DNA Quantitation Using a Spectrophotometer
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- เผยแพร่เมื่อ 23 ธ.ค. 2024
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This video demonstrates how to quantitate DNA using a UV-capable SmartSpec™ spectrophotometer. Plasmid DNA is measured, showing step-by-step instructions for the instrument. Purity of the DNA is assessed using the A260/A280 ratio.
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thanks, also nice matching green buttons, gloves and tube racks: outstanding attention to detail.
Hj
Very helpful...was looking for such vedio for a year
Thanks
by OD rule of DNA, 1 OD=50 ug/ml. then it should have been 0.55 ug/ml. any reason for this minor variation?
I thought for DNA 1.8 is the perfect purity and for RNA it is 2. Why is it so high here?
I looked into it and it seems like you have RNA contamination in your DNA sample?
same doubt
@@zauberdiva17 yes, I think you're right. The sample shown is contaminated.
Very helpful for freshers
how do you do that?
11 years ago😮
Thank u for uploading good video
Thank you! ❤️
12 Years Ago 😮
Good
Please send write up sir
8 years ago
Wtf
11 right now
12 right now
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