DNA Microarray Methodology

This animation demonstrates how DNA microarray experiments are performed. One common use of microarrays is to determine which genes are activated and which are repressed when two populations of cells are compared. Every gene is measured simultaneously. As an example, we’ll compare what happens to yeast genes when cells are grown in aerobic versus anaerobic conditions.
Written by:
A. Malcolm Campbell, Ph.D.
Copyright © 2017 NC Community Colleges and BioNetwork

[@GrandeNutty]

This is beautiful.
I swear, my prof made this sound like the most complicated thing in the world.

[@acalp]

Best explanation of microarray on TH-cam

[@NCBioNetwork]

Thanks for the feedback!

[@EpicSelenium34]

Excellent explanation and pristine graphics. This video really helped me comprehend this difficult topic. Thank you!

[@joshfernandez6089]

Shut ur mouth

[@bean_odinson]

@@joshfernandez6089 you didn't like the video?

[@jujubajtn]

Thank you for this video!! You helped a undergraduated student of Biotechnology here.

[@andrewscampfire]

This video is top-notch! What a great explainer video! It really helped me understand the nitty-gritty of DNA microarrays. Thank you so much!

[@haifaaferas8311]

You're a lifesaver. I needed this for my A level exam and i really didn't get it until i came across this vid

[@taladiv3415]

Thank you for saving me precious time and mitigating the frustration involved in boresome reading of an unfamiliar topic for the first time.
Now I am primed to understand the dry text.

[@englishiseasy6092]

I have seen to many videos about microarray but I can say surely this is the best. Brief and useful. Thanks 👍👍👍

[@YasmineNazmy]

This is the best scientific explanation video I have ver come across. THANK YOU!

[@khizirabbas9226]

A very useful and easily understandable animation to know about the methodology of Microarray. Thanks a lot and stay blessed. Regards.

[@coolkitty154]

Very clear and informative, thank you!

[@ananyapaul9148]

this animation was too good. best way to explain 👏👏👏👏👏👏

[@qp5337]

Really great explanation, the visuals really helped. Thanks

[@makiiu]

best explanation to understand microarrays, thanks

[@rogsang9764]

excellent video, the graphics are trully helpful, thanks for share this valuable information

[@silentcloud6342]

this video is the best microarray explaination.

[@msmemoona7198]

Excellent presentation

[@Kh471D]

best. medical. animation. iv'e. ever .seen.

[@Forensicforumexpert]

very nice explanation!

[@joaogoncalves5480]

Remarkable work and explanation!

[@ankitachowdhury5268]

Very nice explanation mam...plz upload more videos like these...it's really helpful...

[@dinaaly2082]

great video

[@ruhidyusifov5106]

Thank you so much

[@NCBioNetwork]

You're most welcome

[@kerolloskamelsaeed5615]

Wow that was helpful and also entertaining.. thank you so much

[@dinatjahanmilitasnim4614]

Thanks a lot

[@sophianotopoulou262]

the best explanation!!!

[@tarunkadian1178]

Thanks a lot ...superb explanation😊

[@TheNaiaratarifa]

Hi folks! excellent animation, thanks! But can you enable the CC subtitle option that allows others to create subtitles? I really want to translate it to Portuguese. Thanks!

[@NCBioNetwork]

Added! Sorry for the delay.

[@mulhmzuhili]

Yep this is best one.

[@faisalbinrahman9722]

it was very informative. thank you very much. :)

[@afreenfatima1004]

really good video.. but i have a question..
when we add cDNA (probe) on DNA chip having 6000 genes of yeast then how this probe will bind to that DNA present in gene sequence?
bcz DNA is a double stranded molecule

[@rasheedolajiire5135]

The single stranded DNA copies are made from the genes and can easily hybridize with the probes (which are also single stranded DNA)

[@mylordandsaviour4786]

1:02 Did they change the tip?

[@marsolata]

of course they should do that

[@pratyush136]

excellent

[@paulahrens6362]

could you do videos on other analysis like w-blot or ELISA ? Anyone here with good recommendations?

[@gauthampaul4213]

what apparatus is used to wash off the unbound cDNA?

[@mohammadshormij315]

very good thank you

[@itsrooh645]

loved it

[@noorulain6066]

Amazing , excellent explanation ❤👏👏❤

[@devanshuradia3466]

thank u so much.. great explanation

[@Nooneonearth2.0]

is it possible to model the DNA microarray in microfluidics?

[@zeelpatel8049]

thank you

[@DanielTerceiro9]

very good!! thank you very much!

[@NCBioNetwork]

Thanks for the feedback. We are glad the animation is helpful to you.

[@hamzashaikh3122]

which software you use for animation

[@ajibara9970]

AMAZING

[@brendasolangeayzanoacanale7831]

awesome!

[@mihaleo3216]

Excelent grafics

[@AhmedHamed-ot1ez]

Perfect
Thanks

[@王浩源-v3q]

1:35 but these are red and yellow lol

[@Le_Marquis_de_Faux_Images]

SCIENCE!!

[@shree9728]

How's the mRNA is converted into red or green cDNA?

[@frangimenez4674]

A reverse transcriptase is applied to the mRNA, which makes the cDNA. Then, this cDNA is marked usually with a fluorescent compund. That fluorescence is what the scanner analyzes and allows to differentiate the different cDNA strands

[@shree9728]

@@frangimenez4674 thank you Sir...

[@akhil.nitrkl.22]

Wow🔥

[@shabinabano275]

👌👌👌

[@hanchung8237]

For my final.....

[@JYOtiRaNJanMANgaRaj]

🙏🙏👌👌👌👌

[@student_remo]

❤

[@Shakeel-]

👌

[@somerandomperson3970]

Yeah, Gen-Z will be a bunch of geniuses

[@056vatsalapandey3]

PPEERRFFEECCTTTTTTTT

[@BeeRich33]

Still seems rather manually intensive. Ideally automated much like bloody chemistry analysis.