FISH - Fluorescent In Situ Hybridization

Hey guys,
today I tell you how FISH works.
Cheers, Henrik
Instagram: / king_henrik_the_1st
Literature:
Bartlett, J. M. (2004). Fluorescence in situ hybridization. In Molecular Diagnosis of Cancer (pp. 77-87). Humana Press.
www.genome.gov/10000206/fish-...
www.neb.com/products/M0303-DN...

[@henrikslab]

Which topics should be next? Please make suggestions here:

[@harikabharadwaj98]

DNA fingerprinting

[@nbijetadevi1719]

Microarray

[@philippphilipp9832]

Bacteriophage typing method

[@darshanhegde7717]

Karyotype versus FISH versus microarray versus targeted exome vs clinical exome

[@shukuraopeyemi6670]

Qq%
1%q@a1@100

[@siyabendunus6443]

Thank u. Nice german accent ;-)

[@thetrollpatrol8799]

Why the wink

[@niels1318]

@@thetrollpatrol8799 ;-)

[@cfuenza4106]

Extremely awesome and relevant to the preimplantational genetic testing environment. Well done, clearly explained!

[@utrlohan]

Excellent work, thank you very much for your time and effort.

[@mariociencia12]

This is the best video about FISH that I saw on TH-cam!

[@deniseoliver-stergiou7842]

Wonderful easy to understand video! Thanks Henrik!

[@gabrielanunes146]

English is my second language and the way you talk very slow was just amazing for me, because I'm studying about FISH in my university in Brazil and I'm so proud of me to studying in English. By the way I have a test today wish me luck!

[@doodoobearlove]

Thank you totally clear my confusion! and right to the points!

[@user-mj4ur8yc7i]

really clear and helpful....solved my problem about why we used small DNA fragments ,thanks a lot

[@user-ek7cy2lx2t]

Nice explanation, You made the concept easy and digestible, thanks

[@deivibarci2093]

Thanks a lot :))) You just saved me 3 hours 🌟🌟 Keep up the good work !!

[@hollyashton7073]

Very helpful! have you done one of CISH?

[@Augetie]

This helped me so much thank you for explaining in such a calm voice and tempo

[@mixkopf388]

no, this video is shit

[@Augetie]

@@mixkopf388 it helped me lmao

[@anusharamdurga6943]

Really Thank u so much sir for this video ☺️ it helped me a lot to do my seminar ....God bless you 🙏

[@priyasaha183]

This video is very helpful to understand FISH easily

[@lexa5950]

This video was so helpful, thank you for your effort

[@Swati.ss567]

Very clear n concise... Thanks a lot really

[@nmz61]

Thanks, that was easy to follow and understand.

[@alaechda9056]

An exemple of how to design a probe for a specific gene will be nice

[@tanjiawen7467]

very detail and clear. thanks a lot

[@mohammedal-hammadi5085]

It's so helpful and clear video, thank you so much really

[@trivapatel1915]

Helped a lot thank you so much☺️

[@rosette_renah]

This was soooo helpful,,thank you

[@riamirto5002]

Thank you! Really helpful!

[@dr.qaisarjan2440]

Greatly well explained

[@kui635]

Kurz und knackig, danke!

[@santyadel6323]

That was very helpful, thank you

[@RahulKumar-mm5vm]

I have a question regarding dna sequence using FISH. "Is it possible to identify a particular gene sequence (for example - Amel Y) using FISH if there is lot of depurination (loss of adenine , guanine) in interested gene sequence ?"

[@thaliahurtadozegarra8516]

thank you very much for this video!!!!,I understand all

[@zaysanatomy]

So cool! Thank you!

[@meeromeer8812]

Thanks. I need to know how to figure out the used probes! For example: we have thousands of syndromes, do i have to make probes for all of them (especially if I have no idea about symptoms)?

[@brorjohannestidemandruud1870]

This is great!

[@anirudhmy4528]

Wow man very nicely done
It was very helpful for me Thank a lot.

[@henrikslab]

anirudh my thank YOU for watching it and the feedback!

[@sonalimali8160]

Very nice for understanding thank you

[@telugufunnymoji5564]

How the deletion site is known . And what confirms that probe is bound like how it gives signal after binding to dna

[@erkegirl]

This video was real good!

[@daenalund1613]

Very helpful thank you so much!

[@kubranuraydn5036]

very good video.

[@zglrd8938]

Does interphasic fish allow us to detect both anuploidie and microdeletions or just anuploidie?

[@priyankaburange5832]

Thank youu !!

[@tlechem9]

THANK YOU 👍👍👍

[@sangeethap5197]

Useful sir 👏

[@siniorashani]

Thanks very much ❤

[@sapthakathilakarathne242]

thank you !!

[@ashirt2100]

Thank you:)

[@lilbits661]

Crazy German scientist over here.
Thanks tho, it was very helpful!

[@yonglongd2990]

do you heat the cells/tissue up to 95 C? will the cells/tissue be destroied by such a high temperature?

[@sostyles14]

thank you

[@buddha7390]

Thank u sir

[@bushrazaman9882]

Thank you

[@Chelssums]

THANK YOU!

