How a gene gun works

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  • เผยแพร่เมื่อ 13 ก.ย. 2024
  • Kyle Taylor from the Glowing Plant project explains how a gene gun, which is used to transform cells with synthetic DNA, works.

ความคิดเห็น • 56

  • @mewantbrains
    @mewantbrains 9 ปีที่แล้ว +44

    I believe in the video you said you will demonstrate it firing
    I don't see that anywhere in the video

    • @DJMouthwash
      @DJMouthwash 9 ปีที่แล้ว +1

      ***** 0:28 seconds.

    • @mewantbrains
      @mewantbrains 9 ปีที่แล้ว +1

      That's where he says he will demonstrate the firing process but it did not actually fire

    • @TAXABiotechnologies
      @TAXABiotechnologies  8 ปีที่แล้ว +11

      It does fire, it's just not that dramatic!

    • @ashdroid
      @ashdroid 7 ปีที่แล้ว

      The machine is turned off and not running at that specific time 0:28, it even has the door open.
      EDIT: I just saw your other video.

    • @abefromansausagekingofchic6480
      @abefromansausagekingofchic6480 6 ปีที่แล้ว +1

      Riddlemethis yea I was kind of blue balled too. Found the other video though

  • @JulianoVitiello
    @JulianoVitiello 11 ปีที่แล้ว +7

    Here is a good tip: wrap electric tape on the top part where you screw in the rupture discs. That way it wont sound like nails on a chalkboard each time.

  • @lakshayaindrapugal542
    @lakshayaindrapugal542 ปีที่แล้ว +7

    Thank you soo much. Actually I was thinking how does this thing work?. It was referred as " bombarded " in my book 😂.

  • @birk_lab4805
    @birk_lab4805 5 ปีที่แล้ว +56

    You stopped before the bombardment! WHHHHYYYYY??? :,,-(

    • @realryder2626
      @realryder2626 ปีที่แล้ว +1

      Obviously he got a txt and had to reply

  • @DipanshuGhosh
    @DipanshuGhosh 4 ปีที่แล้ว +21

    You should have demonstrated the whole process. You didn't fire the nano particle coated with preferred DNA.

    • @lolpotato
      @lolpotato ปีที่แล้ว +4

      Generally gold or tungsten with attached desired gene is used to fire into the vector. This is what i have been taught but never saw it in practical

    • @Noveshd
      @Noveshd ปีที่แล้ว

      ​@@lolpotato yep its only done when u choose biotechnology as career.....not in medical also

  • @callahancovington4278
    @callahancovington4278 8 ปีที่แล้ว +9

    It would be nice if there was a laser to help you align the tissue directly into the gun's trajectory.

  • @adventuresoflife486
    @adventuresoflife486 2 ปีที่แล้ว +3

    If there is no vector present we use Gene gun/bolistic for plant and microinjection for amimal... 🌷❤️❤️

  • @tahiruibrahim7012
    @tahiruibrahim7012 9 วันที่ผ่านมา

    I have a lot on my mind to achieve in this field, just hoping one day I will get the opportunity to join a lab acquire knowledge and bring it out to solve those problems 🙏🏾

  • @blazearmoru
    @blazearmoru 11 ปีที่แล้ว +3

    aww. you didn't show us the process with the onion D:
    i hope it's in the next video ^~^

  • @new_contents_all_day
    @new_contents_all_day 6 ปีที่แล้ว +1

    Can u combine mamels with plants

  • @jazzmessenger8
    @jazzmessenger8 10 ปีที่แล้ว +2

    What genes were on the tungsten?

    • @jayesper4390
      @jayesper4390 6 ปีที่แล้ว

      Bill Ricci Dunno, but gold's the way to go. Tungsten should be out of the question.

  • @kellycarpenter9350
    @kellycarpenter9350 3 ปีที่แล้ว

    Hey I would like to learn how to inject flavonoids into plant tissue to create flaver into plants .like strawberries..I want to cross flaver with pink pepper etc. Kel

  • @Ekergaard
    @Ekergaard 11 ปีที่แล้ว

    I have heard a Ph.D. student say: "This is our nanodrop there're many like it but this one is our". So it's temting to ask if anyone has said: "this is our gene gun ..."

  • @PrasetyoMuhammadDwiBiology
    @PrasetyoMuhammadDwiBiology 7 ปีที่แล้ว +12

    real men use heatshock :D

  • @KaitokiNohara
    @KaitokiNohara 4 ปีที่แล้ว

    Now I know how it works

  • @sapnamenghwar8219
    @sapnamenghwar8219 ปีที่แล้ว

    Oh I was waiting for them to bombard the tissue, just to see the machine in action 🤔

  • @FoxCurtailed
    @FoxCurtailed 11 ปีที่แล้ว +1

    It looks like a tiny, old school gas pump.

  • @NavinJohnson_thethird
    @NavinJohnson_thethird 3 ปีที่แล้ว

    Dr. John Sanford waa the primary inventor of the gene gun. A former Atheist who became a Christian.

    • @David-ck3gv
      @David-ck3gv 2 ปีที่แล้ว

      The prototype was also just a .22 caliber that shot blanks into plant tissue lol

  • @axanachernyavskaya3556
    @axanachernyavskaya3556 10 ปีที่แล้ว

    thanks a lot! very useful!

