Thin Layer Chromatography (TLC)

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  • เผยแพร่เมื่อ 31 ธ.ค. 2024

ความคิดเห็น • 146

  • @skxjxhxh
    @skxjxhxh 5 ปีที่แล้ว +368

    You just summarized our 3-hour lesson into 9.5 minutes

  • @masskiller9206
    @masskiller9206 4 ปีที่แล้ว +70

    7:52 "It’s usually difficult to predict what will work best, so just try something and see what happens. " professor dave motivates

  • @ramil8029
    @ramil8029 5 ปีที่แล้ว +101

    Getting ready for the MCAT exam. Your videos are THE BEST on youtube for all of ochem and gen chem material! Thank you so much!

  • @drewkrahn
    @drewkrahn 3 ปีที่แล้ว +68

    So much easier to understand than our lab manuals. Thank you!

  • @nobody.of.importance
    @nobody.of.importance 3 หลายเดือนก่อน +5

    Was watching a video on NileRed about extracting urea from his piss, got curious how recrystallization works, looks it up, finds other separation techniques, doesn't understand what it means by "phase", and now I'm here. Chemistry's nuts. I'm not a student, at least not of any classes, I just really like learning about how things work, and your videos are super helpful. Thanks Dave

  • @iuliiamairansaeva9169
    @iuliiamairansaeva9169 3 ปีที่แล้ว +4

    I'm happy you've decided to do youtube... Some of us watching you right now will become great chemists in the future, and you'll be one of the main reasons we'll manage

  • @lalaland774
    @lalaland774 4 ปีที่แล้ว +31

    Since we’re on quarantine my Organic Chem lab professor is using your videos and basing the work he gives around your videos

    • @danielmeaders4005
      @danielmeaders4005 4 ปีที่แล้ว +9

      This honestly seems like an issue of legality and compensation. The situation is the same at my university (however not for this video), I don't think your school is writing checks to professor Dave... but they are certainly getting their monies.

    • @XTheSpartanX7
      @XTheSpartanX7 4 ปีที่แล้ว +1

      i wish mine did that

    • @jameshall1300
      @jameshall1300 ปีที่แล้ว

      @@danielmeaders4005 it's probably kind of new legal ground to be honest. Before quarantine happened and e-learning became almost the de facto standard in most places, using internet resources so heavily in class was probably very limited. I'm not sure ad revenue really would be fair compensation for that type of usage.

  • @meganevans8006
    @meganevans8006 3 ปีที่แล้ว +86

    I don't know why o-chem lab manuals have to be mystery novels. Thank you for your videos!

    • @Andy_M.S.c
      @Andy_M.S.c 3 ปีที่แล้ว +2

      Mystery novels 😭😂😭

    • @patiencechepkorir5596
      @patiencechepkorir5596 2 ปีที่แล้ว +2

      I Know right 😂😂

    • @squilliamfancyson5313
      @squilliamfancyson5313 2 หลายเดือนก่อน

      I hate the one that's a smart ass about everything. It's called like the organic chemistry lab survival guide. I absolutely despise that stupid manual because it's not funny or clever at all. It's annoying and talks down to you. It would be one thing if you already had prior experience in a lab, and it's poking fun if you mess up, but almost everyone hasn't even been in a lab before. And if they have, most of the stuff was already set up for them.

  • @Alex-gb4vn
    @Alex-gb4vn 4 ปีที่แล้ว +16

    DAVE. Keep up the good work. Thank you so much for your contributions. You really explain things in a manner that is so straightforward and easy to follow. I appreciate you so much.

  • @nikkaagbayani8905
    @nikkaagbayani8905 4 ปีที่แล้ว

    Mahal na po kita Professor Dave. Ikaw po ang pinakadakilang guro na nakakapagpaintindi saakin ng mga bagay na mahirap intindihin.

  • @Senkuyar
    @Senkuyar 11 หลายเดือนก่อน

    I'm an msc student and you helped me pass my enterance exam and now helping with my final exams in grad school, you're a life saver! :')

  • @veronicaaguiniga8496
    @veronicaaguiniga8496 8 หลายเดือนก่อน

    Being a visual learner instead of trying to understand material that needs to be read, I feel that this video on TLC should be shared with students that would like a better insight/understanding on this topic. From watching this video I actually learned that the TLC plate itself has hydroxyl groups that is dipole-dipole, allowing it to interact with other polar substances.

