Direct ELISA | Basics Explained

In this lecture we discuss ELISA basics and Direct ELISA technique. We also discuss how ELISA Test is performed and it's procedure.
ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones.
Hope you find this video helpful. In case you have any doubts please feel free to ask in comments. I keep checking comments and reply to them.
Related Videos:
Competitive ELISA: • Competitive ELISA | Ba...
Sandwich ELISA: • Sandwich ELISA | Basic...
Indirect ELISA: • Indirect ELISA | Basic...
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[@urgyanchorol1903]

Thank you Madam.. Your way of teaching is very simple and so effective

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It always best for me the way you taught...thank u mam

[@missshamshad9626]

G

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Ma'am, i am your big fan because you teach in very simple way

[@mamtaom6098]

Tooo good mam.easy to understand.
Me aapko unacademy pr bi follow krti hu.aap bhut aacha teach krte ho mam.thank u.
god bless u mam😊😊

[@BaaYo]

Thank you 😊

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No doubt The methodology of Mam is so effective.

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[@mubashirraza850]

you are absolutely outstanding to explain all lab techniques. The reasoning of recipes is nice additions.👍👍

[@AnuragSingh-gv2yf]

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[@manbarad1364]

Your teaching technique is very interesting and easy to understand, thank you very much

[@BaaYo]

Thank YOU :)

[@hrishitheinevitable2478]

Thank you, ma'am. All of my doubts regarding ELISA is clear now. 😊😊

[@mansramalke155]

Jitni sunder ho mam utna hi sunder knowledge hai

[@healthiswealth7720]

Very good explanation. Thank you very much. I have a question, when Enzyme is anyway attached to the Antibody, why the solution is changing color only when it is bound and not changing the color when it is not bound?

[@bharatrupnar4874]

Best teacher for biology

[@Moye_moye03]

I watch ur all the lectures ma'am...u r so cute 🥰 really appreciate your teaching style ❣️ thankyou ma'am ❣️❣️❣️🙏😊🙏

[@BaaYo]

Thank you

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[@Pharmacy891]

Mam your teaching is very very thanks from my heart

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Love your teaching style and you to ❤️😘

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Very effective and smart study. I really impressed the way you teach.

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Your explanation is very good mam.. ... . And easy to understand☺️

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My all doubt are clear after watching this lesson ☺️☺️

[@dhananjaybokade4066]

Thank u mam for guide us in this lockdown...really U teach so much better then my clg. Lecturers...very helpful work form home.. Ty

[@BaaYo]

Thank you Dhananjay

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I really don't have word to express your way of teaching.... It's really well and I'm students of p.hd microbiology and teacher for(m.sc) microbiology as well

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Iske liye bahut-bahut
dhanyawad

[@dhrubajyoti_myself]

I was searching for this concept and you gave me the best. Thank you so much

[@shireennaz736]

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My favourite learning channel

[@SatishKumar-sm3zp]

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[@AnilKumar-hv9ej]

Nice mam
Ek ek chij ko bahut achhe se smjhaya🙏

[@priyamusale4098]

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[@priyajindal3055]

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[@harshsoni674]

Hiiiiiii

[@dakshapatel2455]

Can u plzz upload video related to ...how ELISA technique is used to find plant disease with example

[@fahmidabanu1686]

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[@sajidabanoo638]

Thnk u mam. Give me information about serum plz mam your teaching way is very simple.

[@Dr.Aqiikhan]

Great work mam
Allah apko khosh rakay

[@Adventurewithsudipmampi]

Cuteeeee khub vlo

[@AnjaliNautiyal271]

Mam enzymes are attached to antibody by which bond ???? Plz tell me

[@rishvkumarrishvkumar1164]

Mam aap ka lecture bahot hi amazing hai please mam aap AMES test ka bhi video upload kijiye february ko university exam hai mam hame aapse bahot hi umid hai please reply dijiyega

[@mohammadmasroorzafar9728]

Can you add the screen shot of table in which colour produced and enzyme used was mentioned?
Was not clear in the video

[@AjayKumar-uy3de]

Your videos are very helpful
Thank you ma'am 😊🙏

[@bharatnegi3088]

Thanku so much for this topic 💓

[@prakashpandey6977]

Mam direct elisa me hm log antibody ko toh human serum se nikale the...then wo enzyme linked kaise hua...?? Microtiter me dalne se pehle?

[@shyamaprasadghosh4859]

Ma'am, if the antibodies don't bind with the antigens then how will they give false colour change? There should be no colour change in case of no binding?

[@CHAMPIONSBio]

Mam in direct Elisa we find antigen but in diagram the antigen is already fixed . According to this we can only find antibody .....? Pls explain mam ...

[@anmolgulati27]

You lectures of simple and self explanatory flowchart help me to make my notes in very effective manner thank you so much may god bless you ma'am 🙏😊🌅

[@bhagyashreekanani3802]

Easy to understand mam thank you so much mamm
Aesihi video banate rahna.

