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VANOXmicroscopy
Germany
เข้าร่วมเมื่อ 26 ธ.ค. 2020
I am a molecular biologist and a passionate microscopist for almost a decade. I love sharing my microscopic findings and try to deliver microscopic images with high resolution and quality.
This channel is not only about findings under the microscope but will also cover maintance of vintage microsopes and technical aspects of microscopy.
The main microscope I'm using for my videos is an old Olympus Vanox AHBT3 with Brightfield, Phase contrast, DIC, Polarization and Epi-fluorescence.
I hope you enjoy my videos and feel free to contact me any time!
VANOXmicroscopy
This channel is not only about findings under the microscope but will also cover maintance of vintage microsopes and technical aspects of microscopy.
The main microscope I'm using for my videos is an old Olympus Vanox AHBT3 with Brightfield, Phase contrast, DIC, Polarization and Epi-fluorescence.
I hope you enjoy my videos and feel free to contact me any time!
VANOXmicroscopy
Tick Expedition: Journey from Field to Microscope
Ticks are ectoparasites that feed on the blood of vertebrate hosts. They have specialized mouthparts for piercing the skin and can transmit diseases such as Lyme disease. Ticks go through a four-stage life cycle, requiring blood meals during each stage. They are commonly found in grassy and wooded areas and use sensory structures to detect potential hosts. In this video I will show you how to catch a huge numer of ticks in the field to prepare slides for microscopy using a simple white bedsheet.
Microscope:
Olympus Vanox AHBT3 with brightfield & DIC
Objectives used:
Olympus UVFL 10/0.4
Olympus SPlanApo 20/0.7
The camera is a Panasonic S1 adapted with an Olympus NFK 2.5x eyepiece.
Sources used in this video:
- www.inspq.qc.ca/en/detailed-description-ixodes-scapularis-or-black-legged-tick-or-deer-tick
- parasitology.cvm.ncsu.edu/vmp930/keys/ticks/tickterms.html
- en.wikipedia.org/wiki/Tick
Microscope:
Olympus Vanox AHBT3 with brightfield & DIC
Objectives used:
Olympus UVFL 10/0.4
Olympus SPlanApo 20/0.7
The camera is a Panasonic S1 adapted with an Olympus NFK 2.5x eyepiece.
Sources used in this video:
- www.inspq.qc.ca/en/detailed-description-ixodes-scapularis-or-black-legged-tick-or-deer-tick
- parasitology.cvm.ncsu.edu/vmp930/keys/ticks/tickterms.html
- en.wikipedia.org/wiki/Tick
มุมมอง: 496
วีดีโอ
Feeding Paramecium with stained yeast
มุมมอง 3.6Kปีที่แล้ว
Paramecium caudatum is a well known ciliate and model organism in biology . Its comparatively large size make it easy to observe its internal features. This video shows the digestion process in Paramecium using bakers yeast that was stained with congo red - a pH indicator. A small amount of fresh yeast is dissolved in water and the solution is brought to a boil to disrupt the cell wall of the y...
Observing mitochondria under the microscope | Fluorescence staining of onion cells
มุมมอง 2.2K2 ปีที่แล้ว
Cytoplasmic streaming can easily be observed in epidermal onion cells. Preparing microscopic slides from onion cells is fairly easy and is therefore one of the most studied specimen in schools. With proper illumination techniques, it is possible to not only observe the very prominent cell nucleus but also many other organelles inside the cell including Leucoplasts, Spherosomes and Mitochondria....
Cytoplasmic streaming in Paramecium bursaria
มุมมอง 4382 ปีที่แล้ว
Paramecium bursaria is an ideal organism to observe cytoplasmic streaming. Cytoplasmic streaming describes the flow of cytoplasm and all its constituents inside the cell. This process is mediated by the cytoskeleton and motor proteins which work hand in hand to form a highly dynamic structure that enhances the speed of distribution of cellular organells, macromolecules and solutes. This increas...
Paramecium bursaria - Symbiotic algae avoiding phototoxicity
มุมมอง 6072 ปีที่แล้ว
Paramecium bursaria is the only Paramecium species hat is living in symbiosis with algae from the genus Zoochlorella. The algae supply the Ciliate with food while Paramecium provides a protective environment. The Algea convert sunlight to carbohydrates and energy using photochemsitry. As strong light light can be harmful to both the algea and the host, excess excitation energy is either dissipa...
