ABO blood group system explained (full)

I combined my videos about ABO blood group for easy access.
Shootout to Eman Hosny @42:15 the reverse is O blood type NOT AB
Please enjoy!
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Welcome to Blood Talks Fam. Blood Talks offers you a peek into how clinical laboratory scientists (CLS) or medical laboratory technologists (MLT) work in a hospital. CLS/MLT hold an important role in the healthcare system, but unfortunately, many people do not know that they even exist, simply because most of CLS/MLT work is done behind the scenes. In this channel, you can find explanations from a clinical laboratory point of view. I hope you enjoy and learn something you did not know before.
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Disclaimer
Information provided here is for education and entertainment purposes only, not intended for diagnosis. Please consult your doctor for any health related issues you may have. As new medical technologies are updated, there are more ways to cure illnesses, new testing methods and the older methods could be obsolete. Therefore, please double check your information for the most up-to-date facts.

[@yahyaosman7364]

Perfectly understood and very nice explanation. Thank you 🙏

[@BloodTalks]

Glad it was helpful!

[@katyng29]

you explained very well which help me to understand more about the ABO blood group. Thank you very much!

[@sanjaytiwari4202]

Awesome , thx for great explanations dear madam

[@BloodTalks]

You are welcome.

[@martinconstantin500]

what are forward groups and reverse group

[@BloodTalks]

Forward is when we test the antigen on the patient's RBC. Reverse is we test for the antibody in the patient's plasma or serum.

[@draromaj]

sorry, could u tell me the solve of in forward give AB but in reverse give O ??? what I can do ??

[@BloodTalks]

No, don’t be sorry. I will try my best.
The forward gives AB and the reverse gives O.
First, check the patient’s medical history. If the patient has a history of bone marrow transplant, it could be that the patient’s blood type is changing.
Second, double check the reactions strength. The side with weaker reactions is usually the one with a discrepancy. In other words, weak reactions give you a hit as to what type of discrepancy you are dealing with.
In my experience, the forward is usually correct. The problem is more likely coming from the reverse. Let’s say that the forward reactions are 4+ and the reverse reactions are 1+.
There are a couple of things that you can do.
1. You can repeat the test using pre- warm technique. You will incubate patient’s plasma and reagent cells separately for 15-30 min. then follow your testing protocol as usual.
2. Look at the cell under microscope, you want to see if it is rouleaux or not. If you see RBC stick together in a stack like coin, then you know that the reaction is from rouleaux not real antibody-antigen reaction. You can solve rouleaux by performing saline replacement.

[@BloodTalks]

I also wanted to add that you can repeat the test with auto control and run a minicold panel.

[@shakirohakinwale3847]

Thanks for the explanation, please help in solving the following discrepancies and resolutions.
4,4,2,2
4,0,2,0
MF(4/0), MF(4/0), 0,0

[@BloodTalks]

Thank you for the question but I am not sure what are those reaction refer to.

[@sanjaytiwari4202]

Wishing you a very happy new year dear madam

[@BloodTalks]

Have a wonder years to come yourself.

[@sarahqahtani8193]

Can we gets the notes or slides

[@BloodTalks]

Thank you for your interest. This is a free resource and can be accessed on my channel anytime. I do not have this function at this time. It may be available in the future.

[@yehanantezera8219]

Do u have the notes

[@BloodTalks]

What’s note are you referring too?

[@yehanantezera8219]

@@BloodTalks about abo discrepancy. I'm not that much of visual learner that's why

[@BloodTalks]

I see. Sorry I didn’t have information written out in note formats. I will keep that in mind.

[@yehanantezera8219]

@@BloodTalks oh its ok tank u tho

[@ranakrishna2490]

What use for h1 lactin in blood bank?

[@BloodTalks]

The anti-H is made from an extract of Ulex europaeus seeds. In blood bank, we use it to detect H antigen which present in (hh) phenotype or commonly known as Bombay blood type.

[@ranakrishna2490]

What use anti a1 lactin in blood bank?

[@ranakrishna2490]

Forword group A+ve & reverse blood group O+ ve how to confirm patient blood group?

[@BloodTalks]

@@ranakrishna2490 The antisera A1 lectin tests antigen on the patient’s RBC. You will mix patient’s RBC with antisera A1 lectin, centrifuge, and read the reaction. A1 subgroup patient will react with anti-A1 lectin.

[@BloodTalks]

@@ranakrishna2490
Forward A pos reverse O
This usually a discrepancy in the reverse type.
Given the following reaction:
Anti-A = 4+
Anti-B = 0
A1 cell = 1+
B cell = 4+
Looking at this reaction there are a few possibilities
1. Cold antibody - prewarm method
2. A subgroup - run Anti-A1 lectin. If the patient is A1 negative repeat the test using A2 cell. If you do not have A2 cell, search for A1 negative donor unit and use that for testing.
Note A1 is the most common subgroup of A blood type