A Grape Made of... Meat?? - Tissue Recellularization
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Decellularization is a pretty strange process. Using a special type of soap, it's possible to remove all the cellular material from a piece of tissue, leaving only the connective proteins and polymers behind that normally hold the cells in place. It leaves you with something that looks like the starting material, but is really just an empty sponge called a scaffold.
Doctors and scientists have been exploring this technique for many years because those empty scaffolds are extremely useful for cell culture. By seeding them with cells taken from a patient, it's possible to grow a brand new organ or tissue able to be transplanted into a patient, potentially saving their life. The same technique could even be used to make lab grown meat.
Today we explore this amazing process and turn an ordinary grape, into meat.
More reading:
Review paper - onlinelibrary.wiley.com/doi/p...
Andrew Pelling papers - journals.plos.org/plosone/art...
pubs.acs.org/doi/abs/10.1021/...
Gabriels stuff:
www.scihouse.space/
/ glimsd
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_____________________________________________________ - วิทยาศาสตร์และเทคโนโลยี
The era of plant-based meat is over. The era of meat-based plant has begun
LET'S FUCKING GOOOOOO
@@rojoscostanada8685 LETS GOOO
WOOOOH
YASSSS!!!
WHY YALL HAPPY THEY GONNA TAKE OVER THE WORLD
I like how this seems to have two applications:
1. Organ transplants
2. mmmm meat berry
mmmm meat berry
Mmmm me gusta
mmmm meat berry
Mmmm me gusta
mmmm meat berry
the idea that lab-grown organs can exist within my lifetime is insane. it's something my family has been hoping for since my brother got his kidney transplant
I'll eat his other kidney
Yes that's a threat
@@TylerTMG I'll defeat you before you can do that.
@@pinkfurret6245 nice pfp btw
@@Prime501 cool thanks
I like how he asks, "Why don't we make a meat berry?" with the candour of asking, "Why don't we get some Macdonald's icecream?"
Is there even any ice or cream in that stuff?
both difficult
2018: They did surgery on a grape
2019: They did cell replacement therapy on a grape
2020: We have become the grape
Detroit: Become frutal.
3020: Grapekind gain rights to vote
3021: All of humanity is destroyed. The grapes have created human death camps and eliminated all members of our species. They fucking won. Grapes now rule the world.
Yes but the surgery was from a few more years back
draconiusultamius I was waiting for a comment like this😂😂👍🏾
2018: they did surgery on a grape
2019: they did surgery with a grape
big boi angle 2020: they got surgery from a grape
2021 grapes have defeated apes...
2009: "do you have any grapes?"
2022 grapeocalypse...
progress
I remember my friend said that he could turn a grape into an onion, he just peeled the skin of and I believed it was an onion.
I’m sorry what
bro were you 8 months old
Holy crap that's hilarious and also pretty disturbing, wtf
So grapes are ogres. Makes sense
Yeah my dad did that with his face once. Peeled it off and then suddenly he was my mom.
Ever since we have had no milk at home.
The full video you made three years ago was actually what inspired me to study biology! And this semester I'm going to write my bachelor thesis :) thanks for that, you are awesome!
hey man hows your thesis going? did you already finish?
@@dedusmuln Yes! I got my certification in September
@@flamezero.x2957good shit man
2025: The grape did surgery on me
You forgot “in Russia”
@@idioticbosd7995 IN SOVIET RUSSIA GRAPES DOES SURGERY ON YOU
Quoc anh Vu Pham yes
If we make it past 2020😥
Well you should thank him grapes are putting their lives on the line to save humans.
Doctor: Congratulations we found a heart to replace yours!
Patient: Oh, well who's the donor?
Doctor: Grape
G r a p e
patient: aw man, I was hoping for apple
@@omnical6135 no, only grape
@@insertcoolnamehere937 can I choose what kind of grape
@@josephdavison4189 no just grape
It's amazing that I read a scifi comic that's at least 20 years old by now where they had this exact concept of decelluarising an entire human and fill him back up with new cells to extend their lifespan to several centuries.
Idk why but thus is funny to me
That's an entirely different human, at that point...
I guess it'd have to be genetically identical stem cells...
