Inoculating with liquid cultures and utilizing media extraction techniques for mycology

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  • เผยแพร่เมื่อ 15 มิ.ย. 2024
  • This video is about Inoculating liquid cultures and utilizing media extraction techniques - The media extraction device was produced by Lion's Mane in Denver, CO. Check out their website for more information lionsmanedenver.com For more information about our business visit freshfromthefarmfungi.com and to purchase our cultures visit our ETSY shop FRESH FUNGI www.etsy.com/shop/freshfungi MUSHLOVE
    Gary's Recommended Products: www.amazon.com/shop/influence...
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ความคิดเห็น • 271

  • @davidcummings4273
    @davidcummings4273 4 ปีที่แล้ว +2

    Wow, nicely done. Very informative and helpful. Thank you for making them.

  • @christianlander321
    @christianlander321 ปีที่แล้ว +1

    Really informative video I learned some key things that I will be doing from now on with my LC it's very easy to pick up what you are explaining I like how detailed your videos are. Will be hanging out watching for the next one after I subscribe. Thanks Gary

  • @karloskardenas2414
    @karloskardenas2414 2 ปีที่แล้ว

    Thanks for the info and your time!!!🙏🏻

  • @DigbyGrayston
    @DigbyGrayston ปีที่แล้ว

    loving yr show and info ... keep it up ...learning so much from Australia

  • @neogeo8267
    @neogeo8267 4 ปีที่แล้ว +8

    Gary, I really appreciate the QC / Test portion of this video - I need a lot more of this sort of thing for our process. We're learning the hard way as we go and your videos are super helpful - thank you! Where are you getting your QC plates and other lab supplies if you don't mind sharing? Thanks again! George in Idaho

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  4 ปีที่แล้ว +7

      Neo Geo Thanks for watching we appreciate you’re support! We get our QC plates through Hardy Diagnostics and our lab supplies we source all over depending on our needs : ) Hardy Diagnostics and Thermoscientific are good brands

  • @user-fj8xs7yj9d
    @user-fj8xs7yj9d 11 หลายเดือนก่อน

    i appreciate you making the distinction between aseptic and sterile, huge pet peeve of mine

  • @tsymnz66
    @tsymnz66 3 ปีที่แล้ว

    I bought some Turkey Tail from you, amazed how fast it grew on agar plates! Highly recommend this grower guys.

  • @love2grow493
    @love2grow493 3 ปีที่แล้ว +1

    Vert nice I would just use the LC jar and pore it in to my grain but would be nice if you make a lot of LC syringe to make.

  • @TheMyco-Psycho
    @TheMyco-Psycho 6 หลายเดือนก่อน +1

    Greetings Gary, your videos & the information you provide are some of the very best. Thank you for your commitment to excellence and your willingness to share some of your hard-won wisdom with the rest of us! I wanted to ask you about your blood agar and the hektoen enteric agar plates you utilized in this video. Do you make your own, or do you purchase those plates pre-made from a supplier? I would sure love to see you do a comprehensive video on quality control strategies... thanks in advance for your response, Merry Christmas to all of you out there in Colorado.

  • @vincentwu2848
    @vincentwu2848 ปีที่แล้ว

    Hi Gary! Thanks for another awesome video, thanks for sharing! I couldn't help but notice the cracks in the walls of your lovely laminar flow hood. Is there an interesting story behind that, perhaps one that involves a pressurized vessel unexpectedly coming apart?

  • @andrewstark4798
    @andrewstark4798 3 ปีที่แล้ว +2

    tanks for the vids man! you inspired me to make a lab and start a mushroom farm!! mush love fam

  • @kicknadeadcat
    @kicknadeadcat 3 ปีที่แล้ว

    I started straining the lme in a coffee filter from the water after it’s all mixed up. Then pc it. Crystal clear.

  • @michaelsanchez8457
    @michaelsanchez8457 3 ปีที่แล้ว

    I use a three way valve in some ozone therapy. It's probably not the best solution, but it would be straightforward to have a line in to the valve, and the other two spots connected to the syringe and the needle, in a linear fashion.

  • @shabamodest8202
    @shabamodest8202 ปีที่แล้ว

    Hey Gary I know this is an old video, super appreciate your video been following along for years. My question would be do you have any advice for using these media extraction lids in a SAB environment? Especially as far as keeping the luer lock sterile

  • @theruralgardener123
    @theruralgardener123 2 ปีที่แล้ว

    Hey Gary! Once again, another super video. I am all about QC, that being said, would you share which of the blood agar plates you purchase from Hardy? There are a lot of them. Also the one you use specifically for salmonella and coli?