[@Vagolyk]

Never thought this is how fish are made.

[@arnotrihalder1237]

very helpful

[@tinomaster12]

Thankyou!

[@Federico-mj3si]

Thanks!

[@dortartakovsky7123]

How you can amplify the flourocent probe by PCR? it wouldn't increase the amount of the probe which is tagged

[@ameliac504]

THANKS! NEEDED FOR A PAPER

[@ravioli6301]

Is it correct that a ARRAY CGH is kinda a reversed FISH? U should def do a video about micro array especially the array CGH as it is also used for chromosomal DNA deficts :)

[@user-xx6kx7wi7w]

THANKS FOR YOU

[@ruanfamilytv9658]

2 Quick questions
1) how can the ligase seal the fluorescent nucleotides. They need to be bound first right? So my question is how do these labelled nucleotide bind? DNA polymerase will just randomly come along?
2) if i do the labelling before I amplify, how will the PCR be able to copy the fluorescent dntp?
thank you

[@Pearlblack6387]

in my opinion, ligase has some recognition site that recognizes the specific sequences and then it binds and causes ligation. DNA polymerase also has some specific sites that recognize first and then do polymerization. is this correct?

[@manishjadonrajput3095]

Thank sir explain mutations.types like this.

[@franciafenovavy6608]

After denaturation, why will the target chromosome will hybridized with the probe instead of rebinding to its original strand?

[@MinhLe-nw2zz]

You try to give the video more brightness it will be great if you do

[@rosedeleema4217]

Warm greetings. The short video is very helpful . But how to identify the specific complementary site in a chromosome . Or how do we know to prepare the complementary probe , on what idea this can be produced? If possible kindly send the answers. Thank you. Dr. Rise de leema .

[@henrikslab]

Usually you know the sequence of the complementary site since it is a gene you can look up in genome databases!
Best
Henrik

[@fabianperson]

Nice accent man!

[@meeromeer8812]

Thanks. Is it possible to tell which test do I have to do when I'm facing a syndrome?

[@henrikslab]

I would ask that a medical doctor!

[@englishspoken8168]

Cell fixation
Adding probe
Denaturation
Hybridisations

[@medicaleducation8978]

some time it gives false negative results because some time probe are not properly designed and sometime
there is change in annealing temperature ?
Am I right sir or any other reason of false negative result ?

[@herbalvision2134]

it's really interesting, please stay connected :-)

[@xaviasturm7248]

Gibt es auch ein Video auf deutsch ?

[@anisa7c]

how to make sure that the probe is fully hybridized not the complimentary DNA ? thanks :)

[@henrikslab]

First of all: Good question! My answer is based on this paper: www.sciencedirect.com/science/article/pii/S259000721830008X
If you make sure that the concentration of the probe is way higher than the concentration of the target dna (e.g. 10000 probes per dna strand) it should be way more likely that one of these probes bind after denaturation compared with the single sister strand of the dna
Hope you could understand my explanation!

[@zoew7872]

Can FISH detect mosaicism in an adult?

[@leilaaryan1575]

👍

[@englishspoken8168]

Fixation of cell et formaldehyde

[@idrisbagaruwagwarzo4363]

I really do appreciate you Dr Igudia youtube,your commitment towards saving human lives is steadfast ,all the knowledge I got from you and,the help with the herbal meds, I was recently tested negative of Hepatitis B infection,I’m so happy thanks doc

[@lexc8714]

So FISH can help in karotyping too?

[@henrikslab]

Yes

[@Meowmeows24]

What is the principal and the concept of the FISH technique ?

[@henrikslab]

It is to detect specific sequences on the patients chromosome (which are altered in disease). With FISH we make this sequence of interest visable using a "fluorescent probe".. this labels the sequence of interest

[@Meowmeows24]

@@henrikslab u help me , thank you SM😣🤍🤍🤍

[@sadahauch910]

Is Nick translation And FISH hybridization is same

[@henrikslab]

That is a good question, but here is the answer: So nick translation is just the process of getting your labelled probe. With this probe you can do whole process of hybridization then (FISHybridization).

[@taongamvula3313]

Where is the DNA that the probe originates come from?

[@henrikslab]

The DNA probes for FISH can be ordered by companies which will synthesize them. Is that what you mean?

[@anamoirangthem3817]

Sir I want videos for chromosome walking

[@englishspoken8168]

Fluorescent in situ hybridization

[@jaafar2562]

Did anybody learn this in grade 12? because in lebanon we do .

[@sylvia_forest]

Yes I do. I'm learning this as a new chapter.

[@jaafar2562]

@@sylvia_forest ah ok thx

[@englishspoken8168]

Add probe

[@englishspoken8168]

Denaturation

[@chemistrytutorvideos3801]

you sound soo german :)

[@whitetomato]

I don't understand... You never explained about the fish. Clickbait

[@sagarsanse8701]

Please hindi me bolo

[@earthworm-filledstomachbyc4254]

Seek Christ Jesus YHVH.

[@leonneumann2872]

sprich doch deutsch henrik