The Science Behind UV Vis Spectroscopy: Everything You Need To Know
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- เผยแพร่เมื่อ 26 มิ.ย. 2024
- Welcome to our educational video on UV Vis spectroscopy! In this tutorial, we will explore the fascinating world of UV Vis spectrophotometry and its working principle. Using the example application of caffeine concentration determination, we will demonstrate how UV Vis spectrophotometers can be utilized in practical scenarios.
UV Vis spectroscopy is a powerful analytical technique for analyzing the interaction between light and matter. It helps us understand the absorption and transmission of ultraviolet (UV) and visible (Vis) light by molecules in a sample. By measuring the absorbance or transmittance of light at different wavelengths, we can gather valuable information about the chemical composition and concentration of substances.
In this video, we will walk you through the step-by-step process of using a UV Vis spectrophotometer to determine caffeine concentration. You will learn about sample preparation, instrument calibration, wavelength selection, measurement techniques, and data analysis. By following along with our demonstration, you will gain a clear understanding of how UV Vis spectroscopy works in real-world applications.
Whether you are a chemistry student or a scientist looking to expand your knowledge of analytical techniques, this tutorial is designed to provide comprehensive insights into UV Vis spectroscopy. By the end of this video, you will have a solid foundation in understanding the working principles behind UV Vis spectrophotometers and their practical applications.
Subscribe to our channel for more informative videos on analytical techniques, and stay tuned for future content exploring various spectroscopy aspects. Take advantage of learning how to harness the power of UV Vis spectroscopy in your scientific endeavors!
Learn more about the basics of UV Vis Spectroscopy by downloading our fundamentals guide here:
www.mt.com/global/en/home/lib...
You can obtain more information about our spectrophotometers, including the UV5, UV7, UV5Bio, and UV5Nano, here:
www.mt.com/global/en/home/pro...
0:00 Introduction
1:09 What Is a Common UV/VIS Spectroscopy Application?
2:05 How Does a Spectrophotometer Work?
2:56 How Can We Determine the Concentration of Caffeine in a Sample?
4:25 How Does a Micro-Volume UV/VIS Spectrophotometer Work?
5:44 Let Us Tell You about METTLER TOLEDO Spectrophotometers - วิทยาศาสตร์และเทคโนโลยี
loved this video! Good explanation. Thank you!
Thank you for watching!
Thank you very much for this great and funny explanation :)
Glad you liked it!
Thank you. Good explanation. Love the clarity
Thanks for watching
Nice explanation and illustration. These products must be awesome.
They are!
Thanks for making this thing look simple..really learnt a lot..tnx
Most welcome 😊
Great video! Helped me so much in my chem assignment!
Great to hear!
Great video!
Thank you
nicely done, perfect Job, wish you the beset
Thank you very much!
Amazing explanation
Glad you liked it
really appreciate your way of explanation
Thank you so much for your kind words!
Are there machines that can measure absorbance as a function of temperature? Like say you are working with something that changes color when heated or cooled
Depending on the application we would recommend the CuveT Accessory in Combination with an UV/VIS Excellence Instrument in order to determine such thermochromic behavior. For more information or to get in direct contact please check: www.mt.com/global/en/home/perm-lp/product-organizations/ana/thermostating-uvvis-peltier-temperature-control.html
Thanks a lot ma'am this was an amazing explanation
Most welcome 😊
Most welcome 😊
Nice one application based knowledge here
Thanks for watching
Thanks
Welcome
hello! if you don't mind replying asap, how do you determine the pre-programmed calibration curve? where do you get that from?
There is no pre-programmed calibration curve available. The method requires measuring the first 6 caffeine standards (0, 2, 4, 6, 8, and 10 mg/L). Afterward, the calibration curve is determined and stored in the instrument. Please feel free to contact your local MO for further questions. Contact details can be found at www.mt.com under "Contact Us".”
Can I measure the concentration of (Benzene , Toluene ,Ethylbenzene) in water ?
If I can do that , please tell me about the price.
with appreciate
Thank you for your question. Yes, theoretically, it is possible, because all these three organic compounds contain an aromatic ring in their chemical structure, which has strong absorbance in the UV light region and thus can be detected by a spectrophotometer. However, in practice, please note that the solubility of these organic compounds is very low. When not all is dissolved in water, the undissolved will be floating on the top of water and concentration determination is not correct. On the other hand, if the concentration is extremely low, e.g. only several ppb, the detection limit of the used spectrophotometer may affect the analysis feasibility, because the signal received by the spectrophotometer may be too weak to generate an accurate result. If these three organic compounds are mixed in water, separation is required before using spectrophotometry to determine the concentration of individual components in the mixture. For further evaluation we would higly recommend contacting your local sales representative.
Great! But what is the difference between AAS/FES and the UV/VIS? both give the concentration…
Dear Jana Zadro,
AAS (atomic absorption spectroscopy) and FES (flame emission spectroscopy) are analytical techniques used to determine the concentration of elements in a sample. In both AAS and FES, a sample is atomized and the resulting atomic vapor is excited by a flame or other energy source. The excited atoms then emit light at specific wavelengths, which are characteristic of the element being analyzed. The intensity of the emitted light is measured and used to quantify the concentration of the element in the sample.
UV/Vis (ultraviolet-visible spectroscopy) is a technique used to measure the absorption of light in the UV and visible regions of the spectrum. It is commonly used to quantify the concentration of chromophores in a sample, which absorb light at specific wavelengths. The absorption of light by a sample at a particular wavelength is related to the concentration of the absorbing species in the sample.
The main difference between AAS/FES and UV/Vis is that AAS/FES measures the emission of light from an excited sample, while UV/Vis measures the absorption of light by a sample. Additionally, AAS/FES typically requires more specialized equipment and expertise to perform compared to UV/Vis.
Why does the light need to be diffracted before it’s directed to the detector?? Thanks
This is a typical setup you will find in array spectrophotometers.
As the light passes through the diffraction grating, each wavelength is diffracted at a slightly different angle, causing the different wavelengths to separate from one another. The detector then measures the amount of light at each specific wavelength, allowing us to determine the absorption or transmission spectrum of the sample.
You can find a more detailed view in this video: th-cam.com/video/XAp-5r3LxQo/w-d-xo.htmlsi=TeFagcZ18dr-eVdN&t=23
Can it determine the chlorine amount in the water?
Yes, it can. Please see this video
th-cam.com/video/fZV2O1ov8Yo/w-d-xo.html
How about the powder and thin film?
Hi,
With our solid sample holder, you may analyze thin film.
Which of those devices can analyze soil samples?
Hi Servare,
With the appropriate sample preparation, soil samples can be analyzed with all cuvette-based UV/VIS Excellence spectrophotometers (UV5, UV5Bio and UV7) e.g. for organic substances or inorganic ions. For more information or application support, please contact your local sales partner, contact details can be found at www.mt.com under "Contact Us".
Soilgauge