Protein Purification
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- เผยแพร่เมื่อ 13 มิ.ย. 2024
- Protein Purification aims to isolate a single type of protein from a biological tissue or culture. This video explains the most common methods applied to purify proteins, which are:
Precipitation methods, filtration methods, electrophoresis and chromatographic methods.
It is important to have an overview of these techniques in order to choose which method/methods suit the project best. Factors that can influence such a decision are: the desired level of purity, the accessibility of required equipment and the characteristics of the target protein. In practice multiple purification techniques are often combined.
Time Code:
00:00 - Intro
00:20 - Content
00:49 - What is protein purification?
02:20 - Separate your protein from a biological tissue
02:53 - Choosing the right purification techniques
03:40 - Precipitation methods
03:58 - Salting out
05:10 - Chromatography
06:08 - Affinity chromatography
07:25 - Ion Exchange Chromatography
08:07 - Hydrophobic Interaction Chromatography
09:08 - Size Exclusion Chromatography
10:20 - Filtration Methods (Dialysis)
11:12 - Electrophoresis
12:08 - Native PAGE
13:29 - Outro
At Cube Biotech we are experts in the purification of protein with over 40 years of experience. We offer a wide range of protein purification products:
cube-biotech.com/products/pro...
Furthermore, we offer custom protein purification:
cube-biotech.com/protein-serv...
For general inquiries about our company check out:
cube-biotech.com/
For more detailed information about protein purification techniques check out:
Liu, S., Li, Z., Yu, B., Wang, S., Shen, Y., & Cong, H. (2020). Recent advances on protein separation and purification methods. Advances in colloid and interface science, 284, 102254. doi.org/10.1016/j.cis.2020.10... - วิทยาศาสตร์และเทคโนโลยี
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The video was really useful. One of the bests I’ve watched ever on TH-cam 🥂💯
Thank you for the compliment! Great to hear that it was worth the effort :)
good video, it breaks the concept down to a layman's understanding. you made education interesting.
This is a master piece, believe me after a month in lab this is so accurate
Thank you very much! We appreciate that the effort that went into the video is recognized!
@@CubeBiotech well done , you deserve it .
I would be very happy if you do one for (Quantification of protein by Mass spectrometry) .
Such a good overview. Love the effort 🤩
Thank you! That was our intention.
It was REALLY useful, thank you so much!
Thank you! Happy that our work for that video payed off :)
Well simplified. Thank you for the good work.
Hi Patrick, thank you for the positive feedback! That is always appreciated :)
This is the most useful video I ve seen on this topic so far
Thank you very much. We are really glad to hear that!
The video was of great help to me. Thank you
Thank you! Glad that we could help!
Wowww I am beyond amazed. This lesson was explained to us in 2 hours and was so tiring and confusing but you managed to clear it all out in 13 minutes. So grateful 🙏🙏 definitely recommending this channel to my classmates❤
Thank you very much for these kind words! That definetly made the day of the lead creator of this video! Thank you for further recommending us!
Beautiful presentation :') thanks
Glad that you liked it!
This video is amazing and really helpful! Thanks :)
Really Glad to hear that. It makes us happy that the effort was worth it!
@@CubeBiotech 💜
amazing! good job! 🤩
We are glad that you like it :)
Very Good and useful.thanks for describing so deeply
Glad that we could help :)
Many many thanks for this explanation ❤
We are happy it helped you. What part of the video did you find the most helpful?
This was really helpful 🙂
Thank you! Glad that you liked it.
it is a brilliant video
Thank you :) We are glad that you liked it!
Thanks a lot ❤
You are welcome :)
03:38 precipitation method, salting out
In native page , sds is not used , and proteinsc are. Not separated by charge
Thus all don't have Negative charge
Hello from California! I have a question. Do Golgi in all types of cells (liver and kidney cells) have the same function and structure ? If we were to purify the Golgi ( fractionation) from different cells, (not plant) would they function the same and have the same structure ??? I can’t seem to grasp this concept.
Good Day :)
We are experts on protein purification and not the Golgi-Apperatus. We do not feel comfortable to answer this for you.
What we do recommend to ask this question on platforms like Researchgate. You should find your answer there.
Thank you so much for your guidance!!!
Great teaching ,I enjoyed it
But please ,I couldn't get a particular word you were saying at 8:34,,,, did you say soluble???
Please I'll appreciate a response,, thanks in anticipation
Yes that is what was said.
"The protein is just soluble."
@@CubeBiotech thanks a bunch
@@elizabethmonday3751 You are welcome :)
❤️❤️❤️
We are glad that you like it!
SDS PAGE plz
❤❤😍😍😍
Glad that you like it :)
can you send me the sides
There are no slides, sadly. These are, for the most part, Adobe After Effects files. :(