Xanthoproteic Test

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  • เผยแพร่เมื่อ 21 ส.ค. 2024
  • Qualitative Test of Protein / Amino Acids
    Xanthoproteic Test
    Xanthoproteic test is used to detect amino acids containing an aromatic nucleus (tyrosine, tryptophan and phenylalanine) in a protein solution which gives yellow color nitro derivatives on heating with conc. HNO3. The aromatic benzene ring undergoes nitration to give yellow colored product. Phenylalanine gives negative or weakly positive reaction though this amino acid contains aromatic nucleus because it is difficult to nitrate under normal condition. On adding alkali to these nitro derivative salts, the color change from yellow to orange.

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