If you have an old coffee can or something or the sort/size, you can set it on the burner on your stove for a bit to heat up and then melt the hand/arm holes into the SAB. Wont have to worry about cracking the tub either while cutting/drilling!
And I seen how they put the gorilla tape on each side of the hole since it's a big coffee size hole and cut an x in the tape sticky to sticky over the hole like a skirt, maybe not necessary but seems like a good design
@@MycoDogsMan, I can't even tell you how much. I would appreciate it if you would at least make a quick video about how to put that sterilizer together. I ordered the parts. I just don't really have the technical knowledge I'm not finding anything online
I'd love to see that induction sterilizer video you mentioned. Got the parts loaded into my Amazon basket, just need the guidance on how it all goes together. Thanks for your work!
I don’t use alcohol inside the SAB anymore, because I have a strong UV-C light inside. I turn it on for 15 minutes to sterilize the inside of the SAB before I start working, and turn it off when I have to sit next to it, or else I would go blind, lol. Then I use alcohol on everything that enters the SAB before I put it through the arm holes. Never had anything contaminated since I started doing it this way. I even let Petri dishes harden/cool without the lids on inside the box, so I avoid condensation, and just have the UV-C light turned on the entire time the lids are off. 😊
I do as well, but you have to are sure nothing living is in the room when using the light. And I still use alcohol as a backup. I’ve never experienced contamination.
It's pretty easy to have low contamination rates with a SAB if you're already in a fairly clean environment, but only a laminar flow setup will still give you low contamination rates in less than ideal conditions as well. I rent an old house in Florida with windows that are overlapping glass strips. I can clean the house from top to bottom, run an air purifier 24/7, and have the sterile technique of an open heart surgeon and a SAB still gives me 65%+ contamination because no matter what, there's a constant supply of contamination entering the house. Nearly zero contamination with a FFU.
Quite a few explosions have occurred when flame sterilising needles inside still air boxes. If the alcohol vapour is in the right proportions with the air (upper/lower explosion limit), then it will ignite/explode. So, be careful and only use the minimum amount of alcohol when wiping down surfaces, and no open flames.
Yo im trying to get to know as mush as possible before beginning to even try to grow my own mushies and damn youre videos are amazing keep it up!!! i learned so much already
Appreciate the video! Compact and full of information! Never the less, because we are in the topic sterile workflow, does somebody have any tips on putting the grainspawn into bulk in a monotub in the most steril way possible. Its my first time soon and i am a bit paranoid about Contamination. Obviously i dont have a flow tube neither a Still Air Box big enough to but my Monotub in.
Hey, i appericiate your Video, thanks for all the practical information. I got one question. You said you wouldnt recommend growing mushrooms for a profit. Can you explain why ? You can start with a relatively low budget and if you work clean you get constant results. Im thinking about delivering my mushrooms fresh to the local gastronomy of my village. Why cant you recommend ? Or cant you just recommend it for the us ?
I personally haven't had any issues using it directly after spraying (i do it basically every time i use it) so its definitely a lot safer than an open flame but there could be some edge cases that could ignite the fumes so still be careful
I always used a ghetto-ass but very effective method known on the interwebs as a 'shmuvbox'. You tape a transparent trash bag to the outlet of a small room air filter with a UHPA cartridge, then cut armholes in the inflated bag. Bags are inherently sterile inside because of the way they're manufactured, but you can wipe it down after it's inflated for an extra bump in security. You can do anything you can do in front of a flowhood in it, and it costs very little.
I have seen this done before, I'm sure you can have success with it. There is an issue with a setup like this is the airflow inside the bag is very turbulent and aggressive, meaning if your arms or equipment have any contam on them it will get blown all over the place. A still airbox on the other hand u can rub ur hands around in dirt and work without washing them without contam because there is no turbulence
@@MycoDogs yeah, you're absolutely right. I always used veterinary gloves that went way up past my elbows and only performed one task at a time, wiping down nearly constantly. I live somewhere pretty humid, with a VERY high spore load, and have always had indoor pets and lived in old drafty farmhouses, the glovebox (still air box) was never effective for me. I understand that it's the next best from a flowhood for most folks. I eventually built a flowhood (I'm a commercial electrician who has built a couple of commercial-scale clean rooms for surgical implant manufacturing facilities, so I sourced all the materials for free, I'd never recoup the cost to buy one) and never looked back, but the good ol' shmuv served me well for a long time.
