first of all - thanks, it really helped me to understand several steps of this procedure better! secondly, it's the best unintentional asmr i've heard in my entire life, please, continue
Ahh this was such a joy to watch. I am currently doing my master's thesis on recombinant production of some protein and watching you do this whole process was such a cool experience 😂 I am so motivated now for my GST affinity purification tomorrow 😂💪🏼
@@user-zd7ns9ij5g and its characterization and potential application in serological test development. But yes, the central part of it was recombinant production of proteins. Sounds pretty underwhelming, right?
Hello! My name is Maria and I am a PhD student working on a project that works to collate various biological techniques for early career researchers in the lab. I really loved your tutorial video for protein purification, and was wondering whether we could get in touch to discuss it further and other similar protocols. Please let me know I'd love to hear back from you!
I do protein expression with 6 liters of culture. After centrifugation of the lysate, the supernatant is passed onto the Ni-NTA agarose column. It takes several days for the supernatant from such a large culture to pass through the column or immediately by syphoning the supernatant out of the column by applying a vacuum to the base of the column.
Please tell me how you made your elution buffer, everytime I add imidazole the pH rises above whats needed. And adjusting it with acid is not possible because of possible interference.
very good presentation well done,i have a question if you don't mind i which way should i adjust ph? my protein has PI=4,3 and i'm really confused if i have to regulate TRIS buffer ph to 6
The isoelectric point (pI) of your protein is the pH at which your protein is least soluble. If you want your protein to be soluble, your protein pH should be distance from the pI. Tris does not buffer to pH 6 because the pKa of Tris is 8.1 and pH 6 is two pH units away from the pKa of the buffer. Low pH elutes proteins from Ni-NTA Agarose, because it protonates the histidine residues in the polyhistidine tag and thus your recombinant target protein cannot bind to the resin.
thank you the videos ,i am doing sds page but my run wont start even after adding running buffer to the mark .i use the same mini gel tank like yours ,though it leaks ,could it be the issue ?help me from this confusion please
Use fresh running buffer, ensure you have the electrodes the correct way, ensure no leaks, make sure gel is entered the right way, remove the white tape at the bottom of the pre-case gel if you are using those
We use a Fisherbrand™ Model 505 Sonic Dismembrator (500W) set to 25% amplitude. (www.fishersci.ca/shop/products/fisher-scientific-model-505-sonic-dismembrator-4/p-3974677)
![[LIVE] : ONE ลุมพินี 69 | คู่เอก "กุหลาบดำ vs นาบิล"](http://i.ytimg.com/vi/PNM6U8e_HMo/mqdefault.jpg)
Please continue this, it's definitely a channel worth subscribing
I'm glad that I found your channel. It's really a nice and detailed Protein extraction video on TH-cam that's really helpful for beginners like me 😃
first of all - thanks, it really helped me to understand several steps of this procedure better!
secondly, it's the best unintentional asmr i've heard in my entire life, please, continue
All Biochemistry Master's Students looking to understand the method of protein expression for your project hands up ✋🏽
🙌
holler
🖐
Great video! Great technique. Thank you.
Great performance, great explanation. Thank you.
Good presentation with detailed explanation
Aabsolute master! Thanks my dude - this helped a lot! For the viewer's sake you might include on-screen stats for the reagents used. Thanks again! :D
Hi, this video was great!! Maybe you can do additional videos on SDS-page interpretation (maybe with different proteins and conditions).
Thank you so much for this!
Thanks for the video. As a first-year PhD student, I needed this video to get my feet on the ground. Thanks a lot
Thank you very much, this is great for teaching with limited lab.
Thanks a lot!! Very good explanation
I liked the cotton idea :) on gel staining part of the protocol. Useful indeed
Outstanding sir
Really good and well explained 👍
So helpful, thanks a lot!!
Thanks a lot!!
love this video thank you!!
Ahh this was such a joy to watch. I am currently doing my master's thesis on recombinant production of some protein and watching you do this whole process was such a cool experience 😂 I am so motivated now for my GST affinity purification tomorrow 😂💪🏼
Your whole MS thesis was on recombinant protein production?