Zeiss microscope nosepiece maintenance
มุมมอง 1.4K2 ปีที่แล้ว
This video shows the maintenance procedure for a vintage Zeiss Standard microscope nosepiece (revolving nosepiece/turret/revolver). The particular nosepiece in the video is from a Zeiss Standard Photomicroscope I but Zeiss Standard nosepieces are build very similarly. We start by unscrewing the first screw (left-hand thread!) and remove the special bolt underneath it using a wide flat head scre...
Paramecium - Contractile vacuole at 1250x
มุมมอง 1.8K3 ปีที่แล้ว
Paramecium caudatum is a well known ciliate and model organism in biology . Its comparatively large size make it easy to observe its internal features. This video shows the Macronucleus, Cytostome, Caudal Cilia, Food vacuoles, Contractile vacuoles and Trichocysts at up to 1250x magnification in differential interference contrast (DIC). Microscope: Olympus Vanox AHBT3 with DIC Objectives used: L...
Oligochaeta anatomy under a microscope - Pristina sp. (DIC)
มุมมอง 1.3K3 ปีที่แล้ว
Earthworms are a typical model organism in biology and are often used to demonstrate internal organs and to practice the dissection of an animal. However, dealing with dead animals is honestly not that exciting. Microscopic freshwater worms can be great models to study the internal organs and their functioning in a living animal because of their transparent body. In this video, the Oligochaeta ...
Spirostomum - Nuclear division of the macronucleus
มุมมอง 7043 ปีที่แล้ว
Spirostomum sp. is on the longest ciliates and it contains a uniquely shaped macronucleus - often times reffered to as "beads on a string" like. The "beads" are called nodules and a single individual contains 10-50 nodules. The number of nodules positively correlates with age - the older the ciliate the more nodules it has. This video shows a nuclear division of one of the nodules. In the video...
Phagocytosis of Parkinson proteins in THP-1 cells using fluorescent microscopy
มุมมอง 4123 ปีที่แล้ว
Phagocytosis assays are a common tool in pharmaceutical research to investigate the behavior of human cells upon treatment with antibodies or chemical modulators. In this video, we will look at the phagocytosis of pHrodo labeled alpha-Synuclein proteins. This protein is a hallmark of Parkinson's disease, the second most common neurodegenerative disorder. Improving the phagocytic capacity of bra...
Killing a Ciliate with light - phototoxicty (fluorescence microscopy)
มุมมอง 5K3 ปีที่แล้ว
A short clip that shows the effect of strong excitation light on living organisms during fluorescent observations. This Ciliate was stained with acridin orange (AO) and too strong excitation light (blue light) was used. The Ciliate quickly disintegrates by loosing pieces of its membrane and it eventually explodes. The phenomenon of damaging cells during fluorescent observations is called photot...
Staining Ciliates for fluorescent microscopy
มุมมอง 1.9K3 ปีที่แล้ว
In this video I show the procedure of staining ciliates with acridin orange (AO) for fluorescent microscopy. Acridin orange (AO) is a common fluorescent dye that stains DNA and also accumulates in acidic compartments. It's cell permeable and therefore allows live-cell imaging of the ciliates. The wavelength of the emitted light is dependent on the pH. It is therefore possible to observe the sta...
Feeding a microscopic monster (Hydra under the microscope)
มุมมอง 11K3 ปีที่แล้ว
Feeding Hydra vulgaris with brine shrimp (artemia) under the microscope in darkfield. Hydra vulgaris was placed on a microscopic slide from a culture and then a small amount of brine shrimp culture was added. The hydras were allowed to sit on the slide for 10min to adjust to the new environment. Most of the footage is timelapsed 20x times (especially the ingestion process) The footage was captu...
Tardigrade up close and personal under the microscope (DIC)
มุมมอง 1K3 ปีที่แล้ว
Getting really close to a Tardigrade which are also known as little water bears. These microscopic animals can often be found in moss samples which is a harsh environment to live in: These biotopes frequently alternate between high humidity and absolute dryness, that means that animals that want to survive have to be exteremely well adapted. The Tardigrade is one of them. They can surive in ext...
