🍄Liquid Mushroom Culture [Agar Series - Episode 2]🍄The best way to make Agar! Recipe Included
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- เผยแพร่เมื่อ 29 ก.พ. 2020
- In this Agar series episode, Andrew goes over the best tips and tricks on how to make agar for mushroom cultivation. Using Agar-agar, malt, and a few other ingredients, this is a very simple and basic agar to use for years. You can then use this agar to pour Petri dishes and slants for cultures.
If you missed the previous episode, check it out! It covers how to pour the agar after it's begun cooling.
Please leave any questions you may have below! And as always, don't forget to tell the Mossy Creek Mushrooms Team just how awesome, great and fantastic they all are! ~ Especially Ben who's creations makes everyone's job easier every day!
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Shopping List for Agar
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#Agar #growing #mushrooms - แนวปฏิบัติและการใช้ชีวิต
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Incredible teachings Mate, loving your work
A lot of information which is exceptional. Thanks for sharing as always.☝🍄👍✌
Excellent video, one of the better ones I've seen regarding this subject. Very helpful...Thank You !!!
And another great video thank you can't wait to see what patreon brings. Also stoked at the LC special you are running.
A nice in depth discussion on water agar would be great!
Thanks for this!
Great videos, your whole series of them. Thank you for doing this for us. I hope life brings you nothing but success.
Quality content thanks friend! Mush love!
Thanks a lot for translating your video
I strongly recommend the practice of saving excess grain and bulk-sub water for use with making agar. I have been very pleased with the results of this practice.
Good idea!
Looking forward to the cleanup episode
Thank you very much.
@12:44 that is helpful advice. “How to prevent rapid cooling and broken glass”
Have you ever heard of foam inserts for those flasks? They are autoclavable and reusable
Not sure if you covered this but if they are borosilicate flasks they won’t crack you can plunge them straight into an ice bath
I would like to see a solid grain spawn to substrate inoculation.
Great video. Can I use something else instead of soybean / fuel pellets? What about gypsum? Or coffee grounds? Thanks
Cant wait to have a go. Buying pressure cookers for this purpose is not easy here in the UK. We have to import them, as soon as I get mine I will be rearing to go with the aid of your amazing tutorials - massive thanks and hugs
Out of absolute curiosity, why do you have to import them? Government thing, think they are keeping your safe? (Gun control, knife control, pressure cooker control... Mind control)
@@neogeo8267 Probably availability, most likely no manufacturers for the one he needs.
@@neogeo8267 Can't speak for the OP, but in my country the issue is that you can't find any decent sized ones. They're all small 4.5 - 6 liters, which fit _maybe_ four 720 ml jars. Preparing any decent amount of grain spawn with a cooker that small is a major pain in the ass as it requires multiple batches.
@@neogeo8267 theyre probably imported into the US too if theyre made in china. But i think the issue is if there's a supplier that buys in bulk and resells them in your country. I remember watching how theres like only one company now in the US that manufactures enamel dutch ovens and cast iron i think as the video I watched said all the other companies went overseas. I forgot the name cause the product looked good, but ya most production is offshored of most things. Even the US military did a report saying how theyre reliant on china for inputs which is funny. The military is reliant on china for inputs to then face down china haha.
I'm using my oat soaking water with some peptone and LME in my agar plates. Its working great! I get strong growth within 24 hours!!!
Can you run down a simple recipe & all the measurements if you have a minute, please??
*Note to self:*
Add soy/sawdust pellets (or a toothpick, as in another video) for WOOD-loving mushrooms!!!
e.g., Shiitake, Enoki, Nameko, Maitake, Reishi, Lion's Mane, Pioppino, etc.
Hi. What agar are you using? is it regular one for cooking? and is it rye malt are you using? Thank you.
Is there a live feed today?
A mushroom video tutorial with NO THUMBS DOWN!
Although I'm very tempting to be the first to hit down (just a privilege to be first), of course, I will let this privilege to any other dumbass.
Thank you so much to share your knowledge, I'm new in this field, learning a lot and have my PC running right now!
Alex from Montréal
Well if you're new to mycology you should be watching this channel. Andrew is awesome and constantly learning as well as sharing with all of us. I've learned a tremendous amount from him as well as Eric @ Myer's Mushrooms and RT @ Earth Angel Mushrooms.
Watch and learn from the best cultivators.
Stop thinking you should give a cultivator of great mushrooms and tons of FREE knowledge a "thumbs down" on their video.
Learn grasshopper, learn...
@@ve-dr1fw Philly golden teacher is another great channel
I know this is old but they do make safe breathable lids for flasks now days...used for autoclave or Pcs
I use nutrition yeast plus malt and agar agar.
Do you find you have more contamination when you use nutritional yeast?
@@VickyG442 you sterilize everything in the pressure cooker so any contams should be gone if you did everything right
For financial gain....or at least for eating I should say. Are you growing decorative mushrooms? You sly fox. Thanks for the video. Making my first Agar today. Going to try black though.
Do mycelium grow at the agar agar after its done? Pls reply.
Is there a everything mushrooms promo code/discount that you offer?
What is the food coloring for?
Great Video! Did I miss this? Among the product links you show "50 mL Centrifuge Tube" and a rack for same. But are these even mentioned in the video? How are these used, even if not shown?
If you dont know how or what they're used for people tip they're not for you my guy😂
Are you using distilled water?
what is the difference between pda agar and malt agar ? Cost, contamination proneness...