From "they did surgery on a grape" to "the made a meat grape". Amazing
"I need suction, we're going to lose this grape if we can't find the bleed and seal it! Get me another bag of Type G blood, stat!"
So Groot is basically a decellularized human with plant cells.
@@DShKa. there's only one way to find out :)
Yes... only one way to find out..
CUT OPEN HIS BODY AND SEE WHATS INSIDE!
That’s one way to put it
Yes but why?
no hes a completely different race
Breaking news:
Scientists just pulled the UNO reverse card on vegans
Lmaooo
lol
Meat based plant
Given the ingredients you need to create this thing I doubt vegans would eat it lol
Carnivore diet from plants o0o
Given your title this is NOT what I was expecting. You’re a fantastic speaker, and this script, it’s at a peak of greatness. I love this. I learned something today. Thanks a lot man.
Patient: *lost a testicle*
Doctor: *puts a grape instead*
2018: "They did surgery on a grape"
2019: "They turned a grape into a monkey kidney"
@pulpnonfiction bruh
@pulpnonfiction I mean, to be honest, Quake was a good game.....
ok boomer
@Nick Gerr bruh
SrLupinotuum ok boomer
"Nice ear"
"Thanks, they're from Apple"
Wait for it... this comment is gonna get lots of likes and replies if we wait long enough when people say it’s underrated
I'm more scared that someone complemented your *EAR*
I-ear
Nice earphones
Randome person walking past: love your ear shape
Embedding tissues in paraffin could enhance the quality of the slices for microscopic visualization. I'm very interested in seeing comparative images of grape tissue, hollow grape cellulose, and meat berry tissue prepared using this method. The results must be fascinating. Although I found a video on this topic informative, I'd appreciate more detailed visual comparisons.
1:03 Ah crap.. I forgot to water my liver…
I guess that would make it a Liverpool
@@jackpaxton9391i laughed 😂
"can I have some boneless grapes?"
"no, unfortunately we've ran out of those. Would you like some seedless meat instead?"
LOL
This is a conversation that is going to happen in the future.
Yes, screenshot this to post on aged like wine when it happens. And don't blur my name.
lol true
Its gonna happen
sure I want seedless meat
The chemical “gently” disintegrates every single one of the cells.
@Dukaag Cola screaming gently, of course.
@Dukaag Cola Every night I gently and peacefully cover the grapes with acid and fall asleep with ease.
not only that, SLS is a common ingredient in shampoos, which is why it's known to damage hair / scalp cells
@Katie Greenstein new shampoo not only provides a deep clean but also gently liberates your cells from your weak useless human body
I wanna be gently disintegrated 😩
That ear looks so much better than when one was growing on the back of a mouse!
This was in my recommendation for quite a while and now that I have finally watched this, I feel BIG BRAINED.
the fact this kind/quality of content is completely free on yt is insane to me.
mad props to the educational section of yt.
Consider becoming a patron, biology is expensive and this video doesn't have ads paying him for it.
Google agrees, that's why they paid respect to educational YT channels in the rewind by doing a "science experiment".
Alex Hamon> this video doesn't have ads paying him for it
Yes, it does, it's just that even without an ad-blocker, ads don't always run on videos, sometimes they do, sometimes they don't.
@@user-vn7ce5ig1z
If you see yellow marker dots in the bottom timeline, then it has ads. This video doesnt. Anyway it is sponsored by Nord VPN.
Kketansa Art
Only if there are adverts running on the video at the time, and only on TH-cam desktop site. Anyway, instead of arguing about it, there's a ko-fi link in the description, so why not donate and claim the moral high ground of not expecting a reward?
"Yellow means VERY bad!" Apparently this is consistent across the sciences, it's not just chemistry.
Snow too
@@skanderman8769 😂😂
@@skanderman8769 God tier comment 🤣
@@Sockfish-jj6on this isn't reddit
So banana is bad?
Just watched this from my watch later playlist. This is so cool and mindblowing, motivates me to join the microbiology/biotechnology
This is so mindblowingly awesome!
Thank you for this!
I came here for meat berries and I came out actually educated on cells growing and organs and I feel smart
Peter Parker your username makes this so much funnier
Now i can grow a beeftree
@@averypleasedbakedpotato8609 dude can i have one?
thank you peter parker, very cool
Name Peter Parker profile picture mii bob ross so I am confusion
I love when smart people do cursed shit like this, simply because its possible.