Vitamin C crystals growing under the microscope (Polarized light)
มุมมอง 3.5K3 ปีที่แล้ว
Vitamin C crystals growing under the microscope (Polarized light)
Fungi infected Oligochaete - Pristina sp. under the microscope (DIC)
มุมมอง 3.3K3 ปีที่แล้ว
Fungi infected Oligochaete - Pristina sp. under the microscope (DIC)
Amoeba from hay infusion - Contractile vacuole in action (DIC)
มุมมอง 2K4 ปีที่แล้ว
Amoeba from hay infusion - Contractile vacuole in action (DIC)
Tartaric crystals growing under a microscope (Polarized light)
มุมมอง 6334 ปีที่แล้ว
Tartaric crystals growing under a microscope (Polarized light)
Amoeba timelaps in differential interference contrast (DIC)
มุมมอง 2944 ปีที่แล้ว
Amoeba timelaps in differential interference contrast (DIC)
Spirostomum ambiguum - Giant Ciliate under a Microscope (DIC)
มุมมอง 6804 ปีที่แล้ว
Spirostomum ambiguum - Giant Ciliate under a Microscope (DIC)
Gorgeous footage!! Does your vanox have the 20x prism or were you able to use the 40x prism for the 20x objective?
Cheer~~~a space or vesicle within the cytoplasm of a cell, enclosed by a membrane and typically containing fluid.😊
Amazing video
fluorescent microscopy does not convince me, also the super care objctives that are needed i dont wnat to spen hundrets of bugs for a 40x oil with NA of 1.3
staining nukleus with haematoxylin works better and is much cheaper
@@lotharmayring6063 True, it's much cheaper but unfortunately it does not work for live cell imaging
Dear @VANOXmicroscopy, I would like to invite you to join a live session with MicrobeHunter . He is planning to do a "SPECIAL live session" and include other amateur microscopist to join the live with him in 2024 and/or beginning 2025. It would be such an honour to have you on the live with Oliver I invite you to contact him if you are interested (you can find his e-mail on his website). There is no official date yet, It could be in few weeks and maybe more than once if it's a success. No pressure, just 3-4 peoples with microscope and camera sharing various live observation. Seriously.... pleeeeeeeeeeeeeease consider it! I'm just a regular subscriber of MicrobeHunter's channel. And I just subscribed to yours today because of your amazing work.
You are probably very busy in your life and microscopy is a very niche topic on TH-cam... But I hope one day you will have time to make another of theses masterpiece!
I read all of your comments and I just wanted to thank you very much for all the kind words! :) I sure hope I will have more time in the future to continue.
@@VANOXmicroscopy I'm glad you read my comments; you deserved theses compliments! And you can make a video each 2 years or never, it's up to you. We will be in standby mode, like a well behave tardigrade.
I very appreciate all the info in your description. Very useful indeed! That is perfect science on TH-cam, thank you for your time.
Ok.. I just look at one of your objective.. used... 4800$ Your image is just INCREDIBLE!!! If I was a flatearther, I would say it's CGI! I would love to see a direct comparison between your Olympus Vanox AHBT3 Vs my cheap SWIFT380 microscope? (or comparable cheap microscope) With the same camera and same slide and same magnification.
I would suggest you to show a small image of you doing the preparation. It's always nice to have visual support. What a great image quality you have by the way!
We have a lot of flatearther video, flatearther debunk video... Meanwhile... your video... I very appreciate you share this info with use. Even if you got 'only' 378 views in 2 years (2024). Very interesting... and hopefully, it will get somebody involve with the amateur microscopy hobby or to study in this field of research.
What a great idea!!!
Where can we buy such AO if we are not a company? And what would be the typical price for a very small quantity? Living in Canada
For amateur microscopist on a budget To have fun with microscopy fluorescence on a very limited budget, you can use any cheap microscope and highlighter ink as poor's man die. Just put the tip of the highlighter on a drop of water (yellow, orange, green, pink, etc fluo pen). Take some of this diluted die and mix it with your sample that contain protozoa/paramecium/ciliate/bacteria. Use a 3$ UV/purple 405nm flashlight to look the amazing glowing of living organisms!
Fascinating, I look forward to your future videos!
What a fantastic little video, that no doubt must have required a lot of work on your end. I especially appreciate the combination of clear, educational commentary with instructions to reproduce the experiment, alongside the beautiful footage. I will save this video to try and reproduce with our young ones. Thank you
Podrías hacer una reseña de tu microscopio Olympus?
amazing footage, thank you so much
VANOXmicroscopy, you are very generous in sharing with us your method for maintaining a Zeiss microscope revolver. I have aZeiss microscope Standard 14 and I think I will follow your very detailed advice. I hope the "lens key" is easy to find.
