Hi, I just wanted to say thank you. I've been battling severe depression to the point I had to quit my job this year, but starting this plant hobby got me out of a really dark place. I appreciate you for not gatekeeping your knowledge, but instead sharing them to other plant enthusiasts like myself
"Why would your plants do all the work when you can instead micromanage their entire existence?" Haha, brilliant. I was looking to do this about 10 years ago for Aquatic Plants , but with the lack of info back then and supplies I decided to cultivate different exotic Mushroom species Now your making me get the itch back for the Aquatic Plant species as I have finally got some rare plants that I don't want them to be rare anymore to the hobby. Like yourself I started with the No Pour and Box with 100% Success. I think I might get back into it again as there's finally a club starting up soon and this would be a great opportunity to share genetics with plants.
Nice job on the video. I teach plant tissue culture at Grand Valley State University in Michigan. I love your t-shirts. I look forward to watching some of your other videos. I will be sharing your videos with my students.
I once went to a shop to buy a precision scale, mortar and something else which I dont remember. The clerk asked me "oh are you in culinary school?", I said "uh no, its for something else". Her demeanor suddently changed drastically and started treating me suspiciously.. I only understood the situation months later...
I used to go to the hydroponics store back in the 90s and they definitely treated me like a criminal, and I was lol they were spot. Now it's legal so same plant but no longer a crime, and the hydroponics store mostly markets to people who grow that. Crazy how times change , I didn't tho.
You act like GOD, but everybody knows, so why want his job!?? It's down perfect!! So to manage them, helps you manage them! They will never get out of control!!
Thanks Laur! Aluminum foil works well and is ubiquitous, no need for turkey bags. Sometimes autoclaving stacks of more than 10 tubs at a time can cause them to collapse on themselves, even without lids Tape is also cool but you can also toss in a paper autoclave strip too
Thankyouuu lol have been waiting for something easy like this lol reading Fry's my brain and I ask to many questions to myself lol this is soooo helpful 🤣😁
This is exciting. I'm a long time gardener and love propagation. I got into mycology during the pandemic and this is very similar to how agar is used there. I don't have a flow hood, but I have very low contamination rates with a filtered box fan and experience as a germaphobe. I frequently use pressure cookers and canners and I am still very much scared of them😂
I've been growing mushrooms for ~20 years. They are very similar, which is why I'm giving this a try myself. I'm not even all that interested in plants, personally, but when you already have most of the tools and experience necessary then you don't have any reason not to try something.
People have been pressure canning food since the 19th century. Pressure canners have a rubberized safety pressure relief valve that will blow out if an over-pressure event occurs. I love to pressure can food.
There's an asian market near me that sells Agar Agar for 99¢ for 25 gram packets. cheaper than buying the stuff the suppliers gouge you for. and it's the same stuff.
IDK who needs to know this, but pronouncing it "-kin-" instead of "-kai-" is a fantastic way to annoy immunologists. Also consider pronouncing enzymes with "-ayz" instead of "-ase."
I once did a guide on making no-pour agar on the shroomery forum, many years ago. It was almost exactly the same as what you do here :) What a blast from the past hah, we even have the same pressure cooker. That thing cost me an arm and a leg to ship to the EU.
Feel free to tell me more!? To go to class, is lick putting me in 1st grade. I'm passed that point. I never went that far, because it was better to have a job, and count on me!!! 😊😊
I use a oven, colloidal silver, my dirty room air and it works... must be the colloidal silver? Ever try that? I spray inside tissue container, bake it till it's 200 and silver has evaporated, then I add the media that has some colloidal silver mixed in.. i soak cutting in colloidal silver for 16 minutes and I also use a bleech mix separately
Sounds like it should work, keep in mind though that colloidal silver might cause stress in plants (if i recall correctly) and might be mutagenic in some cases. Cannabis growers actually use it to induce the growth of male flowers on a female plant to pollinate another female plant or even itself with its own pollen. That way you can get 100% female seeds later.
Hi i really really hope you see this and respond. 1stly i love your works and aspire to be just like you. I wanted to know if murashige and skoog is a complete medium for multiplication and growing or do we need to put some stuff extra like hormones etc.