I never had my media glass crack (Especially borosilicate).....and I usually use 40oz beer bottles (not borosilicate) that I toss when I'm done.
...but hey, whatever works.
Thermal shock caused the crack most likely, happens more often on metal tables, simply setting it down on a paper towel can prevent it, edit- later in the video now I'm seeing he said from cooling too fast in the pc, i dont use flasks so I'm able to put more water in so I dont worry about boil over or cracked glass from cooling, cooldown takes longer but it works for me since I pour a lot of plates at once and am able to set mine to hold at ready-to-pour temp so I just do other work and come back later in the day/the next day.
I like the hotplate idea a lot though, I've never fully trusted microwaved agar despite it being an established practice. Slowly remelting in front of the hood eliminates any (admittedly unfounded) anxiety
I need to know what kind of scale that is
hi mossy creek i want to know about commercial production of spawn. can you enlist the instrument for spawn lab
Amazing as always! Thanks for sharing your knowledge man.
In absence of a hepa filter, how would you recommend to pour the agar to prevent contamination?
In your previous agar video do you use regular alcohol or isopropyl alcohol to sterilize?
Thanks again for all the mushroom wisdom. I really appreciate all your content!!!
Isopropyl alcohol. If your using petri dishes a glove box is a good tool or you could use half pint wide mouth mason jars and you can mix the agar normally and pour into jars and then pressure cook. This is assuming you have no flowhood.
I can't get my 'new day' pressure cooker to stay at 15 PSI.... FML.... any ideas?
took a quick look @ your web site store , umm i didnt see any lions mane , or is it that you have a different name for it ? thanks . new to mycology so thats why im asking , i have grow out some cubes ...'''
adding the hardwood and soy pellets makes it grow faster??
How did I make you nervous?! I was on another tab!!!
Do you compensate for the soy bean and fuel pellets dust? Working with soy bean pellets comes with more dust than fuel pellets.
Probably not since he isn't adding that much.
Where can I just buy them lol
please give the full personal/small lab making/building tutorial stap by stap prominantly, ofcourse for me.
EVERY TIME I used tap water everything got contaminated... I use distilled and never had issues since
What if the mushroom isnt a wood lover what kind of sup can we use with agar and malt extract?
my lions mane is growing on malt plates
Nutritional yeast is mostly used
would decreasing the density of the plates and the nutritional content promote faster growth?
It’s possible, you can always experiment
This may have been asked/answered already, but isn't your agar already nutritious enough? Why do you add and what benefits do you have by adding the soybean and pellets?
The soybeans and pellets are likely the substrate that he is going to eventually grow these cultures on. By adding them to the agar in the beginning, the mushrooms will start acclimating to these Ingredients and developing metabolites to eat them. This helps make the cultures better at colonizing the future substrate. If you intend to use rye or bird seed as a grain, you could also add them to the agar mix for the same reason.
Is there any benefit in actually coloring the agar, except for it looking fancy and technological? I could think of better contrast in visually inspecting the petris, any thoughts?
Nope...you covered it all.
Actually you only need like 4 drops from the bottle (depending on how dark you want) , and sometimes the mycelium will make it disappear.
Color-coding different strains.
@@mariociani4918 he probably has well water
Have you ever posted a video that contains your steamer/sterilizer? I know its outside but I dont think weve ever seen it.... am i wrong?
You are wrong....he showed it.
Its in a feed trough IIRC...go back and look thru his vids.
What he did not really want to show was his electrical skills.
Please make a rice water agar ! Do you think it could be a good culture ?
Love videos you share us Andrew !
Trying to have a girlfriend to have a lifetime almost same you guy !
How do you get your pellet substrate to 60 % moisture content? Is there a formula at first? I am trying to zero in on 9 lb blocks using 4 lbs pellets and 6 lbs water. Reason for 9 lbers is cause i bought 1000 of the medium bags and anything over 9 lbs makes it harder to mix inoculated substrate. Also seen a video of a guy who puts two agar wedges on the same plate and then does a sectoring transfer from where two rizomorphs join together.
You just need to do the math.
You need your 9lb block hydrated @ 60%
9lb x 60% = 5.4lbs (your dry weight)
So now you have the dry weight (5.4lbs) which means you'll subtract that from 9lbs and get 3.6lbs (this will need to be the weight of water required).
5.4lbs dry plus 3.6lbs water = 9lbs for your block. This usually is easier in metric to scale out.
Good luck
Thanks buddy. I was just looking for the formula. Okay will do and give it a test run.
John can you help me on sawdust math in kg .i make 2kg block so what should be the water used and dry wait of sawdust
@@stevensylvester007 60% = 1.2kg dry 0.8kg water = 2kg block
Microwaving for sterility? Any recommendations
Buy a pressure cooker. And make soup with it when you're not spawning culture :^)
@@neogeo8267 🍄
@@neogeo8267 or beans......lots, and lots of beans. lol
We need direct tissue culture
caught ya you making money on the poo lovers out the back door andrew!!~~??? lol
Look it dat flicka dat wrist......................wrist
@8:14 I need to take a leak...9:12 my wife is taking a leak😮
Hi,
I'm Rajakarthik Rajarathinam from India.
I'm cultivating oyster and milky mushroom
I'm interested in liquid culture. Can you pls tell me how to make liquid media, is it just potato and dextrose?
How much cc of liquid culture is required to inoculate 1 kg of grain spawn. Thanks in advance
Thanks and Regards,
R.Rajakarthik
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