How to pack an XK chromatography column

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  • เผยแพร่เมื่อ 24 ธ.ค. 2024

ความคิดเห็น • 10

  • @KBuessow
    @KBuessow 2 ปีที่แล้ว

    According to the manual of Superdex prep grade, the packing should be done in two steps for this resin. Step 1 according to the table at 5:33. After removing the reservoir, Step 2 at a higher flow rate.

  • @arisugihsubagja1460
    @arisugihsubagja1460 11 หลายเดือนก่อน

    how about packing parameter for Sepharose CL4B ??

  • @ykit212
    @ykit212 2 ปีที่แล้ว

    may i know what is the filter pore size in the reuseable funnel ? thank you

    • @Cytiva
      @Cytiva  2 ปีที่แล้ว

      Our scientific support team will be able to help answer your question. You can contact them here www.cytiva.com/support/contact-us
      I hope that helps 🙂
      Best wishes,
      Sophie / Cytiva

  • @sh7arwt3teer99
    @sh7arwt3teer99 3 ปีที่แล้ว

    please if you can explain to me, why the mobile phases containing a concentration of ammonium sulfate promote the binding of many proteins to HIC columns??

    • @vladimirtalibov980
      @vladimirtalibov980 2 ปีที่แล้ว

      High salt reduces penalty to strip waters that cage hydrophobic patches on the protein surface. Hence, easier to minimize solvent-accessible surface and stick to the resin.

  • @IloveLeeDaHye
    @IloveLeeDaHye ปีที่แล้ว

    What is packing solutions

    • @cementheed
      @cementheed ปีที่แล้ว

      That should be in the manual for the resin. Should be a weak buffer system, but check with the resin manual.

  • @kangeric9102
    @kangeric9102 3 ปีที่แล้ว

    how did you do it can you share with me , thank you

  • @barbaramarcolin1527
    @barbaramarcolin1527 11 หลายเดือนก่อน

    you're packing an xk 16/70 and yet there are no instructions to that column on the packing parameters table