*yes*
Cursed? More like the blessing of science.
Everything science doing is literally miracle.
Having a pig heart as replacement sounds cursed to me, cool science regardless 😂
This is literally my BFF talking about me. I do cursed shit on a daily basis and prank her with it
Wouldn't it make more sense to, in addition to staining the meat berry slices properly with the correct type of stain, maybe try fixing it in formalin first before slicing for a better slice. Or use the same technique used for a frozen section in an operating room, as formalin may not work well given the mechanism of action is essentially crosslinking proteins and cellulose is, well, obviously not a protein. But a frozen section methodology would likely do a decent enough job of fixing it before slicing, such that it doesn't just disintegrate into mush
Suggestion here! Why not try using unripe jackfruit as scaffolding? Since jackfruit flesh has this very fibrous texture that it's used in my country, the Philippines, as a meat substitute in a dish.
Just imagine telling someone, "my ears were once apples"...
wouldn't you say "my ears ARE apples"?
@@guh2908 no because I ate your ears
😂
Cauliflower ear can take a whole other meaning now
@The guy you see in a mirror kommunist
world in 10 years:
-meatberry steaks only 2.99$
Original old style cow steak only 49.99$
Still, meatberry sounds tasty and cute at the same time. With just a hint of nasty😅
Ngl 'Meatberry Steak' sounds really nice as a name
@Electric Pheonix stfu its a joke
@Electric Pheonix not exactly, as when scientists perfect the science they'll be able to do it globally, easily to the point you could almost make it in your kitchen, drastically dropping the price of foods as we could mass replicate them. this could be true, or heck, it could be pennies!
This is like a horror movie concept, once we discover how to make functional organs out of plants, we will consume every single piece of biomass in the world until there's nothing left-and that sounded waaaaay better in my head, DANG IT
So you're saying we will harvest earth into a void?
"the shells become fragile" - proceeds to pour water from a gallon bottle directly on them - XD
Noone’s gonna talk about how he made this a full blown tutorial
WaTeR LeMoN and? I’m gonna make this during the summer holidays.
@@kingjdog tell us how it tastes
thats the point
Jashak You think the average viewer has access to equipment to make these?
They also teach how to design your own GMOs.
2010 Me thinking the future is going to be full of cyborgs.
2020 Me realizing the future is gonna be full of fruitborgs.
@Hussam Almahdwai you still understood what he meant so it made sense
@Hussam Almahdwai wow dude he was joking chill out
Creepy confusion
Oh CrAp ThEyVe EsCaPeD
People are fruits
Watching this 3 years ago and I don't know a single thing because I don't know English and a lot about science. Watching this now amazes me how simple they explain things.
Yep. It’s possible to build structures as long as you have a good scaffold, which is the ECM. Ultra high resolution 3d printing will be instrumental here.
“Yo why u carving that apple”
“Oh, I’m just making someone’s future ear”
“You’re WHAT”
I ear you
A sentence I never thought I'd hear
That’s not how it works
Reality Rupture BuT you didn’t
@@felix.mp3639 they read it. XD
1950: "we will have flying cars in the future"
2019: meat grapes and apple ears
Still cool so. :)
Don't forget mushroom furniture.
and flying cars
Delicious.
Better than flying cars which was a stupid idea to begin with.
Today I went to the doctor where I had a medical procedure done and I was in a lot of pain trying to recover. I started talking casually with a nurse to help distract myself from the pain and I vaguely remember that I introduced the concept of meat berries to her. She said she is now going to watch your TH-cam channel. I don’t fully remember what happened but I think it went well.
You can use fluorescent nuclear stains instead like DAPI or Hoechst 33342 to pinpoint where the cells are at least (by staining their nuclei). You can also use Phalloidin conjugated with fluorescent probes (more costly) to stain the actin and you can visually see the cell body. Lastly you can use lipid-friendly (lipophilic) stains like DiO or DiI to stain the cell membranes of your cells first before injecting them to the grape matrix. That way you can tell apart the grape parts form the cells.
