome 😊
Is it good topic for msc project?
The filtration unit set consist of bottom, middle and top parts. The bottom part is collection flask and the top parts is funnel. What is the middle part of unit filtration unit called? Thank you
Middle part is called basement
Well explained do you have any video colony counter working
We dont make video on instrument. In future, we will make video on different instruments. Stay with us.
We used colony counter in coliform count. Take a look
Thanks for the video
Can you please do for testing for presence of yeast and mold in sample using this membrane filtration technique
The same procedure will be followed except DRBC agar plate should be used replacing the Chromocult Coliform Agar and incubate at 25 degree Celsius for 3-5 days.
Total fungal colonies on membrane filter is the total yeast & mold.
@@MicroChemsExperiments and preparing the yeast and mold using distilled water ! Does it involve using peptone water ?
@@theophilust9736 Using normal physiological saline (0.85% NaCl)
You guys are awesome ! Love that you are willing to help
I am trying to set up a lab for sanitary pad and I need agar to buy
I will be testing for the presence of ecoil&coliform, yeast&mold, salmonella,staphylococcus aureus, pseudomonas aeroginosa, total viable count. Obviously I now know using membrane filtration is easier but knowing the appropriate selective agar for each is where I need help. I know for ecoli/coliform from this video and for the fungi through your response to my question. Please list the agar for salmonella, pseudomonas euroginosa,staphylococcus aureus and total viable count . I appreciate you
@@theophilust9736 Buy XLD agar for Salmonella, Nalidixic Cetrimide Agar for pseudomonas aeruginosa, Plate Count Agar for Total viable count and Mannitol Salt Agar for Staphylococcus aureus.
Thanks, one question: if the filter paper is not inverted while placing on agar, how the filtered microbes present at the top side of filter come in contact with the media?
Microorganisms absorb nutrients from the media through the semi permeable filter paper
@@MicroChemsExperiments Thanks!
Good.
Thank you
excellent information. can you help me with CFU count? im doing testings on non sterile cotton
Surely I can help you. Please write in details.
What agar plate that you used to measure the e coli
Chromogenic Coliform Agar
Hey, could you tell me why you "don't invert plates during incubation". If you have some references to this method I would be appreciate if you could share. Thank you.
Hi, I feel if we incubate it in an inverted position, the filter paper may fall off from the media side of the plate.
You shouldn't invert the plate because filter paper is on the surface of the media. Reference: Iso 9308-1:2017
@@MicroChemsExperiments thanks for the useful info, but as in ISO 9308-1/2014: after filteration, place the membrane filter on the agar. ensuring that no air is trapped underneath, invert petridish and incubate at 36 *c for 24h!
hi, i have a question. does the filtration appratus need to be autoclave for each sample? Thank you
Yes
Can we do different filter membrane filteration for the same solution??
You can
Hiw do we incorperate enrichment of the sample into this process?
Enrichment is not needed and not possible
Do you know what is the composition of chromogenic coliform agar media? Could you give the tutorial how to make it?
We know the composition but making in local lab is difficult
Hi, i have a question? How do i sterilise the agar medium since it can't be autoclaved?
No need to sterilize it. Just dissolve the media as per the instruction mentioned in the label or catalog of the media
What is the active ingredient that is responsible for giving color to colonies?
Different media have different ingredients and give different colored colonies. Please study about media composition in Google
In this viedo you have told that incubate the petri plate without inverting it ,but in ISO 9308-1 it there to invert the petri plate.
Which is correct please make it sure.
Filter paper is on the the culture media of the petri plate. So the plate should not invert
If we cannot count the number of "blue colonies", & "blue & purple colonies" we have to do dilution before filtering. So in that case, how to do dilution ? And how to do calculations ?
Plzz explain.
Thanks.
For dilution: mix 1 sample with 99ml sterile distilled water.
Calculation: Number of colony × 100
Plz explain 🙏
Is there any method to sterilize the membrane filters if they are not sealed?
If the filter autoclavable, then autoclave it to make it sterile
Membrane filtration technique for water samples using chromocult agar I observed some transparent colonies apart from pink and blue colonies. So, I want to ask what organism is that transparent colonies. Can u explain.
