How to Grow ANY Plant from Seed
ฝัง
- เผยแพร่เมื่อ 7 ต.ค. 2023
- Please enjoy this synthetic seeds (aka biological boba) tutorial with a side helping of covid. This tutorial is definitely more intermediate-level but I have some beginner still air box videos coming out very soon so make sure to subscribe! To clear up some confusion in the comments: this is not a replacement for real seeds! This is a method to preserve plant genetics in a small space.
Check out the video that Francisco and I filmed on Plant Cell Technology's channel!: • I Had A Great Chat Wit...
/ plantsinjars_tc
Want to try tissue culture at home? Use code PLANTSINJARS for 10% off your Plant Cell Technology order: www.plantcelltechnology.com/?...
Supplies to Make Synthetic Seeds:
• Murashige & Skoog: bit.ly/3pMpAOr
• Sodium Alginate: amzn.to/3rB42pA
• Calcium Chloride: amzn.to/3Q1aMX8
• Micro-pipette: amzn.to/3Q4gkAf
• Pipette tips: amzn.to/3rAaPQh
Timestamps:
0:35 Preparing Solution A & B
3:29 Isolating the node
7:32 Making Seeds at Home
Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.
I have everything to do tissue culture. I've been wanting to get into doing it for some time now. Keeping mothers and clones of my favorite plant is a bit of work by its self. Thank you for the video.
Somewhere I was looking, they wanted 200 for the class but 2,000 for the cannabis class! Just like everything else that has for Mary Jane in the title, that price multiplies exponentially. Plants are plants, some easier than others, since some are woody, which would seem difficult, maybe not difficult but different
Doing the lords work with these tutorials, packed full of detail and great info
I am going to DC in 2024 to do a class with Francisco. I have been growing fungi since the mid-90s, so I have 80% of the materials that I need for micropropagation, but I think the class will be fun, not to mention educational. Plus, I want to meet others that are into this hobby. IMO, these techniques and the technicians who practice them will be in HIGH DEMAND as we reach out into the future.
The class is really fun and you'll learn a lot. I actually went to the most recent one :)
You sound like Bill Gates.
That is not a good thing.
See you soon 🎉
What fungi?! I'm interested in growing eatable wild mushrooms. They do however need their respective symbiosis with the tree/soil..
but why is he making such a point of the components being sterile while working in open air, and using a paper towel as a workspace?
This is what research is for. Trial and error will occur during these periods of research. This was informative thank you.
Ive been looking for a video of this process for years. Thank you Laur and Frankie!
Your videos are great!
Very informative and thank you for making them!
It is something that I have always been interested in. You are a big help for me to learn more about what it is and what it takes to tissue culture plants.
I love the manicure glass bead sterilizer you use! I recognized the brand and it honestly is exciting to know that the product works well enough for lab use!
Love your vids they are the best! The edits like the news on are amazing. Thx for the joy lol
Francisco is great. Seeing his lab was neat, especially the scope with the digital camera. Neat video. I liked that I even knew how to do a small part of it; the mixing is just like making gravy. One trick with gravy is first make a slurry by putting the powder in a little bit of liquid, and then put that into the rest.
Thanks so much for uploading a new video. I was withdrawing from your content. But now I get my fix 😂. Synthetic seeds! Omg brain overload. So much to learn
holy crap I've been looking for something like this for ages thank you so much!
That's fantastic, I'm learning so much with your channel
i have been wondering about these for a while, what they are and all of that, tysm for making this video
Wow, this is so awesome. It shows me how delicate yet tough life is.
Very Very cool! I had no idea this even existed until you brought this to us.
I am going to have to try this over the winter.
Holy shit. This channel is a fucking gem.
Really amazing process
This is really interesting. Thanks for sharing!
U are the reason why i am starting with tc ❤ thx
20% germination isn't that bad. If you compare it to the percentage of real seeds that actually grow (in the wild) it's very good rate lol
Well, in relation to the effort put into these synthetic seeds, 20% is extremely bad compared to directly planting the plants fresh from tissue culture. Why would you make these; just to better store them? You can simply keep some fully grown plants alive, from which you can take samples at any time.
