In Situ Hybridization

[@manarsmaan2624]

This is how the person explain when he loves what he does .
thank you so much for the great explanation
All Respect

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@renegerritsen6178]

This is the third video I watch on this topic and you are the first to actually make it understandable, thank you!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@mireillet4573]

This is what you call a good and simple tutorial. Thank you so much !

[@shomusbiologyofficial]

You're welcome

[@wrapupstudio9149]

you are tooo good.... A teacher like you is not even in reputed colleges... thank u so much.. keep going

[@maryamjokar7066]

4:10 now that was a perfect circle 😳

[@shomusbiologyofficial]

Thank you

[@umeaiman4168]

Sir, u r one of my great teachers...my every problem has solution from ur lectures...

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@malikamehmood3281]

Tomorrow is my exam, and you know what you are a great teacher and understand each and every thing you've explained, thank you so much ❤️

[@mmmmmmmmm4383]

Thank you for appreciating his efforts

[@stephanie_4203]

This is awesome. Why I can't find teachers like him¡¡ I understood all¡¡¡ Thank you so much. I will approve my exams now¡¡¡

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@muskangarana8074]

You are really master of microbiology
Thanks a lot for the videos 🙏

[@shomusbiologyofficial]

You're welcome

[@xeverydaynormalgirlx]

My favourite part of this video was the part where an ad popped up offering to do my assignments for me. I bet that will help my learning.

[@yaseenkhan-im9ti]

Aap jaise logon ki wajah se genetics interesting hai

[@shomusbiologyofficial]

Thank you

[@SunandGold]

Thank you!! Really helpful, I was so confused by all the websites I used because they over-complicated the terminology and the diagrams! I spent so much time on those websites getting confused, when I could have just watched your video

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@rakshithav2271]

Thank you so much... trying to prepare for exams. And your videos make them so much easier.

[@chintanmehta4695]

Hats off sir! I am speechless that the way you narrate is amazing one.

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@yasirahmad2185]

Sir, i m pakistani and loves u sir for ur simplicity and teaching method. InshAllah in future i will come to india specially to meet u sir.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

[@jijizali]

NOBODY EXPLAINS ABT FISH BETTER THAN THIS VIDEO OF YOURS SIR THANK YOU SO MUCH

[@shomusbiologyofficial]

You're welcome. Glad you like it

[@hesnanoussa6442]

i do study biology ( biochimestry )but in frrench luckely ur explanation was quite good i have understood quite well

[@nyctophile1841]

Amazing way of explaining sir.. Thank you sir for this 🥰

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@coconuttree1972]

Sir, You're really something.... ❤🎉🎉🎉

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@CelestialCookies]

God bless you. You make biology as simple as it should be. Thank you very much

[@ushakarreddy1981]

excellent sir ..my doubts were cleared by your wonderful lecture..

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@diegonzalez25]

Muchas Gracias Shomu!!! Saludos desde Paraguay

[@seepa3890]

It is easy because you explained it in simple way☺️

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@khushshah524]

I am presenting this technique tomorrow in front of my PI. This guy saved my life. I have a question: what does hybridization mean in HCR? Which step in this process is
called hybridization?

[@anj19911]

AMAZING!! BETTER THAN MY SCHOOL TEACHERS!!! THANK YOU!!!!!!!!!

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures. Please subscribe and share

[@chhoamondal3526]

I have understood easily the complex thing from your lecture

[@shomusbiologyofficial]

You're welcome

[@DHIRAJKUMAR-xv4sr]

Helpfull for me all six lecture related to gene mapping......thanks sir .😊

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@shwetagupta9004]

Thank u so much sir . Aap n hote to pata nahi hum students ka kya hota.

[@shomusbiologyofficial]

You're welcome

[@briabriapia]

dam that circle was perfect

[@yimuyang4101]

Thank you Shomu!! Your lecture make it easy to learn, Big help!!!

[@sramanagiri3803]

Ur explainations are so worthy to listen

[@fikayoadegboyega3580]

Nice one. I so much like your teaching skills

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@RajKumari-ld7ld]

Thanks brother.... U r a brilliant teacher

[@RahulKumar-mm5vm]

I have a question regarding dna sequence using FISH. "Is it possible to identify a particular gene sequence (for example - Amel Y) using FISH if there is lot of depurination (loss of adenine , guanine) in interested gene sequence ?"

[@shikidixit5144]

Thank you, Shomu!

[@mayukhroy5393]

Wonderful explanation Sir.

[@shomusbiologyofficial]

You're welcome

[@tatyanakiryutina7778]

Thanks! Most helpful information I found so far!

[@shomusbiologyofficial]

You're welcome

[@emilymarques6739]

Thank you Shomu!

[@shomusbiologyofficial]

You're welcome

[@biologywithbasira]

great job! your explanation is amazing

[@shomusbiologyofficial]

Thank you

[@latimerpaul]

Great video, Shomu. Thank you!

[@shomusbiologyofficial]

You're welcome

[@mahyahosseinib.4366]

Many thanks, Shomu!!!

[@alonir101]

Thanks man, you are great and i like your passion about this :)

[@mansiwadekar1719]

Thank you
Very well taught

[@shomusbiologyofficial]

You're welcome

[@prathameshdeshmukh5844]

REALLY HELPFUL....... AWESOME LECTURE.... TY..

[@shomusbiologyofficial]

Glad it helped

[@NehaSharma-hd2so]

U r gem to me and others too.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@iancuharghel8532]

I hope you are doing fine! You are great!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from

[@AnimeUni-versed]

You are a blessing !