@@user-zd7ns9ij5g and its characterization and potential application in serological test development. But yes, the central part of it was recombinant production of proteins. Sounds pretty underwhelming, right?
not really, some proteins are difficult to purify, talking from experience, especially uncharacterized proteins. @@aleksandar2046
Hello! My name is Maria and I am a PhD student working on a project that works to collate various biological techniques for early career researchers in the lab. I really loved your tutorial video for protein purification, and was wondering whether we could get in touch to discuss it further and other similar protocols. Please let me know I'd love to hear back from you!
I appreciated the level of detail you described here, thanks. Using your technique, about how long does it take you to grow and purify a 1L culture?
Hi Great Job
Can I have this protocol in written form So i cannot miss any point.
It would be highly appreciated
How do you determine what gradient of SDS-PAGE gel to use since there are multiple products on the market that range from 4%-12, or 4%-20% gradient?
Thank you !!!
I do protein expression with 6 liters of culture.
After centrifugation of the lysate, the supernatant is passed onto the Ni-NTA agarose column. It takes several days for the supernatant from such a large culture to pass through the column or immediately by syphoning the supernatant out of the column by applying a vacuum to the base of the column.
Use a 5 mL HiTrap with an FPLC system
Quality content
thank you
I want to see how the running gel looks like.
Hello protein purifiers, hahahaha
Thank you
very nice
Please tell me how you made your elution buffer, everytime I add imidazole the pH rises above whats needed. And adjusting it with acid is not possible because of possible interference.
Do you have a protocol please
Can you share some references you use to do in this video? Thank you so much
whats the name of the spectrophotometer machine you were using?
What is the success/trial ratio of this process, if i follow the process exactly, will i be able to express the protein or does it takes few trials
U have used dnase before sonication.. does the addition of dnase before sonication impacts lysis differently or it does not affect the lysis
It is always better to add lysozyme AFTER resuspending the pellet.
please write the name of manufacture Ni-nickel resin
Which protein you are expressing and purifying
May I know what does it mean by to wash with 10 column volume? I encounter this in an article
By "column volume" I'm referring to the volume of resin inside the column, so if there is 1 mL of resin, 10 column volumes is 10 mL.
very good presentation well done,i have a question if you don't mind i which way should i adjust ph?
my protein has PI=4,3 and i'm really confused if i have to regulate TRIS buffer ph to 6
The pH of the buffer depends on your protein, but for binding to Ni-NTA it needs to be between 7.5 and 9.
The isoelectric point (pI) of your protein is the pH at which your protein is least soluble. If you want your protein to be soluble, your protein pH should be distance from the pI. Tris does not buffer to pH 6 because the pKa of Tris is 8.1 and pH 6 is two pH units away from the pKa of the buffer. Low pH elutes proteins from Ni-NTA Agarose, because it protonates the histidine residues in the polyhistidine tag and thus your recombinant target protein cannot bind to the resin.
Hi where is your lab I have a few questions
thank you the videos ,i am doing sds page but my run wont start even after adding running buffer to the mark .i use the same mini gel tank like yours ,though it leaks ,could it be the issue ?help me from this confusion please
Use fresh running buffer, ensure you have the electrodes the correct way, ensure no leaks, make sure gel is entered the right way, remove the white tape at the bottom of the pre-case gel if you are using those
LB should be pH'd to 7.
what dose the energy you use for sonicator the bacteria?
We use a Fisherbrand™ Model 505 Sonic Dismembrator (500W) set to 25% amplitude.
(www.fishersci.ca/shop/products/fisher-scientific-model-505-sonic-dismembrator-4/p-3974677)
Where is the lab situated?
We're in Montreal
10:40 for Day 4
Is this how you can make human growth hormone?
Maybe this reference helps:
Olson, K.C. et al. (1981) Nature, 293, 408-411
doi.org/10.1038/293408a0
@@kwanlab4034 ? Link doesn’t work. How to make HGH?
how do you bring the filming apparatus into the lab? With parafilm covered?
It's just a cell phone camera and a tripod.
Is the ice important and why?
To avoid protein degradation