I just found this channel and wow awesome and i never heard of tissue culture in my life. I am unsure how i never have, if i have it went right over my head! lol Have you tried this on evergreens like a yew or a juniper? if so what vid and if not be a great vid. I searched your stuff and binge watching now!
I am so happy that you have posted this! I worked with growing fungi in media way back in the 1970's. You are wonderful to listen to, since you know your stuff. But I really appreciate your sense of humor. Ah, those wonderful days of waiting for the autoclave to cool down so I could pour the Potato-Dextrose agar. Thank You and please keep at it!
We use glass petri's to pour media before it is autoclaved, and let it cool and solidify inside the sterilizer, we get less contamination this way. But this is for mycology I really don't know about contam issue on TC
I've been using mine for mycology for years, and now TC as well, and yeah. No trouble at all. My contamination rate to date is about 0.01%, and I'm about 100% sure those rare instances were because I was sloppy after the media came out of the pot.
"Accidentally" drop some of your hair in one of the jars and make one of those viral homunculus videos lol! I like to weigh out clay for my pottery and I always weigh my cornbread!
hello Thank you for the videos you make for us If I want to keep a plant sample for 3 months, then put it in a glass for tissue culture, what kind of hydrogel should I use? Thank you, if you have another solution besides hydrogels, tell me
Cool channel! Couldn’t you pressure cook in an old vodka bottle? I use to do this with my agar for mushroom cultivation. Then pour in a still air box or laminar flow hood. I would recommend making it in a glass alcohol jar then using isopropyl alcohol to spray down the containers. Could even use a few minutes of uvc exposure on them to kill even more.
Hey hi , I am really inspired by ur work but I have a single request for you that if u could use glass wares instead of plastic wares considering the global aspects 😅. Then u will the best teacher for PTC on this platform . I wanted to share this info as a request by ur inspired student.❤
Ask for a small scales and mention "its for use in my lab" the looks aee priceless... PH probes are great... make sure you keep the batteries fresh or they'll give incorrect readings, I use PH paper.
I mistakenly only used about half the amount of MS I was supposed to. Ive already transfered explants to it. Should I get rid of the extra media and explants or let them try to grow?
As far as I know, you pronounced cytokinins correctly. (All of my professors in the past have always pronounced it the way you did the first time, not the way that the AI voice pronounced it). Thanks for the cool video. Oh, 1 question why sucrose, and not fructose? Thanks!
Sucrose is the most commonly used sugar in plant tissue culture, probably because it is the most common sugar in phloem sap. But it's not the only sugar that works, for example, glucose is used in many protocols for micropropagation of _Quercus_ spp., especially during root induction.
Morning. What kind of growth could I expect on African violets without any hormones (just MS plus sugar, PPM, and Agar)? I was considering starting with only MS and sugar so I have a reference to compare to.
@@plantsinjars THANK YOU FOR YOUR RESPONSE. I AM IN THE CARIBBEAN AND IS WORKING IN TISSUE CLTURE, VERY NEW TO THE FIELD AND IS THINKING ABOUT DOING TH-cam VIDEOS.
Quick question! What would you personally use yourself?...8 Oz biocoupler or a 16 Oz biocoupler? Just looking to get one and didn't know which one to get...
I don't think the 16 oz would fit into a pressure cooker, but the 8 oz definitely does. I'll be using both in my livestream tomorrow night if you wanted to see a comparison side-by-side
would be nice to know what things like NAA are so i easily can look them up with out adding context to my search queries like "NAA plants" or "NAA tissue culture" you know?
I'm looking to clone medjool date palms. What is the proper way to add the tissue culture to the growing media? Here's a quote from an article, they don't describe the process step by step. . "Explants Preparation and Sterilization The offshoots of the Medjool variety were selected at the age of 2-3 years and their leaves were removed gradually from the bottom to the top until the shoot tips appeared shoot tips of 3 cm were excised with immature fiber 2 cm in diameter and then applied in antioxidant solution consisting of citric acid at a concentration of 150 mg/L plus 100 mg/L ascorbic acid. Explants were sterilized in sodium hypochlorite solution NaOCl at a concentration of 20% for 15 minutes with the addition of drops of 20 Tween and rinsed three times with sterile distilled water."