You can use differential media and then conduct biochemical or serological experiments to confirm those colonies.
Can the liquid still go thru the filter without using a vacuum pump?
No. Water can't pass without using vaccume pump
you say, for the preparation of the chromocult coliform agar, we need to dissolve 5.5 g of dehydrated power of agar, but you had written 2.55 g of to dissolve in 100 ml distilled water. Can you please clear me my confusion?
Ops sorry, we did mistake while saying. It should be 2.55g for 100ml.
Thanks for the explanation. But can this method be used for contaminated food?
No. This method is not for the food sample
@@MicroChemsExperiments i see. Can you suggest what method can be used for food sample?
I have question. For membrane filtration technique, it is advisable to filter 3 different volume. Do i need to sterilised the filtration unit in between filtering 3 volume or i can filter 3 different volume using the same filtration unit?
Sterilisation for 3 times is best but you can sterilise only once for 3 volumes of sample as you are taking same sample.
@@MicroChemsExperiments thank you so much for your answer
@@redvelvet191 you are most welcome
can we use VRBA/EMB for Colifrm/E. Coli & PDA for Yeast/Mold?
Yes you can
Can this method be used for food samples such as sugar and others?
No. This method is applicable only for Water sample
Nice❤❤
Thanks 🤗
@@MicroChemsExperiments
Electrospun PVDF-carbon membranes preparation for water filtration. Pls watch our video
th-cam.com/video/VoBOMrVbDyU/w-d-xo.html
How could you use filter paper without pre wet
Try as shown in this video. It will work
So how are the Membrane Filters Sterilised? They don't seem to be Sterile.
It is sterilized by Autoclaving
@@MicroChemsExperiments do you put them in aluminium foil for autoclaving?
Can I use nutrient agar for this analysis?
Yes, only if you want total plate count from your sample
Can I repeat the exact same process for Salmonella but replacing chromcult Agar with XLD and keeping all the steps same?
Also how to calculate the number of colonies for it? the same way?
Yes you can, and the calculation will be the same.
@@MicroChemsExperiments Thank you so much!
i could not find XLD, so using Salmonella Shigella Agar now, hope it works in the same way.
what is the Water testing guideline
Many guidelines can be found for water testing. Like American Water Association
Can i ask you?, Which regulation that you follow?? APHA or As??
ISO 9308
Voice very sweet
Thanks a bunch
Is the measuring cylinder sterile ?
Yes.
as per which standard u have performed these tests..
ISO
how much do the total test parts cost?
Varies with the type and brand of the used media and equipment
At how many count of coliform indicate water potablity?????
Zero coliform per 100ml drinking water
sulphate reduction anaerobic bacteria test (water) vedio upload mam plz
Noted
Mam we can use alternative M-indo broath.
No.
If Colonies are growing outside the membrane that is below the membrane ,then what is the problem Mam?
It indicates cross contamination. Don't count them during result interpretation. only count colonies on the filter paper
@@MicroChemsExperiments thank you
Mention the oil content test procedure
Which sample?
@@MicroChemsExperiments bleaching earth oil content. Vegetable oil
We already uploaded.
Video link: th-cam.com/video/24qWVLm7hhw/w-d-xo.html
Is it .22 or .45 micron filtr??
0.45
For heterotrophic count which culture media is used?Nutrient Agar is enough?
Yes. Nutrient Agar.
@@MicroChemsExperiments Thank you.
One more thing,For Fecal streptococcus and sulphite reducing bacteria which culture media is used?
@@sandhyaranjith427 Bile Esculin Azide Agar is used for Fecal streptococcus amd Tryptose-sulfite-cycloserine (TSC) agar is used for Sulphite Reducing Bacteria
@@MicroChemsExperiments Thank you so much.
@@sandhyaranjith427 You are welcome.
No need to autoclave the medium?
No. No need to autoclave
Is this filter paper sterile?
Yes
spr mam
Thanks
👍
❤️❤️
hello can you help me ?😊
How can I help you?
Thought I was lucky to have found a technical video for exactly what I need.
>Indian voice
>sigh
3:18
Which problem with it?
Please wear proper gown
You are correct. We should wear full sleeves gown in laboratory
Thanks
Very nice video
Thank you