DNA ligase
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- เผยแพร่เมื่อ 29 ส.ค. 2024
- This lecture about DNA ligase explains how does DNA ligase works. shomusbiology.com/
Download the study materials here-
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In molecular biology, DNA ligase is a specific type of enzyme, a ligase, (EC 6.5.1.1) that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV) may specifically repair double-strand breaks (i.e. a break in both complementary strands of DNA). Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template,[1] with DNA ligase creating the final phosphodiester bond to fully repair the DNA.
DNA ligase has applications in both DNA repair and DNA replication (see Mammalian ligases). In addition, DNA ligase has extensive use in molecular biology laboratories for recombinant DNA experiments (see Applications in molecular biology research). Purified DNA ligase is used in gene cloning to join DNA molecules together to form recombinant DNA.
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Very good explanation about the difference between "nick" and "gap". Thank you very much!
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In book I find difficult to learn .You explained well .May you live long and keep up with good work
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Zazakhallah khairan.
Thanks for your thorough explanations. Im a 4th year at UCSD taking a biochem class called Biosynthesis of Macromolecules and your videos are helping me a lot! Just came across this and it was really helpful. Im about to check out all your biochem videos. Thanks for posting these
sir i am a student of 12th class and today i had a presentation on DNA polymerase 3 and dna ligase. i saw your videos last evening i got the concepts very clear .My professor got stunned ..The best day of my life just because of you...i love you sir.
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Thank you so much sir... I'm lost for words to describe your fantastic teaching skills and depth of knowledge. Your videos provide deeper and clearer understanding of concepts.
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You have explained deeply and in detail and understandably😊
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thanks for every thing i m following u from my bsc 1st year...now i m persuing msc......ur lactures r really helpful....plz a live session
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very well explained :) cleared all doubts....god bless you sir :)
+chainika gupta thank you.
Your give your all with full hard working I hope I will watch all of your genetics related videos
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Awesome sir ji.
Your videos are always simple and easy to understand.
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I couldn't locate a video that explained this subject better.
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very simple and interesting way to describe.
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whenever I don't understand something , I head to youtube find your videos
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It's very helpful video sir
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Sir please make a video on homopolymer tailing technique.....as soon as possible you are the best sir God bless you always
Okay
sir can you give the study material for dna ligase mechanism...also it would be really helpful in other videos too if you provide study materials for the topic :) tysm your videos help me alot
Please make a video of polynucleotide kinase and alkaline phosphatase... Asap... Please.....
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Very good explanation thank you so much sir
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Very well explained.
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amazing explaination Sir
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Thank you so much sir
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What is the other ways of joining DNA molecules accept dna ligase..?
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sir its was an beautifully illustrated explanation , but I have a doubt. How does the process of unwanted self ligation of a cleaved plasmid vector in rDNA technology, take place, without the interference of ligase enzyme?
+Sai Sarada Mythili Kothapalli thank you
Wow thanks that's a clear explanation
please upload a video on DNA purification
Can you share the detail mechanism video here
Thank you a lot your video,
Thank's it is very useful
Thanks sir, it was helpful
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Why does a phosphorus molecule need to be emitted out for a phosphodiester bond to form?
Awesome explanation👍
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thanks alot very nice lectures...have u these lectures in notes form pllllzzzz tell me..i have some prblms so cannot see your every video lecture...plx i need it in written form
+zoni Zoni thank you. Glad you liked my lectures
Can someone tell me why there would only be 1x Phosphate left on the 5' end after Okazaki Formation? Something is stuck in my head.. thx
great video my dude
Sir your videos are great.
My book there is a question to write about the formation of DNA ligase what should be the answer please reply
Zazakallah khairan.
well done buddy thanks
sir i need the clear difference between polynucleotidal kinase and dna ligase pls sir can u clear my doubt
sir how will i get all molecular biology videos in sequence?? its quite confusing
Oooooooooooooooooohhhhhhhhhhhhhhh now i get it!
Nice videos keep it up
thank you for this video! anyway has that enzyme atp complex Quatern structure? :)
Sir please make a video on dna modifying enzyme kinase please please
please tell poly nucleotide kinases
Sir pls give the name of the phosphate you are saying at 11:37
Sir, Which book to follow for Molecular biology.
When there's a gap, how are nucleotides added?
+Amna Zaim with normal DNA polymerization process with polymerase enzyme
Hello Sir.....I want you guidance.... Could you please help...me🙏
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Explain in hindi
Y?
So lag. Bad. Not interesting
Can you share the detail mechanism video here
Okay