This is the best video for explaining the concept of Salting in and Salting out.... Thankyou dada for this....l would like to ask you about the reference where l can get the exact text of this explanation!!!!!
I usually have a hard time understanding how some of these processes work but you did an amazing job at explaining the process! I feel like I understand it and could explain how it works to another individual now. Thank you!
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
Great work, thank you for your presentation, it was clear and informative. the use of graphics greatly helps, keep it up! Would love more about protein separation and electric properties.
Ammonium sulphate precipitation follows the procedure explained by you. It is the good and simple method instead of ultracentrifugation and other methods. Nicely explained. Continue.
Could you please help me by sharing my contents with your students . I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@@animatedbiologywitharpan sir please make a video on water and their bond forming mechanism with biomolecules . Please sir this mine humble request to you.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@animatedbiologywitharpan I am really interested in your video! Could you let me know more about the detailed mechanism of the Salting-in step? From your video, I understand that adding salt ions can make the structure of the protein stable along with reducing of the intermolecular replusion of the protein. Then, what is the mechanism of the water molecular can form the hydrate shell around by the protein? Is hydrogen bonding on the hydrophilic regions of the protein? Thank you!!!!
I understand salting in and salting out based on their definitions, but I don't understand why at 1:33 the definitions are placed on either side of the graph like they are? I would assume salting in would be in the left side, and salting out on the right? Edit: There is a clearer graph at 4:53 with the definitions on the "correct" side.
Tysm, this was really useful and informative for me. I’ve just had a lecture about protein separation at the university and got confused but your animation helped me so much
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Great video! Just have a litte remark: At 1:52 you mark the carboxylate ion which is part of the protein peptide backbone. Instead you should have marked the carboxylate at the amino acid side chain which is exposed to the enviroment.
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
Thanks ! I was looking for salting out mechanism in saponification. I hope the mechanism will be similar, as both protein and soap are polar. Thank you !!
Great bro. Could you talk about why Lanthanum.Cl3.H20 (anhydrase) precipitate in room temperature water? (it has low solubility for sure). This question has nothing to do with protein solubility.
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Small contribution like $5 means a lot for us.
please if you can explain to me, why the mobile phases containing a concentration of ammonium sulfate promote the binding of many proteins to HIC columns??
Beautifully explained thank you! I've been struggling with sample preparation for months now - I have VERY expensive triple-labelled samples for NMR so, naturally, sample stability has been a great concern to ensure we can get the most out of each prep. Have you got any rough kind of buffer optimisation "workflow" that you go through when determining protein solubility? I've thus far tried crude hanging drop precipitate screens using a crystallographers microscope, as well as a thermal denaturation assay utilising a hydrophobic residue-binding dye which infers protein unfolding using qPCR (ThermoFluor I believe is the technique name). Have you got any other suggestions? I'm still struggling somewhat with long term stability.
sir can you please tell me why and how high molecular weight has less surface area and that leads to less hydration less salt required for precipitaion of protein in case of globulin which is know as half saturation and which is exact opposite for albumin that ppt in full saturation
however i have a question, regarding the protein isolation, if i not sure about the solubility strength of my desire protein, then salting in (addding low concentration of salt solution to allow it to solube is better) or salting out is better. And base on explanation, i understand that both salting in and out is helping to isolate, is there any condition like protein that not likely to soluble will be favor for salting out process? if there is, is the example fibrous protein will be more easier to seperate in salting out or salting process? Thank you for answering, look foward to knowing more on this
I think with high molucal weight..we in are in case water in molecule. When the molecule is very low weigheit we are in case in molecule in water.. I think in hight salt the high molecule began to contract and push water in outside. We can see that in milk coagulation by elevating temperature.. I think all high molecular product can forme an emulsion Water in oil or oil in water.. Thank you for video..👍👍🇩🇿🇩🇿
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@@animatedbiologywitharpan I just wanted to take a moment to appreciate your incredible work. Your unique teaching style is truly refreshing and inspiring. You have a remarkable ability to present complex topics in such an engaging and accessible way. Your creativity and passion for educating others shine through in every video. Thank you for making learning enjoyable and for sharing your expertise with us.
This is the best video for explaining the concept of Salting in and Salting out.... Thankyou dada for this....l would like to ask you about the reference where l can get the exact text of this explanation!!!!!