These would be cool, if germination-rate could be drastically increased and the seeds would actually allow to plant huge fields from one donor plant. This only applies to indoor farming, since these mono-cultures would be highly susceptible to pests due to their lack of genetic diversity (see banana fungi problem).
@@haifutter4166 "Why would we make these?" yea for normal alone person it doesn't make sense. If people or companies are trying to spread their clones to others via sale or free etc then it makes sense. Eventually it'll get automated so the 20% isn't so bad in that regard... by that time we'll probably see an increase in the rate too
20% is pathetic.
@@Aussiehomestead1965 some seeds have like 3% naturally
i propagated all my plants using cuttings in an aeroponic cloner and allready felt like a scientist :D seems like i found a new method to try on my thyme and rosemary. very interesting!
FASCINATED!!!!!!! Thanks
Wow I'm impressed
I loved that face after he said the blade can really cut you up 😂
Cool, if these seeds had a better germination rate I could see this being a great way to cut down on some of the tissue culture in lab since a lot of what we have is just propagating old plates so we don’t loose the lines.
Great video! I would like to try this with auto flowering cannabis, since they are not clone friendly. Would be a game changer. 🍀
what a nice lifhack, exactly what i need for my project, thanks
Green screens and "Philodendrons" ;) Gettin' fancy!
Good protection for your seeds. Thanks for your video.
👌💎💎💎💎💎
You have a bright future in science!
"be the hot plate, you are the hot plate" hahaha amazing 😂
Channel your anger, and be your own hotplate. That is what I say.
very interesting and surprisingly simple technique. When he pulled out the Alginate i already had some idea what he's going to do but it was very useful for me to see precisely what plant part he is using for the seed. I think i might do that at home as well, thank you very much for the informations!
For anyone wondering the alginate and calcium chloride technique used is how they make popping boba in boba tea (bubble tea) 🍵
It reminded me of some of the "molecular gastronomy" techniques.
Yes! But with MS media 🎉
It's all recipes and cooking and cleaning glassware, etc..@@PlantCellTechnology
For anyone who don't fact check what they read on internet...
Bubble tea use tapioca, not alginate.
Alginate + CaCl2 is use on molecular fine cuisine for 'caviar/pearl' of different flavour.
So whose thinking of making a fortune with artificial salvia seeds by mail delivery?
Nobody. Buy shroom spores instead. They are legal for scientific purposes of course
@@ItemN9ne they require sterile conditions and do not produce salvinorin
have to try it
WTF.... I'm learning so much from your channel. I never even heard of this.
Hey! Your videos are amazing. Just a technical question on your videos, what camera/software do you use? Your background/green screen set up look really great! I've had problems in the past with background/greenscreens for my work from home set up, so I was just curious.
Thank you again for the amazing content!
You could put the jar in a pot of boiling water rather than the microwave as you can keep stirring.
Goals! Saving up and searching out equipment now! Thank you!!! 💚💚💚
AmScope has a variety of quality microscopes for reasonable prices. I have both a light and dissecting microscope from them and they are comparable in quality to the ones I use at work. I 3D printed a phone mount so I can take photos directly through the eye piece.
Awesome!! Thanks
Glad to see youre doing well! In April I cut my hand like you did 😂
good info, i just such the information in, hoping to do my own experiments, hopefully economical as well .. cheers :)
This is fn awesome.
For storage of the "seeds" just a cold environment needed? no light requirements? then looks like start growing it like any other normal seed?, very interesting. perhaps a good way to ship larger numbers of plants reducing possible damage during transport as well as size of package. Have you considered cococoir for a growing medium? the compressed bricks they come in can be rehydrated and autoclaved (at same time even I would think) to be sterile. I found cococoir to be the best substrate for rooting cutting and germinating seeds when playing around with hydroponics. Coco just seems to have perfect environment roots love and a neutral PH7. I used to just rehydrate the coco bricks with boiling water to steralize before using. Thanks for this, I had no idea this existed haha.