  • @user-cx5mq6nk1b
    @user-cx5mq6nk1b 4 ปีที่แล้ว +2

    I work with almost the same setup, but I insert an sterilized automatic syringue in the big jar through a silicon tube and a needle. The grain jars have an injection port, so I can inoculate hundreds of jars in minutes without opening them, saving time and possible contamination. Also, I seal the bags before sterilization, and do the same as before through an injection port.

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  4 ปีที่แล้ว

      石头 awesome that sounds like a great and efficient setup

    • @user-cx5mq6nk1b
      @user-cx5mq6nk1b 4 ปีที่แล้ว +1

      @@FreshfromtheFarmFungi Also, I'm working with that setup to inoculate logs in the forest in a most sterile and fast way than the traditional, with only a battery drill and a peen hammer. With the help of two friends, one drills narrow but deep holes in the log or tree, another insert the syringue and inoculats it, and the third one only needs to close the hole with the peen hammer, like when you make a rivet. This way, there is no need to drill big holes and fill them with spawn, or using wax or stoppers to seal it. But I'm still not sure about it's viability.

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  4 ปีที่แล้ว +1

      石头 would love to see some of the results! MUSHLOVE

    • @cooleve
      @cooleve 2 ปีที่แล้ว

      can u post some videos

  • @glennplatvoet7111
    @glennplatvoet7111 4 หลายเดือนก่อน

    I like the new setup have done lab professional hope morels take thats 👍👍👍 thanks for the video

  • @j_larusta_8825
    @j_larusta_8825 3 ปีที่แล้ว +1

    thank you for this video

  • @charliebande3845
    @charliebande3845 3 ปีที่แล้ว

    Very nice, thank you.

  • @brianagiraldo-frendo7442
    @brianagiraldo-frendo7442 3 ปีที่แล้ว +1

    Hey! Love this video, the media extractor, and your technique. Are you using a vertical hood? Are there specific qualities I should look for in a honey? What size test tubes do you use for your slants? What type of tissue culture/solution was in the vial you used to inoculate the honey LC and how big was it?

  • @st3am941
    @st3am941 2 ปีที่แล้ว +1

    This reminds me of my work in the OR. Wouldnt hurt to wipe the tube tip before placing the syringe with alcohol wipe to avoid any contaminates on the tip of the tube.

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 ปีที่แล้ว

      yes similar aseptic techniques - I agree that could be a great extra precaution

  • @bradford.champlin
    @bradford.champlin 4 ปีที่แล้ว +4

    Well done. Really interested in the pnematic pump idea

  • @kodyfrost
    @kodyfrost 4 ปีที่แล้ว +6

    Fantastic my dude. I was wondering if you were going to do anything with morels. I just knew I had to be patient.

    • @blacksheepnomad9000
      @blacksheepnomad9000 3 ปีที่แล้ว

      He's still trying to figure it all out if you haven't kept up

    • @kodyfrost
      @kodyfrost 3 ปีที่แล้ว

      @@blacksheepnomad9000 Iade that comment a year ago dude

    • @blacksheepnomad9000
      @blacksheepnomad9000 3 ปีที่แล้ว +1

      @@kodyfrost and here I am and here you are and he's still trying to grow morel's. time is unimportant. what we do with it is. For instance I'm keeping up with his efforts and your salty I updated you

  • @user-tx5tc4hz9k
    @user-tx5tc4hz9k 7 หลายเดือนก่อน

    It is useful video, . Thank you

  • @glennplatvoet7111
    @glennplatvoet7111 4 หลายเดือนก่อน

    That's nice I'm interested in getting morels going we had drought and there aren't any more it's a shame. Tried getting some established no go 😞

  • @wesstone7571
    @wesstone7571 3 ปีที่แล้ว

    I'll be doing this experiment shortly.