  • @mr_mars_vr
    @mr_mars_vr 2 ปีที่แล้ว +2

    Thank you for this.

  • @sarahholland5980
    @sarahholland5980 4 ปีที่แล้ว +4

    thank you! prepping for lab quizzes using your videos bc they are so much easier to understand than our manuals

  • @PG-qn8od
    @PG-qn8od 5 ปีที่แล้ว +3

    Jesus, man, your oc videos are helping me sooo much right now, thank you a lot! ❤️

  • @R_samurai779
    @R_samurai779 4 ปีที่แล้ว +1

    Thanks a lot man! Our schools told nothing about how this is typically used as an analytic technique

  • @sarahmitchell8344
    @sarahmitchell8344 4 ปีที่แล้ว +1

    This has changed my life

  • @mahmoudothman7910
    @mahmoudothman7910 5 ปีที่แล้ว +43

    Nice new hair-cut Dave 😊

    • @siddheshdeshpande3034
      @siddheshdeshpande3034 3 ปีที่แล้ว

      Avadli ka tula

    • @Jdoug305
      @Jdoug305 3 ปีที่แล้ว

      @@siddheshdeshpande3034 yes I like

    • @siddheshdeshpande3034
      @siddheshdeshpande3034 3 ปีที่แล้ว +1

      @@Jdoug305 mr cheeks from Maharashtra

    • @Jdoug305
      @Jdoug305 3 ปีที่แล้ว

      @@siddheshdeshpande3034 aklakam brother 😁

  • @pallobbarai4152
    @pallobbarai4152 10 หลายเดือนก่อน

    Thank you, your lecture is amazing, easy to understand, animation is excellent.

  • @BIAKANOORWorld
    @BIAKANOORWorld 5 ปีที่แล้ว +3

    always satisfied with your explanation prof., a huge fan of you from India

  • @ginamarie7603
    @ginamarie7603 4 ปีที่แล้ว +1

    great for visual learners, finally a video that makes it easy to understand. thanks.

  • @sohampowdwal8180
    @sohampowdwal8180 2 ปีที่แล้ว +2

    in professor dave we trust

  • @FullNanochem
    @FullNanochem 2 ปีที่แล้ว

    Спасибо!

  • @bhawanagahlot9540
    @bhawanagahlot9540 4 ปีที่แล้ว

    Explaination which u gave is amazing.. u have professional teaching skill...

  • @user-kr4ns9vk7j
    @user-kr4ns9vk7j 3 ปีที่แล้ว

    Hello Doctor
    I am now working on an experiment, which is to prepare Schiff bases and run by escalation with the condenser for no less than 6 hours, and I want to follow the end of the interaction with the technique TLC (The solvent used for the reaction is ethanol
    The question is what polar and nonpolar solvents can be used in chromatography and what ratio shows the endpoint of the reaction )

  • @ayasamer7731
    @ayasamer7731 4 ปีที่แล้ว

    At the morning of my exam .. soo helpful a huge thanks to u ❤

  • @ViperChief
    @ViperChief หลายเดือนก่อน +1

    Bloody life-saver

  • @mawadaabdalaal9916
    @mawadaabdalaal9916 2 ปีที่แล้ว

    شكراً جداً بسيط و مختصر ❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️

  • @emteemte3985
    @emteemte3985 2 ปีที่แล้ว

    U r unique .i take lab abt tlc anc CC and you make it easy and clear thank you. I wish you good health and long life

  • @gabyyoga8385
    @gabyyoga8385 4 ปีที่แล้ว +1

    Incredibly easy to understand thank you so much!

  • @mmhh3553
    @mmhh3553 7 หลายเดือนก่อน

    May Allah bless you and guid you to the all right thing here and and hereafter,,, all the best

  • @BossRotah
    @BossRotah 5 ปีที่แล้ว +5

    Hello Dave, please talk about Keto Enol Tautomerism

  • @chemistry7554
    @chemistry7554 5 ปีที่แล้ว +2

    Good personality with hair cut,by the way vedio is help full............