My objective is too big 🎉🥶🥰
MAGNIficent🍆🍑🌮😛
Nice man 40x is where it starts🎉
Amazing!! Are you mixing the tartaric acid with water?
Yes it is really as simple as that. I make a saturated solution of tartaric acid in water, put a drop on a slide and heat it under a flame until evaporation starts occurring. I then place the slide under the microscopy quickly and start observing. The process I just described is briefly shown in my Vitamin C crystals video in case you want to see it :)
Thank you! @@VANOXmicroscopy
What's this?
A very big unicellular organism :)
Another great video, I am interested in your post processing regime. Do you do color grading? What software do you use?
Which Paramecium species is this? Thank you.
It is most likely paramecium caudautum!
Thank you.
fluoescenz is still unsharp. I get better results with normal selectiv dies . Makes no sense for hobbyists. DIC is still much better
For some amateur microscopist, the cool factor is way more important compare to the purely scientific value of such technique. When I was working in analytical lab, the cool factor was only a side benefit, not the same purpose here. Also, DIC equiped microscope are way more expensive compare to basic DIY fluorescence microscopy that can be made with cheap scope such as SWIFT350 and a 3$ purple flashlight.
@@alexandrevaliquette3883 i made a DIY DIC buying 2 Wollastones for 5O bugs. Ag good epifluorsececes need much more money for the illumination tube, dichroitic mirror, the 2 fiters and the UV source. All other DIY epi fluorsecense i have seen here are bullshit and waistmoney
Amazing!!! So those were ALL Different Vitamin C Crystals??
Thank you :) Yes they always crystalize randomly resulting in all these different patterns. That's why you can repeat this experiment over and over again and always see something new!
Wow, can you show what the DIC setup looks like? I have a similar microscope but not sure how to set it up.
Sir... I would love to see a video just dedicates to the microscope setup you use. The Vanox AHBT3 must be a BEAST.
Thanks for this video. I am adding a florescence system to my Nikon Diaphot. I purchased the AO powder and measuring tools you mentioned. I'm completely new to florescence, and tips or resources would be greatly appreciated.
I think you already got a lot of input over at Discord if I'm not mistaken? Otherwise I can check if I find some additional useful resources for you :)
@@VANOXmicroscopy yes I did, thanks.
CATCH LYME DISEASE AND YOU'RE SCREWED
That's why I always check myself after my field trips :)
@@VANOXmicroscopy TAKES JUST ONE TO CRAWL UP AND BITE YOU AND BINGO
@@leelunk8235 You are right but for me that's not a reason to not go outside. Every forest, every field where I live is full of ticks. You would have to lock yourself in to be safe. The only thing you can do Is try to minimize the risk by wearing long trousers, long socks, insect repellent, checking yourself after you have been in nature and if you got bitten then closely monitor the site you got bitten.
@@VANOXmicroscopy YEA TRUE, MITIGATE THE RISK
Wonderful, thanks for the tick anatomy lesson.
Thank you!!!
Great video and beautiful pictures of such creepy creatures. Thanks for that #sexiestscientistalive 🔥
Muito obrigada, você me ajudou muito!!!!! Eu sou estudante de ciências biológicas, vou fazer um seminário sobre as mitocôndrias e, não havia encontrado nenhuma imagem microscópica das mitocôndrias.
Back again! Always nice to see you upload, great footage and info per usual :)
Thank you Brian!
He returns !! good stuff my guy!
Thank you :D Happy to see that my trusty subscribers are still here :)
@@VANOXmicroscopy Im here as long as you are lol.
@@SLAUGHTEREDEYES <3
Welcome back! He is alivee!😀
Haha of course :D
Amazing video, you deserve way more subscribers
Crystalized sunlight
Have you ever been able to catch Hydra splitting into two?
Im very impressed not only by the footage but also all the work and information you give us. Thank you very much :)
Thank you so much for the kind comment. Much appreciated :)
Awesome
You can do it using mercury lamp, Most microscopes have shutters for it... No need to power lamp off and on...
You are right! Didn't think of that 👍
Excellent VANOX! I made a mitochondria video myself (th-cam.com/video/Rz5W2KCGeVM/w-d-xo.html). Yours is better!
Great! I should also try Janus Green.
Bravo David!