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Hi .

[@harshsoni674]

Hiiii

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My name is harsh soni

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Kya Aap mere friends banoge kya

[@harshsoni674]

Please

[@WaseemAnsari-mj1bo]

Mashallah bohat acha lecture h

[@satviksharma9843]

Please make video on enzyme immbolization

[@MuhammadRashid-nr5bh]

Very nice teaching method 🌹

[@sanjaytiwari4202]

Woow you tech quite gentle way thxxx a loooot dear madam

[@mohdfiroz9563]

Thank you mam very simple and effective

[@anshikamittal7548]

Ma'am can we use all four methods to detect antigen and antibody both?

[@jyotigalyan9188]

Easy to understand mam 👍😊
Plz make video on radioimmunoassay

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Shukriya lecture bht acha hai

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U r a gem with incredible teaching skills.

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hello mam
kya apko lagta hai ki main safe hu
1- hiv rna pcr quantitaive 34 day is TND
2- ANTIBODY AND CD4 TEST -negetive and normal
3- cbc .report normal 2 time (one month )
4- combo test after 8 week comes negetive
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(still not symtoms but feeling illness due to
to Anxiety , and Acid reflux

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More informative videos
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Keep watching

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Ma'am,you are always Best.

[@evelingeorge7834]

Very useful...mam can u please make video on enzyme therapy using streptokinase?

[@muhammadkarim7358]

Brilliant lecture.
You are very much welcome

[@BaaYo]

Thanks

[@muhammadkarim7358]

Lovely regards

[@aqsarani7841]

Ma'am
can u upload vedio on cell culture?

[@BiobyNishit]

far more better than shomu's biology, thanks

[@BaaYo]

Thank you

[@ramchandrasuthar2848]

GREAT ANALYSIS MAM

[@ShaliniPrajapati-nx4xq]

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Can u please make a video on HighThroughput sequencing .

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I always wait yr video

[@Adventurewithsudipmampi]

Mam I lve u and ur teaching

[@MuhammadFarhan-wo3ij]

when the sample is added in direct ELISA?

[@sandeepkalita7125]

Great explaination, Ma'am.

[@ManishYadav-id7wl]

Hello Mam this make me CONFUSED so much I don't know answer of it please tell
IT'S MULTIPLE CHOICE QUESTIONS
Which of the following descriptions are correct about "cross reaction"?
A
Cross reaction means that a specific A antibody can react with different antigens;
B
Cross reaction means a specific antigen can be recognized by different anitbodies;
C
Cross reaction means that different antigens have the same epitopes
D
It is a cross reaction when anti- cowpox antibody can neutralize with the smallpox virus
E
It is a cross reaction when anti- E cowpox antibody can neutralize with the cowpox vaccine

[@Biotechie_Concepts]

Maam why Only Colour changes When the antibody attaches with antigen?
Normally also enzyme can produce substrate then what is the logic behind Antibody should attach with antigen then only it's leads to formation of product which produces colour change?

[@anumbasit4185]

Soo much helpful✔️

[@Feel-m3n]

Welldone. . .

[@ArchanaSingh-ld6cy]

Thank you ma'am. This video was really helpful.

[@tripathyseema]

Nicely explained

[@ketanmakwana5300]

Nice info 👍

[@virajdayama]

Very nice 👍🏻 Good luck Arti

[@SimranKumari-bc1zm]

Can you make a video on immuno PCR !?

[@ahatsham87]

Ya antigen kaha sa laya mam . Serum se hma antibody hogi na

[@muhadsaghaffar746]

Excellent

[@akshaygurav2806]

Mam please biosimilar molecule ke bare me bhi lecture upload karo, sath me ELISPOT technique bhi.

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Baayo ka kya mtlb hai mam ❤

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Nothing... bio naam se ban nhi rha tha to baayo kr diya...

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Superb mam❤

[@lisaxo2537]

Direct Indirect Elisa is used to detect Antibodies

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Very well explained.👍

[@harerammishra3388]

Hlw mam
I wonder
When listening your lacture

[@MLTWithMianIhsan]

Jab ham direct elisa mai
Example Hbs ag antigen apply karty hai microwell mai to es hbs Ag ke elawa or Buhat ziada antigen present hai serum mai wo Q bind nahi hoti.

[@rupaldesai1281]

Nice explanation!!!

[@Ishika976]

Mam direct elisa mei micro well coated with what??
We find in patient serum have any antigen molecule.

[@Aishatistic]

Well explained

[@Random-bq7yo]

Impressive

[@swagatikapradhan961]

Ma'am make a video on RIA & bioluminescence assay

[@mevankumar]

Thanks ma'am good way👌💙

[@hareshdubey2664]

Dhanyanbaad mam

[@adilfarooq6494]

you are Great God Bless You always

[@sapnakant9725]

What's name of primary antibody?that Use in indirect ELISA.