  • @vvideo7162
    @vvideo7162 2 ปีที่แล้ว

    Wow sir it's an amazing lecture I have ever seen thanku so much sir ,for clear my doubts 🥰🥰🥰🥰🥰

  • @karenopt
    @karenopt 4 ปีที่แล้ว +4

    If the solvent is polar, then the polar molecules will have a smaller Rf and non polar molecules would have a larger Rf? Or is it the other way around?

  • @vintilovwhitefox5365
    @vintilovwhitefox5365 ปีที่แล้ว

    Thank you dude, it helped me a lot

  • @sweetoldsoul4454
    @sweetoldsoul4454 3 ปีที่แล้ว +1

    Thank you 🙏

  • @elisophiaaa
    @elisophiaaa 4 ปีที่แล้ว

    well and a simple-explained tutorial. tnx, prof.!

  • @ValentinaLopez-bv6ro
    @ValentinaLopez-bv6ro 4 ปีที่แล้ว

    Perfect explanation. Thank you

  • @ARBB1
    @ARBB1 5 ปีที่แล้ว +240

    *Who are you, and what did you do with Jesus?*

    • @-Noushin
      @-Noushin 4 ปีที่แล้ว +1

      🙄👉😐👉😑👉😀👉😁👉😂👉🤣

    • @alphagonist4748
      @alphagonist4748 4 ปีที่แล้ว +4

      👍🤣😂🤣👍🤣👍🤣💪👎🍌

    • @muhammadumarsarfraz4705
      @muhammadumarsarfraz4705 ปีที่แล้ว

      What???

    • @c.u.v2190
      @c.u.v2190 11 หลายเดือนก่อน +1

      Pinned him

    • @jjmeducationcorner1358
      @jjmeducationcorner1358 9 หลายเดือนก่อน +5

      Jesus is a real savior of the world

  • @mohinikulkarni4129
    @mohinikulkarni4129 2 ปีที่แล้ว

    I loved watching this TLC 👍

  • @natehcsingh8634
    @natehcsingh8634 3 ปีที่แล้ว

    Came in jee mains 2021 february attempt . Thanks Jesus↗↗

  • @anounymous7988
    @anounymous7988 2 ปีที่แล้ว

    You're amazing bro god bless you

  • @aang7505
    @aang7505 4 ปีที่แล้ว +2

    thank you so much! I just wanted to ask, do you benefit more from patreon or from TH-cam channel memberships? I really appreciate your help, this explanation was so clear

    • @ProfessorDaveExplains
      @ProfessorDaveExplains  4 ปีที่แล้ว +4

      Patreon takes a smaller cut so that is much better, thanks so much for your support!

  • @ParedonesAmarillosNO
    @ParedonesAmarillosNO 5 ปีที่แล้ว +2

    What solvent would you use to prepare the samples for cannabidiols. Thank you for your class! Love learning.

  • @moonlillac
    @moonlillac 2 ปีที่แล้ว

    thankyou, it help me so much.

  • @superhessu3
    @superhessu3 2 ปีที่แล้ว

    4:56 just marking for my upcoming university course

  • @huntersidell
    @huntersidell 2 ปีที่แล้ว

    Amazing video, thank you so much!

  • @mariamgad284
    @mariamgad284 5 ปีที่แล้ว +1

    very excellent 💙💙

  • @chorgulio
    @chorgulio 5 ปีที่แล้ว +2

    So what is the typical solvent used for TLC? would that depend of the sample/compound to be analyzed?

    • @ProfessorDaveExplains
      @ProfessorDaveExplains  5 ปีที่แล้ว +1

      in the lab i would typically use a mixture of hexanes and ethyl acetate. it's kind of personal preference to an extent.

  • @Ambient_Scenes
    @Ambient_Scenes 4 ปีที่แล้ว +1

    Thank you for the video! One question: If you have no clue what your sample is made of, how do you know whether to use UV light, or a stain to visualize the dots? Also how do you choose the right dye? Also, what do you do if some components of the mixture only get stained by on stain, while other components only get stained by another stain?
    Is there a huuuuge amount of different stains the organic chemist has to experiment with or are there just a few that will cover pretty much most chemicals?

