Triple Sugar Iron (TSI) Agar

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[@ms.jessicaofficial4069]

To avoid confusion, the slant uses lactose/sucrose for metabolism and the butt uses glucose for metabolism

[@munisapulatova5490]

does it say something about whether bacteria is facultatively anaerobic/aerobic??

[@yarelyguerra605]

Thank you so much for explaining so clearly, and for teaching me what my Micro professor can't!

[@ghaidaaness5405]

You’ve been helping me out lately and am so thankful for you ❤

[@carlosescudero3161]

You are definitely on my top ten most useful youtube channels. Just, Thank you so much :)

[@dogakan9857]

I can't tell how much appreciate I am. thank you so much

[@hannaholubaiye2148]

Yes, I remember using this agar for identification of my bacterial isolates from rhizosphere soil of red pepper, Capsicum frutescense.

[@cynthiameghan36]

Thank you so much! You've made it easier for me to understand 🥺

[@nyawirawaithaka4993]

Thank you for explaining this very confusing experiment. Makes perfect sense now. God bless you!

[@CatalystUniversity]

You're very welcome!

[@moemoenwe4556]

The best explaination !! Thank you

[@gennexcombatir8126]

TY! very well explained. I love you so much

[@maricarmen8776]

Thanks so much, I was so confused. So well explanatory. :)

[@CatalystUniversity]

Glad this helped you!

[@fadelmilad3732]

You aced this confusing test. Thanks a lot!

[@simonberhe6036]

You are the best, thank you!

[@moussamohammed8849]

Thanks A lot Doc 🙏

[@ateebhadi6334]

thank you sooooo much ,you're incredible

[@estiakshakil2663]

Excellent Sir

[@alyssaraechellenichols8841]

Thank you!!

[@sophiakangah3374]

God bless you for making it soo much easier for me. 🙏

[@slaughtergang3605]

You're a genius man !!

[@mauibuilt50]

Great explanation, thank you!

[@CatalystUniversity]

You're very welcome!

[@nikhatanjum3156]

U Explained so easy

[@videogamenut1289]

Thanks so much for making this video I was so confused before this lol

[@mohitthakur9919]

Thank you this helped a lot

[@CatalystUniversity]

Thank you, Mohit.

[@caroljackson767]

Thank so much. I came so confused and left a pro.

[@AT-sk7nm]

Ur such a good explainer omg…..

[@titoflash1212]

Thx!!! Excellent!!!!

[@BABENSKIE1]

Thank you so much !!!!

[@delmyurbina2043]

Thank you so much for your help, this was so clear to understand. Thanks

[@rawanhassan4166]

Thank you very much !!!!!

[@abhishekbalakrishnan1098]

Thank you really helped me to understand the concepts.....

[@CatalystUniversity]

Glad this video helped!

[@bxwn8931]

@@CatalystUniversity Hello ,
Can you answer the above question about " why that impossible to see A/K ?"

[@maryam00005]

You made it ridiculously easy

[@rbutterfly87]

Thank you!!!!

[@ghalam1246]

Thanks 🙏 ♥️

[@victoriaduke1304]

Love thisssss

[@walaabdeljawad1439]

thank you so much

[@CatalystUniversity]

No problem!

[@rafaelcalderon3681]

Give Kevin a raise!

[@user-mh9lz8et2p]

Well understood

[@user-kn9yl6dl1b]

Good work ,I don't know why it looks now super easy😂😂😂

[@fredaziku4668]

Perfect

[@Khaledf]

Thank you! I was wondering about Salmonella why it is red slope although it ferments glucose ( theoretically forming acid which means yellow color) !

[@Dragon-Slay3r]

Now left thumb from same hand has a long sword in the middle from the bird table situation they can keep the skin scales 😂

[@ewwxaca]

Thankkkssss

[@CatalystUniversity]

No problem, April.

[@rubyblackcat4310]

Thanks

[@CatalystUniversity]

No problem!

[@daphniefarkas5703]

This channel is going to save my lab grade. My instructor doesn't explain anything.

[@abdullahsalah917]

is this test is specific for gram negative bacteria only ??? I mean is it used to differentiate between gram negative species only ??? or also gram positive species ??

[@immadisarojini2775]

In short
Acid (yellow ) produced by glu metabolism is able to mask redness of peptones only partially
But
Acid (yellow ) produced by lactose metabolism is able to mask redness of peptones completely

[@immadisarojini2775]

Since peptide metabolism is always present 🎁

[@Sarah-cp5br]

Thank u so much, i don’t have time for a 20 minute long video ❤️

[@castonemwaniki5355]

You didn't talk about the specific organisms involved

[@kerzieonchong-tom5904]

hi! hopefully someone can help me! so tubes that are ALL yellow only ferment lactose? and tubes with red & yellow is safe to say that can ferment lactose AND glucose? and tube with ALL red does not ferment lactose OR glucose?

[@ajitmishra7666]

Sucrose has any role ??,Nice explanation

[@bxwn8931]

Sucrose and lactose have the same pathway.

[@lirabilgera5016]

What happened to Sucrose?

[@28roar]

so what is being fermented on the 5th tube? Why is it A/K unlike the K/A that ferments only glucose??

[@sophiakangah3374]

It is A/K and not K/A because of lactose or sucrose metabolism on the slant and a moderate glucose metabolism on the butt.

[@abdelhalimyousef1041]

You did not mention sucrose?

[@paws4life928]

What about A/k?

[@akankshapurohit6554]

❤️❤️❤️

[@ucheozo7093]

This is too simplistic

[@cpdaily703]

Thank you for your well explanation. I would like to ask you that when Bacteria only fermented Glucose(Moderate acid production), and not Lactose (tons of acid production), why it turns Yellow only in the butt and not the slant. As you know, the whole media contains the same ingredients (peptones, glucose, and lactose), so everywhere in the tube is the same. What happened to the slant, why it does not turn Yellow as at the bottom part of the tube. Is there any reactions or metabolism that stop the Slant from being Yellow as the butt?

[@ajitmishra7666]

Same doubt

[@bxwn8931]

I'm asking about the same thing 😕

[@ajitmishra7666]

@@bxwn8931 hello🙋‍♂️,you are from which country ?😁

[@bxwn8931]

@@ajitmishra7666 Hi , from Jordan 🙂

[@ajitmishra7666]

@@bxwn8931 best wishes and greetings from India🌷

[@florenceboon4818]

can i ask what actually is the gas produced?

[@priyankarchand1406]

it is either carbon dioxide or hydrogen

[@user-mh9lz8et2p]

Thank you next time include even organisms that can be I dentfied

[@nehemiahwilson6864]

Dude, love the vid! But drop the "Alk" and join the rest of us here on earth.
Other than that, AMAZING!

[@MehediHasan-gz8dp]

❤️‍🔥❤️‍🔥❤️‍🔥❤️‍🩹