Basically, crushing help to make smaller pieces, break cell-cell bonding and cell wall, detergent breaks cell membrane and nuclear membrane, salt separates proteins that are attached to DNA, and ethanol help to visualize DNA by making it insoluble (DNA is normally soluble in water) and finally the DNA here is seen actually lots of double helices strand are attached together (not a single double helix). a single strand can be seen in a microscope only. However the video is informative, you have prepared it nicely. Thanks
Sir this is incomplete experiment. It may also contain many other macromolecules like proteins and RNA. In order to remove these macromolecules we have to add enzymes like protease and RNase to get them dissolve and get purified DNA.
mix the DNA of mustard seeds with jack fruit or fruit which produced more seed so we got more oil like we mix the DNA of oil plants across the big trees like pipal tree or bargad tree or any large tree
The initial sample you have taken which you have taken are too little and in that amount of sample hod did you get that much of DNA. Definitely it will come in nanogram. I think your DNA got contaminated with protein and RNA.
mustard seeds DNA and people tree DNA small plants Oils gives tremendous amount of oil when Mixed with big trees so we got many tons of oil from tree every year
Basically, crushing help to make smaller pieces, break cell-cell bonding and cell wall, detergent breaks cell membrane and nuclear membrane, salt separates proteins that are attached to DNA, and ethanol help to visualize DNA by making it insoluble (DNA is normally soluble in water) and finally the DNA here is seen actually lots of double helices strand are attached together (not a single double helix). a single strand can be seen in a microscope only. However the video is informative, you have prepared it nicely. Thanks
This was better than the actual experiment tnks
Thank you so much
Nice explanation Thank you 😊
How to confirm the extracted one is DNA? Is there any simple methods to confirm?
@@gopinaththiru1238 By using spectrophotometer we check the peak absorbance value. It should be around 260nm.
This video also helped me for performing viva👍
Pls tell viva mai kya kya pucha ? I am having my practical tomorrow
@@inderjeetkaur5826 bro now I have practical tomorrow 😂
This video is very useful for me to do science expo in sri chaitanya school and i won first price it was very grateful to see this video
Thoda clear bolte toh jyada samajh mein aata.
Are you extracting DNA or chromosomes?
Usually the cell is not visible to our eye. Can we see the smaller DNA, sir?
If I'm not wrong , DNA obtained in above experiment is not from single cell ,it's from many cells of onion , so it is visible
Can we use sanitizer instead of ethanol
Yes we can
Sir this is incomplete experiment. It may also contain many other macromolecules like proteins and RNA. In order to remove these macromolecules we have to add enzymes like protease and RNase to get them dissolve and get purified DNA.
why just use the top bud of onion so it gives it's most potential of production
What is isolated is DNA or chromosomes?
mix the DNA of mustard seeds with jack fruit or fruit which produced more seed so we got more oil
like we mix the DNA of oil plants across the big trees like pipal tree or bargad tree or any large tree
The initial sample you have taken which you have taken are too little and in that amount of sample hod did you get that much of DNA. Definitely it will come in nanogram. I think your DNA got contaminated with protein and RNA.
What and where is this DNA used for ?
This was really helpful
Sir wecan use dish detergent for this experiment
Dr. Divya Teja Darvemula...which hospital are you working in? JIPMER??
Can we use powder detergent instead of liquid
Thank you so so much, i needed this for my experiment. Perfect explanation❤
What have you added before ethanol
Its not correct. detergent for cell wall?
mustard seeds DNA
and people tree DNA
small plants Oils gives tremendous amount of oil when Mixed with big trees so we got many tons of oil from tree every year
What is the function of NaCl , for osmosis or to separate protein
Mainly for osmosis
Thank you!
✨ACTUALLY✨
Feels as if the woman there is having viva 😂
Should we add meat tenderizer to the filtrate sir, please say fast, tomorrow I have exam
Lol, how was your exam?
Amazing very informative
Easy or simple method.. Nicely👍 desco about.. Extraction of DNA from onion...... Thank you sir nd mam🙏
which type of detergent?
Liquid detergent eg:- vim
Vim
Any detergent like dishwashing detergent or shampoo.
The explanation is very GoOd....🙂🙂🙂🙂
Isn't there any measurements???🙄🙄🙄
Wow
It helped me a lot
Mean while physics- tuta hua saaj hun mai😂
This solution should be heated
Right
what is role of Nacl
Thank uuuu teachers it helped me alot😍🤗🤗
Great explanation...thanks a lot
Pls confirm
Thanks
It is amazing 😍
Thank u
Outstanding ✨
Nice explanation .
mix the mango tree with oil tree like mustered seed plants so we got more oil as mango are full of oil and we got energy and food
I LIKED IT
Extraction of DNA from plant tissue, soil, water and microbes.
Who told you math and chemistry is the mother and father without DNA no mother and fathe biology is four father of math and chemistry
Boring
Who told you math and chemistry is the mother and father without DNA no mother and fathe biology is four father of math and chemistry
Actually DNA is not biology product it is chemistry product.