It is better to weigh the tissue (the required amount) and then freeze dry it, either by adding liq. nitrogen or leaving it in the freezer for a few hours until it is completely frozen. In that way the tissue won't stick to the surfaces and such mess (1:10) can be avoided. And also, try not to discard supernatants in this way (5:23). Use pipettes instead.
0.25 M Tris-Cl (pH 8.0), 0.05 M EDTA (pH 8.0), 0.25 wt. % SDS, 1.2 M sodium acetate (pH 5.2), 50 μg / ml protein k enzyme is the composition of lysis buffer. Do share and subscribe if you liked the video.
Hello sir,
Thank you for your guidance.
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It is better to weigh the tissue (the required amount) and then freeze dry it, either by adding liq. nitrogen or leaving it in the freezer for a few hours until it is completely frozen. In that way the tissue won't stick to the surfaces and such mess (1:10) can be avoided.
And also, try not to discard supernatants in this way (5:23). Use pipettes instead.
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What is the composition of the lysis buffer that you are using?
0.25 M Tris-Cl (pH 8.0), 0.05 M EDTA (pH 8.0), 0.25 wt. % SDS, 1.2 M sodium acetate (pH 5.2), 50 μg / ml protein k enzyme.
lab safety pls
Cn u explain the composition of lysis buffer
0.25 M Tris-Cl (pH 8.0), 0.05 M EDTA (pH 8.0), 0.25 wt. % SDS, 1.2 M sodium acetate (pH 5.2), 50 μg / ml protein k enzyme is the composition of lysis buffer.
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How much the quantity of lysis buffer
Added approximately 1 ml of lysis buffer dropwise.
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When we add that supernatant into fresh tube into that we have to take phenol chroloform isoamyl alchol or into that first tube which is centrifuged
We add the PCI in the tube containing the supernatant.
Hope this clears your doubt.
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What can be the alternative of protienasek
Proteocut is another protein degrading enzyme that can be used.
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Bhaiya, ab is DNA ke sample ko ek bar microscope main rakhar dikhao na, aur achha lagega
Can you me protocol ?
Subtitles given in the video is the protocol
You can follow them
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you work like you are not professional. where is your gloves, This very local video
We do wore gloves where ever it was necessary. BTW thanks for watching and for sharing your views 🙂