Very concise and informative content to a newbie trying to learn as much about this as I can. Between you, 90 second mycology, Bass drop keys and willy mico, I'll have a first full flush in no time! Much love to all you guys inspiring us just getting started!
If you have an old coffee can or something or the sort/size, you can set it on the burner on your stove for a bit to heat up and then melt the hand/arm holes into the SAB. Wont have to worry about cracking the tub either while cutting/drilling!
And I seen how they put the gorilla tape on each side of the hole since it's a big coffee size hole and cut an x in the tape sticky to sticky over the hole like a skirt, maybe not necessary but seems like a good design
@@MycoDogsMan, I can't even tell you how much. I would appreciate it if you would at least make a quick video about how to put that sterilizer together. I ordered the parts. I just don't really have the technical knowledge I'm not finding anything online
I'd love to see that induction sterilizer video you mentioned. Got the parts loaded into my Amazon basket, just need the guidance on how it all goes together. Thanks for your work!
Bro, your induction coil sterilization set up is absolutely awesome! I just got my supplies in today to make one! Thank you for sharing and linking the supplies!
I immediately ordered all the supplies. I have no idea how to put this thing together but I'm going to do my best to figure it out. I wish you would have made that video @mycodogs
I don’t use alcohol inside the SAB anymore, because I have a strong UV-C light inside. I turn it on for 15 minutes to sterilize the inside of the SAB before I start working, and turn it off when I have to sit next to it, or else I would go blind, lol. Then I use alcohol on everything that enters the SAB before I put it through the arm holes. Never had anything contaminated since I started doing it this way. I even let Petri dishes harden/cool without the lids on inside the box, so I avoid condensation, and just have the UV-C light turned on the entire time the lids are off. 😊
I do as well, but you have to are sure nothing living is in the room when using the light. And I still use alcohol as a backup. I’ve never experienced contamination.
Super cool!!! Thank you for sharing this awesome information!!!! Would love to see a video on the induction heater.. Really beautiful rhizomorphic mycelium on that plate!!!!
It's pretty easy to have low contamination rates with a SAB if you're already in a fairly clean environment, but only a laminar flow setup will still give you low contamination rates in less than ideal conditions as well. I rent an old house in Florida with windows that are overlapping glass strips. I can clean the house from top to bottom, run an air purifier 24/7, and have the sterile technique of an open heart surgeon and a SAB still gives me 65%+ contamination because no matter what, there's a constant supply of contamination entering the house. Nearly zero contamination with a FFU.
Man frfr, I have been a part of the community and a hobbyist mycologist for some years. But this is the thing is I have a Flow hood, also a FFU, and my old friend my Still Air Box. My SAB has been old faithful. Heck I have the FH, and my FFU. And still this I use my SAB for certain things, small jobs. Lol PLUS I HAVE HER ROCK SLAPS EVERYWHERE. I GOT MY FH COVERED IN THEM TO!!!! Man they look great. But my FFU is clean no slaps on her. But I agree 100% with everything you covered. I do certain things like you, but some other I do different. Plus you showed me some things to try out myself. Keep up the great work.
Quite a few explosions have occurred when flame sterilising needles inside still air boxes. If the alcohol vapour is in the right proportions with the air (upper/lower explosion limit), then it will ignite/explode. So, be careful and only use the minimum amount of alcohol when wiping down surfaces, and no open flames.
Ive been having very good results working in open air. Just shutting the bathroom door with a towel at bottom and turning on a hot shower till steam covers everything and spraying the air around and over me with alcohol and quickly bang it out. Im bout to make a diy flowhood i think just to reduce the occasional failure. The most important parts imo is making sure your grains are fully sterelized, fully colonized, and your substrate isnt to wet.👍
Yo im trying to get to know as mush as possible before beginning to even try to grow my own mushies and damn youre videos are amazing keep it up!!! i learned so much already
Flipping the lid over...so simple and easy, sure wish I'd seen this video when I started. Drilling holes in lids is a pain. Where do you get rye grain berries and are they worth the expense? I've had pretty good success with popcorn.