2 Kinds of people truly impress me:
1) Musicians.
2) Crazy science people.
Woah there, I'm both of those! Are you proud of me
@@CedarTallman musician is viable I started making music digitally since I was 12
@@clakscovsky same-
@@CedarTallman HEY IM AN ORCHESTRA KID AND A LAB ASSISTANT
@@clakscovsky so you aren't a "crazy science person"
2094: "See that dude over there?" "His body is made out of apples."
the iperson
lol
LOL
"Oh really? My niece was an apple as well!"
Honestly that would be so funny and so cool
5:25 thanks for the advice sir
I was really worried when my sds solution wasn't dissolving
Btw I was making a finger for my dog in the backyard
Me too
I don’t understand anything he’s talking about but it’s still so interesting to watch
I thought this would just be a meme but I actually learned something
[REDACTED] then it's memeing time
@[REDACTED]_ I'm you but better.
@[REDACTED]_ *engineer ho down on you*
How would it be a meme?
This brings "We did surgery on a grape" to a whole new level.
Stfu
TKOR?
@@xtcrider8270 chill
THAT IS WHAT I THOUGHT
“We made a grape perform surgery on a grape.”
I feel like my dad saying this, but that lil handheld vacuum gadget is nifty as hell
Those shabriri grapes from elden ring suddenly feel so much more plausible
Them: *puts animal cells in grape meat*
Vegans: wait, thats illegal
*toxic vegans
PETA: WRITE THAT DOWN
Tshadowangel no. It’s a joke bro
This isn’t reddit *toxic vegans
😂😂😂
de-cellularization is literally just removing in-game texture files on an object in real-time
Pallet swapped grape, or meat berry. You decide!
Fun perspective
This is why I’m a crustacean main. Only octopuses have texture changers, so I don’t have to worry about it too much in the newer Southern Ocean server.
Max Crustacean mains no skill, Cephalopod mains have high skill floor
cephalopod mains ftw
😂 texture packs
wow, my mind blown. keep this science going!
That process is exceedingly simple. It's basically just growing animal cells with a few extra steps.
I know this from my laboratory assistant education.
“Hey, i’m buying grapes what color you want? Purple or gre-“
“Meat.”
M e a t
M E A T
M e a t
M e a t
M e A t
Are you winning son?
Yeah dad I'm watching a man make meat out of grapes.
It’s kiwami japan all over again
Xx Selena xX even more quality content
grapes out of meat*
400th like
hope i made your day better
It's missing lamb sauce... Don't tell him until we find it.
Cool you’re using gibco products. I worked for them in the early 00s in quality and they make good product, near Buffalo. Bought by life tech and owned now by Invitrogen last I knew. Glad to see it’s still going strong
If you put two similar types of cells into the structure, could this be used to create cross-bred plants?
I clicked on this just 'cause I wanted to watch a cursed video and by the time it ended I had a medical license.
Zech underrated comment lmao
Lol
This is how you impress your parents who have high expectations
yah like we have a lab
"I picked grapes because it's the ultimate punchline for my joke" Just love it, seriously, I love that mentality.
Is there anyway you could do this in the opposite way like replace the cells in sandwich meat with berry or plant cells??? That would be interesting.
They don’t have the same scaffolding as a grape or a heart and it’s generally ground up but I would think there’s a possibility
@@TheBaumcm Perhaps if it wasn't ground up... lol
This is crazy. Crazily cool!
Imagine going to a restaurant and asking for
*MEAT GRAPE*
Meat berry
G I V E M E M E A T G R A P E
I can only dream...
Useless Shroob it’ll happen. Trust me.
Popcorn chicken
This started out looking like something you could do at home
I was so hopefull
Dukaag Cola no, i dont think so.
You can make a centrifuge with string and a disk. Not sure how to get your hands on all the ingredients, but I bet you could if you searched enough.
Christopher Thompson yeah, not so sure about the water insulated incubator and the fetus juice
Exactly what I thought! :D Grapes? Check. Scalpels? Obtainable. SDS? Never heard of, but I'm gonna check on eBay. And slowly it went into bottles and plates I've never seen.
ooooommmmmmmggggg so detailed so many chemicals contradicting each other 😮😮😮😮
Never knew that SDS/SLS was used for decellularization. Explains why shampoos and soaps that contain it tend to be the ones most people are sensitive to-I myself am highly reactive to it.
