Ninhydrin test: test for alpha amino acid in HINDI

The ninhydrin test is a chemical test which is used to check whether a given analyte contains amines or α-amino acids. In this test, ninhydrin (a chemical compound with the formula C9H6O4; IUPAC name: 2,2-dihydroxyindane-1,3-dione) is added to a test solution of the analyte. The development of a deep blue colour indicates the presence of ammonia, primary/secondary amines, or amino acids in the analyte. The amino group belonging to a free amino acid undergoes a chemical reaction with ninhydrin, which behaves as an oxidizing agent. When exposed to ninhydrin, the amino acid undergoes oxidative deamination, resulting in the liberation of CO2, NH3, and an aldehyde along with hydrindantin (which is a reduced form of ninhydrin).
Now, the ammonia goes on to react with another ninhydrin molecule to form diketohydrin (which is also known as Ruhemann’s complex). This complex is responsible for the deep blue colour. When the analyte contains Imino-acids like proline, a yellow coloured complex is formed. When asparagine is used, the colour of the resulting complex is brown.
#ninhydrine #alphaaminoacid #pkprabhakar

[@rr.studios]

Thank You for including the phrase "in HINDI" so non-Hindi speakers would know.
I appreciate it!

[@clinicalbiochemistry]

So nice of you

[@nonstop-1259]

This video is so helpful sir... Tomorrow is my exam and I had lots of doubts on this topic, your video cleared it very well...Thanks a lot😇😇

[@clinicalbiochemistry]

Good luck for your exam...

[@akshayjouhary3897]

Thanks sir,good job

[@clinicalbiochemistry]

Most welcome...Thanks a lot...keep watching...

[@raqiahumar9130]

Awesome 🤩

[@Progamer-ui4tp]

U should join pw sir

[@technicalanuj4226]

Nice explanation sir 😊😊

[@laxmansutar1294]

Sir thanks for video 🙏

[@clinicalbiochemistry]

So nice of you..keep watching

[@GaneshGuptaAssam]

Mujhe gamma Aminobutaric Acid content tea me test karwana hai. Kya aap help kar sakte hain.

[@mahimahikhan1388]

Thanks sir

[@clinicalbiochemistry]

So nice of you

[@dahiyaeducation2397]

Sir thanku

[@akshayjouhary3897]

Thanks sir,good job

[@clinicalbiochemistry]

Most welcome...Thanks a lot...keep watching...