I tried mixing media before, but none of my explants callused or formed shoots. Though a few did eventually grow tiny new buds and leafs after 3 months.
Hi dear, I recently started watching your videos and I am very interested. I am still studying all the points. I can't understand how to weigh a very small weight, for example 0.2 mg/l...? Please help me
I've had private tours of 3 different county jails, 2 small town jails, and spent 2 days in jail in Montreal Canada and then deported back to the usa!! Thanks for asking.....
lmao at the casual "comment below". I've been to prison, not for drugs though. I've since got an IT degree and started a few businesses. I was quite misguided when I was young, unfortunately.
Laminar flow hood: box fan; glue or tape a HEPA filter to intake. Tape a garbage bag to the outflowing side. Now you can make shrooms without mould. Don't thank me; make the world better.
Hi, I just wanted to say thank you. I've been battling severe depression to the point I had to quit my job this year, but starting this plant hobby got me out of a really dark place. I appreciate you for not gatekeeping your knowledge, but instead sharing them to other plant enthusiasts like myself
Same here, stay focused ❤
"Why would your plants do all the work when you can instead micromanage their entire existence?" Haha, brilliant. I was looking to do this about 10 years ago for Aquatic Plants , but with the lack of info back then and supplies I decided to cultivate different exotic Mushroom species
Now your making me get the itch back for the Aquatic Plant species as I have finally got some rare plants that I don't want them to be rare anymore to the hobby. Like yourself I started with the No Pour and Box with 100% Success. I think I might get back into it again as there's finally a club starting up soon and this would be a great opportunity to share genetics with plants.
As someone who works in a lab but knows nothing about tissue culture, this is an awesome channel. Thanks!
Nice job on the video. I teach plant tissue culture at Grand Valley State University in Michigan. I love your t-shirts. I look forward to watching some of your other videos. I will be sharing your videos with my students.
Wow, thank you so much! I really appreciate it
I once went to a shop to buy a precision scale, mortar and something else which I dont remember. The clerk asked me "oh are you in culinary school?", I said "uh no, its for something else". Her demeanor suddently changed drastically and started treating me suspiciously.. I only understood the situation months later...
oh god
😂 thats hilarious
The reason I always explain myself in the lab shop.
I used to go to the hydroponics store back in the 90s and they definitely treated me like a criminal, and I was lol they were spot.
Now it's legal so same plant but no longer a crime, and the hydroponics store mostly markets to people who grow that.
Crazy how times change , I didn't tho.
@@artfx9 same, I just explain I'm reproducing the products from breaking bad
"Why would your plants do all the work when you can instead micromanage their entire existence?" 😂😂😂
Yea playing god is fun!
First of her video’s I have come across. That line sold me… lol
You act like GOD, but everybody knows, so why want his job!?? It's down perfect!! So to manage them, helps you manage them! They will never get out of control!!
You just introduced me to something i never thought I'd have an interest in. Now it's time to go deeper into the rabbit hole.
Thanks Laur! Aluminum foil works well and is ubiquitous, no need for turkey bags. Sometimes autoclaving stacks of more than 10 tubs at a time can cause them to collapse on themselves, even without lids Tape is also cool but you can also toss in a paper autoclave strip too
I love ur content ... It's more valuable than what they teach in college ❤❤❤
Great sense of humor very good introductory on the video keep up the good work😊😊😊!
That pressure cooker busting is hilarious 😂😂😂😂
Thankyouuu lol have been waiting for something easy like this lol reading Fry's my brain and I ask to many questions to myself lol this is soooo helpful 🤣😁
This is exciting. I'm a long time gardener and love propagation. I got into mycology during the pandemic and this is very similar to how agar is used there.
I don't have a flow hood, but I have very low contamination rates with a filtered box fan and experience as a germaphobe.
I frequently use pressure cookers and canners and I am still very much scared of them😂
I've been growing mushrooms for ~20 years. They are very similar, which is why I'm giving this a try myself. I'm not even all that interested in plants, personally, but when you already have most of the tools and experience necessary then you don't have any reason not to try something.
