Does it matter if RO/DI water is used as a blank? Or if an empty cuvette is used as blank. I think I've seen cases where a wavelength is set and than zeroed with no cuvette.
Hello Isaac! I hope this helps. Reverse Osmosis (RO) is often used to partially clean-up tap water to make it roughly 90% to 99% pure. Deionization (DI) filters exchange positive hydrogen and negative hydroxyl molecules for positive and negative contaminant molecules in water. I think it is better to use DI Water. Setting the wavelength to zero is different from setting the ABSORBANCE to zero. Hence, in setting the absorbance to zero you need a cuvette containing either your DI water or a sample or reagent blank. :)
I wish every guide in the world was done as clearly as this. Thank you!
Thank you so much. Nice illustration
Nice demonstration 👏🏻
Thank you so much for this! Very helpful! Grabbed it to show my class.
Hallo ... Thanks for your video, it help me for remembering how to use it... It help me for my research 😂
Can this be used to measure cell density or OD600 at 600nm ?
Does it matter if RO/DI water is used as a blank? Or if an empty cuvette is used as blank.
I think I've seen cases where a wavelength is set and than zeroed with no cuvette.
Hello Isaac! I hope this helps. Reverse Osmosis (RO) is often used to partially clean-up tap water to make it roughly 90% to 99% pure. Deionization (DI) filters exchange positive hydrogen and negative hydroxyl molecules for positive and negative contaminant molecules in water. I think it is better to use DI Water.
Setting the wavelength to zero is different from setting the ABSORBANCE to zero. Hence, in setting the absorbance to zero you need a cuvette containing either your DI water or a sample or reagent blank. :)
Hi! this is totally random, i’m from indonesia!
Ogheyy🙂