    • @wesstone7571
      @wesstone7571 3 ปีที่แล้ว

      I want to build a few of the media extractors. Having trouble locating parts though

  • @Lulzswag
    @Lulzswag 2 ปีที่แล้ว +3

    I'm amazed by the informative and quality content that you produce. I'm an aspiring hobby mycologist but I have plans of perhaps one day making it official. I've absolutely fallen in love with mycology. I'm starting from practically nothing but I have full intentions of one day having my own mycological business and lab.
    :)
    Mush love

  • @romainarnoux
    @romainarnoux 4 ปีที่แล้ว +2

    Great job! I understand so much about lab thanks to you. Can you describe your tools sterelization process? Thanks again from France

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  4 ปีที่แล้ว +2

      Thanks for watching! We sterilize everything at 15PSI/250F for 20 min (liquid) 60 min (tools) 90 min (substrates) depending on batch sizes and the sterilization pouches or strips are great for confirming sterilization

    • @Lexington-Felix
      @Lexington-Felix ปีที่แล้ว

      @@FreshfromtheFarmFungi HELLO THERE 🙂!
      WAIT SO IS THE HEAT OK FOR MY LC WHILE I'M STERILIZING THE WAY I BELIEVE YOU DID? I DIDNT UNDERSTAND QUITE WELL AND I DON'T WANT TO KILL THE LC THAT I RECENTLY ORDERED? I AM GOING TO INNOCULATE MY GLASS JAR FILLED WITH HONEY AND DEXTROSE (MIXED ALONE) BUT I WAS UNDER THE IMPRESSION THAT YOU SHOULDN'T ALLOW LC TO GET ABOVE 70 DEGREES OR IT DIES. IS THAT CORRECT? EVENTUALLY, I PLAN TO SPORE THE LIQUID CULTURE ON GRAIN AND MANURE SUBSTRATE.
      SORRY FOR THE CAPS I AM NOT SCREAMING JUST DYSLEXIC AND A BIT BLIND🤓😝. I AM NEW TO THIS FIELD AND I AM DILIGENTLY RESEARCHING AS MANY GROWERS AND THEIR PROCESSES AS I POSSIBLY CAN.
      IN YOUR OPINION, WHAT STRAIN DO YOU RECOMMEND IS THE EASIEST AND FASTEST TO FRUIT IN THE MAGICAL MEDICINAL AREA? GROWING TO LEAVE BEHIND THE POISONOUS BIG PHARMA MEDICINE TRAIN TO TREAT MY PTSD, CHRONIC PAIN AND DEPRESSION NATURALLY BY MICRODOSING.
      ANY ADVICE, DIRECTION OR HELP FROM ANYONE IS ALWAYS APPRECIATED. LONG LIVE THE FUNGI WORLD! 💚✌🍄

  • @alencarbatista4976
    @alencarbatista4976 2 ปีที่แล้ว

    Nice Hermano Mushlove 🍄

  • @SomeJustice19k
    @SomeJustice19k 3 ปีที่แล้ว

    Dude, any luck with the morels?! Can't wait to try and clone some next spring!

  • @BOOYAGA
    @BOOYAGA 4 ปีที่แล้ว +1

    Are you active on shroomery or any other forum more suitable for asking questions than YT chat?

  • @NOT0RI0US
    @NOT0RI0US 10 หลายเดือนก่อน

    Hermano me has descrestado con tu herramienta, solo x esto mereces mi suscripción y aca estaré pendiente de tu contenido

  • @richardbinkley8487
    @richardbinkley8487 5 หลายเดือนก่อน

    When you come back after "a few days", was the media being mixed the whole time, or just when you put it on for the videos?

  • @autonomousindividual7780
    @autonomousindividual7780 2 ปีที่แล้ว

    Safe to say.....really excited.

  • @surefiremushroomsmicrogreens
    @surefiremushroomsmicrogreens 3 ปีที่แล้ว +1

    Hey Gary 👋 I gotta get me a media extractor!! Thing is awesome man😎 Does the hose unclip from the jar lid to use on multiple LC Jars or do you have to move the lid and all to another LC container. Cleaning it out in between of course 😉

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  3 ปีที่แล้ว +1

      Hey thanks for watching! They are all one piece so I use a normal lid for storage and have the extractor lids on hand in a sterilization pouch - reach out to Matt at Lions Mane though he would appreciate some feedback and can make custom orders if it’s in his wheelhouse

    • @johnandjacquelinewarren9979
      @johnandjacquelinewarren9979 2 ปีที่แล้ว

      @@FreshfromtheFarmFungi Yeah, I was just watching this and wondering if there is any reason I couldn't make my own DIY version of this lid that would fit on a mason jar. 🤔

  • @jozefjanech1705
    @jozefjanech1705 4 ปีที่แล้ว +1

    Hi Gary. Nice video. I like watching your videos. Please at what intervals do you mix your LC on the mixer. And how long? Sorry for translation. I'm sending you greeting from Slovakia. I wish everyone well.

