Spirostomum - Nuclear division of the macronucleus
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- เผยแพร่เมื่อ 7 ก.พ. 2025
- Spirostomum sp. is on the longest ciliates and it contains a uniquely shaped macronucleus - often times reffered to as "beads on a string" like. The "beads" are called nodules and a single individual contains 10-50 nodules. The number of nodules positively correlates with age - the older the ciliate the more nodules it has.
This video shows a nuclear division of one of the nodules. In the video, the ciliate contains 16 nodules with one of the nodules undergoing nuclear division. After the division, the ciliate will contain 17 nodules - which means this individual is rather young.
The video was made on a Olmypus Vanox AHBT3 in DIC with the Olympus SPlanApo 20x/0.7. The video was filmed with a Panassonic S1 that was adapted using an NFK 2.5x projection eyepiece.
Wow. The images from that Olmypus Vanox are incredible!
I'm glad you like the video! Was a long road until I achieved such image quality
Very cool, and captured through one of my favorite objectives!
Also my favourie objective! I always have a "WOW" effect when using it. No other objectives comes close to this bad boy
@@VANOXmicroscopy I recently tried to buy the exceedingly rare 60x 1.40 off a guy on ebay but he wanted way too much for it. I have a feeling that one might be up there although I don’t know if it would play nicely with any of the DIC prisms.
@@diettoms The 60/1.4 is very rare indeed. Took me years until I found one that was reasonably priced. Got one last year for 350$ dollars.
There was a special DIC prism for the 60/1.4 objective from Olympus (but only for the universal condensor for the BH-2 series, they didn't have one for the Vanox series for whatever reason). In my case, the objective works ok (not perfect) with the DIC prism of the 40x objective (I have the DIC prisms which were originally intended for the DPlanApo UV series). You won't be able to extrapolate my experience with the DIC of the 60x to your scope though, condensor and everything is completely different.
Another point is, although the objective is usable in DIC, I'm not really benefiting from its high resolution because the DIC prisms was intended for the DPlanApo UV 40/0.85. That means the maximum aperture I get wih this prism is 0.85 anway and not the maximum 1.4 which this objective can deliver. That's why I'm mostly using it for epi-fluorescence where this high aperture is really good. Instead of the 60x objective for DIC, I mostly use the Leitz NPL Fluotar 50/1,0 (excellent piece of glas).
@@VANOXmicroscopy Thank you so much for these tremendous insights! I bet that 60x prism is a real pain to track down too. I have the old school "NIC" condenser with all the prisms locked in place anyway.
350 is absolute robbery - I ought to call the police! If you ever do decide that you're not getting enough use out of it, let me know, I would gladly pay more. In the meantime, I did pick up a Nikon 60x 1.4 as I am starting to bring my Labophot 2 system together. I've heard good things about it - hopefully it's in prime shape when it arrives!
@@diettoms Ahhhh okay the NIC version..Don't know much about this one. What front lense does the condensor have ?
In case I will not need it anymore I will let you know. But that will probably only be the case after the unlikely event of me switching to infinity scopes one day ;) But who knows ^^
Let me know how the Nikon 60x performs on your Olympus scope!!!
I also make videos of microscopic beings.
Very cool, just subscribed :)
Such a really cool specimen. How where you able to get such a clean, clear, sharp image? Is there a special microscope you had to use? Or objective lens?
Glad you like the image quality :)
Achieving this clarity is actually a combination of many parameters: First of all I have a fairly good microscope from Olympus. Although it is more than 30 years old, it was once used for actual biology research at a universities. I equipped the microscope with pretty good objectives over the time (SPlanApos from Olympus), attached a modern full frame camera to the microscope (Panasonic S1) and I use differential interference contrast (DIC) for pond life organisms. The DIC is giving excellent contrast and resolution. Last but not least, a lot of experience...Preparing these slides is not an easy thing to do as these high aperture objectives are really sensitive and everything needs to be set up perfectly. Hope that helps a bit, if you have more questions, just let me know :)
@@VANOXmicroscopy I have an Omax microscope that’s roughly $240 in price, which is sorta cheap in this hubby I’m guessing, but I wanted to try and get better, more detailed/sharp, imagery out of it. Is there any particular objective lenses that could achieve this? Any lenses you could recommend? And does it even make sense to try and upgrade a beginner microscope? Seeing that I could later on try and buy a nice vintage Olympus microscope.
I’m definitely loving this hubby and exploring the microbes that’s in my local ponds. I’m definitely loving these types of videos as well.
@@BermyNick I think you could upgrade your Omax a bit with "new" lenses from the used market. For example objectives from Leitz, Zeiss, Nikon or Olympus. They all have the same thread size called RMS so you can swap them. What you need to keep in mind though is, that objectives from a certain brand need the corresponding eyepieces from that particular brand. So if you buy Olympus objectives you need to use WHK eyepieces from Olympus to get optimal image quality. Mixing here is not a good option.
You probably won't be able to upgrade the Omax with other contrasting techniques like phase contrast or DIC but you can use the objectives you aquire in the meantime for the microscope you may be purchasing in the future.
I'm glad your enjoying this hobby as much as I do :)
Do you do polarized microscopy? If so can you share your setup!
Yes I also do polarization microscopy!
For example you can check out this video where I looked at Vitamin C:
th-cam.com/video/k_rqCsxWj3k/w-d-xo.html
I'm doing my videos on a Olympus Vanox AHBT3 using the original Olympus Polarizer and Analyzer.
If you need any details, just let me know :)
Do you sell NFTs?