Plasmid DNA Extraction (Miniprep)

( www.abnova.com ) - Miniprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 1~5 ml of LB broth and the expected DNA yield is 20~30 μg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes Gigaprep, Megaprep, and Midiprep. More videos at Abnova www.abnova.com

[@eversonchin9163]

could improve it by adding the functions and purpose of the buffer added... this could help viewer more understand... thx btw

[@christopherleubner6633]

Better late than never. 😂 They strip away all but the DNA plasmid rings inside the bacteria.

[@christopherleubner6633]

Would be better to desribe the function of each buffer and stripping reagent rather than the music in the background.

[@ericawhite2865]

Thank you cause wtf now I have to find a different video

[@zahnpastacremetube]

Ahhhh don't touch the inner rim of the lid of the tube!!! You will contaminate your plasmide -- neurotic mode off

[@neurobiogirl]

Double filtering the elution step really helps!

[@skaalify]

Where can I find more of this music? I want it to be the soundtrack of my leif!

[@tatiananeal8754]

That A1 music though.

[@haryordeleholuyemi7351]

nice analyses

[@syed-wd8ib]

what is the composition of PD1 and PD2 bufferand the use of these buffers

[@rajkb47]

nice video...plz tell the source of background music

[@Jackmotoniwaqa]

so the entire DNA extraction takes less than an hour correct?

[@NanaBonciit]

why does 'my lab' use incubation most of the time for this while you guys use the centrifuge?

[@andiaprido2721]

Good tecnique.

[@user-xh4id8vd8o]

2:10

[@syed-wd8ib]

what is pd1 buffer

[@lifestyle12502]

Resuspension buffer

[@lifestyle12502]

PD2 - Lysis buffer

[@lifestyle12502]

PD3 - sterilization buffer

[@rajasreekonar8003]

what is the temperature during centrifugation

[@tiyasaguha5413]

Generally it's maintained at 4 degree Celsius

[@coolkolu]

d video is truly helpful..thnkx

[@lolmomz]

Shaky hands

[@ihssaneberramdane4900]

👍

[@Tonganpride1000]

BIOSHOCK............