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  4 ปีที่แล้ว

      thanks for watching! I usually mix once a day for a minute or 2 at the least and sometimes longer to break up larger growth. It can keep mixing as long as it is sterile it won’t harm the mycelium. MUSHLOVE

    • @AspireBlack
      @AspireBlack 3 ปีที่แล้ว +1

      @@FreshfromtheFarmFungi how long could one keep healthy mycelium in a refrigerator, assuming it passes qc?

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  3 ปีที่แล้ว

      @@AspireBlack It really depends on the strains and genetics of the mycelium being stored - I prefer to store genetics on slants long term. But in LC cordyceps will last the shortest before degrading and some species like reishi can last for years.

  • @jjgems6732
    @jjgems6732 2 ปีที่แล้ว

    I would love to hear a follow-up review or comment! Are you still using the media extractor? Also, after the jar is done, is it easy to sanitize and clean the tubing?

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 ปีที่แล้ว +1

      yes I use these still Im making another video with a new ad-on but they are easy to clean I replaced the hose about twice a year because it gets loosened

  • @mattmatthew4648
    @mattmatthew4648 4 ปีที่แล้ว

    Love the videos Gary! Thank you 🙏

  • @mr.b8839
    @mr.b8839 3 ปีที่แล้ว +1

    In the video did you transfer the media extractor from the fresh mix of cordiceps straight to the morchella? (@ 24 min) cross contaminants?

  • @ironhalo696
    @ironhalo696 3 หลายเดือนก่อน

    Great video Gary! Curious how you got all those cracks in the acrylic windows of your flow hood though. I just bought an AirScience Flow like yours and I’d like to avoid that happening!!😮 Keep up the good work man!

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  3 หลายเดือนก่อน +1

      It happened from setting bulk substrate bags against the sides it caused it to heat up and cool down which made the cracks. I replaced them fairly cheap (100$ from the hardware store) but now I only use this hood for culture work and have a horizontal ffu type unit for my bulk substrate inoculations. I love the air science hood it was just a user error on my part haha

    • @ironhalo696
      @ironhalo696 3 หลายเดือนก่อน

      @@FreshfromtheFarmFungi Gotcha, thanks for the heads up!! I'll keep any big heat sources off the panels.

  • @kicknadeadcat
    @kicknadeadcat 3 ปีที่แล้ว +1

    I use my own maple syrup that I make and water. The way I figure It, it comes from a hardwood maple tree. Tress are what mushrooms love. So it contain s the same carbohydrates it’s used to.

    • @seancostello26
      @seancostello26 3 ปีที่แล้ว

      idk how true that is but i love teh logic!

  • @MsJoeporto
    @MsJoeporto ปีที่แล้ว

    Nice and informative videos
    I do have a question fresh from the farm fungi how long can you keep using the same culture before it’s not good

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  ปีที่แล้ว +1

      it depends on each strain - we store them in slant culture tubes to preserve the mother culture for years and years but some will last decades

  • @wigglewigglewiggle7021
    @wigglewigglewiggle7021 3 ปีที่แล้ว

    Back with another question for my Fungi Guru😊
    Question number one when pouring agar into Petri dishes I'm assuming the steam on the lid is from my pouring it in too hot and I should let it cool more unfortunately I already started the process so I just turn the lids upside down for about 10 minutes and then flip them over. What negative effect does the moisture have inside the Petri dishes?
    Second question and I'm sure you probably made a video of it already if so please just tell me the name and I'll go watch it LOL but the question was what is liquid culture made from and why can you store it so much longer than agar Petri dishes

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  3 ปีที่แล้ว +2

      Condensation isn’t a huge deal you can let them dry out in front of a hood - the LC is made from honey and water 👍 It’s a 3d matrix compared to a 2d plate so sustains longer growth

  • @derekb117
    @derekb117 3 ปีที่แล้ว

    dude, thanks so much man. such a great video, no one has made such a in-depth tutorial about liquid culture. why am i not using that extraction technique!!
    idk if you'll read this but can i ask what is your job title?

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  3 ปีที่แล้ว +4

      Hey thanks for following along! I am a mushroom farmer, scientist and entrepreneur - professionally, a Medical Laboratory Scientist but have found a passion for growing food and mushrooms are my gift I embrace MUSHLOVE

  • @RichardNBBarrett
    @RichardNBBarrett 3 ปีที่แล้ว

    This was great. What would be cool is if you attached a third tube to the bottle with a needle on the end and just used the syringe side to draw up and pump out with either a tap or valve to force the liquid outbound. I did see the selection and breeding videos, so awesome. Have you made anything on microscopy for isolation or breeding?

