Making a 3-5% RBC Suspension
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- เผยแพร่เมื่อ 21 ส.ค. 2024
- PROPERTY OF THE INDIAN IMMUNOHEMATOLOGY INITIATIVE
www.indianinitiative.org
Susan Johnson, MSTM, MT(ASCP)SBB, MS, Associate Director of the Indian Immunohematology Initiative, demonstrates how to prepare a 3-5% red blood cell suspension that can be used in hemagglutination testing to determine blood group.
www.indianinitiative.org
Доступно с русскими субтитрами! Приготовление 3-5% клеточной суспензии.
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another method wash cells 3x, then on other tube put for example 500 microliters of saline then computed 5% is 500 x .05 where 500 is saline and .05 is 5% pure washed red cells so 25 microliters =5% red cell suspension.
Super helpful, thank you!
Thank you very much!! That's such a good tip to compare your suspension to the reagent cells! Brilliant! Haha
All of my classmates will be elated when I relay this technique to them.
Excellent. Very helpful.
I usually like to compare the suspension to the color of fruit punch. I have my lab practical tomorrow and this was a very helpful video.
Thank you very much
many thanks ...
excellent
im sorry i didn't understand well but why is there are 3 pairs of tube?
Green Iris I believe there are a total of 3 different concentrations (just right, too heavy, and too light). Each suspension was tested with both anti-A (blue reagent) and anti-B (yellow reagent).
If this was a real patient workup, there would only be 2 tubes for the forward type.
it is for demonstration only to know what will appear if we use too diluted or too concentrated RBCs suspension
hello i have a questions
1. Why need to have 5% cell suspension?
2. What is the purpose
3. Different between use whole blood and 5% cells suspension
what if commercially prepared cell suspension is not available to compare with?
you need you buy one, then you can make one to keep it available for the next suspension
why is it called 3-5%??