What are the dimensions of the SAB? The tubs that you listed are definitely not big enough. I just don't want to waste any money getting a box that's too small and you have no link for one.
Too cut the holes use a razor blade and vice grips and a torch.. clamp the blade at and angle away from you hest the blade till red hot and cut like butter
Having the flow hood just removes most of those prep step, it's a huge comfort increase. Of course it comes at a cost, I'd say mine cost around 300€ to build, most of that coming from the fan and filter
I'm not saying anything bad about flow hoods, they make workflow faster and easier especially at scale, just most people don't need to spend that much money especially when just getting into the hobby and if they are experiencing contams in an SAB its not because of equipment its more due to their technique and preparations
Appreciate the video! Compact and full of information! Never the less, because we are in the topic sterile workflow, does somebody have any tips on putting the grainspawn into bulk in a monotub in the most steril way possible. Its my first time soon and i am a bit paranoid about Contamination. Obviously i dont have a flow tube neither a Still Air Box big enough to but my Monotub in.
Thx Bloke, much appreciated. Best thing i did , was make a SAB, before i even colonized my 1st rice bag :)..Makes good sense..makes good practice. Also, tyvm for the diff tech tips, agar plates etc. Some great ideas/best practice strategies. Cya down in the Lab..BLACK TAR GANG! 😎💨💨🖤💯🤘
Off topic of the video, but what type of lighting and how much for the all in one grow bags? Trying to remain reasonable on price as I am new to this. Thanks for any help and really enjoying the channel. Scratch that. Just went to the discord channel and that was the most recent post.🤣
Thank you for infos. Do you say that the liquid culture jar doesnt need a filter to breath? This means that i can make a needle biopsy, suck some sterilized liquid and air into it and mycelium can grow inside the syringe?
Curious - I'm curious, you said at around 1:53 min that you don't encourage others to grow mushrooms for profit. Why did you say that? That was an odd comment to throw in there...
I always used a ghetto-ass but very effective method known on the interwebs as a 'shmuvbox'. You tape a transparent trash bag to the outlet of a small room air filter with a UHPA cartridge, then cut armholes in the inflated bag. Bags are inherently sterile inside because of the way they're manufactured, but you can wipe it down after it's inflated for an extra bump in security. You can do anything you can do in front of a flowhood in it, and it costs very little.
I have seen this done before, I'm sure you can have success with it. There is an issue with a setup like this is the airflow inside the bag is very turbulent and aggressive, meaning if your arms or equipment have any contam on them it will get blown all over the place. A still airbox on the other hand u can rub ur hands around in dirt and work without washing them without contam because there is no turbulence
@@MycoDogs yeah, you're absolutely right. I always used veterinary gloves that went way up past my elbows and only performed one task at a time, wiping down nearly constantly. I live somewhere pretty humid, with a VERY high spore load, and have always had indoor pets and lived in old drafty farmhouses, the glovebox (still air box) was never effective for me. I understand that it's the next best from a flowhood for most folks. I eventually built a flowhood (I'm a commercial electrician who has built a couple of commercial-scale clean rooms for surgical implant manufacturing facilities, so I sourced all the materials for free, I'd never recoup the cost to buy one) and never looked back, but the good ol' shmuv served me well for a long time.
Very interested in how you built/installed that induction coil. Would love to see a video on that. Great work as always!
I agree
th-cam.com/video/AOMdFAOTbmA/w-d-xo.html
YESYESYES
Yeah please make this video man I need this
I really hope to see that induction sterilizer tutorial someday! It's pretty awesome!
Very concise and informative content to a newbie trying to learn as much about this as I can. Between you, 90 second mycology, Bass drop keys and willy mico, I'll have a first full flush in no time! Much love to all you guys inspiring us just getting started!
PGT is another great TH-camr
If you have an old coffee can or something or the sort/size, you can set it on the burner on your stove for a bit to heat up and then melt the hand/arm holes into the SAB. Wont have to worry about cracking the tub either while cutting/drilling!