I know it would take a long time to make each video, but have you ever done a "growing a ___ from start to finish using tissue culture"? I'd love to see that.
I'm working on a few videos like that! They just take forever to make
For those of us on a budget, you can use a clear disposable straw in place of a micro pipette.
interesting use of molecular gastronomy techniques.
Hello, I love your videos. I have a question on the microscope. It looks like Francisco has one of the amscope binocular stereo microscope. Do you know what magnification would be suitable for tc work? I'm looking at one that has 10x and 20x magnification. Thanks!
Oh, edible wild mushrooms were something to multiply and grow by creating "seeds" out of them. However, they need the symbiosis with their respective tree/soil is essential! Interesting 🙂👍
When i grow up I want to be a hot plate.
😂
Beautiful charismatic and smart young lady doing science!
We don't have enough of you, keep on rocking babe!!!! 🎸
Thumbs up from Montréal
o.k. o.k. that fits into my actual insight on propagation by cuttings.
its the same, but on smaller scale, right?! does i work with any plant?
Did you have good luck growing them? Do they take longer than regular seeds to grow? Can they be contaminated, or would they have died before getting to this point?
I just saw a video that made me think of you and your efforts. A man came across surviving American chestnut trees that are seemingly unaffected by blight. First I wondered if these trees may have some natural resistance to blight. Second I thought perhaps they could be propagated via tissue culture. I know efforts are being done to alter the genetics for disease resistance. But, it seems to me propagating trees with natural resistance would be the way to go.
It would help in a sense, but they would be clones of the same genetics (or the diversity would be greatly reduced at least) so the trees propagated could wind up vulnerable to other threats on a very broad scale.
When you spread genetics far and wide, if they are all the same or very similar you will often find they’re all vulnerable to the same diseases and they spread it readily between each other.
I saw that video as well.
Sooo good ☺️
Serious question. would giberrellic acid help the success rate? is it used in tissue culture?
This is really cool. I can't figure out why you would do something like this (other than for fun and the cool factor). Seems like you could just plant the plant lol.
Maybe for additional ways to clone very rare expensive plants, for one
@@my2wins yeah i guess i just dont see the value really. The plant is already cloning itself. You just incase it in an sphere. Then "plant" that sphere. That is like taking a normal seed and instead of planting it you wrap in it another sphere and plant that. It doesn't really offer any benefits that I can see.
Are these live specimen only? Is this for cloning?
For what foods will this work?
Finally a honest realistic and fun channel about plants. I want to learn so bad.
Does this procedure need to be done from the tissue cultured plants or can this be done from sterilized plant material from a mother plant?
Andonstar microscopes for electronics are super cheap and would probably work pretty awesome for that
How about Calamus sp. LOL? Something a little more difficult than Monstera's etc.(that grow like weeds anyway). Cool channel. I'm a biologist Tissue culture, way to go! I've subbed too.
I wonder if it can be done with potassium algenate with greater success.
I am a student who interested in genetic modification in floriculture. I need to know about , is it possible to do color pigment of flowers change in every plants?
That s interesting philodendron
Not sure how I got here unless YT related it to gardening, but now I'm intrigued. What is the purpose of this? And why is it used? Thanks!
Now you have to try tissue culture, that's how this works
very cool! the "biological boba" cracked me up lmao. when i eventually get some equipment, I wanna try this!
How many jeers are they good in the breezer?
So what's the use case? What would you do with these that couldn't be done with a cutting, with the germination rates being so low and the process so tedious? Just why? x)
I'm not complaining, this was interesting, I'm just confused.
Obsessed with your channel, feel better lovely one!
Wait what? I had no idea this existed. That's crazy
I'm wondering if I could place some mycelium in stasis with this.
I´m surprised, how easy that is. It seem´s to be a method for everyone?! [ ... ]
I would subscribe, but I'm worried I'd become too obsessed with this channel, I'll just go now.