People have been pressure canning food since the 19th century. Pressure canners have a rubberized safety pressure relief valve that will blow out if an over-pressure event occurs. I love to pressure can food.
I love that shirt.....
I'll have to get one some day.
Thanks for the great video
i subscribe because is funny editing and good knowledge 😂❤
There's an asian market near me that sells Agar Agar for 99¢ for 25 gram packets. cheaper than buying the stuff the suppliers gouge you for. and it's the same stuff.
Your original pronunciation of 'cytokinin' was the correct version.
IDK who needs to know this, but pronouncing it "-kin-" instead of "-kai-" is a fantastic way to annoy immunologists. Also consider pronouncing enzymes with "-ayz" instead of "-ase."
I once did a guide on making no-pour agar on the shroomery forum, many years ago. It was almost exactly the same as what you do here :) What a blast from the past hah, we even have the same pressure cooker. That thing cost me an arm and a leg to ship to the EU.
I work in plant molecular biology your original pronunciation of cytokinins is what folks in our (uk) tissue culture lab say
Feel free to tell me more!? To go to class, is lick putting me in 1st grade. I'm passed that point. I never went that far, because it was better to have a job, and count on me!!! 😊😊
Here in U.S. labs as well. Because "cytokines".
Yeah like Cytokine storm. Don't trust AI blindly
Nice knowledge you share
Ooh, I loved the skeleton on the back of your shirt.
Thank you for your video, it helps me a lot!
I use a oven, colloidal silver, my dirty room air and it works... must be the colloidal silver? Ever try that? I spray inside tissue container, bake it till it's 200 and silver has evaporated, then I add the media that has some colloidal silver mixed in.. i soak cutting in colloidal silver for 16 minutes and I also use a bleech mix separately
I could stand to see a video on how you do that if you make videos
Sounds like it should work, keep in mind though that colloidal silver might cause stress in plants (if i recall correctly) and might be mutagenic in some cases.
Cannabis growers actually use it to induce the growth of male flowers on a female plant to pollinate another female plant or even itself with its own pollen. That way you can get 100% female seeds later.
What concentration do you use for the spray and in the media? Would love to try this if you have more directions! 🙂
The prison joke was funny 😂. I might have to try this out....the tissue culture, Not the drugs or prison stuff lol.
Can you buy premade stuff?
What would you use Callus formation for.
So cool thank you for the knowledge
Hi i really really hope you see this and respond. 1stly i love your works and aspire to be just like you. I wanted to know if murashige and skoog is a complete medium for multiplication and growing or do we need to put some stuff extra like hormones etc.
Love your way of talking
I think uts allegies, sirry
I just found this channel and wow awesome and i never heard of tissue culture in my life. I am unsure how i never have, if i have it went right over my head! lol Have you tried this on evergreens like a yew or a juniper? if so what vid and if not be a great vid. I searched your stuff and binge watching now!
Never been to prison, but commenting in appreciation of your humour! 😂
I am so happy that you have posted this! I worked with growing fungi in media way back in the 1970's. You are wonderful to listen to, since you know your stuff. But I really appreciate your sense of humor. Ah, those wonderful days of waiting for the autoclave to cool down so I could pour the Potato-Dextrose agar. Thank You and please keep at it!
We use glass petri's to pour media before it is autoclaved, and let it cool and solidify inside the sterilizer, we get less contamination this way. But this is for mycology I really don't know about contam issue on TC
Hi! Thanks for the content
Will glass jars with metal lids suit for the PC purposes?
Yay, finally people are starting to believe you can use the instant pot. I have been telling people that for years.
I've been using mine for mycology for years, and now TC as well, and yeah. No trouble at all. My contamination rate to date is about 0.01%, and I'm about 100% sure those rare instances were because I was sloppy after the media came out of the pot.
I was an Instapot-denier until recently
@@steve-adams Me too.
@@plantsinjarsI know. I watch your videos. I also use vinegar and sodium bicarbonate to adjust my pH.