Newb here. Why do people sterilize everything with alcohol but always use heat for the blade? Can you just use alcohol? I'm sure there's a reason. I'm just curious.
alcohol doesn't sterilize, it sanitizes. to be considered sterile it needs to be devoid of ALL life, isopropyl takes care of like 99.9% of it but that 0.01% means a ton when ur talking about sterility. Nothing survives heat so if u heat the blade you can be sure theres no bacteria endospores or anything hiding on it
Very concise and informative content to a newbie trying to learn as much about this as I can. Between you, 90 second mycology, Bass drop keys and willy mico, I'll have a first full flush in no time! Much love to all you guys inspiring us just getting started!
Bro, your induction coil sterilization set up is absolutely awesome! I just got my supplies in today to make one! Thank you for sharing and linking the supplies!
I immediately ordered all the supplies. I have no idea how to put this thing together but I'm going to do my best to figure it out. I wish you would have made that video @mycodogs
What are the dimensions of the SAB? The tubs that you listed are definitely not big enough. I just don't want to waste any money getting a box that's too small and you have no link for one.
Super cool!!! Thank you for sharing this awesome information!!!! Would love to see a video on the induction heater.. Really beautiful rhizomorphic mycelium on that plate!!!!
Flipping the lid over...so simple and easy, sure wish I'd seen this video when I started. Drilling holes in lids is a pain. Where do you get rye grain berries and are they worth the expense? I've had pretty good success with popcorn.
Ah, we have us a smart guy, huh? Let’s review the three mistakes in your single seven word question. *everyone *pronounce *end with question mark, not a period Don’t worry about his pronunciation. Worry about the fact you type like you’re slam drunk.
Thank you for infos. Do you say that the liquid culture jar doesnt need a filter to breath? This means that i can make a needle biopsy, suck some sterilized liquid and air into it and mycelium can grow inside the syringe?
Man frfr, I have been a part of the community and a hobbyist mycologist for some years. But this is the thing is I have a Flow hood, also a FFU, and my old friend my Still Air Box. My SAB has been old faithful. Heck I have the FH, and my FFU. And still this I use my SAB for certain things, small jobs. Lol PLUS I HAVE HER ROCK SLAPS EVERYWHERE. I GOT MY FH COVERED IN THEM TO!!!! Man they look great. But my FFU is clean no slaps on her. But I agree 100% with everything you covered. I do certain things like you, but some other I do different. Plus you showed me some things to try out myself. Keep up the great work.
Too cut the holes use a razor blade and vice grips and a torch.. clamp the blade at and angle away from you hest the blade till red hot and cut like butter
Ive been having very good results working in open air. Just shutting the bathroom door with a towel at bottom and turning on a hot shower till steam covers everything and spraying the air around and over me with alcohol and quickly bang it out. Im bout to make a diy flowhood i think just to reduce the occasional failure. The most important parts imo is making sure your grains are fully sterelized, fully colonized, and your substrate isnt to wet.👍
that is another way to achieve a clean work area, i thought about mentioning it in this video but it was already so long I'll probably mention it in another video down the road
now theres a diff vid from someone else talkung bout how to make a floodhood and why still air boxes are bad lmao youtubes algorithmsr wierd. i think ill stick to a still air box lol
not sure but ii hated his vibe, it waslike a cocky vibe maybe only i felt but like... saying someothing along the lines of "still ar boxes dont work" or something, im not quoting properly but .. gah i just hated him lol. im happy i found ur page! ur making it easy for a dingbat like me to understand and its giving me hopeto start my myco journey!@@MycoDogs
Off topic of the video, but what type of lighting and how much for the all in one grow bags? Trying to remain reasonable on price as I am new to this. Thanks for any help and really enjoying the channel. Scratch that. Just went to the discord channel and that was the most recent post.🤣
Curious - I'm curious, you said at around 1:53 min that you don't encourage others to grow mushrooms for profit. Why did you say that? That was an odd comment to throw in there...