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  3 ปีที่แล้ว +1

      Cool suggestions Ill pass it on to Lion’s Mane equipment supply store 👍 Check out the series on Cordyceps - we did a microscopic ascospore isolation in the first video - I prefer to use dilutions because of the process it’s less time sensitive but both will work

    • @Lalovision3D
      @Lalovision3D ปีที่แล้ว

      If you forced air in to push out liquido culture you would basically be introducing contaminated air into the sterile atmosphere within the jar.
      It defeats the purpose of the jar sterilization process.

    • @RichardNBBarrett
      @RichardNBBarrett ปีที่แล้ว

      @@Lalovision3D obviously you would not use air that was not filtered.

  • @CMAenergy
    @CMAenergy ปีที่แล้ว

    I thought at the 24 min mark when you moved the extractor from one jar to the other jar.
    You did not sterilize the extractor.
    Would that contaminate the culture ?
    Or did I miss something

  • @NOcheeseANDpie
    @NOcheeseANDpie 2 ปีที่แล้ว

    Thank you for the video. How do you make blood agar plates and green plates? What is there except for agar and water? Thank you.

  • @davidsabo405
    @davidsabo405 3 ปีที่แล้ว

    awesome. im excited for you LOL go for it!!!

  • @jeffweld7904
    @jeffweld7904 3 ปีที่แล้ว

    Great demo of the extractor. Tried to order one but they are out of stock "bummer" Also can you show how you make the blood agar for your QC plates?

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  3 ปีที่แล้ว

      Thanks for watching! I think they take pre-orders on their site : ) Also, we purchase our QC plates from hardy diagnostics (that way they are third party tested and certified) but they are made the same way as any other media - MUSHLOVE

  • @kodyfrost
    @kodyfrost 4 ปีที่แล้ว

    What type of agar would you recommend for morel long-term storage? I guess also for other mushrooms as well if the answer would differ

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  4 ปีที่แล้ว +2

      Check out our video on making slants for long term storage - we prefer MEA and PDA because many strains do well with it! You can also lean a toothpick or popsicle stick inside the slant and sterilize to help with even longer storage. MUSHLOVE

  • @madmikey1980
    @madmikey1980 2 ปีที่แล้ว

    I finally had success with inoculation of Morchella around my new house. I found one variable that allowed almost 100% success. All the other spots I tried did not fruit. There’s something that everyone sees every time they hunt Morels but they never take note of it. That’s my secret. 3 years straight with success.
    It’s not really how you make your spore slurry or grow your mycelium but rather, it’s where you put it that matters!

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 ปีที่แล้ว

      cool! when did you spawn? Also, what species?

    • @madmikey1980
      @madmikey1980 2 ปีที่แล้ว

      @@FreshfromtheFarmFungi October. no spawn. Just spores from nothing special except dried morels from spring. Distilled water reconstituted and then 48 hrs with molasses. Then straight into some slippery elm leaf mulch. Then placed in *specific areas*
      No offense to your scientific method but nature doesn’t allow for complete isolation from mirobrobes and does just fine. I’m just a simple man with a simple plan :)

  • @sarahbiate4965
    @sarahbiate4965 3 ปีที่แล้ว

    Please tell me what’s the name of that first gel and the second one? Liquid culture process

  • @Shroomaholics
    @Shroomaholics ปีที่แล้ว

    Hi gary what type of tubing did you use for your LC? I heard some type are permeable.

  • @nataliastaniszewski6006
    @nataliastaniszewski6006 2 ปีที่แล้ว

    How much rice per Lc do you use? Great content btw thank you!

  • @denniswood231
    @denniswood231 2 ปีที่แล้ว

    Hi, so you don't use Peptone any more or don't think you need it...interested to know...thanks!

  • @Dangit-Dave
    @Dangit-Dave 2 ปีที่แล้ว

    Love the importance of QC. What’s the rate of liquid cultures that have failed at the QC point?

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 ปีที่แล้ว +1

      It varies throughout the year because of environmental patterns but usually 3-10% is average for me

  • @tuyetphan2849
    @tuyetphan2849 3 ปีที่แล้ว

    What’s the first gel & second gel please let me know
    Thank you!

  • @FknNefFy
    @FknNefFy 3 ปีที่แล้ว

    Brilliant

  • @macmckulis7440
    @macmckulis7440 ปีที่แล้ว

    Amazing bro good jo.