  • @deborahjudson350
    @deborahjudson350 7 ปีที่แล้ว +1

    It does not ever fire

  • @zombiebillofthedead
    @zombiebillofthedead 11 ปีที่แล้ว +1

    Filming on a phone I see, wouldn't have known if the picture didn't shake and make that vibrating phone sound. Not that it matters.

  • @christurnblom4825
    @christurnblom4825 6 ปีที่แล้ว +5

    politics of gmo vs non gmo aside, I find it humorous that I've actually heard a scientist on the radio say that this is no different than what farmers have done for thousands of years when breading plants for a desired outcome. This, in response to being asked what he has to say to people who are concerned about the safety of gmo's. They weren't taking calls but if they had been my question would have been "What force of nature causes a similar effect to a gene gun and achieves the same result?"
    Whether or not there is net harm to the environment aside, I just found it insulting that he would play on people's ignorance like that.

    • @christurnblom4825
      @christurnblom4825 6 ปีที่แล้ว +1

      So one of those you mentioned, blasts the same "clip" of DNA into other DNA at over 600 mph, blasting apart the DNA while getting random pieces to stick in the desired place? I guess I do have a lot to learn.

    • @christurnblom4825
      @christurnblom4825 6 ปีที่แล้ว

      What are you talking about? Sarcasm is great! Example:
      Damn you for knowing stuff & making me think about what I've said!!
      Sarcasm helps me feel like I'm doing well while your schooling me. ...and it's occasionally funny.
      All I got left is the thing I put aside at first ie my concern that being selective in that process has a different outcome than in nature or even when we selectively breed in the old method because we don't have all the other iterations that would result, out their doing their thing. I doubt we have any tech. that can figure out if those two ways are any more or less harmful to the environment than the gene gun method but my hunch is that it probably puts things out of balance. At the same time, I'm pretty sure that nature will just shake us off or swat us with her tail and go back into balance if we become too much of a problem.

    • @FringeWizard2
      @FringeWizard2 6 ปีที่แล้ว +2

      I don't think there is a problem with genetically modifying organisms but the specific traits they choose to develop in plants are often really stupid traits they should not have aimed for, whether it be through selective breeding or GMO. Stuff like tomatos that produce more crop with the same amount of nutrients; the result is tomatoes that have significantly less nutritional value. GMOs plants the produce their own pesticide are also a stupid "innovation" as pesticides are often (not always) not very good and harm our health. When they try to develop plants with commercial interests in mind it often times has a lot of drawbacks. It's not also just GMO that is bad but many varieties of plant which are grown for commercial production are in my opinion inferior to heirloom varieties developed by gardeners who just wanted a tasty or beautiful tomato.

    • @selenaburke2956
      @selenaburke2956 6 ปีที่แล้ว

      There are foods you eat with transgenes that got there naturally. like potatoes

    • @MinxInSinx
      @MinxInSinx 2 ปีที่แล้ว

      @@FringeWizard2 do you think it is ok to genetically modify your genetics & the lineages of your offspring?
      The only way for your dna to change is if you inject it into the user.
      We are what we eat

  • @ducktheduck9792
    @ducktheduck9792 3 ปีที่แล้ว +3

    kuku

  • @بنتالانبار-ن4ث
    @بنتالانبار-ن4ث 3 ปีที่แล้ว

    الي ما يعرف انكلش شلون يتابعة🥺

    • @balbaks
      @balbaks 3 ปีที่แล้ว

      بتقدري تدوسي على علامة CC وبعدين روحي اعدادات الفيديو > الترجمات > ترجمة تلقائية، هي فيها شوية تخبيص بس راح تساعد بالفهم

    • @بنتالانبار-ن4ث
      @بنتالانبار-ن4ث 3 ปีที่แล้ว

      @@balbaks تطلعلي كتابه انكلش ما يصير عربي ؟

    • @balbaks
      @balbaks 3 ปีที่แล้ว

      @@بنتالانبار-ن4ث لا موجود عربي بس ترجمة تلقائية
      بعد ما تدوسي على CC روحي لاعدادات الفيديو (إشارة المسنن) واختاري ترجمة تلقائية
      أنا فاتح من الحاسوب ممكن من الهاتف يكون مختلف

    • @بنتالانبار-ن4ث
      @بنتالانبار-ن4ث 3 ปีที่แล้ว

      @@balbaks يمكن الموبايل يختلف راح اجرب الحاسوب شكرا جزيلا الك

  • @biologyinhindiandenglish8787
    @biologyinhindiandenglish8787 4 ปีที่แล้ว

    Ho sakta you can follow any responses to you and

  • @smilesinger3652
    @smilesinger3652 3 ปีที่แล้ว +1

    Guys who are from vyshavi college 😀

  • @anusandhanwithdevantiwari181
    @anusandhanwithdevantiwari181 4 ปีที่แล้ว

    Can u tell me hindi

  • @timdwyer3516
    @timdwyer3516 6 ปีที่แล้ว

    Anyone here from 7/8 b

  • @ឃុនលួង-ហ3ខ
    @ឃុនលួង-ហ3ខ ปีที่แล้ว

    PDS1000$$

  • @miguelpatrao8999
    @miguelpatrao8999 2 ปีที่แล้ว

    ahhhhhhhhhhh cromo