A man underwent a pig heart transplant, they didn't expect him to survive the surgery, but he actually lived two months longer than he would've. Not perfected, but they're making a LOT of progress compared to where they were when this video was made
Okay but imagine living with a pig heart. Wouldn’t that just be so odd?
Aside from that, that’s really cool!
@@epicjay8615 it would be a bit wild to be going about your day normally iust to remember "oh huh, the heart pumping in my body rn once belonged to a swine"
baby kitten heart, 2x the beats
and they probably learnt a LOT from that experiment
@@darkmoondeath00 You're weird
at first i was like "moral baggage" is a weird way to say "ethics" but then i saw you were making meat grapes out of liquidized cow fetuses and monkey kidneys and i understood
I mean-
Love this man so much I've watched almost every vid... I want to meet this man in Real life I would practically Kms to meet him and just see how he works this..
woah what an explanation!
"how do you like your grapes?" "oh i like them meat"
catgrain animations meatium rare
Imagine being they guy who says “I have the heart of a pig”
Inevitably, someone's going to figure out how to build and plug in a heart based on a lion's heart, just for novelty. Even if it requires a fair bit of over/underclocking the thing or changing the channel diameters to get it to roughly match human spec.
Imagine being the guy who says "I have the heart of a berry"
My friend name is Anne Leonhardt AND yes she's from attack on titan lol
Where you on my discord?-
I know a guy with a pig heart. He has had one since he was a child.
I removed cells from mashing up strawberries and mixing it with liquids in year 10 science class. I don’t remember how cause I’m not good at science so it was more of just a fun group project but it was still cool to see an actual cell
crazy work keep it up man!
Interviewer: so what makes you so special?
Me: my ear is made out of apple
Link da Man Sponsored by Apple Biotech Earphone
It's a nice snack.
@@ZodiacBlack11 you can connect earphones to it, only airpods though, technically you can't use anything else
Only airpods
Why did I say this? It's just like if in the future apple cibernitized everyone making them apple cyborgs
The equivalent of this video.
Now thats a whole new level of kink
Vegans: “Eat vegetables and fruit instead of animals!”
Scientists: “Yesn’t”
Lol
Shut up you veganistic statistic lover
@@coolxg4357
Woah now, where did this come from
@@coolxg4357 Woah woah woah! Vegans are OK, but Vegans that acts like a d*** head to non vegans is NOT Ok.
No I’m pretty sure that’s the vegan teacher
the soap that they are using ( sodium lauryl sulfate ) is also in most toothpastes resulting in a bitter flavor when eating say an orange after brushing your teeth
sodium lauryl sulfate is also found in some kinds of toothpaste and might give the grapes canker sores, like on my lips.
broke: vegan hamburger
woke: non-vegan grape
Coke: we have weed in our stuff :)
Joke: *am I a joke to you*
*:3*
Amelia Price oo snizzle
Technically the grape should be vegan as even though it's meat, it's not meat that was harvested from an animal but grown in a lab, which is how lab grown meat can be labelled as vegan even though it is actual meat. Veganism by definition isn't about the meat, it's about where the meat comes from.
Endo Heart Cookie it was cocaine not weed, it’s in the name.
Endo Heart Cookie ew
Love how the detail of the video is as if I had the expertise and the equipment to do this at home.
Gameryusic 825 what? You don’t have a fully teched out Dexter’s Laboratory style lab hiding behind your walls like the rest of us? Sucks to be you, friendo. Sucks to be you.
Well,now you have a shopping list. Seems doable
Some Five Minute Crafts type shit, no biggie
Seems like the video was designed to make us feel "close" to the whole concept of lab grown food. Like it's a focus test for the industry to examine how much they can get away with.
I wouldn't be surprised if they use this technology to convert garbage into food so the upper class can sustain their own standards. Soylent Green anyone?
How large a scaffold can this technique use? I'm thinking that if you were using this to make lab-grown meat and used, say, a watermelon (once you dealt with the seed issue) the quantity wouldn't be as much of a problem.
