DNA Extraction from Plant Cells Practical Experiment
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- เผยแพร่เมื่อ 9 ต.ค. 2020
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X: / thomastktung This video shows how to extract DNA from plant leaves using commonly available kitchen ingredients.
Do check out my other genetics related experiments by clicking on #GeneticsLab #ThomasTKtungnung above the video title. - วิทยาศาสตร์และเทคโนโลยี
MISTAKE AT 5:15 !
I meant to say, alcohols are comparatively much less polar than water with lower dielectric constant. So, the presence of an alcohol layer in the water, promotes the bonding of positive sodium and negative phosphate backbone, thus making the DNA more Hydrophobic and promotes its precipitation from the aqueous environment. Do have a look at these two links 👇 for more indepth understanding:
www.quora.com/Why-does-DNA-precipitate-in-ethanol
en.wikipedia.org/wiki/Ethanol_precipitation
Im looking to make poison ivy to not make people itch. How would I go about extracting the gene that causes it to produce the oil that causes allergic reactions? What is the gene that causes it? I tried growing it from cuttings to breed it with other plants but ended up with a face full of it.
After the extraction of the DNA how do I inoculate the other plant to achieve the transfer?
this video is so well explaned and so symplerfied, i understood it with out the audio on! keep up the great work!
You are providing really great content.
Thank you so much for your excellent rendition
Well explained following the A B C Accuracy, Brevity and Clarity.. Thanks
This is so well explained. 🤝🏾
Very nice practical with detailed explanation of each and every ingredients used ....Thank you
That's really amazing ❤️
Trust me in my school we have only done one thing and that is rattafication.... Thankyou very much for your video. This is the best video I ever seen.
Thankyou!!! It was really helpful
Thank you!!!!
Very Useful
Loved how easy this was to understand and follow along. I’m grateful.
very good content
Worth for watching thank youuuu
Thank you so much
Thank you
Well explained
Very nice way God bless u
Superb
Thanks
Amazing, subscribed!
Easy to understand❤
Mantap, makasih bang
Help full 😎
Great video..... What would be the variables - manipulating, responding and controlled
Love all your videos.. thank you! And subscribed 👍
That means a lot thankyou 🙏
@@TheSingtangpaScienceGuy I was scrolling through some meiosis stuffs for a student and came across your video. Its even more surprising to find out that you are from Manipur too.
@@boris2512 hi which district r u from
@@TheSingtangpaScienceGuy I am from imphal west.
@@boris2512 i just finished my Msc from MU. Finished my Bsc in 2012 from Bangalore. Spent 6 years upsc coaching in delhi.
Thank you
Pressed like button and well as subscribed with bell icon
Please make videos on Biology praticals of class 11 and 12
@@suchitajoshi9569 can u please let me know which practicals are included for ur upcoming exams? CBSE or State Board?
Would be neat if some day some one splices deep blue flower dna into a Daylily to make deep blue daylilies, they do not exist only different shades of purples but nothing true blue at least not on the petals and sepals.
can we use lemon juice to replace the pineapplejuice ?
why we used lemon juice in dna extraction??
Is there a Homebrew Method for compairing some Plants ? Would love to find out what some plants share and what not but looks like DNA sequencing is still a lab thing i quess?
For comparing the genome similarities or differences, we need a hitech lab setup. This video is , like i said, merely a rudimentary presentation of how we basically go about isolating DNA material from a plant.
👍🏻👍🏻👍🏻👍🏻👍🏻
Is it normal to insert juice or sap to other plant to develop GMplants?I have blueberry a temperate plant and lipote a tropical plant.both produce berries.one is blue one is purple.same appearance but different sweetness and tartness.i want blueberry to get the traits of tropical lipote so that it can tolerate warm weather but can produce bountiful sweet berries!
hello can I ask if it's possible to extract chia seeds? Thank you, Love your videos!
you can extract the genetic material from just about anything that qualifies as a living organism.
Can you extract DNA from a 20 year old leaf that was left in between 2 hockey cards in a binder for 20 years.
Is there any chance the DNA could be used to reproduce the original plant?
If anyone out there can do this that would be great.
what's the name of this type of DNA extraction?
Well explained video .. can I ask a doubt ! .. how can we identify & select a desired gene of interest from d organism for cloning?? Hope u reply
I think if you want to select a desired gene it is based on its behaviour
And you'll need to experiment with it multiple times till you eventually succeed
Sir,
I am isolating plant dna from leaf sample and getting smear in it...
Even after RNase treatment and purification of the DNA.
Could you please provide suggestions regarding the removal the smear...
Thank you in advance 🙏
Autoclave all pipette tips, work quick under ice, avoid vortexing, centrifuge at low speeds except for precipitation step
👍
I would try to mix the DNA of my watermelon and DNA of my dead tangerine
hello can I ask if I can use industrial ethanol and isopropyl that can be bought on supermarkets? I'm using this for our research, I really appreciate if you answer. Thank you so much for your contents
I'm not sure if supermarket alcohols are a 100% (or 99.9%) . If so i see no reason why you cant use it .
@@TheSingtangpaScienceGuy Thank you so much for this information, but is it possible to inject this DNA to other plants? btw, more power to you sir hoping for the success of this channel
@@kathleenclairemontefalco114 injecting foreign dna into another organism is the essence of Genetic engineering or Recombinant DNA technology. So much unexplored possibilities in this field. Wishing you too all the very best in your academics 👍🏾
@@TheSingtangpaScienceGuy I really appreciate your effort replying, thank you for your time sir
What can you do with the dna?
You can Restriction digest it and run on a gel.
I'm NEET aspirant and Biology student from India 🧬😊
Does the pineapple juice have to be fresh or can it be canned juice?
try canned juice and see if it works. Experiment! Why not! Although i have a strong feeling that the required enzyme in the juice would have been destroyed during the canning process.
I want to mix chicken with rose
Hello! Is it okay if i do not add meat tenderizer or pineapple juice? Will i get the same result?
Ypu can try
May I ask is there a way to do it with a smaller sample?
for smaller samples, i recommend you go for tha proper extraction protocols like CTAB method for plant materials. This is a very crude, High school level, demonstration purpose experiment, for huge sample sizes.
@@TheSingtangpaScienceGuy thank you for the reply.
How can I contact you?
Hi you can mail me titanbolt753@gmail.com or send me a frnd rqst on fb Thomas tk tungnung
@@TheSingtangpaScienceGuy I am curious to know your opinion of these APLGO products and if you would suggest them?
th-cam.com/video/C1O6qc3yTXY/w-d-xo.html
I slightly understand the concept and like to think they are nutritionaly beneficial. I would love to know your thoughts. Thank you.
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Are alcohols really non-polar???
Plz reply
Thanks for pointing out an Editing error. Apologies from my side. What i meant to say is that alcohols are comparatively much less polar than highly polar water. So the presence of an alcohol layer in the water promotes an even stronger bond between positive sodium ions and negative phosphate backbone of the DNA. Do have a look at these Links for more details. Hope this helped.
en.wikipedia.org/wiki/Ethanol_precipitation
www.quora.com/Why-does-DNA-precipitate-in-ethanol
@@TheSingtangpaScienceGuy Many Thanks Respected researcher for uploading such an easy-to-understand kind of lecture. I had just become confused at that point so I thought to confirm it. Heartiest Thanks
Would you happen to know how to “sex” a plant using its DNA? I would like to learn how to do this. Would you happen to know how to and if so could you please point me in the right direction?