@@justinschaeffer4364 I just use phosphoric acid. it's what plants crave anyway
plant nerd🌿🌴🥀🌱💚love it
"Accidentally" drop some of your hair in one of the jars and make one of those viral homunculus videos lol! I like to weigh out clay for my pottery and I always weigh my cornbread!
Very nice information
So did you have a specific question about prisons? What do you want to know?
hello
Thank you for the videos you make for us
If I want to keep a plant sample for 3 months, then put it in a glass for tissue culture, what kind of hydrogel should I use?
Thank you, if you have another solution besides hydrogels, tell me
Cool channel! Couldn’t you pressure cook in an old vodka bottle? I use to do this with my agar for mushroom cultivation. Then pour in a still air box or laminar flow hood.
I would recommend making it in a glass alcohol jar then using isopropyl alcohol to spray down the containers. Could even use a few minutes of uvc exposure on them to kill even more.
Can you try and clone woody material? Thanks great content!
YOU are an absolute nerd.
Another fantastic video , with great commentary and great direction.
Highly recommended. A+++++++++++++++
Great vid..👍
Do you have a link or more specific specifications on the container used in method 1 (Autoclaving container already filled with media)
Appreciate the videos, I do similar work with mycology and fungi, will give TC a go👍👍😊
Hey hi , I am really inspired by ur work but I have a single request for you that if u could use glass wares instead of plastic wares considering the global aspects 😅. Then u will the best teacher for PTC on this platform . I wanted to share this info as a request by ur inspired student.❤
Ask for a small scales and mention "its for use in my lab" the looks aee priceless...
PH probes are great... make sure you keep the batteries fresh or they'll give incorrect readings, I use PH paper.
I mistakenly only used about half the amount of MS I was supposed to. Ive already transfered explants to it. Should I get rid of the extra media and explants or let them try to grow?
Does the pour unter hood method also work if you wrap deli cups and lids into aluminum foil?
Yea you can use aluminum foil instead of the slow cooker bags
The apple dance
Hey, never thought of clones for plants, I taught myself.most of this stuff for mycologuy...
6:18 Who isn't excited about tissue culture? ❤
Early Drug-Dealers: "This is a Lab-Scale!"
Today's Lab's: "This is a Drug-Dealer-Scale!"
your right about the scales 😂 pocket sized for a reason. never touch anyone elses.
(no i havent)
should the container be sealed completely or slight loosen?
As far as I know, you pronounced cytokinins correctly. (All of my professors in the past have always pronounced it the way you did the first time, not the way that the AI voice pronounced it).
Thanks for the cool video.
Oh, 1 question why sucrose, and not fructose?
Thanks!
Sucrose is the most commonly used sugar in plant tissue culture, probably because it is the most common sugar in phloem sap. But it's not the only sugar that works, for example, glucose is used in many protocols for micropropagation of _Quercus_ spp., especially during root induction.
I was wondering if you have you ever used natural rgh's such as willow bark extract or other plant based hormones instead of store bought powders?
Nice 👍
sahy-tuh-KAHY-nin is valid (perhaps preferable) pronunciation. You were saying it right.
Thank you
Morning. What kind of growth could I expect on African violets without any hormones (just MS plus sugar, PPM, and Agar)? I was considering starting with only MS and sugar so I have a reference to compare to.
They can multiply without PGRs and you don’t need any PPM
Do you ever find the need to use the isothiazolones sold as “PPM” (plant preservative mixture)?
Nope
You're so stunning! I'm planning to propagate Aquilaria malaccensis trees by tissue culture, any tips?
Can you advise on where you got the poly containers? or where can I purchase some of them please?
The big ones are from Plant Cell Tech. The smaller ones are from a restaurant supplier, but I don't recall the name
@@plantsinjars THANK YOU FOR YOUR RESPONSE.
I AM IN THE CARIBBEAN AND IS WORKING IN TISSUE CLTURE, VERY NEW TO THE FIELD AND IS THINKING ABOUT DOING TH-cam VIDEOS.
NO PROBLEM GLAD YOU ENJOY THE VIDEOS
Wait, Im confused! Do I need to put cytokinens (BAP) AND auxins (IBA) BOTH in my media mix to grow anything?
