🍄Liquid Mushroom Culture [Agar Series - Episode 2]🍄 How to pour agar plates and slants
ฝัง
- เผยแพร่เมื่อ 25 ก.พ. 2020
- Agar can be confusing, difficult and messy! Learn how to pour agar plates and slants so that you can safely and securely, save your mushroom cultures long term or short term. In this first episode of the Agar Series, follow along as Andrew shows you how he pours the agar plates and slants that are used to store mycelium both short and long term here at Mossy Creek Mushrooms, the gourmet mushroom growing small business located near Knoxville, Tennessee.
Please leave any questions you may have below! And as always, don't forget to tell the Mossy Creek Mushrooms Team just how awesome, great and fantastic they all are! ~ Especially Ben who's creations makes everyone's job easier every day!
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#Agar #growing #mushrooms - แนวปฏิบัติและการใช้ชีวิต
![🍄Liquid Mushroom Culture [Agar Series - Episode 2]🍄The best way to make Agar! Recipe Included](http://i.ytimg.com/vi/BUEhn4M2goU/mqdefault.jpg)
![🍄Liquid Mushroom Culture [Agar Series - Episode 2]🍄The best way to make Agar! Recipe Included](/img/tr.png)
Hey Andrew fun fact. You can use your torch to flash the agar after you pour it. A quick flash will eliminate all bubbles. I used to do antimicrobial textile research and this technique used to help when pouring a few hundred plates at a time. I hope this helps and you see the comment. Best regards
I saw it. Thanks for the tip!
Bro you have got a talent like no other human being has and you explain everything to the T! You've made the top 10 mycologist on youtube! Imo
I love how raw and detailed he is. There is so much gold here for someone starting off.
Thank you for putting together this list of equipment and your techniques in such detail. It's much appreciated. And Much love and respect to you and to all the Mossback Family that made this happen.
Thanks Wayne! Same back to ya!
*Great work Mossy!* I really enjoyed personally watching this video very informative! Thank you :)
You are always helpful. I respect that you try to be as technically proficient as possible. Thank you for your work.
sweet content, can't wait to watch part 2!!
Thankyou for this content. Really looking into starting my own business and this information is golden.
Another great video, thanks very much for sharing all the little extra bits about your process very helpful for someone learning !
love it keep it coming !!
Thank you for the videos I'm just getting started!
Iam really learning a lot from Mossy videos..Keep up the excellent work
Nice I’m working to step up to this level .. mush 🍄love
More narrow is the correct way to say it. They being said, great tutorial and advice. Thanks!
Keep rocking it! Loved the tip about the labeling side of the slants thanks and MUSHLOVE
how mask water agr
have more confidence in your commentary bro, you do a good job with explaining things and including things that other people would leave out. keep up the awesome work!
Na, he's cool , he just assumes every 1 has common sense but not all of us are gifted lol 😆
Love the info/content. Keep up the good work!
Thanks!
Thanks Andrew.
Yes! Thank you! I know I asked yesterday and here it is! Really appreciate it man! Financial independence here we come
Hey Adam why do you have a dryer inside your lab? Super curious
@@chma0401Sorry I didn't see this. I'm Andrew btw, but I did indeed have a dryer in there. I don't now. It was going to be a bag shaker.
maaan im new to this and i love your videos
keep uploading 🔥✌✌
You should do an epoxy resin pour over that tile table you have there. Looks a bit distracting to the eye because of all the splotches and looks a bit porous. Nice smooth, clean epoxy would look great! Look up epoxy countertop if you don't know what I'm talking about.
More narrow brother lol you had me dying! Mush Love thanks for the education!
thanks for sharing your knowledge you are so awesome.
Thanks! We appreciate it!
Thanks for the water agar info.👍
You are very welcome.
Something cheap and easy to use as a container for making and PCing agar is an empty Pedilyte bottle (or the cheaper Walmart brand). DC Mak taught me this trick, and it really works! The bottles are PP5, and hold up very well to being pressure cooked. Before I had my media bottles this is what I used, and they survived tons of PC cycles without melting or deforming.
Just a tip for some of you beginners that are on a budget. Theyre like $3 each, and you get a drink out of it too!!
Thank for the info and taking the time to make these videos. Very informative and well done. Agar plates and slants can be seal up tight and don't need gas exchange? Thanks again !!
dude i will definitely try water agar to clean up my strain! thank for that excellent explanation!