  • @Leen-ih8ql
    @Leen-ih8ql 3 ปีที่แล้ว +1

    what are the methods or ways of separating plant pigments?? only TLC and paper chromatography ?? please I need help

  • @ihechisomakagha2568
    @ihechisomakagha2568 ปีที่แล้ว

    Just Thank you ❤

  • @asmaaaboalmagd792
    @asmaaaboalmagd792 8 หลายเดือนก่อน

    Thanks professor

  • @roseycheekies51
    @roseycheekies51 3 ปีที่แล้ว +1

    i love you dave

  • @HeyImLucious
    @HeyImLucious 5 ปีที่แล้ว +2

    If only this video came out a few years ago... I wouldn't have looked like such a fool in my research lab haha

  • @chemistryforsociety3448
    @chemistryforsociety3448 9 หลายเดือนก่อน

    Excellent...... thanks a lot

  • @prateekkulkarni2639
    @prateekkulkarni2639 8 หลายเดือนก่อน +7

    A day before jee mains 2024

    • @marniejones331
      @marniejones331 7 หลายเดือนก่อน +1

      Kya mtlb Indian log smart rahte hai

  • @kikimiki177
    @kikimiki177 2 ปีที่แล้ว

    is the polarity of the samples the only thing that impacts how far each of them will travel, or does their relative mass also have an impact?

  • @lyricmelody4650
    @lyricmelody4650 4 ปีที่แล้ว

    This is so helpful! Thank you :)

  • @137bob3d
    @137bob3d 5 ปีที่แล้ว +1

    superb presentation. and good of you to cite how TLC is a stepping stone to column chroma'
    ... and to work out the solvent(s) x:y ratios w/ TLC for upper half separation.
    eager to see the col. chr. vid's on any time-correlation in top to bottom flows vs TLC.
    and how you go about collecting the eluent with the separated product and nothing else.
    also will be listening for info on the details of how one chooses a silica mesh size. and possible re-usability of silica for those of us who know how costly that stuff is.
    and lastly how to choose column size vs number of grams of material to be separated.

  • @Iiiiionnersutylllk
    @Iiiiionnersutylllk 9 หลายเดือนก่อน

    Thank u❤❤❤

  • @pintukumargupta8415
    @pintukumargupta8415 ปีที่แล้ว

    Nice easy to understand thaks

  • @tarahclue6737
    @tarahclue6737 3 ปีที่แล้ว

    Thank you

  • @luftatmer
    @luftatmer 4 ปีที่แล้ว

    man that was a great video!

  • @mustakim2144
    @mustakim2144 3 ปีที่แล้ว

    Thank you!

  • @rassimsimou1594
    @rassimsimou1594 2 ปีที่แล้ว +1

    Good

  • @Thaumius
    @Thaumius 5 ปีที่แล้ว +3

    Will you be doing HPLC/GC tutorials?

    • @ProfessorDaveExplains
      @ProfessorDaveExplains  5 ปีที่แล้ว +1

      i hadn't planned on it, but i'll put it on the list! it is a big one.

  • @carolynenglish3068
    @carolynenglish3068 3 ปีที่แล้ว

    Brilliant!

  • @cesca3994
    @cesca3994 5 ปีที่แล้ว

    well explained! Thank uuuuuuu.

  • @yasigerman731
    @yasigerman731 ปีที่แล้ว

    thank you thank you thank you

  • @DapaChrons
    @DapaChrons 5 ปีที่แล้ว

    New haircut! looking good either way

  • @sagagayathri9570
    @sagagayathri9570 5 ปีที่แล้ว

    I had a doubt.... how to know what component has been seperated...and also what is the use of rf value

  • @elizaahmed2066
    @elizaahmed2066 10 หลายเดือนก่อน

    Sir my compounds are non polar compounds. so which solvent do I use for dissove the compound and what solvents do I use in the mobile phase? sir can you tell me a little bit? I am facing a lot of problems in tlc checking

  • @saiteja6742
    @saiteja6742 5 ปีที่แล้ว +1

    Sir why do we use organic solvent(toulene) as mobile phase in thin layer chromatography ?? Pls reply for this sir

  • @YouMockMe
    @YouMockMe 4 ปีที่แล้ว

    I came back to this a few times haha... great vid

  • @nirmanivimarshana9723
    @nirmanivimarshana9723 5 ปีที่แล้ว +2

    nice

  • @Ambient_Scenes
    @Ambient_Scenes 4 ปีที่แล้ว

    Why do you need a capillary tube to place the solution mixture on the plate? Can't you just use a pipette or dropper or something?

