Plasmid DNA Extraction (Miniprep)
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- เผยแพร่เมื่อ 25 ส.ค. 2024
- ( www.abnova.com ) - Miniprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 1~5 ml of LB broth and the expected DNA yield is 20~30 μg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes Gigaprep, Megaprep, and Midiprep. More videos at Abnova www.abnova.com
could improve it by adding the functions and purpose of the buffer added... this could help viewer more understand... thx btw
Better late than never. 😂 They strip away all but the DNA plasmid rings inside the bacteria.
Would be better to desribe the function of each buffer and stripping reagent rather than the music in the background.
Thank you cause wtf now I have to find a different video
Ahhhh don't touch the inner rim of the lid of the tube!!! You will contaminate your plasmide -- neurotic mode off
Double filtering the elution step really helps!
Where can I find more of this music? I want it to be the soundtrack of my leif!
That A1 music though.
nice analyses
what is the composition of PD1 and PD2 bufferand the use of these buffers
nice video...plz tell the source of background music
so the entire DNA extraction takes less than an hour correct?
why does 'my lab' use incubation most of the time for this while you guys use the centrifuge?
Good tecnique.
2:10
what is pd1 buffer
Resuspension buffer
PD2 - Lysis buffer
PD3 - sterilization buffer
what is the temperature during centrifugation
Generally it's maintained at 4 degree Celsius
d video is truly helpful..thnkx
Shaky hands
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BIOSHOCK............