Glad it was helpful!
love it 🍄❤️
Thanks
Hey thanks for this.
I came here because this video was shared by Alan himself so I thought it must be good. It is good.
I have two questions:
How long do you PC your agar?
What temp do you pour your agar?
I ask because I have some condensation issues on my plates, specially on the sidewalls and the droplets there then slide onto the agar and slosh around.
Thanks!
Professor Mossy, Love you and love your video’s. Personally I have had very good results with a plastic pitcher for pressure cooking and pouring agar. But I see the benefit of being able swirl and stir. For centrifuge tube slants, I fill the tubes and then pressure cook. One less potential point for contamination. I can also add a little piece of wood pellet and insure each tube gets the same amount.
Thank you! I appreciate the time an effort that go into each one.
Are your caps loose on the tubes are sealed tight when you're cooking them?
Caps sealed tight. Pull out of pressure cooker after pressure back to atmospheric. Prop at angle till cool/solid. Then I open in front of flow hood, pour out condense, and add a piece of colonized agar. My only contamination was when I dropped a cap and put it back on the tube.
thank you !!
Thanks mate
Do slunts needs gas exchange? Can you close them air tiet?
Thanks again
Love the content Andrew could I put in a request for a info episode of the water agar plates making. Gonna have to order those larger tubes brilliant.
Sure thing, I'll add it to the list. It'll probably be a short one.
Don't really need a whole video for that. It's the same exact thing as his other agar videos, just a different agar recipe. 20g of agar agar per 1000 ml of distilled water. That's all.
Pro tip,I put a 1 inch stir bar in,and it helps keep air bubbles out,while stirring it up
Interesting, so putting a stir bar in there helps keep bubbles from forming while I'm hand swirling it?
@@mycominer same basic principle, it just mixes a little better,I set on stirrer,and hand shake it like you do before a pour
Please make a video on the construction of mushroom growing room from A to Z.
Good Video....I would have thought the next, would've been the first.....lol
We enjoy being a bit different! :)
Great video :)
Thank you!
1:20 A PTFE lid eflask in a microwave is quick and easy. You can pump out a 1000mL flask (if it fits) in less than 5 minutes.
I like to pour with a freshly washed long sleeved shirt on no matter what time of year or how hot it is.... idk why.... not really sure if it makes a difference, but I also ended up shaving my head completely bald for this, so I'm just a little overboard. 😆 I was just wondering how to get less bubbles, and now I know, thanks. I got some really good tips from this, thank you.
I use a 1000ml media bottle with a magnetic stir bar in the bottom and place on my stir plate for about a minute to help blend the agar and lift any sediment from the bottom of the bottle before pouring.
What is the procedure for reusing petri, can you pressure cook them already poured, as a finished product. Very informative. Ty
thanks for the video. you need a sous vide setup to keep your agar at the perfect pouring temp.
Great idea!
Boy!
:) hella cute
You just saved me a lot of aggravation.... I will forever use jars of water as a temperature buffer with my glass flask now. I also have a Nalgene flask.... only thing with it is that I can't use the heat on the stir plate with it.... but it sterilizes fine
They make a spout that measures shot pours would that increase the chance of contamination?
flame the bubbles with a quick spritz from a blowtorch and they'll pop. works for custard anyway.
Enjoy your content man. Can I ask, what is the benefit of adding coloured dye to the agar mix?
Makes it easier to see development in the dishes in my opinion.
So i think a video idea is about pressure cooker maintenance. The seals wear out and sometimes they dont pressure as fast as when their new. This is not for me but for those who are new to pressure cooking. Models that they might wanna look at and buying a back up seal.
Sounds great! Added it to the list! Thanks!
How long do you let everything cool before opening your pressure cooker and begin pouring? I poured some that was too hot and my plates fogged up
Out of all the cultivators Ive been watching, your mushrooms look the best and you have the best mentality. I fell out of my passion for art after getting my degree for illustration, due to it just not being fun anymore and the whole culture behind the scenes. Ive been wage slaving for almost 10 years thinking I wouldnt find another passion. I grew some mushrooms and just fell in love with everything about it. Im trying to put together a business plan and Ive been trying to figure out just how many pounds I should aim for to start. How big of a space did you start with and how many pounds did you start growing before trying to sell them?