I did that with dog food lol 22 years ago 😅
This comment is underated
And I seen how they put the gorilla tape on each side of the hole since it's a big coffee size hole and cut an x in the tape sticky to sticky over the hole like a skirt, maybe not necessary but seems like a good design
Very nice video. Good to see someone using an SAB correctly and smoothly :)
Thanks Ed I'm glad u enjoyed it
@@MycoDogsMan, I can't even tell you how much. I would appreciate it if you would at least make a quick video about how to put that sterilizer together. I ordered the parts. I just don't really have the technical knowledge I'm not finding anything online
I'd love to see that induction sterilizer video you mentioned. Got the parts loaded into my Amazon basket, just need the guidance on how it all goes together. Thanks for your work!
that damn coil worked like MAGIC
Bro, your induction coil sterilization set up is absolutely awesome! I just got my supplies in today to make one! Thank you for sharing and linking the supplies!
I immediately ordered all the supplies. I have no idea how to put this thing together but I'm going to do my best to figure it out. I wish you would have made that video @mycodogs
I've had massive success with still air boxes. Love your vids!! ❤️
I don’t use alcohol inside the SAB anymore, because I have a strong UV-C light inside. I turn it on for 15 minutes to sterilize the inside of the SAB before I start working, and turn it off when I have to sit next to it, or else I would go blind, lol.
Then I use alcohol on everything that enters the SAB before I put it through the arm holes. Never had anything contaminated since I started doing it this way.
I even let Petri dishes harden/cool without the lids on inside the box, so I avoid condensation, and just have the UV-C light turned on the entire time the lids are off. 😊
I do as well, but you have to are sure nothing living is in the room when using the light. And I still use alcohol as a backup. I’ve never experienced contamination.
Can you point me to the lamp youre using
Super cool!!! Thank you for sharing this awesome information!!!! Would love to see a video on the induction heater.. Really beautiful rhizomorphic mycelium on that plate!!!!
Man i just love how you explain stuff
I can watch this all day
Excellent info! Just the nuts and bolts and presented in a digestible manner. Thanks for sharing your expertise/experience. Subscribed!
FYI, Those Sterlite Containers have 2 Holes at the lid level near the handles and make great connections to air tubing and air stone in water vases.
It's pretty easy to have low contamination rates with a SAB if you're already in a fairly clean environment, but only a laminar flow setup will still give you low contamination rates in less than ideal conditions as well. I rent an old house in Florida with windows that are overlapping glass strips. I can clean the house from top to bottom, run an air purifier 24/7, and have the sterile technique of an open heart surgeon and a SAB still gives me 65%+ contamination because no matter what, there's a constant supply of contamination entering the house. Nearly zero contamination with a FFU.
I would love to see a video on how to build the induction sterilizer. Great video thank you 😊
Thank you my good sir for sharing your hard earned experience with us freely! Boundless Love & Light to you❤
Man frfr, I have been a part of the community and a hobbyist mycologist for some years. But this is the thing is I have a Flow hood, also a FFU, and my old friend my Still Air Box. My SAB has been old faithful. Heck I have the FH, and my FFU. And still this I use my SAB for certain things, small jobs. Lol PLUS I HAVE HER ROCK SLAPS EVERYWHERE. I GOT MY FH COVERED IN THEM TO!!!! Man they look great. But my FFU is clean no slaps on her. But I agree 100% with everything you covered. I do certain things like you, but some other I do different. Plus you showed me some things to try out myself. Keep up the great work.
Id love to see the setup on that induction sterilizer the only diy videos I can find don’t use a pedal and I want to incorporate it for sure.
Quite a few explosions have occurred when flame sterilising needles inside still air boxes. If the alcohol vapour is in the right proportions with the air (upper/lower explosion limit), then it will ignite/explode. So, be careful and only use the minimum amount of alcohol when wiping down surfaces, and no open flames.
