Thanks for the video, it was really clear. I do have a question when you pause the video on 05:38 you can see a few nucleotides that don't have any peak. Do you find this trustworthy? Let's say we take the second T after the substrate peak, it hardly peaks at all, while the one before has a very nice peak. I hope you can answer my question because I'm new to using pyro and am trying to figure out why they still say it is that nucleotide instead of another. Thanks in advance.
Hi Amber, sorry I rarely check this video so I appreciate this answer is probably now of little use to you. The lack of peak on the second T you mention is meant to be peak free as its acting as a "soak up" T. All the T at this position should be captured in the initial peak, if not, its captured in the second. This prevents bleed-through in the remaining sequence that would ruin the accuracy of the results.
Thankyou for your help on this challenging procedure
Thanks for the video.
Do you know how to run a self test and calibration
Thanks for the video, it was really clear. I do have a question when you pause the video on 05:38 you can see a few nucleotides that don't have any peak. Do you find this trustworthy? Let's say we take the second T after the substrate peak, it hardly peaks at all, while the one before has a very nice peak. I hope you can answer my question because I'm new to using pyro and am trying to figure out why they still say it is that nucleotide instead of another. Thanks in advance.
Hi Amber, sorry I rarely check this video so I appreciate this answer is probably now of little use to you. The lack of peak on the second T you mention is meant to be peak free as its acting as a "soak up" T. All the T at this position should be captured in the initial peak, if not, its captured in the second. This prevents bleed-through in the remaining sequence that would ruin the accuracy of the results.