Bioprocessing Part 3: Purification

แชร์
ฝัง
  • เผยแพร่เมื่อ 29 ต.ค. 2013
  • This video is the third in a series of three videos depicting the major stages of industrial-scale fermentation: fermentation, separation/recovery, and purification. This program describes the process by which a biological product-in this case, a pure, concentrated solution of Green Fluorescent Protein (GFP)-is derived from clarified lysate. This is accomplished through multiple variations of two basic procedures: chromatography and filtration.

ความคิดเห็น • 49

  • @josebarcelona11
    @josebarcelona11 4 ปีที่แล้ว +43

    This series was amazing. I currently work in a biomanufacturing facility and these videos are pretty spot-on. They closely resemble what we do, only this one is a smaller scale and a different bacterium/product. Amazing job.

    • @Superlady1234
      @Superlady1234 2 ปีที่แล้ว +1

      Where. Are you working,

  • @leoniewong2842
    @leoniewong2842 10 ปีที่แล้ว +37

    Very valuable set of videos. I learned about most of the unit operations in class, but for a long time I had difficulty finding sources that gives a detailed run down on typical steps involved in producing biologically derived products in sterile manufacturing environment. I was surprised how much information was conveyed in these videos. Thank you so much, the videos definitely consolidated my knowledge on fermentation processes.

    • @realstehekin9521
      @realstehekin9521 7 ปีที่แล้ว

      these aren't sterile steps, they are clean. Several steps are taken after purification to sterilize and package the product. I'm in the biz of purification.

  • @Aj-lw1ej
    @Aj-lw1ej 2 ปีที่แล้ว +2

    the series is one of the best. i have ever seen .please continue

  • @sharmilap4697
    @sharmilap4697 ปีที่แล้ว

    Excellent video series for someone that wants to learn the bioprocess and its steps. Thanks so much!

  • @ithirstyforknowledge
    @ithirstyforknowledge 4 ปีที่แล้ว

    Cant thank enough for this well explained detailed yet concise video

  • @daamu48
    @daamu48 7 ปีที่แล้ว

    AN EXCELLENT VIDEO. EXCELLENT NARRATION, EASY TO UNDERSTAND.HIGHLY RESOURCEFUL FOR P.G STUDENTS.

  • @lesliehilesgardener6959
    @lesliehilesgardener6959 ปีที่แล้ว

    The CREATOR IS MY GOAL. IM ALMOST BACK WHERE I BELONG

  • @longbelao
    @longbelao 4 ปีที่แล้ว +1

    Real education! Love it!

  • @sreevidyatanjavuru7049
    @sreevidyatanjavuru7049 8 ปีที่แล้ว

    it is wonderful to know about fermentation products,thank you

  • @frominside8223
    @frominside8223 2 ปีที่แล้ว

    Thank you very much for all three contents.

  • @amnindersekhon1817
    @amnindersekhon1817 ปีที่แล้ว

    Thank you this series, this was extremely helpful.

  • @mobr.
    @mobr. 6 ปีที่แล้ว

    Thank you. This video was very informative.

  • @darpanapatel6081
    @darpanapatel6081 3 ปีที่แล้ว +4

    I watched all three videos and they were super helpful in understanding the process from start to finish. Thank you and great job!

  • @InspiringPaises
    @InspiringPaises 2 ปีที่แล้ว

    Very good explanations!!

  • @h.o.d.-b.t.3584
    @h.o.d.-b.t.3584 4 ปีที่แล้ว +1

    this is very useful video. entire set is so... thank you. it covers all aspects beautifully well.

    • @amanihayat1635
      @amanihayat1635 ปีที่แล้ว

      Summary of steps for three videos ?

  • @user-jq2ui6dy6q
    @user-jq2ui6dy6q ปีที่แล้ว

    Thanks a lof for the clear explanation about purification steps. It helps a lot :)

  • @florianlightspring2274
    @florianlightspring2274 8 ปีที่แล้ว +2

    Really amazing what Kind of technological steps are ready.

  • @user-si8cb5hq1c
    @user-si8cb5hq1c 8 ปีที่แล้ว

    Thanks for this formation

  • @thatsmellsdelicious5146
    @thatsmellsdelicious5146 2 ปีที่แล้ว

    Wonderful videos! Using this to prepare for interviews :)

  • @seramimi7
    @seramimi7 ปีที่แล้ว

    Super awesome videos, thank you.

  • @meissnerfiltrationatendime5580
    @meissnerfiltrationatendime5580 7 ปีที่แล้ว

    very interesting video !!

  • @misa143me4
    @misa143me4 3 ปีที่แล้ว +1

    Thank u, the video helps me to understand a lot of papers in one moment :)

    • @xen2807
      @xen2807 3 ปีที่แล้ว

      hi misa, im looking forward to do a PhD, can you guide, please

  • @huwaidakhairulanwar3534
    @huwaidakhairulanwar3534 6 ปีที่แล้ว

    Thank you for the good video🎈🥇🥇🥇

  • @sandhyapalakkagari1469
    @sandhyapalakkagari1469 2 ปีที่แล้ว

    Only 3 videos are there i understand these videos very well and expecting more videos about fermentation .

  • @parthibanezhumalai2949
    @parthibanezhumalai2949 9 ปีที่แล้ว +1

    its very gud to know

  • @jabulanimasukume8820
    @jabulanimasukume8820 5 ปีที่แล้ว

    best video

  • @atharvakulkarni8818
    @atharvakulkarni8818 11 หลายเดือนก่อน

    Amazingg videos...!!😊😊...make part-4 as well...about freezing and drying.🙂

  • @sivakumarreddy8687
    @sivakumarreddy8687 3 ปีที่แล้ว

    Looking for complete industrial scale protein purification setup. Can anyone suggest who can make customized instrument makers and services?

  • @ExitStatement
    @ExitStatement 6 ปีที่แล้ว +4

    FINALLY IT'S GREEN!

  • @fatimaalubidi6194
    @fatimaalubidi6194 3 ปีที่แล้ว

    Wonderful..... from iraq

  • @ehabfarouk6976
    @ehabfarouk6976 ปีที่แล้ว

    Great

  • @shaikfasiya
    @shaikfasiya 3 หลายเดือนก่อน

    No words to say. It's really amazing & informative.

  • @PEMDASbaby
    @PEMDASbaby 10 หลายเดือนก่อน

    WOW

  • @thatsmellsdelicious5146
    @thatsmellsdelicious5146 2 ปีที่แล้ว

    TAOI
    Time
    activities
    operation
    Instrument

  • @Here0s0Johnny
    @Here0s0Johnny 7 ปีที่แล้ว +2

    who needs purified GFP on this scale?

    • @rhythmandacoustics
      @rhythmandacoustics 6 ปีที่แล้ว +4

      probably will be separated into smaller bottles and sold to other private analytical labs and universities.

    • @Quincy8Boy
      @Quincy8Boy 6 ปีที่แล้ว +2

      It's just for the class. It goes down the drain.

    • @kimlassiter8904
      @kimlassiter8904 5 ปีที่แล้ว

      It’s used in genetic coding

  • @weimullerjohann9118
    @weimullerjohann9118 2 ปีที่แล้ว

    Ph Sensor has every child . Take ninhydrin and not so nonsense !

  • @emilianomendezdelarosa9908
    @emilianomendezdelarosa9908 ปีที่แล้ว

    chad

  • @nazirahmed4809
    @nazirahmed4809 2 ปีที่แล้ว