Not necessarily, it really depends on the plant and the protocol
Hey there, fascinating channel. Have you tissue cultured a Morus Nigra before? They are super difficult to propagate.
Quick question! What would you personally use yourself?...8 Oz biocoupler or a 16 Oz biocoupler? Just looking to get one and didn't know which one to get...
I don't think the 16 oz would fit into a pressure cooker, but the 8 oz definitely does. I'll be using both in my livestream tomorrow night if you wanted to see a comparison side-by-side
@@plantsinjars than you. That's sweet.
Don't the magnetic stir bars get ruined by the heat
Is that a plastic pitcher you use on the hotplate?
I don’t use the hot plate while I make media
I’ve never seen this, does it work for cannabis??
For cannabis I wonder if you could just use Mantis Buffered Nutrients in a ratio of 5ml to a litre distilled water?
I literally have the same scale. I use it when I'm baking and I've ALWAYS thought it was a druggie scale lol
would be nice to know what things like NAA are so i easily can look them up with out adding context to my search queries like "NAA plants" or "NAA tissue culture"
you know?
I'm looking to clone medjool date palms. What is the proper way to add the tissue culture to the growing media? Here's a quote from an article, they don't describe the process step by step.
.
"Explants Preparation and Sterilization The offshoots of the Medjool variety were selected at the age of 2-3 years and their leaves were removed gradually from the bottom to the top until the shoot tips appeared shoot tips of 3 cm were excised with immature fiber 2 cm in diameter and then applied in antioxidant solution consisting of citric acid at a concentration of 150 mg/L plus 100 mg/L ascorbic acid. Explants were sterilized in sodium hypochlorite solution NaOCl at a concentration of 20% for 15 minutes with the addition of drops of 20 Tween and rinsed three times with sterile distilled water."
I tried mixing media before, but none of my explants callused or formed shoots. Though a few did eventually grow tiny new buds and leafs after 3 months.
What is the shelf life of this stuff
Hello. I have a dumb question about the sugar, how bad a Idea would honey substitute be?
I have not been to prison, but I am still commenting!
It is amazing how much you resemble my daughter when she was younger. She's a smart ass too. I don't mean that badly, just jokingly.
😍😍😍😍Waht did she said??
Hi dear, I recently started watching your videos and I am very interested. I am still studying all the points. I can't understand how to weigh a very small weight, for example 0.2 mg/l...? Please help me
Came from another video of yours.
How do you make 2% Vs 10% bleach? I'm confused...
I've had private tours of 3 different county jails, 2 small town jails, and spent 2 days in jail in Montreal Canada and then deported back to the usa!! Thanks for asking.....
Have you cloned any conifers?
Thanks for sharing YURE KNOWLEDGE...
Yu are LEADING EDGE .....for me at least
FOND REGARDS...
So you could do this to clone some olive trees for instance?
Yup! Pretty much any plant can be cloned in TC
@@plantsinjars Thanks I may have to give this a try.
You'r awsome
Pls can you do potato tissue culture please please 🙏 watching you from Zimbabwe Africa
I have a digital scale like that for years to weigh the acid dye for wool. I am innocent!!!😂
Looks like a postal scale
Those drug scales are great for nerd stuff, too 🙃
lmao at the casual "comment below". I've been to prison, not for drugs though. I've since got an IT degree and started a few businesses. I was quite misguided when I was young, unfortunately.
awesome :)
You are my Queen,thanks for the transferred knowledge. 🫡
i can't buy plant growth regulators in Chile :(
I really wish the stove to have "bye" when you turn it off, just because of that "hi" when you turn it on high xD
Laminar flow hood: box fan; glue or tape a HEPA filter to intake. Tape a garbage bag to the outflowing side. Now you can make shrooms without mould.
Don't thank me; make the world better.
Cut the bottom of the bag to make a tunnel.
Micropropagação de eucalipto? Alguem?
Below
Whatever the source, it's still a protocol as long as you do it the same way twice!
Facts
Dear can you make video on how to start tissue culture on small scale with all equipments required and resources required
I have one called "How to Build a Home Tissue Culture Laboratory" that should help