TLC (thin layer chromatography) of pigments from spinach

[@Explore_with_ZY]

perfect one. Great job, you just showed the whole procedure with such a simplicity.

[@konstantindimov3153]

Hello, are you using silica gel or paper as a stationary phase?

[@lilibeldelapuente2163]

Did you centrifuge the bottom layer?

[@saptarshisen007]

What is 2nd solvent mixture added? Time 1:06 sec???

[@Zoe-sh3hl]

Hexane I think

[@sourabhbhartia5241]

What is the chemical used other than acetone while sample preparing

[@allispracticalscience503]

it is hexane

[@evamoses9720]

@@allispracticalscience503 why do you mix hexane and acetone when grinding the leaves? what is the purpose?

[@allispracticalscience503]

@@evamoses9720 Leaves contain many different pigments for photosynthesis. Some of these are more soluble in hexane and others are more soluble in acetone. By using a mix of both you are more likely to extract all the different pigments

[@evamoses9720]

@@allispracticalscience503 thank you!! ☺️☺️

[@Idk-ij1yy]

thanks so much!

[@andrewlight1492]

thank you so much 😊😊

[@tergelmaasukhbaatar8331]

when i did it in school used the grass with sand i dont know what will the sand do tho

[@allispracticalscience503]

The sand will help to grind down the leaf structure and break open the cells so it is easier to extract the photosynthetic pigments

[@sid_rawat944]

Tqsm ❤️

[@uzmaawan4867]

Kindly share notes

[@Idk-ij1yy]

Hey is there any way to improve this method