There is no 1 proper source for it ....several review articles and text books . But you can follow voet and voet
Please share my channel link with your friends and help me to reach big audience
@@animatedbiologywitharpan Ok dada....l will try to look into them
@@animatedbiologywitharpan Sure
Is there any video of Parkinson's disease made by you?
our teacher's explaining his subject with ur video, you can be proud bro
Marta Romero Marcos please share my channel link with your friends and help me to reach big audience
@@animatedbiologywitharpan She shared it with me!!! I've learnt a lot!
Thank you. You did something my textbook and my lecturer couldn't do.
no is because is very boring why do I have to study stupid biochemistry for my degre what a joke bro lol
I usually have a hard time understanding how some of these processes work but you did an amazing job at explaining the process! I feel like I understand it and could explain how it works to another individual now. Thank you!
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
You are a ray of hope for all of us aspirants like me 🇮🇳🇮🇳
Such a great refresher a year after my biochemistry courses! The graphics are really helpful, great work!
Please share my channel link with your friends and help me to reach big audience
This video really made this topic make a lot of sense when my textbook left me confused! Thank you!
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
I was struggling with this concept but you sir explained it very well. Thank you 🙏🏻
Please share my channel link with your friends and help me to reach big audience
BLESS U BRO ,SERIOUSLY 🥺😭u saved my ass in 12 hours i have my exam and this made me finally understand
💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
Share my channel link with your friends and help me as well
You are legit making biochemistry easy for me 🙌
Please share my channel link with your friends and help me to reach big audience
Best video ever and very easy to understand! i literally understood everything in 6 mins thank you so much! you are an amazing teacher!!
Please share my channel link with your friends
Great work, thank you for your presentation, it was clear and informative. the use of graphics greatly helps, keep it up! Would love more about protein separation and electric properties.
Please share among your friends and help me to reach big audience
Thank you for this lecture video. I have never understood this topic before and the mechanism behind it. This video has done it for me.
Do explore other videos I. My channel.... if you like my content please share among your friends
Our group just used your video and it was very informative about explaining the topic. Thank you!
Thanks a lot, please support our channel by super thanks. Your 1$ contribution is my motivation.
finally it's crystal clear to me...thank you so much!
please share among your friends
You saved my day within 6 mins🤗 Thank you!
Utilize the rest of the day by doing something creative 🙌
Ammonium sulphate precipitation follows the procedure explained by you. It is the good and simple method instead of ultracentrifugation and other methods. Nicely explained. Continue.
Could you please help me by sharing my contents with your students . I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Please requesting you send the procedure to me
Short and clear, thank you!
Awesome explanation. You did a finer job at explaining this concept than my professor. Thanks again.
Please share with friends. Please subscribe to my second channel. youtube.com/@letscrackbioexams94?si=ZWdPXv2NSpZeDHZu
Thank you!!! This video was very well explained and helped me understand something even my professor could not help with
Please share my channel link with your friends and help me to reach big audience
Thanks you so much dear , I have finally clear my concept with this video . Thanks to you once again .
Please share my channel link with your friends and help me to reach big audience
@@animatedbiologywitharpan sir please make a video on water and their bond forming mechanism with biomolecules . Please sir this mine humble request to you.
Best explanation for salting in and out. I want to cite this reference
Haha. Thank you so much
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@animatedbiologywitharpan
I am really interested in your video!
Could you let me know more about the detailed mechanism of the Salting-in step? From your video, I understand that adding salt ions can make the structure of the protein stable along with reducing of the intermolecular replusion of the protein. Then, what is the mechanism of the water molecular can form the hydrate shell around by the protein?
Is hydrogen bonding on the hydrophilic regions of the protein?
Thank you!!!!
I love how you teach it. Even a dummy like me can go through medical school just by watching your tutorials.
please share my channel link with your friends and help me to reach big audience
1 hour of lecture in 6 minutes... thanks!! :)
Please share my channel link with your friends and help me to reach big audience
Thank you for this. Was able to understand it better thanks to this video.
Please share my channel link with your friends and help me to reach big audiance namaste 🙏
You make it easier than I thought, thanks a lot dude.
Please share among your friends and help me to reach big audience
@@animatedbiologywitharpan one of my friends share it with us in the group 😭❤️
Ahmad Abdullrahman check out all of my playlists....might be useful
I understand salting in and salting out based on their definitions, but I don't understand why at 1:33 the definitions are placed on either side of the graph like they are? I would assume salting in would be in the left side, and salting out on the right?
Edit:
There is a clearer graph at 4:53 with the definitions on the "correct" side.
