Pyrosequencing - The Basic Principle and Steps Involved Explained

Pyrosequencing

[@Siyakunar097]

I just want to say this thing.please continue making these videos you might have no idea that your channel is the best for clearing concept.
So simplified way of presenting.
Plz continue them

[@maddsfogs369]

Thanks heaps! This is the best comprehensible explanation I've come across

[@AZ-qx1xd]

This is such a high-quality chanal, amazing work! Your videos have helped me out often!

[@asmaakterbhuiyan_shanu3951]

Amazingly explained! The concept is now crystal clear, would please make video on Next generation sequencing and microarrays?

[@jesigibbs6741]

Extremely Helpful! Thank you!

[@yyl5336]

Thank you very much. You made the process really easy to understand.

[@reshmasinha379]

Well explained, appreciate the effort. Thank you.

[@sadiyaanjum1001]

Your lectures are so good..really appreciable

[@ghanirehman5116]

Well explained, appreciate the efforts. Thank you

[@tamanamsudhir4576]

You are a life saver. Thanks.

[@Tiger-cm1qc]

Great breakdown; well done.

[@ifyifemanima3972]

Thank you for making this video. Brilliant!!!!

[@priyahalder2830]

It was helpful... thank you for explaining in such a easy way

[@shankhamitrapaul6370]

Thanks a lot.You made it realy realy easy for me to understand.Before this I found i taugh.respect from Bangladesh🙋

[@entertainmentworld3397]

Really Amazing and exceptional lecture

[@vijayalakshmiprakash5720]

thank you for the amazing explanation, it is very clear

[@bezayitamare6180]

An Excellent explanation. Thank you.

[@rikkigupta9546]

Thanks a lot your all lectures are very informative 😇

[@safwatali8850]

Fantastic. Best wishes.

[@hohohehe7864]

Perfect, thank you sooooooo much
That helped me alot for my exam

[@manishpathak2710]

excellent work👌👌👌👌

[@djazmit8321]

Amazing explanation thank you so much

[@nibirrahman2332]

Super simplified. Thanks

[@aanjalithillainathan9295]

thank you so much, would be helpful if you uploaded a video on 454 pyrosequencing

[@nousheenbaloch6951]

Very helpfull, thank you !👌

[@khizarhayat7123]

Amazing lecture thank you

[@lilithasotondoshe8226]

This is awesome thank you so much

[@alakeshpal9509]

Very Helpful.Thank You

[@rayhanmahfuzreet7131]

Outstanding!

[@venko6]

Great video, thank you so much! Maybe it is worth checking the enzymes and substrates showed in the box at 4:24 minute - there the Luciferase is listed twice. Is this intentional?

[@ImranAhmad-df3mi]

Nyc explanation ever

[@itsmybio8645]

Best Video.. Thanks

[@snehadas2159]

Thank you mam for this lecture 😌

[@aidah6381]

Awesome, thank you

[@ngocanhvo8957]

It is really helpful :)

[@kunuyashodha7974]

Comprehensive thank you

[@bianzatinoembavha2451]

Very helpful, thank you

[@riddhimanchatterjee4195]

Greatttt! Thanks a lot 😁

[@asuramunikavishadesilva371]

Thank you!❤

[@iman_ktb]

Really helpful thank you

[@hirva362]

Very nicely explained

[@zaydoon962]

That was helpful thanks! But how is this applied in instruments?

[@SaravananS-gc4kt]

Wonderful thank you mam ✨

[@tshabalaladimphojeanette9969]

Life saver 😭🙏❤️🕯

[@rajeshkumarsattenapalli5085]

Thank you very much

[@user-lw4fv8ql3j]

Very nice video

[@duaakhalid9603]

Amazing>> please , What is the name of program that using for making these videos. thanks

[@marwaelhadidy7831]

Excellent

[@ashirt2100]

Thank you so much.Your videos are very helpful. I follow your channel regularly.I must appreciate your hard work.Very simple and clear explanation.Can you make videos on Next Generation Sequencing and biosensors?Please try.Thank you again.

[@FrankLectures]

Thank you for your valuable feedback.
The topics suggested by you will be added soon. Thanks

[@ashirt2100]

@@FrankLectures Thank you so much ...:)

[@nrehmankhan5334]

Amazing

[@sohanursohan6425]

great!!

[@ArifulIslam-yt5df]

thanks a million

[@medicalbiochemistry_]

Nice👍

[@leilasaadi5952]

Thank you.

[@gon505]

Can you make video on maxam gilbert dna sequencing

[@niloofarkh4779]

TAHNK U SO MUCH

[@ashirt2100]

Hey!!!!! Why are not uploading new videos????

[@fidamuhammadkum]

mam please discuss the DNA library for pyrosequencing

[@sautrikbhattacharya]

Is apyrase added at each step? If so then how is it removed or inactivated before the addition of next nucleotide??

[@moqaddasbashir4034]

Yes it is necessary to add apyrase at each step to assure that every extra nucleotide and as well as the ATP is degraded.
According to my knowledge apyrase can only degrage unbound nucleotides and the bound nucleotide are not degraded by it and if apyrase is not degraded then I think it has no problem for boun nucleotides

[@MuhammadIslamag]

@@moqaddasbashir4034 but it can degrade the coming nucleotide that are not added still. How will u justify

[@worldofmicrobiology4535]

@@MuhammadIslamag the rate of polymerization by polymerase enzyme is faster than the rate of degradation done by apyrase enzyme

[@thippesha4615]

why dATP(alpha)s is added?? if anyone knows please reply with answer

[@ShahdSamer-qc4nq]

gama3a shofoha wallahy fashekha

[@devarshkumar9955]

what about beads? Biotin? magnetic beads? PCR? Wells where it is loaded

[@sreeram6416]

Maam will it help in cracking jam exam

[@rtkayush210]

No videos from last 2 years

[@krishnaraj7612]

10th new syllabus English medium types of fossilizalion explanation please send me videos

[@mubeenajasif8775]

Mubeena

[@056vatsalapandey3]

are you god or what

[@wowalamoiz9489]

As usual, another good channel stops uploading...

[@safaashehab9662]

Very helpful, thank you

[@alliswell4117]

Excellent

[@Anmolkumar-yx1bo]

Very nicely explained

[@imenebenamira2851]

thank you so much I've been looking for such an explanation for a long time.