Why would you do this? It has no benefits. The fruits grow in open air and nearly all contamination is from already contaminated spawn, not from airborne contaminants after spawning to bulk. You can stick a tub on top of the fridge and it'll be just fine.
Very interested in how you built/installed that induction coil. Would love to see a video on that. Great work as always!
I agree
th-cam.com/video/AOMdFAOTbmA/w-d-xo.html
YESYESYES
Yeah please make this video man I need this
Oooooj yeez please man!
If you have an old coffee can or something or the sort/size, you can set it on the burner on your stove for a bit to heat up and then melt the hand/arm holes into the SAB. Wont have to worry about cracking the tub either while cutting/drilling!
I did that with dog food lol 22 years ago 😅
This comment is underated
And I seen how they put the gorilla tape on each side of the hole since it's a big coffee size hole and cut an x in the tape sticky to sticky over the hole like a skirt, maybe not necessary but seems like a good design
Very nice video. Good to see someone using an SAB correctly and smoothly :)
Thanks Ed I'm glad u enjoyed it
@@MycoDogsMan, I can't even tell you how much. I would appreciate it if you would at least make a quick video about how to put that sterilizer together. I ordered the parts. I just don't really have the technical knowledge I'm not finding anything online
I'd love to see that induction sterilizer video you mentioned. Got the parts loaded into my Amazon basket, just need the guidance on how it all goes together. Thanks for your work!
that damn coil worked like MAGIC
I would love to see a video on how to build the induction sterilizer. Great video thank you 😊
I don’t use alcohol inside the SAB anymore, because I have a strong UV-C light inside. I turn it on for 15 minutes to sterilize the inside of the SAB before I start working, and turn it off when I have to sit next to it, or else I would go blind, lol.
Then I use alcohol on everything that enters the SAB before I put it through the arm holes. Never had anything contaminated since I started doing it this way.
I even let Petri dishes harden/cool without the lids on inside the box, so I avoid condensation, and just have the UV-C light turned on the entire time the lids are off. 😊
I do as well, but you have to are sure nothing living is in the room when using the light. And I still use alcohol as a backup. I’ve never experienced contamination.
Can you point me to the lamp youre using
It's pretty easy to have low contamination rates with a SAB if you're already in a fairly clean environment, but only a laminar flow setup will still give you low contamination rates in less than ideal conditions as well. I rent an old house in Florida with windows that are overlapping glass strips. I can clean the house from top to bottom, run an air purifier 24/7, and have the sterile technique of an open heart surgeon and a SAB still gives me 65%+ contamination because no matter what, there's a constant supply of contamination entering the house. Nearly zero contamination with a FFU.
Quite a few explosions have occurred when flame sterilising needles inside still air boxes. If the alcohol vapour is in the right proportions with the air (upper/lower explosion limit), then it will ignite/explode. So, be careful and only use the minimum amount of alcohol when wiping down surfaces, and no open flames.
Thank you my good sir for sharing your hard earned experience with us freely! Boundless Love & Light to you❤
Used a industrial led work light and it is connected to a remote switch so I can "no touch" operate it.
Yo dude! Just gotta say the advise and information you gave me when I first started has been so helpful!. I appreciate you! Borf!
I really hope to see that induction sterilizer tutorial someday! It's pretty awesome!
Great video
Damn that induction sterilizer looks awesome
Yo im trying to get to know as mush as possible before beginning to even try to grow my own mushies and damn youre videos are amazing keep it up!!! i learned so much already
I love your vids bro . Remember, “ignorance is bliss , knowledge is power “ - nava
I have a 1000watt induction heater it only cost about 48$ years ago. So i know there are cheap ones.