FINALLY, I understood this. Thanks so much!
Please share my channel link with your friends and help me to reach big audience
Tysm, this was really useful and informative for me. I’ve just had a lecture about protein separation at the university and got confused but your animation helped me so much
Please share my channel link with your friends and help me to reach big audience
Less time with clear concept great 😊 thanks for this 👍
Please share my channel link with your friends and help me to reach big audience
Thank you so much for the explanation 🙏. Your wise way to explain alongside the illustration helped me in clarifying some points about the topic.
Please share my channel link with your friends and help me reach out to big audience
Very informative. Quick and easy to understand. Animation make it more clear.
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Great video! Just have a litte remark:
At 1:52 you mark the carboxylate ion which is part of the protein peptide backbone. Instead you should have marked the carboxylate at the amino acid side chain which is exposed to the enviroment.
Thanks for your suggestion 😊
Explained in easy way, with drawings - the best videos 😍 thank you for your work
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
Thank you for this video it was explained very well. Got in on the first take!
Glad to know that it was helpful. Please share my channel link with your friends and help me to reach big audience
Thank you ! The concept is clear for me now😊
Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.
Thank you - very clear and concise explanation!!
Please share my channel link with friends
Helps a lot! Thanks!
Please share among your friends and help me to reach big audience
Thank you for explaining this concept clearly!!!
Please share my channel link with your friends and help me to reach big audience
Thank you! This helped a lot! I really appreciate your videos.
Please share my channel link with your friends and help me to reach big audience
I can understood so easily, Very good explanation sir
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Really useful video. Such a tricky concept made like easiest thank you 👌🏻
Please share my channel link with your friends and help me to reach big audiance
what a legend. explained better than my uni
Share in your college group
Literally bro.. Yor presentation is too good!! Thanku 👌👌👌
Please share my channel link with your friends and help me to reach big audience
Thank you so much for making this fantastic video ❤
Please support me by subscribing to mu second channel youtube.com/@letscrackbioexams94?si=l2Di6Uc4hCYYa3ve
Clear and amazing explanation. Keep up the good work!!
Please share my channel link with your friends and help me to reach big audience
I like your animations it helps give a clearer pictuce
This video was very helpful! Thank you! Keep up the excellent work!
Please share my channel link with your friends and help me to reach big audience
Thanks alot for the video my concepts are Cleared very well.
Please share my channel link with your friends and help me to reach big audience
This makes so much sense! Thank you.
Please share my channel link with your friends and help me to reach big audience
it was very informative and very clear. Thank you so much
Please share my channel link with your friends and help me to reach big audience
Thank you so much! Your explanation was very clear!
Please share among friends and help me to reach big audience
A really nice explanation. A hug from Brasil!
Love from India. Please share my channel link with your friends
Very good explanation.Thank you very much!!!!!
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
Jazak Allah khyra sir
Thanks! It was clear and easy to follow.
Please share my channel link with your friends and help me to reach big audience
Thanks ! I was looking for salting out mechanism in saponification. I hope the mechanism will be similar, as both protein and soap are polar. Thank you !!
Please share my channel link with your friends and help me to reach big audience
it was an awesome video. you explained so well. thank you soooooo much
Please share my channel link with your friends and help me to reach big audience.
Great bro. Could you talk about why Lanthanum.Cl3.H20 (anhydrase) precipitate in room temperature water? (it has low solubility for sure). This question has nothing to do with protein solubility.
Concept clearly explained❤
Please share my channel link with your friends
Thank you YOU'RE THE BEST👍
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I lovedit!!!!!! Thank you for all the informations. You saved my cryobiology exam with 6 mn. of video :))
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
Thank you for your such nice and clear cartoon!
Please share my channel link with your friends and help me to reach big audience
Thank you,You understood me very well،Keep making more videos🌹🤍
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Small contribution like $5 means a lot for us.
@ 5:17 what are the blue circles surrounding the protein? Is it water molecule?
please if you can explain to me, why the mobile phases containing a concentration of ammonium sulfate promote the binding of many proteins to HIC columns??
Wow that was a great explanation. it was very easy to understand. THANK YOU!
Please share among friends and help me to reach big audience
Also subscribe to my other channel th-cam.com/channels/4IpyopsGWSjaPACNTZLuqg.html
The video is really informative. 👍
best explanation ever!
th-cam.com/channels/4IpyopsGWSjaPACNTZLuqg.html please subscribe to my second channel as well
Great work buddy keep this up
thanks a lot
Great video short and very clear!!!!!