  • @sebumgold3108
    @sebumgold3108 ปีที่แล้ว

    Hi Gary. I didn't see a cap on that media luer lock connector and I did not see you spray it with alcohol before connecting your sterilized pipette. Any insight into this. Was something missed in editing? Thanks.

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  ปีที่แล้ว

      it was in the flow hood and this was before matt added caps to that contraption- there is a back flow valve so any fluid only went from the check valve forward. Not ideal but it worked

    • @sebumgold3108
      @sebumgold3108 ปีที่แล้ว

      @@FreshfromtheFarmFungi thanks for the reply!

  • @scottholliday9308
    @scottholliday9308 3 ปีที่แล้ว

    Loved your video.
    Do you sell liquid culture? And if so, what would it cost for the moral culture?
    I am just getting into mycology, and the moral is my target.
    I am also getting into different varieties of oyster, and medicinal as well.
    The only thing i don't have is the flow fan and the moral culture, but i am working on that.
    Thank you for your time and your video, very knowledgeable my friend!

  • @theorbologist5228
    @theorbologist5228 3 ปีที่แล้ว

    YOU ARE AWESOME !

  • @jdavid1488
    @jdavid1488 3 ปีที่แล้ว

    is the air exchange of the spawn jars from lids upside down and loosely screwing the bands on?

  • @samkshoemaker
    @samkshoemaker 4 ปีที่แล้ว +1

    Thanks for all your videos. What do you think caused the contams for your Cordyceps LC? 90 minutes at 15 psi was too short? The slant culture? Honey? How do you keep from being paranoid and losing your mind trying to locate the source of the contamination? Would you have had more confidence if you hadn't been making a video. Are you confident the media extractor isn't picking up bacteria with that hanging port?

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  4 ปีที่แล้ว +3

      The contamination probably occurred from poor technique by me during inoculation of the culture into the LC (yeast or bacteria from my skin probably dropping into the vessel - just takes one viable cell to enter the bottle and multiply exponentially). The best way to find out where or when is to leave out blanks or let the LC rest at room temp for a few days before inoculating. It should remain clear if it’s sterile and will plate clean. The original culture was clean because the next round worked fine. There is always human error in mycology however small or rare, which requires a lot of patience and monitoring and trial and error 👍🏻 MUSHLOVE

    • @mysticalsprings1998
      @mysticalsprings1998 2 ปีที่แล้ว

      @@FreshfromtheFarmFungi could be systemic??? Your technique looked good.

  • @reallife2849
    @reallife2849 2 ปีที่แล้ว

    I’ve spread many mushrooms while dropping spores placed them A blender with molasses water mix and store them in jugs shaken every day and spread around my yard . Mushrooms galore . I’ve done this with amanita s as well

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 ปีที่แล้ว +1

      awesome! I always wanted to try this with a backpack sprayer 🍄❤️

    • @reallife2849
      @reallife2849 2 ปีที่แล้ว

      @@FreshfromtheFarmFungi that’s a good idea. I think the reason molasses works well for me is the fact that mushrooms love sulfur and some sugars . Now I’m going to try your method. I’ve never tried honey but it makes sense honey is anti bacterial . I wonder if when I hydrate the honey more, will it lessen the anti bacterial strength. My bees take a long time getting the honey to the exact hydration so it won’t mold . This is very interesting maybe I’ll add a touch of organic sulfur.

  • @yuwonowibowo
    @yuwonowibowo 4 ปีที่แล้ว

    Hello. Nice video. So I wonder if you keep the bottle cap tighly close, not allowing gas trasnfer during lc incubation?

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  4 ปีที่แล้ว

      I screw it tight and make sure there is headspace for fresh air then when mixing in the hood take the cap off to allow for air exchanges. They make these lids with a filter patch as well

    • @yuwonowibowo
      @yuwonowibowo 4 ปีที่แล้ว

      @@FreshfromtheFarmFungi I see. so you mean enough headspace is the key correct? I would like to try growing a smaller culture in 50 mL tube as a seed culture of bigger bottle. I guess it can be done. Thanks

    • @yuwonowibowo
      @yuwonowibowo 4 ปีที่แล้ว

      @@FreshfromtheFarmFungi if the tube screw tight for several days with a lot of head space, (80%), do you think it would be okay to leave it unopened for several days until the cultures fully grown?

