Lead acetate Test

Qualitative Analysis of Protein / Amino Acids
Lead acetate Test
Lead acetate test (or Lead sulfide test) is a biochemical test for the detection of amino acids like cysteine and cystine. The test is a specific test for the detection of amino acids containing sulfur, S-S group in cysteine, and S-H group in cystine. The sulfur-containing amino acid such as cysteine, cysteine, and methionine (sulfhydryl/thiol group) reacts with lead acetate under alkaline conditions to form a brown precipitate. These sulfur-containing amino acids are degraded in strongly alkaline media to release sulfide ion (S2-) in the form of H2S (hydrogen sulfide). The sulfide ions can react with lead (II) acetate to form a brownish-black precipitate.

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