    • @ProfessorDaveExplains
      @ProfessorDaveExplains  4 ปีที่แล้ว

      the capillary tube is much better for applying the solution because you press it against the plate and it is adsorbed, a dropper would be terribly imprecise

    • @Ambient_Scenes
      @Ambient_Scenes 4 ปีที่แล้ว

      @@ProfessorDaveExplains Thanks for clarifying.

  • @vibscreationvideos4884
    @vibscreationvideos4884 4 ปีที่แล้ว

    1 ch number bhava ty very much😇🤗

  • @specialparadise
    @specialparadise 5 ปีที่แล้ว +2

    Wow what a haircut Professor! You look good either way lucky you.

  • @esmeralda804
    @esmeralda804 ปีที่แล้ว

    ME ENCANTAAAAA❣

  • @benzjoshwade3462
    @benzjoshwade3462 3 ปีที่แล้ว

    Hello. This might be a dumb question but why does Silica (SiO2) have hydroxy groups?

  • @tanzimnishad3892
    @tanzimnishad3892 2 ปีที่แล้ว

    Sir How can I separate a mixture with 37 compounds in it using column chromatography

  • @fatimasaid648
    @fatimasaid648 4 ปีที่แล้ว

    I’m confused to why the dots are not being places with marker but are placed with a capillary tube ??

  • @ayaalhashimi3100
    @ayaalhashimi3100 2 ปีที่แล้ว

    Please what mean tlc its same column ?

  • @TorsaPal
    @TorsaPal หลายเดือนก่อน

    Dave amake ektu daona oi paper chromatography r video tah...tumi khub bhalo porao

  • @kennyyong7694
    @kennyyong7694 4 ปีที่แล้ว

    Excuse me, do you know how to prepare silica gel to absorb moisture or silica gel to act as the stationary phase in thin layer chromatography. I need to make my own TLC plate

    • @ProfessorDaveExplains
      @ProfessorDaveExplains  4 ปีที่แล้ว +1

      Hmm no I've never made plates before. But check the tutorial that follows this one on column chromatography, it may help a little.

  • @kamiVeTODO
    @kamiVeTODO หลายเดือนก่อน

    Yay TLC

  • @FlatulenceKardashian
    @FlatulenceKardashian 9 หลายเดือนก่อน

    where's Dave's hair?

  • @markmessi335
    @markmessi335 2 ปีที่แล้ว

    what is the point of the mobile fase? we have to optimize on the mobile fase in a project, but what does it do exactly?

  • @abduallah69
    @abduallah69 3 หลายเดือนก่อน

    nej tak

  • @ammarahmed3970
    @ammarahmed3970 3 ปีที่แล้ว

    you are so handsome and ur method is also smart and interesting.

  • @akimhaq1616
    @akimhaq1616 4 ปีที่แล้ว +1

    👍🏻

  • @Bludgeoned2DEATH2
    @Bludgeoned2DEATH2 5 ปีที่แล้ว +2

    TLC is so boring 😩 HPLC is way better 👍🏽

  • @Sandeepnishad90
    @Sandeepnishad90 5 ปีที่แล้ว +1

    Looking handsome

  • @hardhard8943
    @hardhard8943 2 ปีที่แล้ว

    Thank you
    Arabic

  • @Textbook000
    @Textbook000 5 ปีที่แล้ว +1

    Like like like this

  • @joslynsantana8236
    @joslynsantana8236 4 ปีที่แล้ว +1

    🙌🏽💞

  • @MarianMatejdes
    @MarianMatejdes ปีที่แล้ว

    Lets get some THC :D

  • @mrmust2780
    @mrmust2780 3 ปีที่แล้ว

    are you really professor Dave? where is your hair?