  • @emartin8907
    @emartin8907 ปีที่แล้ว

    Hi Gary I am starting to use these media extractor bottle setups and have a question- at 19:00 in you take OFF the lid to re sterilize- why?? Isn't the point of these lids to have to NEVER take the lid off?
    Are you concerned about the liquid in that extraction tube sitting there? I am wondering because when I PC my bottles with these lids on (and not screwed TIGHT just loosely on) that tube will fill up, and I am wondering if I should be concerned with that tube sitting full like that long term?

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  ปีที่แล้ว +1

      Im going to do a followup video on these after a few years use - I took the cap off to clean it thoroughly so I could run another batch through it - they get clogged with mycelium over time and it’s better to stay ahead and keep it clean before it gets plugged. Sitting full shouldn’t effect it as long as it was sterilized properly 👍

    • @emartin8907
      @emartin8907 ปีที่แล้ว

      @@FreshfromtheFarmFungi right on man I look forward to seeing that, and thanks for the quick reply! cheers!

  • @lytespeed.4484
    @lytespeed.4484 2 ปีที่แล้ว

    could you make a video on how to make those lids for the media bottles?

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 ปีที่แล้ว

      matt from lions mane equipment and supply in denver makes them if you reach out he might be able to help 👍🙂

  • @andrewbrandwein312
    @andrewbrandwein312 3 ปีที่แล้ว

    What is the syringe you are using to draw out the liquid? What mls but also tip size (1/4?) What are the complete specs on the glass pipette???????

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  3 ปีที่แล้ว

      I'm not sure it came with the setup - Lion's mane equipment and supply store in Denver has the info on that

  • @kaungkalay128
    @kaungkalay128 3 ปีที่แล้ว

    Can you tell me what you need to make mushroom juice?

  • @djpitr
    @djpitr 3 ปีที่แล้ว

    Any luck on the morel growing ?

  • @GR-sg2lv
    @GR-sg2lv 3 ปีที่แล้ว

    What about an extra long needled syringe that can be reused and re-sterilyzed. Does that exist? I will have to look..

  • @shanesmith89
    @shanesmith89 4 ปีที่แล้ว

    Is that a check valve on the tubing that is dangling off the cap? the second fitting from the end? is that how it stops the liquid from getting contamination? Been trying to hit them up to buy one but no response...

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  4 ปีที่แล้ว +1

      Yes it is a .22 micron filter - they have been working hard to get their store online with all the recent events and he is going to be making some sort of kit in the future - everything is in the "prototype" phase and we are open to taking suggestions! MUSHLOVE

    • @shanesmith89
      @shanesmith89 4 ปีที่แล้ว

      @@FreshfromtheFarmFungi That's the air intake side right? I mean the side where you withdraw the liquid. That has a filter too? or how is it keeping air from backflowing inside?

    • @jeromecalderone4526
      @jeromecalderone4526 4 ปีที่แล้ว

      @@shanesmith89 thats a one way check valve

  • @johnnyfish6051
    @johnnyfish6051 ปีที่แล้ว +1

    Love mushrooms!

  • @seattlednb
    @seattlednb 2 ปีที่แล้ว

    How come the grain jars you don’t have the self injecting port or a gas exchange filter disk?

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 ปีที่แล้ว

      the lids flipped like this and the threading is enough space for fresh air exchange

  • @Beinhartwie1chopper
    @Beinhartwie1chopper 2 ปีที่แล้ว

    You meant grain spawn......also a Injektion port on the jars would be good

  • @funghi-farm
    @funghi-farm 3 ปีที่แล้ว

    is a really good video ! Can ibuy them in Europa?

  • @rhysezfpv
    @rhysezfpv 2 ปีที่แล้ว

    what do you call those hose that you connect to the top of your reagent bottle ? Im looking for those to use with mine thanks :)

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 ปีที่แล้ว

      Flex tubing, pharmed tubing, silocon tubing it is common in most micro labs

  • @sayuas4293
    @sayuas4293 ปีที่แล้ว

    Is there a reason that you need to use these media bottles for LC instead of just using ball jars?

  • @davidfelts5150
    @davidfelts5150 4 หลายเดือนก่อน

    Is the blood agar required or could you do the QC with potato flake agar?

  • @nickagamaite7397
    @nickagamaite7397 2 ปีที่แล้ว

    Where did you buy the extractor? Mushlove

  • @bullishdan2436
    @bullishdan2436 3 ปีที่แล้ว

    I’m wondering if I am the only one concerned that you just pulled the tube from the Cordyceps jar and placed it directly into the morel jar? Wouldn’t their be Cordyceps mycelium in the tube still.. and also, isn’t that running the risk of transferring a possible contaminant from one jar to the next? How much are these extractor tops? Seems like it would be better to have one for each jar.. or am I over-thinking it? Regardless.. great video. Thanks for sharing.

