Wow, this is awesome! Makes sense after watching doing it in practice. I would like to try it out... Though, it seems like it is a very long procedure? Several days to finish?
you probably dont care but does anybody know a way to get back into an instagram account?? I was stupid lost my login password. I would appreciate any help you can offer me.
@Apollo Lane Thanks for your reply. I got to the site through google and Im in the hacking process now. Takes a while so I will reply here later with my results.
Why is NaCl used in alkaline and neutralization buffers? How does NaCl play role in dna denaturation? Will neutralizing the solution not rejoin the dna strands?
NaCl in alkaline and neutralization buffers increases ionic strength, aids in protein precipitation, and stabilizes DNA in solution. NaCl stabilizes the DNA double helix, making it more resistant to denaturation in high salt conditions. Neutralizing the solution can allow for reannealing of DNA strands, but the efficiency depends on DNA size, concentration, and buffer conditions. During plasmid isolation, plasmid DNA reanneals more efficiently due to its smaller size and supercoiled nature compared to fragmented genomic DNA
what a good video for southern blot. By the way, Chinese lab is so kind to public their techniques to the whole world for free of charge. It is kind of the lab.
An okay attempt at a video, but it's sooo boring and amateurish. I'll try to remember to avoid Abnova when I need reagents for an experiment whose data I'm going to publish publically....
maybe you should know about about the basics before commenting on it and this technique is not what is used these days.......Real-Time Quantitative PCR is used now, which you may notice is how covid is detected
@@swiss4ever93 Well we are primitive but not in the way you are thinking. Don't trust me just start searching on the internet about the underlying principles of these very primitive technologies you just complimented.........
@@lumieredice485 I wouldn't say outdated, I am sure there are many specific tasks it is still used for, I just don't know. Maybe It's the same as how they teach us all the atomic theories like Thomas, Rutherford, Bohr's atomic models before moving to what is more acceptable today. Some prior easier, maybe outdated/faulty stuff can help in teaching the stuff that's more advanced. A bit of history lesson hidden in all the science to honor those who spent their life for it. Or the fact that we are not ready yet to understand the more advanced voodoo magic. Maybe a little bit of everything, maybe neither of it and I am just rambling nonsense. I don't know 🤷♀, I just like talking too much.
I just came back for the background music.
Lmao no lie
Nice video. Pls show the coloured DNA fragments too.
Can you show the results please?
This technique was the precursor to much fancier technology now called micro arrays...Thank you Edward Southern.
Is there no addition of Detection buffer at last?Am curious to see the results for this.
catchy beats yo
To be perfect just needed to show the results at the end
Yes I used gel electrophoresis for proteins.
Wow, this is awesome! Makes sense after watching doing it in practice. I would like to try it out...
Though, it seems like it is a very long procedure? Several days to finish?
you probably dont care but does anybody know a way to get back into an instagram account??
I was stupid lost my login password. I would appreciate any help you can offer me.
@Christopher Otis instablaster ;)
@Apollo Lane Thanks for your reply. I got to the site through google and Im in the hacking process now.
Takes a while so I will reply here later with my results.
@Apollo Lane It worked and I finally got access to my account again. I am so happy:D
Thanks so much, you saved my account :D
@Christopher Otis Glad I could help :D
Why is NaCl used in alkaline and neutralization buffers? How does NaCl play role in dna denaturation? Will neutralizing the solution not rejoin the dna strands?
NaCl in alkaline and neutralization buffers increases ionic strength, aids in protein precipitation, and stabilizes DNA in solution.
NaCl stabilizes the DNA double helix, making it more resistant to denaturation in high salt conditions.
Neutralizing the solution can allow for reannealing of DNA strands, but the efficiency depends on DNA size, concentration, and buffer conditions.
During plasmid isolation, plasmid DNA reanneals more efficiently due to its smaller size and supercoiled nature compared to fragmented genomic DNA
Background music on 1,5x is something else
A bit curious since there are quite a good educational youtube video, is there a way to cite them in a paper lol?
what is hybridization probe of abnova that i can use to do like this video
How do I find the song in the video? The description doesn't help me find anything
gel electrophoresis can't be used for protiens SDS PAGE is used for proiens
where is this song from!!!??
Ngl I enjoyed the background music
Great practical sir/Ma'am. Really helpful for practical hand.
After blotting What next
What happens next!!!!
all dat and i still seein a blank piece of paper????????/
*cough* autoradioscopy *cough*
what a good video for southern blot.
By the way, Chinese lab is so kind to public their techniques to the whole world for free of charge. It is kind of the lab.
Nice video.
Also, I like the choice of music : ]
After autoradiagrahy What nex
Perfeito, vendo na prática é muito mais fácil de entender
thank you too
what next :( ?
I don't even know this shit but this was a great video
I love experiments this video is useful for all the students ...
Nice video! Great experience!
I did this with proteins...
Western blot? :D
@spongenooob I agree!! :D
online labbing in 2020;(
🙂🙂🙂🙂
Gosh! Sooo long
Tutto questo per cosa?
কিচ্ছু বাল ই বুঝি না😑😑😑
An okay attempt at a video, but it's sooo boring and amateurish. I'll try to remember to avoid Abnova when I need reagents for an experiment whose data I'm going to publish publically....
Science is still so primitive, all this just to see DNA??? and we wanna fly to mars🤣🤣
maybe you should know about about the basics before commenting on it and this technique is not what is used these days.......Real-Time Quantitative PCR is used now, which you may notice is how covid is detected
@@sukhchain9696 and we're doing so great with covid, thanks for emphasizing my point
@@swiss4ever93 Well we are primitive but not in the way you are thinking. Don't trust me just start searching on the internet about the underlying principles of these very primitive technologies you just complimented.........
@@sukhchain9696 interesting...so my class is basically giving out outdated information and links...
@@lumieredice485 I wouldn't say outdated, I am sure there are many specific tasks it is still used for, I just don't know. Maybe It's the same as how they teach us all the atomic theories like Thomas, Rutherford, Bohr's atomic models before moving to what is more acceptable today.
Some prior easier, maybe outdated/faulty stuff can help in teaching the stuff that's more advanced. A bit of history lesson hidden in all the science to honor those who spent their life for it. Or the fact that we are not ready yet to understand the more advanced voodoo magic. Maybe a little bit of everything, maybe neither of it and I am just rambling nonsense. I don't know 🤷♀, I just like talking too much.