Loads more actually good advice you should have more views than my rookie mycologist
Yo dude! Just gotta say the advise and information you gave me when I first started has been so helpful!. I appreciate you! Borf!
you should get more views, your content is amazing!❤
fr so much awesome info
Ive been having very good results working in open air. Just shutting the bathroom door with a towel at bottom and turning on a hot shower till steam covers everything and spraying the air around and over me with alcohol and quickly bang it out. Im bout to make a diy flowhood i think just to reduce the occasional failure. The most important parts imo is making sure your grains are fully sterelized, fully colonized, and your substrate isnt to wet.👍
yea ive done transfers just on my desk and they work if ur careful
Would definitely love to see a assemble video on that induction coil fr
Definitely interested in how to build that induction coil
I'm currently building the GordoTek "flowhood" My place is pretty humid and dusty. Great video btw
Used a industrial led work light and it is connected to a remote switch so I can "no touch" operate it.
Totally interested in DIY induction coil sterilizer video. Thanking you in advance :)
This is very well thought out and described. Thanks!
This is a fantastic video, your technique is masterful
Great video
Damn that induction sterilizer looks awesome
Excellent information. Many Thanks
Right on! 🤟🏻 I appreciate content that I learn from
video tutorial for the induction sterilizer, I love it 🙏🙌
Yo im trying to get to know as mush as possible before beginning to even try to grow my own mushies and damn youre videos are amazing keep it up!!! i learned so much already
I'm a newbie. Thank you for all the knowledge.
Those induction coils are cheap if you know how to make one. Use a zvs and a coil with center tap.
Please do a tutorial on the sterilizer:)
I love your vids bro . Remember, “ignorance is bliss , knowledge is power “ - nava
Looking to get a SAB for tissue culture. I found this video super helpful. I'm very interested in the induction sterilizer. It's absolutely brilliant.
Really helpful video, thanks! Love the background music too! Subbed
Working in front of a ffu is such a QOL improvement. I hated working in a SAB
Flipping the lid over...so simple and easy, sure wish I'd seen this video when I started. Drilling holes in lids is a pain.
Where do you get rye grain berries and are they worth the expense? I've had pretty good success with popcorn.
I'm gonna make a check list using what you said it's so perfect thanks
What are the dimensions of the SAB?
The tubs that you listed are definitely not big enough.
I just don't want to waste any money getting a box that's too small and you have no link for one.
1:50 "70 bucks" said Madden xD hahaha i reconize that microscope!
Nice to see you! Peace!
Great video man, lots of good tips.
Love the patience and motivation you put out to us jumping in the community. Thank you! 🎃💪🏾
i keep my plates upside down as well while i transfer.. got the idea from you
6:40 sounds like you discovered a flash sterilization method 😂
I’m very interested in how to build the induction coil into the SAB, appreciate if you can make a video about it! Mush Love ❤️
the myco gods sent us a genius furry to teach us his secrets
This is great, thank you. Subscribed.
Wouldnt go back to a SAB after using my flowhood for the past year! Way less tams and way more convenient!
Thanks for sharing .. I like your style 😎
Very informative 👏 i will view more of your content
Even when making money doing this, a glove box can be enough of a steh·ruhl solution. But I built a flow hood because the box is annoying af.
Wish i had watched this befire ordering everytbing to build a lam flow hood. Guess ill use both methods. My first attemot at reishis.
Flow hoods are definitely a good quality of life upgrade especially if ur doing a lot of work
I have a 1000watt induction heater it only cost about 48$ years ago. So i know there are cheap ones.
Awesome video thanks my bruda!
Too cut the holes use a razor blade and vice grips and a torch.. clamp the blade at and angle away from you hest the blade till red hot and cut like butter
Having the flow hood just removes most of those prep step, it's a huge comfort increase. Of course it comes at a cost, I'd say mine cost around 300€ to build, most of that coming from the fan and filter
I'm not saying anything bad about flow hoods, they make workflow faster and easier especially at scale, just most people don't need to spend that much money especially when just getting into the hobby and if they are experiencing contams in an SAB its not because of equipment its more due to their technique and preparations
@@MycoDogs That's fair
Appreciate the video! Compact and full of information! Never the less, because we are in the topic sterile workflow, does somebody have any tips on putting the grainspawn into bulk in a monotub in the most steril way possible. Its my first time soon and i am a bit paranoid about Contamination. Obviously i dont have a flow tube neither a Still Air Box big enough to but my Monotub in.