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  3 ปีที่แล้ว +1

      It’s a concern yes but the lid was sterilized between - cross contamination can be between different mushrooms though good observation - MUSHLOVE

  • @levittperez
    @levittperez 6 หลายเดือนก่อน

    What size pressure cooker do you use?

  • @LiquidCultureGod
    @LiquidCultureGod 9 หลายเดือนก่อน

    Referencing to when he says use just honey : Use whatever broth for the LC you want and whatever works best for you, you're not gonna be able to see contamination anyways and you should always be running agar before you noc anything

  • @labpartners3490
    @labpartners3490 ปีที่แล้ว

    Don't have to speak directly into mic, 20 something in, almost blew my speakers lol. Thanks so much for the help, though. Can't wait to get some started myself.

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  ปีที่แล้ว

      yes the audio on these older videos was bad Im going to put captions in this winter but the newer videos are better ha

  • @tomchrissuicagoins1900
    @tomchrissuicagoins1900 3 ปีที่แล้ว

    Can you please specify the types of QC agar you are using their names I cannot transcribe and find of hardy s website.

  • @ScoopingCelebrities
    @ScoopingCelebrities 3 ปีที่แล้ว

    how can i alcohol extract mycelium, lions mane?

  • @justinwheeler1979
    @justinwheeler1979 ปีที่แล้ว

    When will it be back in stock

  • @rwood1295
    @rwood1295 3 ปีที่แล้ว

    If you were to grow out the contaminated mycelium would the mushrooms be poisonous??

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  3 ปีที่แล้ว

      My talk on contamination - treat mushrooms like meat and cook thoroughly m.th-cam.com/video/ssxdhydPYos/w-d-xo.html

  • @brianagiraldo-frendo7442
    @brianagiraldo-frendo7442 3 ปีที่แล้ว

    So you don't need a gas exchange filter to colonize LC?

  • @potumnn
    @potumnn 2 ปีที่แล้ว

    17:05 just loving it

  • @travisslater5256
    @travisslater5256 4 ปีที่แล้ว

    Where can i buy a media extractor
    Also check manuka honey it has a peroxide effect

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  4 ปีที่แล้ว +2

      Message the owners of Lion’s Mane Equipment and Supply in Denver lionsmanedenver.com

  • @cjmac3636
    @cjmac3636 4 ปีที่แล้ว

    what is the green and red QC plate you got from diagnostics ?

    • @cjmac3636
      @cjmac3636 4 ปีที่แล้ว

      sorry answering own question - please see this video for full description
      th-cam.com/video/iHYBu3rXHes/w-d-xo.html

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  4 ปีที่แล้ว +1

      Yes - the green is HE and the red is SBA - both are commonly used screening QC plates

  • @_Adam93
    @_Adam93 3 ปีที่แล้ว

    Where can I find transfer caps with filters like that!

  • @mashay4737
    @mashay4737 3 ปีที่แล้ว

    How Mach time on proses airator liquid culture

  • @quailsnest8354
    @quailsnest8354 4 ปีที่แล้ว

    how do you know what temperture you are at with the pressure cooker,, says, 250F..

  • @ThePinkBinks
    @ThePinkBinks 2 ปีที่แล้ว +2

    I’ll modify an autoclavable handsoap bottle. Thanks. 😁

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 ปีที่แล้ว +1

      cool idea! innovation at its finest 🍄❤️

    • @ThePinkBinks
      @ThePinkBinks 2 ปีที่แล้ว

      Fresh from the Farm Fungi LLC Ditto. 😁

  • @user-rz6ei7dj8r
    @user-rz6ei7dj8r 3 ปีที่แล้ว

    May I buy MOREL liquid spawn? Can you send to THAILAND? If posible please give me the price+shipping and payment method such as Pay pal or Western union. Thanks

  • @shuckahoseerazzle8486
    @shuckahoseerazzle8486 ปีที่แล้ว

    4-5 days of cooling to room temp?

  • @tuyetphan2849
    @tuyetphan2849 3 ปีที่แล้ว

    What you’re put in there look like the pill?
    Thank you!

    • @jwmcguire3
      @jwmcguire3 3 ปีที่แล้ว

      Magnetic stirrer