Ninhydrin Test

Qualitative Test of Protein and Amino Acid
Ninhydrin Test
The ninhydrin test is a chemical test which is used to check whether a given analyte contains amines or α-amino acids. In this test, ninhydrin (a chemical compound with the formula C9H6O4; IUPAC name: 2,2-dihydroxyindane-1,3-dione) is added to a test solution of the analyte. The development of a deep blue colour indicates the presence of ammonia, primary/secondary amines, or amino acids in the analyte.
The amino group belonging to a free amino acid undergoes a chemical reaction with ninhydrin, which behaves as an oxidizing agent. When exposed to ninhydrin, the amino acid undergoes oxidative deamination, resulting in the liberation of CO2, NH3, and an aldehyde along with hydrindantin (which is a reduced form of ninhydrin).
Now, the ammonia goes on to react with another ninhydrin molecule to form diketohydrin (which is also known as Ruhemann’s complex). This complex is responsible for the deep blue colour. When the analyte contains Imino-acids like proline, a yellow coloured complex is formed. When asparagine is used, the colour of the resulting complex is brown.
The amino group of the analyte forms a compound similar to diketohydrin. This compound has a deep blue colour, often referred to as Ruhemann’s purple.

[@roshnibhatt2801]

Thankyou so much Ma'am, I learnt all the tests in very few times by your videos 👍

[@vijaypratapchoudhary763]

Very well explained

[@gurwantsingh8063]

Thank you so much Mam

[@overjoyedvids8248]

Tysmm mam it's very simple and easy way to understand ❤

[@sahilparwaj6274]

Thanks a lot 😊😊😊

[@muhdaqib9155]

Thank you❤

[@mdsaquib3057]

Thanks

[@bananirout4041]

Intense blue colour is absent

[@sahajjain3997]

thanks

[@ayeshahanif557]

The music is creeping me out tbh

[@karishmasrivastava3117]

What is the easy thing we can take in sample solution

[@4ngryNRD]

Thank you for explanation. But gloves...

[@ruttaba59]

First ma ap na test tube ma sample solution liya phir apna test tube ma 7 to 8 drop s ninhydrin solution add kiya next phir apna kaya add kiya ha test tube ma plz bata da

[@wageerawageera1861]

Informative test
Question is
Why It is just used to alpha amino acids not others? 🧐

[@mohitpanchal9313]

because in the mechanism only 1 degree amines can react so proline etc wont give this test as they are 2 degree amines

[@sameerawate586]

Anyone doing spirulina research reply me pls

[@varshaba5808]

what is the sample solution

[@nareshsabavath3066]

Sample solution means any sample containing protein for eg:albumin etc.