Great video thanks
Well done! ✌
I mean it doesn't look like anything is clean in this video. Yet I trust that it is
Adding a bleach towel to the bottem of the sab works wonders
Thumbnail says "DUBUNKED" instead of "DEBUNKED". Just mentioning it if you feel like fixing it haha. Grest video, thanks a lot 👍
Thanks!!
vielen dank.
Really excellent info done well b
good stuff!
Biggest problem for me is reading to be graduations on the syringe when using a still air box
excellent info
Hi can you show how to build the induction coil? Very cool to see !!!
I have a video on it
Great video!
To put my hand holes in, I heated the top of a coffee can on the stove and melted the holes through. ..Nice and smooth with no cracks.
Thx Bloke, much appreciated. Best thing i did , was make a SAB, before i even colonized my 1st rice bag :)..Makes good sense..makes good practice. Also, tyvm for the diff tech tips, agar plates etc. Some great ideas/best practice strategies. Cya down in the Lab..BLACK TAR GANG! 😎💨💨🖤💯🤘
7:24 It is able to start the alcohol to burning isnt't it? What is visibly glowing hot that is well over 500C degree.
Thanks bro
You ever melted bag near that induction
12:16 the gloves looking all unloved 😂
Thanks!
Thanks for the super man
Diy induction sterilizer! 😮
I recently saw online that you can make a liquid culture using a spore syringe as the starter.
Def possible,But not recommended
Dude. I love you ❤️
Off topic of the video, but what type of lighting and how much for the all in one grow bags? Trying to remain reasonable on price as I am new to this. Thanks for any help and really enjoying the channel.
Scratch that. Just went to the discord channel and that was the most recent post.🤣
Built my laminar blow box for 65 bucks.
6:29 And the air sterilized suddenly. :D
Look for the video on the induction sterilizer. Id like to know how to make it. Please make a video or share the link where i can find it. Mush love
A 6 inch hole saw ran in reverse should cut a quick hole
Thank you for infos. Do you say that the liquid culture jar doesnt need a filter to breath? This means that i can make a needle biopsy, suck some sterilized liquid and air into it and mycelium can grow inside the syringe?
Noobs HATE him for this one simple trick
What if you fart in one? 😮😂
That will probably grow LBMs
Curious - I'm curious, you said at around 1:53 min that you don't encourage others to grow mushrooms for profit. Why did you say that? That was an odd comment to throw in there...
INDUCTION STERILIZER VIDEO NOW PLEASE SIR!!!!
Are you sure clean rooms aren't sterile? I'm almost certain the clean rooms at a chip fab are completely sterile.
i know they are very very very clean but I'm sure there are 1 or two stray cells floating around the room, sterile means zero life at all anywhere
What I wanna know is the song from 13:50 😂
on.soundcloud.com/KC145
I always used a ghetto-ass but very effective method known on the interwebs as a 'shmuvbox'. You tape a transparent trash bag to the outlet of a small room air filter with a UHPA cartridge, then cut armholes in the inflated bag. Bags are inherently sterile inside because of the way they're manufactured, but you can wipe it down after it's inflated for an extra bump in security. You can do anything you can do in front of a flowhood in it, and it costs very little.
I have seen this done before, I'm sure you can have success with it. There is an issue with a setup like this is the airflow inside the bag is very turbulent and aggressive, meaning if your arms or equipment have any contam on them it will get blown all over the place. A still airbox on the other hand u can rub ur hands around in dirt and work without washing them without contam because there is no turbulence
@@MycoDogs yeah, you're absolutely right. I always used veterinary gloves that went way up past my elbows and only performed one task at a time, wiping down nearly constantly. I live somewhere pretty humid, with a VERY high spore load, and have always had indoor pets and lived in old drafty farmhouses, the glovebox (still air box) was never effective for me. I understand that it's the next best from a flowhood for most folks. I eventually built a flowhood (I'm a commercial electrician who has built a couple of commercial-scale clean rooms for surgical implant manufacturing facilities, so I sourced all the materials for free, I'd never recoup the cost to buy one) and never looked back, but the good ol' shmuv served me well for a long time.
I rather have still air than cheap air