Just start man, there's a decent learning curve in getting your grain spawn prepped properly, and learning how to setup a grow room for proper air flow and all the little techniques to get certain strains fruiting properly. Just go for it, you gotta start somewhere, just remember you have to think of it as a business, loving what you do is important, but if you put that aspect in front of the business you're most likely going to fail.
Im new. Following you closely. The part im missing is getting spores in agar. Am I a cc or 2 on petri dish from a bought syringe?
Does the additive (sawdust, etc.) actually have an effect in agar that way? It looks like it has all settled to the bottom by the time the agar sets, which puts it out of reach of mycelium growing on the top surface of the media.
28:35 You mention that they get very few replications, is this to avoid senescence?
Ever hear about adding 5% glycerine to freeze liquid cultures in test tubes for very long term storage?
SUB'd !!!! Thanks for the Video - quick question if you might? getting into the hobby. I've done PF tek with some success. Tried to go giant auto mono-tubs and it just stalls when I go bird seed to bulk substrate with manure, but it doesn't really contam till week 2-3 when I'm poking at it. I think it might have two much FAE because it's a bit dry when I give up, but I would think the healthy seed would at least try to spread. I've increased humidity and still didn't see it trying to colonize the substrate - It just gets fuzzy but doesn't spread. Any tips? I'm going to start over and while getting experience with agar, > going simple mix for bulk and reducing FAE to close to zilch. I have a flow hood like yours and threw a few grand at this so I'm motivated to get it right. lol Thank you - especially if you can find time to respond? lol
thanks for great content ..you said you were going to go over recipe either I missed it twice or you never gave the recipe for agar
This Origami the boy showed is a funny toy!
Thanks !
Thank you for watching!
@@Mossycreekmushroomsofficial Awesome videos✨, very educational ! 🤗👍
1K 🤘
great video.... but, this is episode 1, correct?
the conflicting titles messed me up
Hey man did you not wrap the plates?
Hey buds do you do consulting or room design and set ups?
We do actually. We have a page on our website about it, but it's pretty easy to schedule. We do it remotely and on site if people need us to.
how to you prevent boiled over agar I Kept getting mine boiled over in the flask and it end up with half. BTW your fingers are all over the place ie on the flask lip, inside of the petri dish.
Quick question that I would be very appreciative on reply. What’s the best temperature to have agar out at? I live in a very hot climate and wondering on cure times after you pour. 🙏
You want to pour at 117 degrees mate at 107 it begins to gel up quick
I pour at 140 F (PGT advised)
Any particular reason you don't use glass petri dishes? Obviously, you wouldn't use them for the cultures you sell and ship out since they're pricey, but seems like they would be a good long-term investment for internal use. You can reuse them forever and pre-pour before sterilizing, so less risk of contamination.
@@justinlangley9529 "the added work or cleaning and sterilizing glass dishes is yet another expensive add on."
There is no "added work". Cleaning is just dumping out the current chunk of agar (you're going to sterilize the whole thing afterwards anyways), and sterilizing pre-poured glass dishes is the same amount of work as sterilizing agar in a separate flask and then pouring into plastic ones.
"Dont you hear him speak of 'poverty'?"
Sure, I also hear him speak of constantly having to buy new cases of 500 plastic plates that cost $300 a pop. If anything, buying glass petri dishes would save him tons of money in the long run, which is the main reason I suggested them.
"Handling glass with gloves x10,000 times a year is another reason"
Honestly, don't get this one. Is handling glass with gloves worse than handling plastic with gloves or something?
"Plus the man is a breeder and creator of new commercial strains. In other words...LOTS AND LOTS OF DISHES!!!"
Which would be another reason to buy lots and lots of glass dishes once and reuse them, instead of buying lots and lots of plastic ones over and over again ad infinitum.
@@otacon1024 I agree with what you just said, I have all plastic.....and some reusable autoclavable ones still going strong (none broke yet) after 25yrs. They are more costly, but VERY ECO friendly compared to the disposables.
Honestly man, the biggest reason is because I use pressure cookers and not a sealed autoclave. It wouldn't be a kindess to me to switch to glass right now. One day I'll have the infrastructure to go back to glass, but today is not that day.