Please share among friends and help me to reach big audience
Protein in pI hasn't net charge. So how "counter-ions" are around the protein in that case?
Beautifully explained thank you! I've been struggling with sample preparation for months now - I have VERY expensive triple-labelled samples for NMR so, naturally, sample stability has been a great concern to ensure we can get the most out of each prep.
Have you got any rough kind of buffer optimisation "workflow" that you go through when determining protein solubility? I've thus far tried crude hanging drop precipitate screens using a crystallographers microscope, as well as a thermal denaturation assay utilising a hydrophobic residue-binding dye which infers protein unfolding using qPCR (ThermoFluor I believe is the technique name).
Have you got any other suggestions? I'm still struggling somewhat with long term stability.
It was very helpful and easy. Thanks
Please share my channel link with your friends and juniors
very nice and point wise explination.Thank you a lot.
Please share my channel link with your friends and help me to reach big audience
What a great video. Thank you very much
Please share my channel link with your friends and help me to reach big audience
Thank you for this great video! Could you make a video about pI calculations of polypeptide chains?
sir can you please tell me why and how high molecular weight has less surface area and that leads to less hydration less salt required for precipitaion of protein in case of globulin which is know as half saturation and which is exact opposite for albumin that ppt in full saturation
Finally i understood..thanks
😅
nice explanation, thank you for explanation its very informative and helpful
however i have a question, regarding the protein isolation, if i not sure about the solubility strength of my desire protein, then salting in (addding low concentration of salt solution to allow it to solube is better) or salting out is better. And base on explanation, i understand that both salting in and out is helping to isolate, is there any condition like protein that not likely to soluble will be favor for salting out process? if there is, is the example fibrous protein will be more easier to seperate in salting out or salting process? Thank you for answering, look foward to knowing more on this
Please share my channel link with your friends and help me to reach big audience
I appreciate your work sir thanks 😊
Please share my channel link with your friends
I think with high molucal weight..we in are in case water in molecule. When the molecule is very low weigheit we are in case in molecule in water..
I think in hight salt the high molecule began to contract and push water in outside. We can see that in milk coagulation by elevating temperature..
I think all high molecular product can forme an emulsion
Water in oil or oil in water..
Thank you for video..👍👍🇩🇿🇩🇿
Very helpful, I understood the concept very easily. Keep up the good work!!
Please share my channel link with your friends and help me to reach big audiance
Thank you, your explanation was simple and to the point.
Please sharee my channel link with your friends and help me to reach big audience
sir plz give more detial how much saturated salt sol for specific purification which protocols is to follow for desire protiens
very nice and simple, thank you
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Thank you for this awesome video !!! It was awesome and helped me to understand this mechanism :)
Please share my channel link with your friends and help me to reach big audience
The video is useful, thank you
so helpful and so concise, thanks so much!
Please share my channel link with your friends and help me reach out to big audience
@@animatedbiologywitharpan for sure :)
loved it.. can you make a video on the change in protein conformation with the change in the ph of the solvent. Thank you!!!
I would try to make a video on that soon.......but please share my channel link with your friends and help me to reach big audience
Amazing videoooo
Please share my channel link with your friends and help me to reach big audience
Thankyou sir crystal clear concept 👍🙏
Please share my channel link with your friends and help me to reach big audiance
Also check out my profile in unacademy app there you would get more than 100 free of cost lectures and practice sessions
Yes yes I have already viewed many of your free lectures, very helpful thankyou so much.🙏
Wow well explained ♥️
great and clear explanation
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@@animatedbiologywitharpan I just wanted to take a moment to appreciate your incredible work. Your unique teaching style is truly refreshing and inspiring. You have a remarkable ability to present complex topics in such an engaging and accessible way. Your creativity and passion for educating others shine through in every video. Thank you for making learning enjoyable and for sharing your expertise with us.
thank you so much ! from France :D
Thanks for your comment...it helps me to stay motivated and make more videos
Please share my channel link with your friends and help me to reach big audience ...lots of love from India
This was helpful, thank you
Thank you so much for this amazing video
Please share with your friends and help me to reach big audience
Great video Arpan. Very helpful for my recreational study of org chem/biochem.
If you like the content please share among your friends and help me to reach big audience
Wonderful 💯💯
Please share my channel link with your friends and help me to reach big audiance
Finally got it thank you so much
Please share among friends and help me to reach big audience