I don’t do tissue culture and find your vlogs so entertaining. Great job at production, filming and editing. Thanks.
thank you!
Living orbees! This is gonna sound weird but I kinda wanna take a bath in them lol.
Nice video
Without dedicated biology nerds like this, we wouldn't have the food supply we have today. Kudos nerds.
Do you have to use a pressure cooker to autoclave things or can you just use a steamer?
I don't really know the answer. Although a pressure cooker at 15 psi reaches 120c where a steamer would be 100c or less. So I assume the higher temperature would kill a broader range of microbes.
Make a video about how to get rid from viruses using tissue culture. There is a bit of info on the internet about it, but it is not clear enough
Once I get a microscope I definitely will try :)
What are you adding at 1:33?
The laugh @ be careful with the blades
Can these be shipped usig an ice pack?
Great Video!
(The sodium alginate Link in the discription is the wrong one 🙂)
OOPS thank you
Wow so interesting! So can you actually make a female cannabis seed this way?
how do these seeds store vs regular tc that needs to have medium changed every 4-6 weeks? btw your ig link is dead
HONEY WAKE UP PLANTS IN JARS POSTED
Could these survive shipping?
Can that really be considered synthetic? Is there any degradation of the DNA? And I wonder how long they can store.
I'm not shure what to think on one hand very cool never even heard of synthetic seeds on the other hand I'm very close to the earth. People seem to be cool people.
What is "ms media" at the beginning?
Pretty sure this would do well as a short advertising the long-format video
Where does the original cluster of seedlings come from? ( The ones you are harvesting the nodes from ).
From plants growing in tissue culture
@@plantsinjarswhy wouldnt you just grow those out then (instead of chopping them up and starting over from synthetic seeds)?
So, if you are already growing them in tissue culture, and they are more successful growing that way vs this pseudo-seed method, is there any "upside"to using this method?
(Super cool video... I really like the pseudo-seeds... just not sure why to use them)
mainly just to store genetics in a tiny space
@@plantsinjars If making a pseudo-seed could be mass produced without cell culture... ie, if the artificial seed actually improved germination of an actual "seed"... that would open up some interesting improvements. So, germination of an actual seed first, then coat it in the super seed, then make it dormant so that it could be planted later. I wonder how well that would work. Perhaps it would give natural seeds a boost?
@@johnwilder6486 Yes the begonias were in multiplication media. I've been using 1.0mg/L of BAP and 0.1 - 0.5 mg/L of NAA for them and they seem to love it. I usually move plants to new media every 4 - 6 weeks but those begonias were mostly large enough to acclimate to soil.
20% success?
FFS, this is stupid.
Cloning is easy (i had 100% success my first time) seeds store easily, in a small space, for DECADES.
This is propaganda to promote another Bill Gates initiative.
@@marcfruchtman9473 A couple of horticultural seed companies gave this a try back in like the early 80's as I recall.
You should make a video on how these seeds can be used to grow out a plant. Maybe title the video: "Lessons for luddites"
For germination are the synthetic seeds directly sown in the soil or are they grown in tissue culture first?
Directly in the soil
@@plantsinjars Thank you
I could see tissue culture being really useful for genetically modifying plants since you can start with a single cell and create an entire plant from it.
This is indeed an essential part of the process in nearly all species.
@@greggoralogia7401 I mean as opposed to modifying a germ cell or a series of cells in cluster after gamete fusion. Obviously all life starts with a single cell haha.
You could get a mature plant capable of bearing fruits or clones from a single cell with no seeds necessary.
Definetly, genetic engineering in plants is almost never in the germline though, pretty much only Arabidopsis and a few brassica species are amenable to that technique, everything else requires tissue culture. I would love it if it worked during fertilization in a wider array of species! It would make my job so much easier.@@Nanamowa
do any of the chemicals used increase the risk of epigenetic mutations in the new plant?
Since we're not using any hormones it would be pretty unlikely I think