Are you close to knoxville
best mushroom channel not gonna
lie
Dang the rhotic dialect of the scots-irish
Water agar. Thank you
Subbed for Aegishjalmur.
Now is there a difference between the black pearl oyster and black oyster?
Yes, as far as I know. I've never grown out the black oyster before though.
Could you fill the Petrie dishes first with unsterilized agar, then sterilize them in the pressure cooker?
This is an old question, but just in case you're still interested or for future readers, the answer is no. Well, not with petri dishes. You absolutely can do agar work like that, though. It just takes a few modifications to the process first. What you'd want to search for is 'no pour agar teks', and you should find plenty of info.
It's a great intro to agar work IMO, and helps demonstrate how easy and simple working with it really can be.
Panther Creek? Are you in NE Georgia by chance?
Not too far, we are in East Tennessee
New vidd!!!
🎉Celebration Time! 🎉
(New to shrooms) ignorant question ahead: What's the purpose of the poly fill? Why is aluminum foil alone not sufficient?
I'm sure you've figured it out, but polyfil is used for filtration. You can use Synthetic filter disks, double layered tyvek, a few other materials, but it's just to keep any contaminant out of your media.
lol both your videos are called episode 2.
Had to use the thumbnails to make sure I was on the right one.
Ja tego nie panimaju. Pracuję na agarze w salonie, oczywiście dezynfekcja 70%izopropylem, rekawiczki itp.. Ale zadnego SAB, zadnego floowhooda, i jedyny contamination to 1-2 na 25-30 zrobionych petri dishes.... To duzo czy mało? Bo mi to wcale nie przeszkadza. I zaszczepiając /klonując czy coś jest taki sam waste. Na 25 gotowych, strace 1-2 przez zarodnik plesni. Tyle w temacie xD
So I know that Agard dishes are disinfected with a gamma radiation but when you’re done with the dish, can’t you sanitize it and sterilize it and use it again
Wait if you hate yeast why do you used it in your LC mix is the yeast spent by the time it comes out of the Pres cooker?
The pasteurization kills it off, i beleave he said in a previous video, its cause he sees it as a good supplement for his fungi, like a multi vitamin.
It may also have to do with preventing enzyme blindness(like adding some masters mix), and promoting his fungi to express/strengthen enzymes that would allow it to fight contamination when in production.
@@TheLoiteringKid Thank you very much
@@adamanderson8149 I use any kind yeast (beer, bread, hulls)...its all dead after sterilization.
more narrow?
7:51😂
The chemistry nerd in me is pulling his hair out over the technical description of erlenmeyer flasks...
More narrow works? Hahah
✌👍🤙🍄🙏
Can i ask why the pertri dishes weren't sterilized?
They're sterilized pre packaging, irradiated at the facility that makes them, that's why he had to tear it open before he started.
How many ml are your flasks?
@1:06 1L
Ty...very observant
"Big fat man hands"
if you use a magnetic stir bar and plate you wouldn't need to swirl it
Don't overcomplicate it.
Think about what would be involved with that.
Yes, but then I would need to reset it on the stirrer every time. That would just slow me down and make it more complicated.
Why do I use pellets? So I can see through them through the disc. 06:00
Me- WTF
Me
I did a test with agar plates using dye vs no dye and all my cultures grew better without the dye. Only thing different was the dye. I will never dye my agar again.
You’re vary handsome
Sponsored by the Erlenmeyer family
i didn't know you could use liquid nitrogen to store mushrooms. You might could find save some money by buying some liquid nitrogen tanks from select sires or other livestock organizations. Our breeding guy has a liquid nitrogen tank to store semen we've bought and then to breed heifers. You could store a lot of strains of mushrooms in a tank like that.
I bought some spores from a Drugstore online
FROM
TRIPWITH_JEFF
in
instagram..
The repeating texture on his door is bothering me
more narrow
Why is the music so fucking loud?
more narrow. sorry.
Strictly out of love man it’s pronounced aw-ger not A-garr
Never, I refuse still! My appalachia will live on! ;)
that's just like, your opinion, man - the dude
It's recognized to be pronounced either way. Either with long A-gar, or as AH-gar.
Aw-ger, honestly never heard that before, I've heard Ay-gar and Ah-gar.
In England it's pronounced A-garr.
Thanks mate
Do slunts needs gas exchange? Can you close them air tiet?
Thanks again