I'm gonna make a check list using what you said it's so perfect thanks
Appreciate the video! Compact and full of information! Never the less, because we are in the topic sterile workflow, does somebody have any tips on putting the grainspawn into bulk in a monotub in the most steril way possible. Its my first time soon and i am a bit paranoid about Contamination. Obviously i dont have a flow tube neither a Still Air Box big enough to but my Monotub in.
Thanks for sharing .. I like your style 😎
Biggest problem for me is reading to be graduations on the syringe when using a still air box
To put my hand holes in, I heated the top of a coffee can on the stove and melted the holes through. ..Nice and smooth with no cracks.
Awesome video thanks my bruda!
Thanks!!
Really excellent info done well b
12:16 the gloves looking all unloved 😂
Dude. I love you ❤️
Hey, i appericiate your Video, thanks for all the practical information. I got one question. You said you wouldnt recommend growing mushrooms for a profit. Can you explain why ? You can start with a relatively low budget and if you work clean you get constant results. Im thinking about delivering my mushrooms fresh to the local gastronomy of my village. Why cant you recommend ? Or cant you just recommend it for the us ?
i dont recommend it because in the majority of locations they will lock you in a cage if they find out
What I wanna know is the song from 13:50 😂
on.soundcloud.com/KC145
Can a grow tent be used for doing lab work if the exhaust fan is blowing clean air in through the HEPA filter?
It might be better to seal the tent, run the filter inside it for like 30 min then shut it off and wait til the clean air is still in there
I need a video of how u made the sab.. the santizer element and all.
I'll make it at some point its in my plans already
Get a tote and cut two holes large enough for your arms. Pretty simple.
Is it safe to use the induction sterilizer after you spray Isopropyl?
I personally haven't had any issues using it directly after spraying (i do it basically every time i use it) so its definitely a lot safer than an open flame but there could be some edge cases that could ignite the fumes so still be careful
I always used a ghetto-ass but very effective method known on the interwebs as a 'shmuvbox'. You tape a transparent trash bag to the outlet of a small room air filter with a UHPA cartridge, then cut armholes in the inflated bag. Bags are inherently sterile inside because of the way they're manufactured, but you can wipe it down after it's inflated for an extra bump in security. You can do anything you can do in front of a flowhood in it, and it costs very little.
I have seen this done before, I'm sure you can have success with it. There is an issue with a setup like this is the airflow inside the bag is very turbulent and aggressive, meaning if your arms or equipment have any contam on them it will get blown all over the place. A still airbox on the other hand u can rub ur hands around in dirt and work without washing them without contam because there is no turbulence
@@MycoDogs yeah, you're absolutely right. I always used veterinary gloves that went way up past my elbows and only performed one task at a time, wiping down nearly constantly. I live somewhere pretty humid, with a VERY high spore load, and have always had indoor pets and lived in old drafty farmhouses, the glovebox (still air box) was never effective for me. I understand that it's the next best from a flowhood for most folks. I eventually built a flowhood (I'm a commercial electrician who has built a couple of commercial-scale clean rooms for surgical implant manufacturing facilities, so I sourced all the materials for free, I'd never recoup the cost to buy one) and never looked back, but the good ol' shmuv served me well for a long time.
I rather have still air than cheap air
Newb here. Why do people sterilize everything with alcohol but always use heat for the blade? Can you just use alcohol? I'm sure there's a reason. I'm just curious.
alcohol doesn't sterilize, it sanitizes. to be considered sterile it needs to be devoid of ALL life, isopropyl takes care of like 99.9% of it but that 0.01% means a ton when ur talking about sterility. Nothing survives heat so if u heat the blade you can be sure theres no bacteria endospores or anything hiding on it
This video needs more views do u have a discord
Buy a flow hood. You will thank yourself.