[@shomusbiologyofficial]

Thank you

[@zunairaakram1672]

Thank you shomu...helped me alot,,, cheers from pakistan... :)

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@giovannaagnelli6752]

Thank you so much! It was so very hard to find a good video about in situ Hybridization. 🍀🔬📚💘

[@mahaswetaguha-u4m]

You r very helpful bro

[@kouapatrick3838]

Thank you Shomu! I would like to know how the 2 strands of the DNA is separated in this process? by heating the cell ?

[@ruthzomuansangi9698]

KOUA Patrick i think nick translation is used.

[@danielhain40]

PLEASE MAKE MORE!!!

[@shomusbiologyofficial]

Sure

[@arias.p4355]

Thanks a lot! Your videos are amazing!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@jim5400]

Great job Shomu!

[@aishasoliman8647]

really helped!! thanks a lot
I have a question:
If I have wild type and mutant models and I wanna examine the expression of specific markers Should I prepare probes for each markers for both mutant and wild type ?

[@SMS.WAHEED]

Awsome sir but how know the gene sequence?

[@moonsevinci88]

Thank you. Plz how we can separate the strands ? in vivant

[@dasachomova201]

Thank you, all your videos are very helpful :)

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@noorqadri1690]

do one about WISH

[@me6d298]

Plz add a video on MLPA.

[@dr.bidouranaznin1339]

Thanks! nice & simple way to learn. How much work area is required for FISH setup?

[@markj.7687]

FISH set up is basic and does not require much bench top space. a simple table top would work just fine.

[@mohammadnamarneh2345]

You are amaizing 🌷🌷🌷🌷🌷🌷

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@arshiarshi3756]

You are right 👍 it is a easiest method.

[@sapirshaba7546]

Hello, can you explain about detecting abnormal chromosomes such as 18,21, by FISH?

[@supriyaubasupriyauba3217]

Thank you so much sir

[@shomusbiologyofficial]

You're welcome

[@SadikKhan-he8sc]

Plz make a video on random priming

[@HCLpradeep2010]

Superb Boss!

[@shomusbiologyofficial]

You're welcome

[@prachikamble1004]

Good explanation skills! I like ur all the videos!

[@nehanasir3492]

How is the complementary strand to the target sequence removed before hybridization?
Because it may happen that during hybridization or cooling, the target strand again bind to the complementary sequence instead of probe

[@lysin210]

Thank you ❣️

[@shomusbiologyofficial]

You're welcome

[@davidcharles6518]

Thanks very helpful👍

[@ShahFahad-eo6fm]

It was very helpful bro ,thank you for making the video

[@ci7672]

Thaaank u a lot teacher

[@shomusbiologyofficial]

You're welcome

[@soumyasudhi3955]

Useful video....Thank u..

[@oinambobochandsingh89]

Wonderful

[@shomusbiologyofficial]

You're welcome

[@thisumamarasinghe8171]

thnks ❤

[@shomusbiologyofficial]

You're welcome

[@rahishaaz4392]

Sir is FISH technique used for serotonin binding receptorss???
Plzz answer

[@normadicn5700]

Google?

[@chelseagunaratna5249]

Hi Shomu! You are amazing!

[@alemranhosen1531]

Love you from BD🇧🇩

[@shomusbiologyofficial]

Thank you

[@saimairshad5074]

Sir, is this same as southern and northern blotting techniques?

[@rashmisingh1857]

As i am pursuing PG.....wanna more elaboration of topics.......it wz lil bit helpful

[@shivanisehra1487]

Mere exams h next month please ap kuch topics h unpe video bna skte ho plzzzzzzzzzzzzz mujhe smjh nhi aa rha kuch

[@kirtisharma8609]

Tysm🤓😊great video...👍

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@danielnguyen123]

THANKS SHOMU ;) ❤️❤️

[@shomusbiologyofficial]

You're welcome

[@KIRUBAKAR]

Bro what's the difference between colony hybridization and insitu hybridization

[@totallyomi]

Sorry if this is a stupid question but how do we know the DNA sequence we are targetting in order to design the complimentary probe?

[@shomusbiologyofficial]

Dna sequence must be known prior to the experiment

[@vipanbisht4227]

Sir pls make videos on class 12 biotechnology chapters ......pls sir it's a humble request

[@shomusbiologyofficial]

Okay

[@mauliksadhu8963]

Is it not same like southern blotting...?
What's the difference between these two..?

[@gowhariqbal9323]

Sir u r really 👍

[@shomusbiologyofficial]

Thabk you

[@priyankatiwari3434]

thanks .....great video as always..

[@rajshreepal6636]

thank u sir.....

[@shomusbiologyofficial]

You're welcome

[@harshithan.jadhav5294]

Thank you so so much ✨

[@shomusbiologyofficial]

You're welcome

[@reviewit9100]

Very helpful video for mee😍😍

[@sanaznoormohamadi9250]

perfect special regards

[@shomusbiologyofficial]

You're welcome

[@AS-mm4pn]

What exactly did you mean by freezing the mRNAs?And how can you separate the 2 strands of a dsDNA?

[@mandisangema2568]

how do you know the complementary sequence of the gene i.e. the probe or bait?

[@masoomarazi212]

Sir plz a video on sts mapping tnk u.

[@shomusbiologyofficial]

Okay

[@kimlynnybaby]

thank you, you were extremely helpful

[@nithyaa5841]

Sir what about in situ hybridization using non radiolabeled probes??

[@pagalchori577]

thank u shomu sir ......

[@bikramroy3783]

Sir can you tell me which book it is present