3D Printed Microscope: Hunting for Tardigrades
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- เผยแพร่เมื่อ 21 ก.ค. 2024
- 3D printing and building a microscope in the hunt for tardigrades (water bears). The cheap but powerful 3D printed microscope is the OpenFlexure delta stage: openflexure.org
Here, I used the 3D Printed delta stage, and a Raspberry pi camera v2 as optics, which is more than enough for microbes and microscopic life in pond water or moss.
The hunt for tardigrades using the 3D printed microscope started by collecting some moss from the backyard, soak it in rainwater for a few hours and then looking at in on a microscope slide.
It took me few hours but at the end, after tens and tens of rotifers, ciliates, and nematodes, I managed to find a single, beautiful, tardigrade.
00:00 Introduction
00:50 3D printing
01:15 Assembly
05:03 First tests
05:39 Microbial life in moss
08:36 Finally a tardigrade
Printed with an Ender 5 Pro in PLA Real Filament
Raspberry pi 4 (2gb) and camera v2
Music by C. Breemer (piano), bits and pieces of Nutcracker, Tchaikovsky.
www.pianosociety.com/pages/Nut... - วิทยาศาสตร์และเทคโนโลยี
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I love your selection of Tchaikovsky as much as I do the tardigrades. Bravo! ❤❤
this is so much more fun to watch because of that. planning to build an openflexure microscope myself and will make sure to listen to tchaikovsky while looking at stuff :)
Woww, for a 3D printed microscope that looks amazing!
Awesome! Love this kind of video
So nice to see this construction!
This is awesome, subbed
Vittorio in 2041: Hunting for tardigrades in deep space with my open source spaceship
Bro u are under rated
Tardigrades are so cute lmao
my least favourite part is putting the o-rings in the legs. I usually break a few rings.
if you pay close attention... I've broken one as well :)
it happened to me too. I settled for regular rubber bands I could find around
the wiggling thing was a flagellate, not a ciliate.
How do the delta stage and normal microscope compare? I am planning on building one of them, but am new to the whole topic. I'd also like to find some Tardigrades like you did, is this possible with the "beginner" microscope?
the stage is amazing with a precision of few microns movements, the microscope is as good as the objective and the camera. It really depends on you, I like the build for learning a couple of things. If you want just to look at stuff, there are cheap microscope quite good for that: th-cam.com/video/UgT52XoQGQQ/w-d-xo.html&lc=
Hi, great vidéo!
Do you think it's worth printing only the stage for use on an existing reflected light microscope?
I need to automate images captures to create very large "panoramas" at high zoom (50x objective) on my microscope, and it seems to be the cheapest solution. But on the video we can see some vibrations, and I'm not sure if it will impact the precision.
Is the repeatability good? (I need to capture tiles with fixed steps on X and Y)
it really depends on what you need it for. The stage is impressive for small controlled movements. But you may want to check as they have also only the printed stage openflexure.org/projects/blockstage/
for vibrations, all the parts are still in plastic and naturally any movement of the table will result in some degree of vibration.
you may also want to consider some cheap XY stage (from Aliexpress for example) and then using a couple of motors to make it automatic
@@v_saggiomo Thank you for the advices! I didn't know that we could find a xy stage for cheap!
Yeah the openflexure blockstage seems really cool too, but I'm not sure where I read this but it seemed to be less accurate than the delta stage. I will check again.
And it's cool if the vibrations are just an external problem. At least with a heavy table or some dampers it's possible to fix that.
Thank's again for your feedback and the video!
we've got this one few weeks ago, and it works quite well www.aliexpress.com/item/32847790758.html
but then again, it really depends on the system you're trying to build :)
@@v_saggiomo It seems cool indeed!
On my case it is to automate the acquisition of integrated circuits die shots.
Here an example of panorama taken manually with a 5x objective: i.imgur.com/gQNXCQV.jpg
It was already really long to do (~500 pictures), and now I have to take pictures with a 50x objective so you can imagine that it will be not possible by hand.
An important thing is that I need a precise step, so I can take pictures in "grid", so the panorama software will be able to process them without going crazy.
Great video! How to design your print without support?
this was not my design, but from openflexure. In general if you design without drastic overhang, it is possible to print without support. Here for example they cleverly use bridging
Wow I never found it :( on my moss
you just need a little bit of patience, rainwater, and luck. But they are everywhere. literally everywhere :)
Very nice. Which objective did you use?
it's a simple raspberry pi camera v2, with a 3D printed spacer between the lens and the sensor: build.openflexure.org/openflexure-microscope/v6.1.5/docs/#/2a_basic_optics_module
Wow I so much love this. How can I get the already assembled 3D-printed microscope please in Lagos, Nigeria 🇳🇬, I am an independent Mycology research student. Thanks
quick google search showed Stampar3D as a 3D printing service based in Nigeria, maybe contact them
DO you sell the files?
absolutely not :D
It's an open hardware project openflexure.org/projects/microscope/ you can download the files for free :)
How much did this cost
around 100euro
hyrule anybody?
ouch... the edge on your knife
made me cringe
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