According to the manual of Superdex prep grade, the packing should be done in two steps for this resin. Step 1 according to the table at 5:33. After removing the reservoir, Step 2 at a higher flow rate.
Our scientific support team will be able to help answer your question. You can contact them here www.cytiva.com/support/contact-us I hope that helps 🙂 Best wishes, Sophie / Cytiva
please if you can explain to me, why the mobile phases containing a concentration of ammonium sulfate promote the binding of many proteins to HIC columns??
High salt reduces penalty to strip waters that cage hydrophobic patches on the protein surface. Hence, easier to minimize solvent-accessible surface and stick to the resin.
According to the manual of Superdex prep grade, the packing should be done in two steps for this resin. Step 1 according to the table at 5:33. After removing the reservoir, Step 2 at a higher flow rate.
how about packing parameter for Sepharose CL4B ??
may i know what is the filter pore size in the reuseable funnel ? thank you
Our scientific support team will be able to help answer your question. You can contact them here www.cytiva.com/support/contact-us
I hope that helps 🙂
Best wishes,
Sophie / Cytiva
please if you can explain to me, why the mobile phases containing a concentration of ammonium sulfate promote the binding of many proteins to HIC columns??
High salt reduces penalty to strip waters that cage hydrophobic patches on the protein surface. Hence, easier to minimize solvent-accessible surface and stick to the resin.
What is packing solutions
That should be in the manual for the resin. Should be a weak buffer system, but check with the resin manual.
how did you do it can you share with me , thank you
you're packing an xk 16/70 and yet there are no instructions to that column on the packing parameters table