DNA ligase

This lecture about DNA ligase explains how does DNA ligase works. shomusbiology.com/
Download the study materials here-
shomusbiology.w...
In molecular biology, DNA ligase is a specific type of enzyme, a ligase, (EC 6.5.1.1) that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV) may specifically repair double-strand breaks (i.e. a break in both complementary strands of DNA). Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template,[1] with DNA ligase creating the final phosphodiester bond to fully repair the DNA.
DNA ligase has applications in both DNA repair and DNA replication (see Mammalian ligases). In addition, DNA ligase has extensive use in molecular biology laboratories for recombinant DNA experiments (see Applications in molecular biology research). Purified DNA ligase is used in gene cloning to join DNA molecules together to form recombinant DNA.

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Okay

[@snehamazumder2365]

sir can you give the study material for dna ligase mechanism...also it would be really helpful in other videos too if you provide study materials for the topic :) tysm your videos help me alot

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Please make a video of polynucleotide kinase and alkaline phosphatase... Asap... Please.....

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Very well explained.

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amazing explaination Sir

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[@bhattigaurav9411]

What is the other ways of joining DNA molecules accept dna ligase..?

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[@kssmythili982]

sir its was an beautifully illustrated explanation , but I have a doubt. How does the process of unwanted self ligation of a cleaved plasmid vector in rDNA technology, take place, without the interference of ligase enzyme?

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Wow thanks that's a clear explanation

[@mami4037]

please upload a video on DNA purification

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Can you share the detail mechanism video here

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Why does a phosphorus molecule need to be emitted out for a phosphodiester bond to form?

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[@cheeamayne]

Can someone tell me why there would only be 1x Phosphate left on the 5' end after Okazaki Formation? Something is stuck in my head.. thx

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Sir your videos are great.
My book there is a question to write about the formation of DNA ligase what should be the answer please reply

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[@geethakiran4208]

sir i need the clear difference between polynucleotidal kinase and dna ligase pls sir can u clear my doubt

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thank you for this video! anyway has that enzyme atp complex Quatern structure? :)

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Sir please make a video on dna modifying enzyme kinase please please

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please tell poly nucleotide kinases

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Sir pls give the name of the phosphate you are saying at 11:37

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When there's a gap, how are nucleotides added?

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+Amna Zaim with normal DNA polymerization process with polymerase enzyme

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Can you share the detail mechanism video here

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Okay