My wallet would not thank me
Flow hoods a thousand dollers? Not if your smart..100$ Way better than a SAB.
just the filter for a real flow hood is $400 on average
@@MycoDogs mines "real" and it was 80$, just a tru hepa filter and a proper fan and box..
stop with the facts
I ve seen cleaner hands on a zombie
like it or not this is what peak male performance looks like
Very concise and informative content to a newbie trying to learn as much about this as I can. Between you, 90 second mycology, Bass drop keys and willy mico, I'll have a first full flush in no time! Much love to all you guys inspiring us just getting started!
PGT is another great TH-camr
Bro, your induction coil sterilization set up is absolutely awesome! I just got my supplies in today to make one! Thank you for sharing and linking the supplies!
I immediately ordered all the supplies. I have no idea how to put this thing together but I'm going to do my best to figure it out. I wish you would have made that video @mycodogs
What are the dimensions of the SAB?
The tubs that you listed are definitely not big enough.
I just don't want to waste any money getting a box that's too small and you have no link for one.
Definitely interested in how to build that induction coil
Those induction coils are cheap if you know how to make one. Use a zvs and a coil with center tap.
Super cool!!! Thank you for sharing this awesome information!!!! Would love to see a video on the induction heater.. Really beautiful rhizomorphic mycelium on that plate!!!!
I've had massive success with still air boxes. Love your vids!! ❤️
you should get more views, your content is amazing!❤
fr so much awesome info
Id love to see the setup on that induction sterilizer the only diy videos I can find don’t use a pedal and I want to incorporate it for sure.
Totally interested in DIY induction coil sterilizer video. Thanking you in advance :)
Please do a tutorial on the sterilizer:)
Excellent info! Just the nuts and bolts and presented in a digestible manner. Thanks for sharing your expertise/experience. Subscribed!
Even when making money doing this, a glove box can be enough of a steh·ruhl solution. But I built a flow hood because the box is annoying af.
Would definitely love to see a assemble video on that induction coil fr
Flipping the lid over...so simple and easy, sure wish I'd seen this video when I started. Drilling holes in lids is a pain.
Where do you get rye grain berries and are they worth the expense? I've had pretty good success with popcorn.
Why does wveryone peonounce still like steel.
I can't comment for wveryone, but sometimes it happens
You're high bro
You mean pronounce
Ah, we have us a smart guy, huh? Let’s review the three mistakes in your single seven word question.
*everyone
*pronounce
*end with question mark, not a period
Don’t worry about his pronunciation. Worry about the fact you type like you’re slam drunk.
6:40 sounds like you discovered a flash sterilization method 😂
Thank you for infos. Do you say that the liquid culture jar doesnt need a filter to breath? This means that i can make a needle biopsy, suck some sterilized liquid and air into it and mycelium can grow inside the syringe?
FYI, Those Sterlite Containers have 2 Holes at the lid level near the handles and make great connections to air tubing and air stone in water vases.
I can't stand the cheesy stock video clips that seemingly all TH-camrs use. They are terrible.
Loads more actually good advice you should have more views than my rookie mycologist
Wouldnt go back to a SAB after using my flowhood for the past year! Way less tams and way more convenient!
Man frfr, I have been a part of the community and a hobbyist mycologist for some years. But this is the thing is I have a Flow hood, also a FFU, and my old friend my Still Air Box. My SAB has been old faithful. Heck I have the FH, and my FFU. And still this I use my SAB for certain things, small jobs. Lol PLUS I HAVE HER ROCK SLAPS EVERYWHERE. I GOT MY FH COVERED IN THEM TO!!!! Man they look great. But my FFU is clean no slaps on her. But I agree 100% with everything you covered. I do certain things like you, but some other I do different. Plus you showed me some things to try out myself. Keep up the great work.
7:24 It is able to start the alcohol to burning isnt't it? What is visibly glowing hot that is well over 500C degree.
Too cut the holes use a razor blade and vice grips and a torch.. clamp the blade at and angle away from you hest the blade till red hot and cut like butter
I'm currently building the GordoTek "flowhood" My place is pretty humid and dusty. Great video btw
What if you fart in one? 😮😂
That will probably grow LBMs
Excellent information. Many Thanks
INDUCTION STERILIZER VIDEO NOW PLEASE SIR!!!!
vielen dank.
