In Situ Hybridization

This lecture explains about the in situ hybridization process and the role of in situ hybridization techniques in identifying specific DNA sequence.
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[@RahulKumar-mm5vm]

I have a question regarding dna sequence using FISH. "Is it possible to identify a particular gene sequence (for example - Amel Y) using FISH if there is lot of depurination (loss of adenine , guanine) in interested gene sequence ?"

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Helpfull for me all six lecture related to gene mapping......thanks sir .😊

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[@khushshah524]

I am presenting this technique tomorrow in front of my PI. This guy saved my life. I have a question: what does hybridization mean in HCR? Which step in this process is
called hybridization?

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[@rahishaaz4392]

Sir is FISH technique used for serotonin binding receptorss???
Plzz answer

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[@nehanasir3492]

How is the complementary strand to the target sequence removed before hybridization?
Because it may happen that during hybridization or cooling, the target strand again bind to the complementary sequence instead of probe

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It was very helpful bro ,thank you for making the video

[@aishasoliman8647]

really helped!! thanks a lot
I have a question:
If I have wild type and mutant models and I wanna examine the expression of specific markers Should I prepare probes for each markers for both mutant and wild type ?

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Hello, can you explain about detecting abnormal chromosomes such as 18,21, by FISH?

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[@kouapatrick3838]

Thank you Shomu! I would like to know how the 2 strands of the DNA is separated in this process? by heating the cell ?

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KOUA Patrick i think nick translation is used.

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do one about WISH

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Thank you. Plz how we can separate the strands ? in vivant

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Is it not same like southern blotting...?
What's the difference between these two..?

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[@totallyomi]

Sorry if this is a stupid question but how do we know the DNA sequence we are targetting in order to design the complimentary probe?

[@shomusbiologyofficial]

Dna sequence must be known prior to the experiment

[@sanaznoormohamadi9250]

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Plz add a video on MLPA.

[@AS-mm4pn]

What exactly did you mean by freezing the mRNAs?And how can you separate the 2 strands of a dsDNA?

[@KIRUBAKAR]

Bro what's the difference between colony hybridization and insitu hybridization

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thank u shomu sir ......

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Sir, is this same as southern and northern blotting techniques?

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[@mandisangema2568]

how do you know the complementary sequence of the gene i.e. the probe or bait?

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As i am pursuing PG.....wanna more elaboration of topics.......it wz lil bit helpful