PS apparently one of the main ethical roadblocks with lab-grown meat is that the petri dish technique requires a cow's uterine fluid (so in other words you have to kill calf foetuses to get it), does FBS avoid that matter if it's derived from blood plasma?
I'm fascinated and grossed out at the same time.
" Vegetarians developing trust issue with Grapes"
Lmao 😂😂😂
ahahaha
🤣🤣🤣
@@a1.onnat_ silence
@@a1.onnat_ no
im in tears
1970: in 2019 were gunna have flying cars
2019: *Meat berry*
2027: berry substitute organs
Vegans: *confused screaming*
Cannibals: *YES IT CAN BE DONE, WE CAN MAKE THE FORBIDDEN FRUIT!*
Mr. Smoosh Let’s go! I want a meat melon!
Hannibal would love this
My university is currently in the process of securing grants for comparative study of various decellurarusation techniques and their effects on various porcine tissues. However there seems to be still some inflammatory and immune responses to the reseeded ECM (not to mention still the resulting poor cell densities). I wonder whether we will be able to solve it - and how. Could we use 3d printing techniques using ECM grown from donor cells directly and seed them layer by layer? This subject is endlessly fascinating - I am a complete noob mostly approaching from transprantology perspective but this channel has prompted me to engage heavily with our bioengineering department and I am endlessly thankful for opening a whole new world to me and hopefully soon new chances for our patients. Thank You for all You do.❤
you: "no moral baggage"
my trees outside: "angry rustling"
Indeed. The idea that harming plants has no moral implications is at best, extremely arrogant...
But even the way the reasoning behind it is framed is pretty arrogant;
'It's only cruel if the thing you're harming has thoughts and/or feelings' -> 'plants have no feelings' -> 'therefore doing horrible things to plants is fine'
The first question that arises is... Is the 'plants have no thoughts/feelings' thing actually an accurate statement, and how would you even demonstrate this without making a whole bunch of anthropomorphic based assumptions...
The second question that arises is... What, precisely is so ethical about causing demonstrable harm to something but then arguing that this is OK because it couldn't feel it.
Apply that second point to a human being and it doesn't seem so reasonable anymore does it?
Is it less moral for me to slowly peel off your skin then stab you to death while you're awake, vs doing the exact same thing but making sure you're unconscious first?
That seems... Questionable, yet it's basically the same reasoning...
@@KuraIthys ima go burn down a forest after reading that
@@ExpiredCartonOfEggNogg they'll replant themselves and come after you
@@KuraIthys paper's whisper death to me in silent tree language
@@KuraIthys ok boomer
They did biology on a grape
I forgot about this meme
Biotechnology
Grape is biology itself
It would be biotechnology in this case
@@Doct0rLekter shut up, it's just a meme
I wonder if you could use an artificial scaffold, like a 3D printed forearm bone or something
Wow! That berry sure came out looking quite meaty if I do say so myself!
If high school science classes taught me anything it’s that you don’t wear flip flops in the lab
Nice profile pic
*anywhere
"Hey dude, I made a meat berry" *plap plap plap plap*
YOU WEAR THIC BOOTS
Oh yeah?! Im going to wear flip flops in the lab AND run with scissors, HOW ABOUT THAT?!
Me: Please youtube I just want something normal to watch
TH-cam: M e a t g r a p e
Dan this is TH-cam. There is nothing normal here.
*Neat*
I took a fat nap watching this video, thank you!
i’m watching this at midnight before school
Scientist: Making a meat grape*
Vegans: *confused screaming*
Me right now
Imagine the vegan teacher watching this
@@oof5281 She would say, "We don't hurt animals or their cells."
Really cool fact, most vegans actually support this process as most wish to not harm animals and this process doesn’t
@@colins4050
I doubt that's completely true because this process uses cow fetus.
2018: they did surgery on a grape
2019: they did surgery with a grape
2020: grapes becomes sentient
2021: grapes becomes human
2022: grapes becomes surgeon
2023: grapes becomes self aware
2024: grape runs for president
2025: grapes starts WWIII
2026: grapes take over the world
You just copied a comment and got 12 free likes...
A grape would still do a better job than Trump.
alterego I doubt that
Ah yes, the grapist.
LilArmalite lol