Noobs HATE him for this one simple trick
This is very well thought out and described. Thanks!
This is a fantastic video, your technique is masterful
A 6 inch hole saw ran in reverse should cut a quick hole
Built my laminar blow box for 65 bucks.
Wish i had watched this befire ordering everytbing to build a lam flow hood. Guess ill use both methods. My first attemot at reishis.
Flow hoods are definitely a good quality of life upgrade especially if ur doing a lot of work
Man i just love how you explain stuff
I can watch this all day
Are you sure clean rooms aren't sterile? I'm almost certain the clean rooms at a chip fab are completely sterile.
i know they are very very very clean but I'm sure there are 1 or two stray cells floating around the room, sterile means zero life at all anywhere
Ive been having very good results working in open air. Just shutting the bathroom door with a towel at bottom and turning on a hot shower till steam covers everything and spraying the air around and over me with alcohol and quickly bang it out. Im bout to make a diy flowhood i think just to reduce the occasional failure. The most important parts imo is making sure your grains are fully sterelized, fully colonized, and your substrate isnt to wet.👍
yea ive done transfers just on my desk and they work if ur careful
6:29 And the air sterilized suddenly. :D
Too late; bought one for under 500, have both
good stuff!
Right on! 🤟🏻 I appreciate content that I learn from
You ever melted bag near that induction
If reuse-able glass petri dishes are sterilised in a pressure canner, how to get them into a bag for later use without contamination?
I wrap them in tin foil and keep it wrapped til I use them
@@MycoDogs Brilliant, thankyou.
A guy with nails like that talking about a sterlie environment... 😂
even more proof technique matters more than anything else
video tutorial for the induction sterilizer, I love it 🙏🙌
You don't need a sab. Work in Front of a bunsen burner
that is another way to achieve a clean work area, i thought about mentioning it in this video but it was already so long I'll probably mention it in another video down the road
now theres a diff vid from someone else talkung bout how to make a floodhood and why still air boxes are bad lmao youtubes algorithmsr wierd. i think ill stick to a still air box lol
Here's my question, does that guy have a financial incentive for you to buy a flow hood?
not sure but ii hated his vibe, it waslike a cocky vibe maybe only i felt but like... saying someothing along the lines of "still ar boxes dont work" or something, im not quoting properly but .. gah i just hated him lol. im happy i found ur page! ur making it easy for a dingbat like me to understand and its giving me hopeto start my myco journey!@@MycoDogs
I’m very interested in how to build the induction coil into the SAB, appreciate if you can make a video about it! Mush Love ❤️
ha nice furry mask
Thumbnail says "DUBUNKED" instead of "DEBUNKED". Just mentioning it if you feel like fixing it haha. Grest video, thanks a lot 👍
i keep my plates upside down as well while i transfer.. got the idea from you
Off topic of the video, but what type of lighting and how much for the all in one grow bags? Trying to remain reasonable on price as I am new to this. Thanks for any help and really enjoying the channel.
Scratch that. Just went to the discord channel and that was the most recent post.🤣
Curious - I'm curious, you said at around 1:53 min that you don't encourage others to grow mushrooms for profit. Why did you say that? That was an odd comment to throw in there...
Thanks bro
Looking to get a SAB for tissue culture. I found this video super helpful. I'm very interested in the induction sterilizer. It's absolutely brilliant.
Working in front of a ffu is such a QOL improvement. I hated working in a SAB
Look for the video on the induction sterilizer. Id like to know how to make it. Please make a video or share the link where i can find it. Mush love
1:50 "70 bucks" said Madden xD hahaha i reconize that microscope!
Nice to see you! Peace!
Can you put your monotub in a still air box and fruit in it
Why would you do this? It has no benefits. The fruits grow in open air and nearly all contamination is from already contaminated spawn, not from airborne contaminants after spawning to bulk. You can stick a tub on top of the fridge and it'll be just fine.
@ObakuZenCenter do I need my monotub in a still air box. Do you know what your doing? If so i have questions